LOCUS       NZ_CP125143             4052 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 324 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP125143
VERSION     NZ_CP125143.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790405
            Assembly: GCF_037477835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 43x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477835.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:23:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,808
            CDSs (total)                      :: 5,684
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 54 of 142
            Pseudo Genes (incomplete)         :: 86 of 142
            Pseudo Genes (internal stop)      :: 22 of 142
            Pseudo Genes (multiple problems)  :: 17 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125143.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="324"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(90..548)
                     /locus_tag="P4G14_RS29000"
                     /old_locus_tag="P4G14_28955"
     CDS             complement(90..548)
                     /locus_tag="P4G14_RS29000"
                     /old_locus_tag="P4G14_28955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            1914..2255
                     /locus_tag="P4G14_RS29005"
                     /old_locus_tag="P4G14_28960"
     CDS             1914..2255
                     /locus_tag="P4G14_RS29005"
                     /old_locus_tag="P4G14_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2464..>2895
                     /locus_tag="P4G14_RS29010"
                     /old_locus_tag="P4G14_28965"
                     /pseudo=""
     CDS             2464..>2895
                     /locus_tag="P4G14_RS29010"
                     /old_locus_tag="P4G14_28965"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2933..3418
                     /locus_tag="P4G14_RS29015"
                     /old_locus_tag="P4G14_28970"
     CDS             2933..3418
                     /locus_tag="P4G14_RS29015"
                     /old_locus_tag="P4G14_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_180160425.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNAKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            3422..3634
                     /locus_tag="P4G14_RS29020"
                     /old_locus_tag="P4G14_28975"
     CDS             3422..3634
                     /locus_tag="P4G14_RS29020"
                     /old_locus_tag="P4G14_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1648..1827
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          complement(1185..1665)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          2246..3750
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.024"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtttgttgc cggttttacc tgactgccgc cccgtttgtg acgaacatgt ccggagcggt
       61 gcgggcaaga gatgttatgg taaatcatat tagttctgaa tgattcgggc tccaccagtc
      121 attactattt gagcaccacc tgacattttt atacttgtac cctggctagt cattgtcgta
      181 tcaccttgta tgatcgtttt tccagtagca tgttgacctc caccagtgac taaaattgac
      241 gcttgtactt gagatccatt tatcaaaaca gttccggctt ggtgatcaaa ttttattccg
      301 ctcaatgctt ttaattcagc tccatctagc tgaatttcac cattggctgc tgcagcctga
      361 atggccccct gcatctgaga aaataattta tcaacttcgt caaaaggtaa tggtgtttca
      421 agcagtgagg cttgttgttc aacttcagga tgtttacttg ctaacttccc tatgatgcct
      481 ttgattgcat cagaagtaag attgcttaat gcgccagtga gaacggcaag tagtgtggtt
      541 tcaatcatta tatttccctc gttagattgc tgtgatgcgt attatttact ctttataatc
      601 tccgattctg tccatatggt tagttgcact attacgttta taagtgccac ttttaccata
      661 tatatatcca acgctacatt gtggacattt tggcgtctcg tcattatgag tgaaatttat
      721 ttctcctccg acagataggg tttttacgtt ttccggataa tctaatgtag ccacgccggt
      781 ccattcacaa ttcgagcaga caacatcgaa agatgttggg atactcttgc agttgatgtt
      841 gatttgcata tgcataacct cgattttgca cagaaattac agtgttatta gttgcatacc
