LOCUS       NZ_CP125136             4052 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 331 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP125136
VERSION     NZ_CP125136.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790407
            Assembly: GCF_037477825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 131x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477825.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:33:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,705
            CDSs (total)                      :: 5,580
            Genes (coding)                    :: 5,441
            CDSs (with protein)               :: 5,441
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 58 of 139
            Pseudo Genes (incomplete)         :: 82 of 139
            Pseudo Genes (internal stop)      :: 21 of 139
            Pseudo Genes (multiple problems)  :: 19 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125136.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="331"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            964..1422
                     /locus_tag="P4G30_RS28485"
                     /old_locus_tag="P4G30_28445"
     CDS             964..1422
                     /locus_tag="P4G30_RS28485"
                     /old_locus_tag="P4G30_28445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            complement(1930..2142)
                     /locus_tag="P4G30_RS28490"
                     /old_locus_tag="P4G30_28450"
     CDS             complement(1930..2142)
                     /locus_tag="P4G30_RS28490"
                     /old_locus_tag="P4G30_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2146..2631)
                     /locus_tag="P4G30_RS28495"
                     /old_locus_tag="P4G30_28455"
     CDS             complement(2146..2631)
                     /locus_tag="P4G30_RS28495"
                     /old_locus_tag="P4G30_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_180160425.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNAKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2669..3346)
                     /locus_tag="P4G30_RS28500"
                     /pseudo=""
     CDS             complement(<2669..3346)
                     /locus_tag="P4G30_RS28500"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3309..3650)
                     /locus_tag="P4G30_RS28505"
                     /old_locus_tag="P4G30_28465"
     CDS             complement(3309..3650)
                     /locus_tag="P4G30_RS28505"
                     /old_locus_tag="P4G30_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3738..3917)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          38..546
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.991"
                     /coverage="99.0"
                     /evalue="1.74e-142"
     Typing          complement(1815..3319)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.024"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccctgacgag tatcataaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
       61 ataaagatcc caggcgtttc cccctggtag ctccctcgtg cgctctcctg ttcctgccat
      121 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
      181 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgatccc cccgttcagt
      241 ccgactacca cgctcttttc ggtaactatc gtcttgagtc caacccgaaa aggcacgaca
      301 aatcgccatt ggcagtagcc actggtaaac aggatgtgcg agagatgtat tcagagttct
      361 tgaagtggtg gccttactgc ggctacactg gaaggacagc tttggtgaca tgtctcgcac
      421 aagactgtta ccacggttag taaatctgcc aggattaact aaccttcgaa aaaccacctg
      481 ccagggtggt tttttcgttt acagagcaaa agactacgct cagaaagaga ttatctcaag
      541 aagatcatct ctttctctta ctcaagtcaa agcaaaaaaa caccactcca tcacaaactc
      601 aggttatagt cggtatgcaa ctaataacac tgtaatttct gtgcaaaatc gaggttatgc
      661 atatgcaaat caacatcaac tgcaagagta tcccaacatc tttcgatgtt gtctgctcga
      721 attgtgaatg gaccggcgtg gctacattag attatccgga aaacgtaaaa accctatctg
      781 tcggaggaga aataaatttc actcataatg acgagacgcc aaaatgtcca caatgtagcg
