LOCUS       NZ_CP125141             9294 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 324 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP125141
VERSION     NZ_CP125141.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790405
            Assembly: GCF_037477835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 43x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477835.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:23:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,808
            CDSs (total)                      :: 5,684
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 54 of 142
            Pseudo Genes (incomplete)         :: 86 of 142
            Pseudo Genes (internal stop)      :: 22 of 142
            Pseudo Genes (multiple problems)  :: 17 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125141.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="324"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            81..230
                     /locus_tag="P4G14_RS28910"
                     /old_locus_tag="P4G14_28865"
     CDS             81..230
                     /locus_tag="P4G14_RS28910"
                     /old_locus_tag="P4G14_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1711..3675
                     /locus_tag="P4G14_RS28915"
                     /old_locus_tag="P4G14_28870"
     CDS             1711..3675
                     /locus_tag="P4G14_RS28915"
                     /old_locus_tag="P4G14_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            3675..4406
                     /locus_tag="P4G14_RS28920"
                     /old_locus_tag="P4G14_28875"
     CDS             3675..4406
                     /locus_tag="P4G14_RS28920"
                     /old_locus_tag="P4G14_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            4413..4943
                     /locus_tag="P4G14_RS28925"
                     /old_locus_tag="P4G14_28880"
     CDS             4413..4943
                     /locus_tag="P4G14_RS28925"
                     /old_locus_tag="P4G14_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4971..5150)
                     /locus_tag="P4G14_RS28930"
                     /old_locus_tag="P4G14_28885"
     CDS             complement(4971..5150)
                     /locus_tag="P4G14_RS28930"
                     /old_locus_tag="P4G14_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5219..5596
                     /locus_tag="P4G14_RS28935"
                     /old_locus_tag="P4G14_28890"
     CDS             5219..5596
                     /locus_tag="P4G14_RS28935"
                     /old_locus_tag="P4G14_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(5658..5957)
                     /locus_tag="P4G14_RS28940"
                     /old_locus_tag="P4G14_28895"
     CDS             complement(5658..5957)
                     /locus_tag="P4G14_RS28940"
                     /old_locus_tag="P4G14_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5947..6210)
                     /locus_tag="P4G14_RS28945"
                     /old_locus_tag="P4G14_28900"
     CDS             complement(5947..6210)
                     /locus_tag="P4G14_RS28945"
