LOCUS       NZ_CP125142             5010 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 324 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP125142
VERSION     NZ_CP125142.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790405
            Assembly: GCF_037477835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 43x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477835.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:23:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,808
            CDSs (total)                      :: 5,684
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 54 of 142
            Pseudo Genes (incomplete)         :: 86 of 142
            Pseudo Genes (internal stop)      :: 22 of 142
            Pseudo Genes (multiple problems)  :: 17 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125142.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="324"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(621..1022)
                     /locus_tag="P4G14_RS28965"
                     /old_locus_tag="P4G14_28920"
     CDS             complement(621..1022)
                     /locus_tag="P4G14_RS28965"
                     /old_locus_tag="P4G14_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_019725133.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1424..2038
                     /locus_tag="P4G14_RS28970"
                     /old_locus_tag="P4G14_28925"
     CDS             1424..2038
                     /locus_tag="P4G14_RS28970"
                     /old_locus_tag="P4G14_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_019725132.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            complement(2075..2782)
                     /locus_tag="P4G14_RS28975"
                     /old_locus_tag="P4G14_28930"
     CDS             complement(2075..2782)
                     /locus_tag="P4G14_RS28975"
                     /old_locus_tag="P4G14_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004198042.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(2779..3684)
                     /locus_tag="P4G14_RS28980"
                     /old_locus_tag="P4G14_28935"
     CDS             complement(2779..3684)
                     /locus_tag="P4G14_RS28980"
                     /old_locus_tag="P4G14_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_019725130.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replication initiation protein"
     gene            complement(3932..4207)
                     /locus_tag="P4G14_RS28985"
                     /old_locus_tag="P4G14_28940"
     CDS             complement(3932..4207)
                     /locus_tag="P4G14_RS28985"
                     /old_locus_tag="P4G14_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(4188..4433)
                     /locus_tag="P4G14_RS28990"
                     /old_locus_tag="P4G14_28945"
     CDS             complement(4188..4433)
                     /locus_tag="P4G14_RS28990"
                     /old_locus_tag="P4G14_28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            4670..4831
                     /locus_tag="P4G14_RS28995"
                     /old_locus_tag="P4G14_28950"
     CDS             4670..4831