      841 ttggatatat atatggtaaa agtggcactt ataaacgtaa tagtgcaact aaccatatgg
      901 acagaatcgg agattataaa gagtaaataa tacgcatcac agcaatctaa cgagggaaat
      961 ataatgattg aaaccacact acttgccgtt ctcactggcg cattaagcaa tcttacttct
     1021 gatgcaatca aaggcatcat agggaagtta gcaagtaaac atcctgaagt tgaacaacaa
     1081 gcctcactgc ttgaaacacc attacctttt gacgaagttg ataaattatt ttctcagatg
     1141 cagggggcca ttcaggctgc agcagccaat ggtgaaattc agctagatgg agctgaatta
     1201 aaagcattga gcggaataaa atttgatcac caagccggaa ctgttttgat aaatggatct
     1261 caagtacaag cgtcaatttt agtcactggt ggaggtcaac atgctactgg aaaaacgatc
     1321 atacaaggtg atacgacaat gactagccag ggtacaagta taaaaatgtc aggtggtgct
     1381 caaatagtaa tgactggtgg agcccgaatc attcagaact aatatgattt accataacat
     1441 ctcttgcccg caccgctccg gacatgttcg tcacaaacgg ggcggcagtc aggtaaaacc
     1501 ggcaacaaac cacccgatac accgccccgt tcgtgacaga taaattttac ccggctgcat
     1561 ggtcatgcag ccagatgcat acggtctgat ttcaggccag aatctggatg aacgccttaa
     1621 aaacaggaaa atgcttctcc agccagtccc agatctccag cgccatgtac aggcagttca
     1681 gcaccgtctc aatcacgtta aacagtctca taatccctcc gttgtatacg ccgtgatgtt
     1741 ttcatcacga ccgtatttag cgggaaattt atgagccctt cttcgtgggg tttgtcactg
     1801 gtgaagcctg tcagcccagc gtcggaccgt tatagctccg ctgacgggaa cgggcttccg
     1861 ccagctcttt ttcgtgctgt cgctgctcgt atgcccgttc ggcggccacc gccatttcat
     1921 gccgctgcac tactggctca aacgctgtaa ctgcctgctc aagttcgcca cctgctgact
     1981 caagcgcgtg acgcgctcgt tcagcgccgc gttctcccgt tgcataagac cagacatctt
     2041 ctgccattcc gcgaaggcgc tctcccactc gtccagcctt tgcgagtagt cctgatgtag
     2101 ctgctctaat gcggtcagca actgtttttc cagttccgtc atgggctatt tcctctccag
     2161 taccatccag ttcccttcct ccccgtaagc tcccgctttc gcgttcactt tgacgcagga
     2221 cagtttcttc tccccgcaga gcgacagctg aacgccgtgg tttttctccg acaacatggc
     2281 ctgcgtccgc tcctgcgccc ggatagtctg gtaattcccg gtgatctgct gcccctgcca
     2341 ccacagaatg ctcccgctca tggcgatcag cagtaccgag accagggtga tggtcagcca
     2401 cgtccggccg accatacgca gcacgctcag gcggttcgac tgcatcgccg cggtcatacc
     2461 ctgctcgtgg gcgctgatgg cgtcgctgat tctcttcgcg cttgcgctca gttccgcttt
     2521 tacagatttc tcgtgctcgc tgaatgcggc tttcagcatc tcgccggtac tctgctgctg
     2581 cgctttcgat tgctgctcca agtccttcgc cagcgctaaa agactgctca taaatggctc
     2641 ccttcagtcg gatgtttcgc cccccgtccg ggtcggcaat gctgatactg ttttttgtag
     2701 tgcggaccac ctcaaaacct gccgctgtga gcgcacctgt gacgtcctcg cgagttttga
     2761 gctccccgga tgaggctaag gccagcaacc cacgtgtaat cgcctgagcg gcttccagct
     2821 tcgtttccgg cagtgcggac ggcgtaacca gtaaccgccg gttctccggg gcgtttggat
     2881 catgcagtcc cagtctgccg ttcaccacgg tctgccaggc atcgatgcgc ggccggtcgg
     2941 cgcgatcgta atacggctgc aggcgtttcc cggtcagcag ttcagtgttg gggatcagga
     3001 aattcagctc cagccgcccc ttgtcggtgt gctccaccca cagaatgctg tactggtctt
     3061 tatcgagccc gggcatcaga acccgctcaa agctggccat gatctgctcc cgctgcccgg
     3121 gcagcagttc agcctccgcg aacgacagca cgccggaggt gtactttttc gcatacggcg
     3181 aggcatcgat aagctcccgg acttcctccg gttttccctg cagcacggtc gccccctcgc
     3241 gctgccggtc ttttcccagc aggtaatcca ccggaccggc accaccaccg cgcccccggg
     3301 gatgaaattt aacgatcatc atccccccct ttttccagca ccgcatgccg cagccgctcc
     3361 agtcccgcgt ctattgccat cagggcggcc acaatctgca cccgatcatg accggcacca
     3421 acggcattca ctttccgggc gatctggttc aggttgttac ccataccggc aagctggcgc
     3481 agcagcgccg gcgatattga cggcagtctg caggcacgcg ccggcttctc atcgaggcag
     3541 gactgacgca tccacgccgc cagctgcctg ccgtcgcacc gctcgagcag ccgccggtgt
     3601 tcgtcttccg tcacccacat cgtgagcatc ttgttgcgtt tgtctgccag agtagcctcc
     3661 tgataacacc gggacgggcg ggaaatgttt cccggtcggc acggtgttgg acaagccgca
     3721 ggcgcgtcag tggcttttag cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct
     3781 agcggagtgt atactggcta aatatgttac ggcggagtgg taatgtatga aagtgcttca
     3841 tgtgggaagg ggaaaatgtg gctgacgggc gtaagtgcaa catgctggta aaggtgaaat
     3901 gacgcctcct cgctcactca gtcgctgcgc tcctgccgtg agactgcggc gggcgttacc
     3961 agctcacaga tgacgggata cgcaagcagc gctcaaatca ggagaagccg ggaagaggat
     4021 gcggcgttgc cgtttttcca taggctccgc cc
//