                     /old_locus_tag="P4G14_28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(6516..6935)
                     /locus_tag="P4G14_RS28950"
                     /old_locus_tag="P4G14_28905"
     CDS             complement(6516..6935)
                     /locus_tag="P4G14_RS28950"
                     /old_locus_tag="P4G14_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7338..9023
                     /locus_tag="P4G14_RS28955"
                     /old_locus_tag="P4G14_28910"
     CDS             7338..9023
                     /locus_tag="P4G14_RS28955"
                     /old_locus_tag="P4G14_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9033..9290
                     /locus_tag="P4G14_RS28960"
                     /old_locus_tag="P4G14_28915"
     CDS             9033..9290
                     /locus_tag="P4G14_RS28960"
                     /old_locus_tag="P4G14_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     BGC             2338..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(334..912)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3676..4403
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 aagttatcaa ggatgatttg aaatacagac ttccggtgtt cagggatgaa taccggggtt
       61 tttaaataca gggaaggtat atgaaaaagg taaaggttgt ttttcttttt atattgattt
      121 tttcaggctt tcttctggtc gcatgtcagg caaactatat ccgggatgtt cagggtggaa
      181 cggtggcacc atcgtcctcc tctgaactga cggggatcgc ggttcagtag aaaagatcaa
      241 aggatcttct tgagatcctt tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac
      301 cacctgggga ggtggtttga tcgaaggtta agtcagttgg ggaactgctt aaccgtggta
      361 actggctttc gcagagcaca gcaaccaaat ctgtccttcc agtgtagccg gactttggcg
      421 cacacttcaa gagcaaccgc gtgtttagct aaacaaatcc tctgcgaact cccagttacc
      481 aatggctgct gccagtggcg ttttgcgtgc ttttccgggt tggactcaag tgaacagtta
      541 ccggataagg cgcagcagtc gggctgaacg gggggttctt gcttacagcc cagcttggag
      601 cgaacgacct acaccgagcc gagataccag agtgtgagct atgagaaagc gccacacttc
      661 ccgcaaggga gaaaggcgga acaggtatcc gggaaacggc agggttggaa caggagagcg
      721 caagagggag cgattcatcg gaaacggtga tgatctttaa gtcctgtcgg gtttcgccac
      781 tcctgactga ttcatggttg agccaccggc tcccacagat gcaccgaaaa agcgtctgtt
      841 tatgtgaact cagtcaggag ggcggagcct atggaaaaac gccacctgcg tggccttgcc
      901 gttttgcctc acatgttagt cccctgctta ttcacggaat ctgtgggtaa ctttgcatgt
      961 gtccgcagcg cccgccgcag tctcacggcc ggagcgtagc gaccgagtga gcgaggaggc
     1021 gcaatttccc cggcacctac aagacggaca ccggacgcgc tgatgtggca tattgtcccc
     1081 tgattaagtc tttatattcc ctgataataa attaccagga taggtgaatc atatcggttt
     1141 ttcgctgaac attttacgtt aacctatctc tgttgctttt ttctccttac ggagaaatac
     1201 aggtcatccc cgcgagggcg tggctgacct cagggggata tcccctgctt acgctggctg
     1261 gacgccaccg acgcacggtc tctccccctc tttctggctc cgtccggctg gacgccggcc
     1321 acgccagaaa ttcacccggc ccccgtcagg ctcacgcctg accacccaca aaaatccacg
     1381 gtgctggacg cacctggcga agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc
     1441 tcgctatggc gaactctgac gggggccgga acaccggaaa agaccgcact ggggtgccgc
     1501 cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca
     1561 tggggcggaa agaggctggc ctccgtggtg aaagctgaac gcaatcacca cggacgtgac
