LOCUS       NZ_CP125144             3511 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 324 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP125144
VERSION     NZ_CP125144.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790405
            Assembly: GCF_037477835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 43x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477835.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:23:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,808
            CDSs (total)                      :: 5,684
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 54 of 142
            Pseudo Genes (incomplete)         :: 86 of 142
            Pseudo Genes (internal stop)      :: 22 of 142
            Pseudo Genes (multiple problems)  :: 17 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="324"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            join(3482..3511,1..468)
                     /locus_tag="P4G14_RS29025"
                     /old_locus_tag="P4G14_28980"
     CDS             join(3482..3511,1..468)
                     /locus_tag="P4G14_RS29025"
                     /old_locus_tag="P4G14_28980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            475..690
                     /locus_tag="P4G14_RS29030"
                     /old_locus_tag="P4G14_28985"
     CDS             475..690
                     /locus_tag="P4G14_RS29030"
                     /old_locus_tag="P4G14_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1085..1267
                     /locus_tag="P4G14_RS29035"
                     /old_locus_tag="P4G14_28990"
     CDS             1085..1267
                     /locus_tag="P4G14_RS29035"
                     /old_locus_tag="P4G14_28990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            2463..2804
                     /locus_tag="P4G14_RS29040"
                     /old_locus_tag="P4G14_28995"
     CDS             2463..2804
                     /locus_tag="P4G14_RS29040"
                     /old_locus_tag="P4G14_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2205..2378
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1717..2238)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..806
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="9.340000000000001e-172"
     Typing          2795..3510
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="5.84e-160"
ORIGIN
        1 ttcgaggaga aatcgaaagc acagcagcag cttaccgaag agaggctgaa agccgcattc
       61 agcaagcacg agaacgttgt caaatcggaa ctgaacgcaa gcgcgaagag aatcaacgac
      121 gccatcagcg cccacgaaca ggggatgaac gcggcgatgc agtcgaaccg gctgagcgtg
      181 ctgcgaatgg tcgggcggac gtggctgacc atcaccatgg tgtcagggct gctgttcgcc
      241 agcctgagcg gggtgctgtg gtaccagggg aaccggatag cggcgaacct ggcggaaatc
      301 aggcagcaga gagatacgct gtcgaaactg cacatgcaga cgtggggcgt gacctatctg
      361 gagaaccgca acggacggtt tctggtgctg ccggagggga tgaaagccga aacgggccgg
      421 acggtggaca acaagacgcg gaacgcggtg aagctggtac gggagtagag cagaatgacg
      481 gaactggaaa aacaattgct gagcgcgtta gagcagctac agcaggacta ctcgagaagg
      541 ctggacgagt gggagagcgc cttcgaggaa tggcggaaga tgtctggtct tatgcaacgg
      601 gagaacgcgg cgctgagcga gcgcgtgtcg ggcttgagca ggcaggtgca gagtttgagc
      661 gagcagctgc gccggttatc gacaggctga atgagattga gcaacagcga tacgggcaaa
      721 caacggaaaa ggaaccagag aagacagcag atctggtacc ggaacggcaa cgcacattat