                     /locus_tag="P4G14_RS28995"
                     /old_locus_tag="P4G14_28950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
ORIGIN
        1 tgtttttacg gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt gacaccattc
       61 agccagggga agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca
      121 atttattgcg tggccgtgaa gcgttgccgg cgcagccgga aaaccggttt tcagcacgtt
      181 caggaatcgg ttcgtccacg gccggcaatc acgttatggc aagtcgccgg acgactcacc
      241 acacgattcc ccggcgactc ttcactagag tcgtacccag agtcgccacg tctaaaaaac
      301 aaccaaacgg ggagtcggat ctagagtcgg gtcgttatta actcattgtt tttataggcc
      361 tgagtcgtta ccgactctat gacgactcgc cggggactct atgacgactc gtcatagagt
      421 cggatctata taaataaaat ttaaaaaacg aaatttcagt aaagtaagct gctgatatta
      481 aatacaaatc tgcaaaatta aaattttgcg tggggtgtgg gtatttttcg tggtgaggag
      541 cgttcagaga tctgacgatt ttcgggaagg ttcaggaact ccccggaatg gccggagagg
      601 aaaactgaag catgacgggg tcaggatttt ttccagaatt tccaccacgg cgccgggggt
      661 tcagttaatt ttgcctggtg attttcaaga aggagcatgg cctgtttcag gctgtcaatg
      721 tgcgaatcct tcgataaaat caatatgtta aggtgttcaa tctgctgttt cagttgacga
      781 atgacatgtt cactctggtc agcaggcgct gtatcacgct gttcaggtac ttgtcgagta
      841 aggtgttcat tcttcggttg gtcaaacggt ccgaaaacac gtaaaagctc tgatatatcg
      901 accatttttg tatttcgttc accgctacaa gtggacagtt cacctgtttt gattaacctg
      961 tgcagcgttg tccggctctt acccgtcagc cgtgcagcct cactaataga cactctggtc
     1021 atcagttatc atcctttaat acattcagtc tcaaggaaac aggggccagc ttacgacata
     1081 ctggtccaga agtgatgtgt ttttcagaag ggcgatgaaa cacaggagtg accgtgcaaa
     1141 atgcaggcaa caaatgactt tccgcttcct cgcgcattcg ctcactacgt tcggtcataa
     1201 gactgcggcg agcggtacgt aagaataaag tgaggtgata gctctcttct caaggcgttt
     1261 agggaagcaa cgagcccgcc ctaaaccagt catggcgaga ccttgctgaa gggaccctat
     1321 gtgtgctggc tgagaaaaaa agagctctct aaaagagagc tgttatctga tataggttca
     1381 agtgcaaaat agagtgcaca caaacaaaaa aaggacaata aaaatgggaa agcataaggt
     1441 attcattagc taccaccatg cgaatgatca gtactacaaa aacgcacttg aaaaaatgaa
     1501 tgaggaacat gaaatattcg tgaatcgctc agtatcatta ggtgatatag atgaagatga
     1561 agccccacaa aaaataagag agataataag agatgaatat cttagagata catcagttct
     1621 tatccttctt gttggaactg aaacaaaaaa cagaaaacac gtagattggg aactttactc
     1681 tagcatgaga gatagtacca ttaacaaaaa atcaggaatt ttagtcgtca atctcccgtc
     1741 aacaaatact acgtacattc gttcaacaca tggaactaat gaaaaatcag aagttcatcc
     1801 taccgtaact gaatggtttt ctattgataa gcgctcaact tatgaggaaa ggttcccata
     1861 tatgccagca agaataatag acaacttcat gaagagcggg tcttatatat cagtggtcaa
     1921 ctggaatcaa atagaaaaca acccagaaac actcagaaaa ttaatagaca tgacttataa
     1981 cgatagagaa aaagcaacgt atgatatgtc gagagaaatg agaatgcata attcatagta
     2041 ctatccaagg agtagccttg gctactcctt taatttacca gttccactta tctgtattga
     2101 ttccaaactt aggcttcaca tcattaacaa agaaccggtt aacatcttta aaacctttta
     2161 aaatacgctc ataatcataa tactgcattt gtattttatt tatgctggtt gcatgaaatc
     2221 tcatgacatc accgctaacc ctatcaacaa cctcatcttt gtctgatatg tacttatacc
     2281 aacgcttgta gttgttctgg tagttagtta gaaatctatt taacaccatg agcatctcat
     2341 tgctgaaatc ataaatctca cgatcttttt tagggtttag tattttcatt tcatcacgaa
     2401 taaattggta agtctgataa tatgatgaaa taacctcatc aaaggcatca acgttttttc