     1621 cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca gcgggggctg cacatcacgc
     1681 cggcaaacca tcgtctgaag gagagagaac atgtttaata cggattcgct tgcctggaag
     1741 tggcacattc cggagctggc ccggcagatt tatccgtggg tcagctggct gtaccaggag
     1801 tattacggtc tggccggggc tttcctagtt atcggcctgt ttttcggtct ggctgaactt
     1861 tgggccggac ggatgcgttt tgtggggctg agtcataagg agcgacggga gtatgccgat
     1921 cgctataagc gcgataataa acggccagcg ccattttcgg tgctggcctc gctggattcg
     1981 cccgggcgga tggtcagggc acgtcgacat gtctggaact acgcgtactg gctgaccggc
     2041 gcggccggtc tggcccttct gggggggctg gttgtcgccg tgtttatcga gagctgggga
     2101 gaaaccgtcc tgcgtccgga cctctacgca aacgcagatt attacctgct gcagccggct
     2161 aacctgttcg ggattgcctg gtgcgcgggc gggctggctg tcgggattgt gttggcgcgg
     2221 ctgctggcgt tcctgctgct tgacggtcat tttgcggctg ctgacgaggc ggtgaacgcg
     2281 aaactgaatc aggagagccg gcgcagcagc cagcggacag gagagatgac cgacgtgcgc
     2341 cacctgcatt tcggggagcc ggtaccggtt aatgcgctgg ccgatttttc ctcggagcag
     2401 gcccggcagg agcaggcggt gtttctgggt aaggatgagc agggcaagcc ggtgctggtt
     2461 ccgcgtgata cctggcgcaa gaccaacatt cagatcctcg gcctgcccgg gagcggcaag
     2521 agcgtgatgg ccaccaacgc gctcattcgc tgcgtacgcg atttcgggga cgcggtggtg
     2581 tacttcgacc ccaaagggga cgcatgggcg ccgcacgtct tccgggcgca ctgtccggac
     2641 ttcaccctgc tggatctgcg gccggggaaa ccggcgcagc tgaatctgtt ccgcgatctc
     2701 gaccagtacg cgctgaaaaa cctgctgatt gccgggctta acctgggcga gaccggcgac
     2761 gtggcggacc actaccgcat cagcgagcag aaagcggcga agctgattgc ggagcagttt
     2821 ccgcaggggg caaacattca gcaggtgctg gcggcggcgt acgcgctgcc ggaggccctg
     2881 aaaaaggacg tgaaggggtt gatcactagg ctggaaaacg tcgctgacct gagcgtcctg
     2941 cagacggaca gtggcatcga tgtggcgggg attatcaacg gcggcggctg cctgtacgtt
     3001 atcggctcga tggacgacga ggcggtgatc cgtgtgcaaa aaatgctgtt tgcccgctgc
     3061 gcgcaaatca tcattgcgcg ggacgagttc cgggagtggc cacacgtcag cgtgatgctc
     3121 gatgaaatta aatatctgct gtcgaagtat gtactgaacg cactggggac gctgcgcagc
     3181 cgggactgca atctgttgct cgcgcaccag tcgctgggcg atttcgggca gtgcgggcag
     3241 gatctgcccg ctgaatttgt gaaaaccacg gtactggata acacgccgat ccgctggttt
     3301 taccgggcgg ccagcctgga gtccgcgcag tgggcggcgg ggcagaccgg ggaaatcagg
     3361 gtggacgtgg agcgccgccg tgccagccgg gaggcgggga acgtggagca tatcagcggg
     3421 gacagcttca tccagaagga ggcgcggccg ctgtttgacg tgaacaccct gcaacacctg
     3481 ccggacgggt tcgcggtcat gaccgggctg ggtgtggccc ggatggcgtt cagcagtccg
     3541 ctgcgggttg agcgccggga aatcccgttg cagcgtttcc cggtgctggc gaaaaccgat
     3601 cccctggcgg agttccggcc cccggccgac ggcggcgggc cgaaagcgga tgatgatttt
     3661 gcggggattt actgatggcg ctggagctgt acaacgtcag ccacgcgaaa cgacaggcgc
     3721 ggaacgcgga gaaaacccgg ctgaccctgc gctggttgcg ggaagagctg tgttccacgg
     3781 cggagctggt tgcgcggcgg ctggggattg cggcggtgca gccggtctac cgcttcctcg
     3841 acagcctggt ggcgaaaggg ctgctggtcc ggaccaaata tccggtggac ggccgtcagg
     3901 tcagcgtgtg ggggctgacg ccgcatggcg tggcgttctc gttcgacgag gacgaaccgc
     3961 tggcggatgt tattccgttt cagccgtcaa gggtgtcggc agcacatctg ccgcaccggc
     4021 tggccgtgca atccctgcgc cttgatatgg aagccagagg ggccaccggc tggcgcattc
     4081 tgcaccgtct gagtcttaaa ggcatgaaag tgccggacgc cctggcggag ctggacggta
     4141 aaacggtggc gtttgaggtg gagcgcaccg tcaagagccg tcgccggtat caggaagtgg