      781 ctcatggtga cagggagtat ggagactgat aaacgcccgt ctcaggtggc ctgagcgtga
      841 cggggatcat ggccattttc tgcgatttca gcacacagag agtctttttt aaccgaaatc
      901 accggctatg cagtgatgtg aataagtgat gcatatgcgt aaaataaatc aggtgcgttt
      961 ctggtggttt tcggtgtact tgccaccacc ttttgcccgt cagccatcag aaacgccctg
     1021 agtgcctccg gcgcggtgcg ggtaagagat gttatgttta atgttgataa caggagtaag
     1081 agtgatgctt acatttattg gtgtagtggt agtcgccgtt attgcattta aattatttat
     1141 gctttttttt agttcatccg aatctgccgt cgaacgtgct acccgtaaat atatggagaa
     1201 ccctacgagt gctaactata aaataatggt ggctacacgc atgagaaatg agaagaaaag
     1261 agggtgatat atgtttgagt ttattggttt tttattttta cttttggtgt gttatgtctt
     1321 tatacaaatg gttgctgtca gggtgtttcc tgaatatgga gtacggaaag cggaggaacg
     1381 ataccgtcga aatccatcag cagaaaatga ggatctgtta tggagagcta gaagtcgtat
     1441 aactaaacgc aagtaatgtt tcctaagtgt gttgataggc aaacatggtg ggagttacta
     1501 ctctcggagt taatgatctt cttgtgatcc tgtcgtttca gtcgtaatct cttgctctga
     1561 aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtctgtt ggggaactgc
     1621 ttaaccaggt aactgggtat acgagaccgc agataccaaa actgttcttt cagtgtagcc
     1681 gcagtcaggc ccatacttca agacctctga atctctctct cgtatatccc agttaccagt
     1741 ggctgctgcc agtggcgctt tgtcgtgctt tctcgggttg gactcaagat gatagttacc
     1801 gggtaaggcg cagcagtcgg actgaacggg gggtttgtgc atacagtcca gcttggagcg
     1861 aactgcctac ccggaactga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg
     1921 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccgccagggg
     1981 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
     2041 tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggcttcgcc gcggccctat
     2101 cgcttccctg ttaagtatct tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc
     2161 catttccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga
     2221 atatatcctg tatcacatat tctgctgacg cgccggtgcc gccttttttc tcctgccaca
     2281 tgaagcactt cactgacatc ctcatccgtg ccaacatagt aagccagtat acactccgct
     2341 agcgctacgt gactggttca gggctgcgcc ccgaaacccc actgacgcgc ctgcggcttg
     2401 tccaaccccg cgccgaccgg gaaaggcttt cccgtccgtc acggcgttat caggaggcga
     2461 ttctggcaga taaacgcaac aagatgctca cgatgtgggt gacggaagac gaacaccggc
     2521 ggttgcttga gcggtgcgac ggaaaacaac tggcggcgtg gatgcgccag acctgccttg
     2581 atgagaaacc gacacgcgcc ggcaggcttc cctcgatttc gcccgcgctg ctgcgtcagc
     2641 ttgccggcat gggcaataac ctgaaccaga tcgcccggaa agtgaacgcc ggtggtgccg
     2701 gtcatgaccg ggtgcagatc gtggcagcgc tgatggcgat cgatgccggt ctggaacggt
     2761 tgcggcatgc cgtactggaa aaagggccgg acgatgattg ttaaattcca tccccggggt
     2821 cgcggcgggg gcgccggtcc tgtggattat cttctgggta aagaccggca gcgtgaaggt
     2881 gccagcgtcc tgcaggggaa gccggaagaa gtccgggagc tgattgatgc gtcgccgtat
     2941 gtgaaaaaat atacctccgg ggtgctgtca ttcgcggagg cggacctgcc gccgggtcag
     3001 cgtgaaaaac tgatggccag ctttgagcgg gttctgatgc ccggactcga taaagaccag
     3061 tacagcattc tgtgggtgga acacgcagac aagggtcgtc ttgagctgaa ttttctgatc
     3121 cccaataccg aattactgac ggggaaacgc ctccagccgt attacgatcg tgccgaccgg
     3181 ccgcgcatcg atgcgtggca gacagtggtc aacggcaggc tggggctaca tgatccgaac
     3241 gacccggaga accggcgggc gctggtcacg ccttcaggac tgccagaaac gaagcaggaa
     3301 gccgcagaga cgattacacg gggtttgctg atgttagcct catcaggaga gctgaaaacg
     3361 cgtgatgacg tcacaggggc gctcacagcg gccggttttg aggtggtcag aaccacaaaa
     3421 aacagtatca gcattgccga cccggacggg gggcgaaata tccgactgag gggagccatt
     3481 tatgagcagt cttttaacgg cctcgaggga c
//