      901 gactataacc tgagtttgtg atggagtggt gtttttttgc tttgacttga gtaagagaaa
      961 gagatgatct tcttgagata atctctttct gagcgtagtc ttttgctctg taaacgaaaa
     1021 aaccaccctg gcaggtggtt tttcgaaggt tagttaatcc tggcagattt actaaccgtg
     1081 gtaacagtct tgtgcgagac atgtcaccaa agctgtcctt ccagtgtagc cgcagtaagg
     1141 ccaccacttc aagaactctg aatacatctc tcgcacatcc tgtttaccag tggctactgc
     1201 caatggcgat ttgtcgtgcc ttttcgggtt ggactcaaga cgatagttac cgaaaagagc
     1261 gtggtagtcg gactgaacgg ggggatcgtg catacagtcc agcttggagc gaactgccta
     1321 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac
     1381 cggtaaaccg aatggcagga acaggagagc gcacgaggga gctaccaggg ggaaacgcct
     1441 gggatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag attttatgat
     1501 actcgtcagg ggggcggagc ctatggaaaa acggcaacgc cgcatcctct tcccggcttc
     1561 tcctgatttg agcgctgctt gcgtatcccg tcatctgtga gctggtaacg cccgccgcag
     1621 tctcacggca ggagcgcagc gactgagtga gcgaggaggc gtcatttcac ctttaccagc
     1681 atgttgcact tacgcccgtc agccacattt tccccttccc acatgaagca ctttcataca
     1741 ttaccactcc gccgtaacat atttagccag tatacactcc gctagcgcta cgtgactggt
     1801 tcagggctgc gccccgaaac ccgctaaaag ccactgacgc gcctgcggct tgtccaacac
     1861 cgtgccgacc gggaaacatt tcccgcccgt cccggtgtta tcaggaggct actctggcag
     1921 acaaacgcaa caagatgctc acgatgtggg tgacggaaga cgaacaccgg cggctgctcg
     1981 agcggtgcga cggcaggcag ctggcggcgt ggatgcgtca gtcctgcctc gatgagaagc
     2041 cggcgcgtgc ctgcagactg ccgtcaatat cgccggcgct gctgcgccag cttgccggta
     2101 tgggtaacaa cctgaaccag atcgcccgga aagtgaatgc cgttggtgcc ggtcatgatc
     2161 gggtgcagat tgtggccgcc ctgatggcaa tagacgcggg actggagcgg ctgcggcatg
     2221 cggtgctgga aaaagggggg gatgatgatc gttaaatttc atccccgggg gcgcggtggt
     2281 ggtgccggtc cggtggatta cctgctggga aaagaccggc agcgcgaggg ggcgaccgtg
     2341 ctgcagggaa aaccggagga agtccgggag cttatcgatg cctcgccgta tgcgaaaaag
     2401 tacacctccg gcgtgctgtc gttcgcggag gctgaactgc tgcccgggca gcgggagcag
     2461 atcatggcca gctttgagcg ggttctgatg cccgggctcg ataaagacca gtacagcatt
     2521 ctgtgggtgg agcacaccga caaggggcgg ctggagctga atttcctgat ccccaacact
     2581 gaactgctga ccgggaaacg cctgcagccg tattacgatc gcgccgaccg gccgcgcatc
     2641 gatgcctggc agaccgtggt gaacggcaga ctgggactgc atgatccaaa cgccccggag
     2701 aaccggcggt tactggttac gccgtccgca ctgccggaaa cgaagctgga agccgctcag
     2761 gcgattacac gtgggttgct ggccttagcc tcatccgggg agctcaaaac tcgcgaggac
     2821 gtcacaggtg cgctcacagc ggcaggtttt gaggtggtcc gcactacaaa aaacagtatc
     2881 agcattgccg acccggacgg ggggcgaaac atccgactga agggagccat ttatgagcag
     2941 tcttttagcg ctggcgaagg acttggagca gcaatcgaaa gcgcagcagc agagtaccgg
     3001 cgagatgctg aaagccgcat tcagcgagca cgagaaatct gtaaaagcgg aactgagcgc
     3061 aagcgcgaag agaatcagcg acgccatcag cgcccacgag cagggtatga ccgcggcgat
     3121 gcagtcgaac cgcctgagcg tgctgcgtat ggtcggccgg acgtggctga ccatcaccct
     3181 ggtctcggta ctgctgatcg ccatgagcgg gagcattctg tggtggcagg ggcagcagat
     3241 caccgggaat taccagacta tccgggcgca ggagcggacg caggccatgt tgtcggagaa
     3301 aaaccacggc gttcagctgt cgctctgcgg ggagaagaaa ctgtcctgcg tcaaagtgaa
     3361 cgcgaaagcg ggagcttacg gggaggaagg gaactggatg gtactggaga ggaaatagcc
     3421 catgacggaa ctggaaaaac agttgctgac cgcattagag cagctacatc aggactactc
     3481 gcaaaggctg gacgagtggg agagcgcctt cgcggaatgg cagaagatgt ctggtcttat
     3541 gcaacgggag aacgcggcgc tgaacgagcg cgtcacgcgc ttgagtcagc aggtggcgaa
     3601 cttgagcagg cagttacagc gtttgagcca gtagtgcagc ggcatgaaat ggcggtggcc
     3661 gccgaacggg catacgagca gcgacagcac gaaaaagagc tggcggaagc ccgttcccgt
     3721 cagcggagct ataacggtcc gacgctgggc tgacaggctt caccagtgac aaaccccacg
     3781 aagaagggct cataaatttc ccgctaaata cggtcgtgat gaaaacatca cggcgtatac
     3841 aacggaggga ttatgagact gtttaacgtg attgagacgg tgctgaactg cctgtacatg
     3901 gcgctggaga tctgggactg gctggagaag cattttcctg tttttaaggc gttcatccag
     3961 attctggcct gaaatcagac cgtatgcatc tggctgcatg accatgcagc cgggtaaaat
     4021 ttatctgtca cgaacggggc ggtgtatcgg gt
//