     2461 ttaatttgaa aattgctctt tttgaatcta ggtgattttt tacactcttt ttgtaaagtg
     2521 tagcactctc attgaaaagt atattcactc caccaaggct aagtttctca accttaaccg
     2581 aatacttttt aagtaagtag agggatacca tcagccaaat aaaaacagat atcaaaagaa
     2641 tagaaagggc gccagttaca agaggtctat tcttactaaa aactgtgtaa acagacggaa
     2701 tgtaattaga tatatcaaca gtacacatca cggcaataaa tgctaaaaaa actatagtcc
     2761 aaagagttac gaaggttttc atcttatttc accatttttc ttgatataaa aatacttccg
     2821 cctgcttatt cccatagcta cccaaggttt tatttcctct aggcttcctt tcctccttga
     2881 ctgtcctgac tttttccctc tctttgattg aatttcagag gaatgagtaa tatcaacaaa
     2941 tttactgaat tcttcaggtg aaaatttatt tgatgtccat ttagcaatgc tcttagctat
     3001 ccctcttacc tcattttcat ccagaggccg tgcaaattgc aggttgtatg cgcaagcgcg
     3061 ttcgtagcag gcttgcagcc actgctcata ctcaggccag ccctgacgga tggcgcgata
     3121 tgcccactta cgggttttgt cgaacaacgt gcagttacgg cctaagccgt agtcggcaac
     3181 gatttccttg tcattccctg tgttcagatc gagaaagtcg gccagccagt ccagagtgta
     3241 gagcttcggc tgccacacgg cgattttcca gtggttgtgg ttcggatttt tgcagatgag
     3301 ccccgaatat ccagcgtcag cctctaattt tttacgtagc gcattctcca cggaggcggc
     3361 gtagcgtaac ggttttattt tgccgtccgg cgcggtgcgt atcgagtttt ccagcgcgta
     3421 cagcaggtgc gcgtgtccgt tttcggggtt agttatggtc agcgtcggtg caggcgcgtt
     3481 gcggtcgctc cagtcgatgg aggaaccggc gcggtcaacg tcaaatccca gccagaacat
     3541 ggcgtgaggc tgattgaact ggatatattt cgccagaata gcgcgttcct taccggcgat
     3601 acggacacca aagtgcagat cgtccgaaaa gtacggcttg tgtggtaagc gttcgttgaa
     3661 aagttcgagt gcggcgttat gcaatgtgcc ctgcgcttat ggcgggctgt tgtctggcac
     3721 ggttcatgtg ctaatatcct ttcaaggttt cgacctagcc ctggttatca tgcgatccgc
     3781 caagatatga catgatttcc ggggctttgt tttttctgag gctaacgtta caccgacttt
     3841 gcctttgtgt cgatctgaca gcgacttacc ctaacatccg ttgtacgcct tgcatgaaaa
     3901 gcccgcatca agcgggctat atcgactttt gctatttgtc accatagtga taacgggctg
     3961 cgtagatgat gatttctccg ttctggattg aatagaccag acggtggctc tgatcaatcc
     4021 tgcgggagta gagtgccgga tctttgtggt gccgcagccg ttcgggtttt cctatacctg
     4081 tcagcggatc agcctcgatg ctgagcaaca gttgccggat acgctcaaac ttctttttgt
     4141 cagtatcacg ccagtagtta aggtcatcag cgccattcct agtgaactta atattccact
     4201 tcgacaaatt cgccgcgctc cgcctgtttc attgactcat tgatatgctc tgcgttcgcc
     4261 ggattgctga acagatagac cgtttcgatt agcgcctcat actctcccgc atcgatcata
     4321 acaatatcag gcgcatcacg gcgcatgaca cgtaccggtt cagcatcatc agtgacgcgc
     4381 ttcatcgtct cggcaaaatg tttacgggca tcagtaaaat tgattgcatg catgttgtta
     4441 acccccattt gttggggctt agcatagaac atgtacgcaa ataagtacat aaagatattt
     4501 ccctaaaaac ccacaaccaa atcagctcaa aatgtgaact caatcgaata accacaaaaa
     4561 caacaagtca atacattgaa gcataatttc aagcaattac atttatgaag tgcggcacaa
     4621 tttcgtaccg ctaggagctt agctcctaaa acgagcaaag gaaaagtaaa tggcaaaaca
     4681 ttggtctggg caagaatgtc caaaaacagg tacttacggt caatatcatg ataccgacaa
     4741 ctcatacgct ggttctgaat atgatcgcca tgtgagcaag ggtgataagt tccctccgag
     4801 caaaaacaac caccacttcc aagaaaaata aaatatcttc agaagtctaa actaaaagcc
     4861 ctctggggct ttttttgtac ctgccattca cttgatttac cataacatcc gttatcagca
     4921 ccgcctgaga tgccctcagg gcgtttctga cgggaaaccg gtaaacaagg caacaaacca
     4981 cccgaaaacc cgccagaaac gcgcaggctc
//