     4201 tgtccgggta tctgttcaac cgcaaagcta acggcatcga tgaaatctgg tacatctgcc
     4261 cggaccgggc gacgcaggtc agggtgcagc gggcaatcct gtcggtggat gaaatcgtga
     4321 acccacagac cggcgaggcc cgcaaaacgg cagagctgga tcgtgaacgg ttgtttgcgt
     4381 gctttaaatt tatgacaacg gagtgaggga ttatggcgct gtacatggta tttattgaag
     4441 acgcggagga ccggaacggc gagcgctgca tcatgccgac cagcgagcat ctggaagata
     4501 tggtggtgct cgcctggcag accggggcgt atgttttctg ttttgtcgac cgggcccagc
     4561 atctggcgat gatgaaatcc atcgggatgt atgacgagcg gctcaacagc gacgaaaaag
     4621 ccacgtactt tttcactggt aaaaatccgc aatgggaacg gaacaaaccc acaggaatga
     4681 agctgtgtga ttacctctat cgcaaggtgg agcggttcgc catcgagaac ggtgtgcagg
     4741 tggttgatgc cgggaagtgc tcaccggagc agattaacgc gatgattgac gtggtgagtg
     4801 agcggcacgg ggaaagcgcg gtgaacgaga agctgaaagc ggagctgatg cgggaatata
     4861 acagccgcta ttttaacgtg ccggtacctg aactgccgga ggacaaacct gcccgccggt
     4921 ggtggccgtt cggcaaaaaa taaatgccag caccggccat gccggtgctg ttattcctcc
     4981 ccgagcttat cccgcatcac ctgccagagt gtttccgcat cctcgtgtag ccgctcgcac
     5041 atgtcggccg gggtatccat atccagctca ttcagtttat cgagcaatag cagcgactgc
     5101 tccttaataa atttagccat attgagggct gttttttcct gttttgtcat gattctttcc
     5161 ttccgggcat ggtgtgcgcc tgtcaggtat actgtctcaa aaatagcggg gtgagacaat
     5221 ggcgtgggtg acggtgagac aggcgacgga actgacgggt aaagcccgaa gttccctgta
     5281 tcgggatatg gcgaaaggcc gcgtatcata ccgtacggaa gctgatggcg gccgggtggt
     5341 ggataccagc gagcttatcc gggtgtacgg tgagttaaga caggatgaga ctcacgagcg
     5401 ggacgagttg agactgtcgg gtgagactga aaaaatggcc tccgacgcgt tgatcgcgga
     5461 gataaaagca ctcagggagg aggtggccgg gctacggcag gaaatgcagt ccatgagact
     5521 gctggaacac aaaccggaga cagcagagac cccggccgga acaccgcgcc ggtggtggcc
     5581 gtggggaaaa cgctgacgcg ctgtgcttgt ccaaccggcg acggccacgc aatgaattgc
     5641 gtccccgtca ccggttatta gagaacaata tggggattct ggcgctggcc gaccacggcc
     5701 agaacccgga cttttctggt ctggtcatca acatcataga ggacatgctg tcccatcaga
     5761 ctgatccggc gaacgtcctc gccatagcgt gagtccactt catagagtcg cgggaagtcg
     5821 gccagtttgt ccgccgcagc gagaacccgg tcggtaacgt tctgcgcatc ccacagggat
     5881 gaattttccg cgatatagtc aaacagcgtc gttacatcct cgatggctcc ctgcttccat
     5941 tccacgtcat acggcatcgc gcagttcctg ataacggcca tgcaggcgct ggcggagttg
     6001 ttccgtggtc atacccggac gcgtgtcagc acgggcggcg gtcactttgg cgcgaacctg
     6061 atcttcatac tctgcctggg ccagttcttc gttagccagt cgctgcaaca gcgcagcttt
     6121 gtccgggcca ccttccggca gtatgcgggg caggctttca gccagttccc gaagaaagtt
     6181 ggcgttttcg tagtgtgtag tgttgctcat tgcggagcct cccgggttaa tgcggcaata
     6241 tgaaggaagt ttagcagggg gcggtctgaa agaatatggt cgtgtcagtc aggctgatga
     6301 agcagaaagt cggtaaaaaa gggcttacgg catccatttt acgtcaaaac atatgccatg
     6361 cagttgcgtg cataggcatg cattaggata aaatttatcg ggcgcgtttc cggcagtttt
     6421 ccgggggggt tgttgcctgt tttatcccgt agccgccgga aacgccctga gcccgtctga
     6481 gcggtgcgcg taatgacgcg ttatggtaaa tccacttact gtctgccttc atagccatcg
     6541 agataaacag ctgaaatggt gtcagccagt agccttgatt ttttcggtgt gaggtctttg
     6601 ctgcggtaat cggtacccgc aaattgtgtt gtgccttgga gtgagtttaa aaattcctga
     6661 ctgtgaaact gctgaatgcg ttgctgcaca tcactccgtg attttccggt cgctttatcc
     6721 tttgcgccca tatcccagaa ctgccggaaa aaaggcacca tcattcgtgt tgtgtcagct
     6781 ttctgggtgg aaaaattggg atcaaaaccg tcatctgaag catatacgta atgccgggta
     6841 tgctgttcca cgctgccctt tggagcacag gcagtgagag ttgctgtcgc tgttatcatg
     6901 gcgataagac acagtttgct gccggaacgt ctcatgagcg tctccttacc tttaattctt
     6961 ttttctcatt gagttatata tccgcaggct atcttatcat cagtttctga ccgtgttcag
     7021 tgaccgaaca acccgaacca gaccccccgt ttcttttcct gtcccggagg gggctttctg
     7081 gtatctcata taatgatttc agtaaaacat actgaggccg gagaacggcg ccgtttgtgc
     7141 tggtttttgt tgcatctgga ggggattgcg catggtgtga gtggctgttt ttttgatcat
     7201 tttcacaaaa acgcaaattt acagacttga catgaaaaac aaggcagcgt aatttttata
     7261 ctgtatataa aaccagtggt tatatgtaca gtattttatt ttagtttatt gttttaaaag
     7321 tcaaagagga aaagtatatg agtggcggag acggacgagg tccgggtaat tcaggtctgg
     7381 gacacaatgg tggtcaggcc agtgggaatg tgaacggtac atcaggtaaa ggtggccctt
     7441 catcaggtgg tggtacggat ccaaacagcg ggccgggctg gggtacgacg catacaccta
     7501 acggggatat tcataactat aacccggggg agtttggtaa cggcgggagt aaacccggtg
     7561 gtaatggcgg taacagcggc aatcatagcg ggagctctgg tggcggacag tcttcggcca
     7621 ccgcgatggc cttcggtctg cctgccctgg ccactccggg ggctgaagga ctggctttat
     7681 ccgtttccgg cgatgcgttg tcggccgctg ttgctgatgt gctggctgcc ctgaaaggtc
     7741 cgtttaagtt tggtctgtgg gggattgcga tttacggagt gctgccttct gagatagcaa
     7801 aagatgatcc gaaaatgatg tcaaaaatcg tgacgtcatt gccggcagac acggtaacgg
     7861 agacgccggt cagctccctg ccgctggacc aggcgacggt cagcgtcact aaacgtgtgg
     7921 cggatattgt gaaggacgag cggcagcata ttgcggttgt caccggccgg ccaatgagtg
     7981 ttcctgtggt ggatgcgaaa ccgacaaaac gtccgggggt attcagtgtg tcgattccgg
     8041 gtctcccgtc tctgcaggtg agcgtaccta aaggtgttcc ggccgcgaaa gccccgccaa
     8101 aaggcattat tgctgaaaaa ggtgattcac gtccggctgg ttttacggcc ggtggtaact
     8161 cccgtgaggc cgttattcgt ttcccgaaag agagcggaca gaaaccggtc tatgtgtcgg
     8221 tgacggatgt tcttactccg gcacaggtaa aacagcgtct ggaggaagaa aagcgtcgcc
     8281 agcaggcatg ggacgctgct cacccggaag aggggctgaa aagagagtat gataaagcga
     8341 aagctgagct ggatgctgaa gataaaaata ttgcgacctt aaacagtcgc attgcatcga
     8401 cagagaaggc gatccccggt gcaagggctg ctgttcagga agccgataaa aaggtaaaag
     8461 aggcagaggc aaataaagat gattttgtga cttataaccc gcctcatgaa tatggctccg
     8521 ggtggcagga tcaggttcgc tatcttgata aggatattca gaatcagaat gagaaattaa
     8581 aagctgccca gacatcttta aacgaaatga atgaatcctt atccagggat aaggctgcgc
     8641 tttccggggc gatggagagc cggaaacaaa aggagaaaaa agcgaaggat gcagaaaata
     8701 aattaaatga ggaaaagaaa aaacctcgta agggaactaa agattacggc catgattatt
     8761 ttcctgatcc caagactgaa gatattaagg ggttgggaga gttgaaagag ggtaaaccta
     8821 aaacccctaa acaaggtggt ggtggtaagc gggcccgatg gtatggtgat aaaaagcgta
     8881 aaatttatga atgggattcc cagcacggtg agcttgaagg gtaccgcgcc agtgatggcg
     8941 aacacctcgg ggcattcgat ccaaaaacgg gtaagcaggt taaagggccg gatccaaaac
     9001 gaaacattaa aaaatatctt taagaggtaa atatggggct taaattacat attcattggt
     9061 ttgataagaa aaccgaagag tttaaaggtg gtgaatactc aaaagacttc ggtgatgatg
     9121 gttctgtcat tgaaagtctg gggatgcctt taaaggataa tattaataat ggttggtttg
     9181 atgttgaaaa atcatgggtt tcgatattac agccacactt taaaaatgta atcgatatta
     9241 gtaaatttga ttactttgta tcctttgttt accgggatgg taactggtaa gcaa
//
