LOCUS       NZ_CP125135             5010 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 331 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP125135
VERSION     NZ_CP125135.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790407
            Assembly: GCF_037477825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 131x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477825.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:33:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,705
            CDSs (total)                      :: 5,580
            Genes (coding)                    :: 5,441
            CDSs (with protein)               :: 5,441
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 58 of 139
            Pseudo Genes (incomplete)         :: 82 of 139
            Pseudo Genes (internal stop)      :: 21 of 139
            Pseudo Genes (multiple problems)  :: 19 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125135.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="331"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(join(4432..5010,1..129))
                     /locus_tag="P4G30_RS28450"
                     /old_locus_tag="P4G30_28410"
     CDS             complement(join(4432..5010,1..129))
                     /locus_tag="P4G30_RS28450"
                     /old_locus_tag="P4G30_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004198042.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(126..1031)
                     /locus_tag="P4G30_RS28455"
                     /old_locus_tag="P4G30_28415"
     CDS             complement(126..1031)
                     /locus_tag="P4G30_RS28455"
                     /old_locus_tag="P4G30_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_019725130.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replication initiation protein"
     gene            complement(1279..1554)
                     /locus_tag="P4G30_RS28460"
                     /old_locus_tag="P4G30_28420"
     CDS             complement(1279..1554)
                     /locus_tag="P4G30_RS28460"
                     /old_locus_tag="P4G30_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(1535..1780)
                     /locus_tag="P4G30_RS28465"
                     /old_locus_tag="P4G30_28425"
     CDS             complement(1535..1780)
                     /locus_tag="P4G30_RS28465"
                     /old_locus_tag="P4G30_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            2017..2178
                     /locus_tag="P4G30_RS28470"
                     /old_locus_tag="P4G30_28430"
     CDS             2017..2178
                     /locus_tag="P4G30_RS28470"
                     /old_locus_tag="P4G30_28430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            complement(2978..3379)
                     /locus_tag="P4G30_RS28475"
                     /old_locus_tag="P4G30_28435"
     CDS             complement(2978..3379)
                     /locus_tag="P4G30_RS28475"
                     /old_locus_tag="P4G30_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_019725133.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            3781..4395
                     /locus_tag="P4G30_RS28480"
                     /old_locus_tag="P4G30_28440"
     CDS             3781..4395
                     /locus_tag="P4G30_RS28480"
                     /old_locus_tag="P4G30_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_019725132.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
ORIGIN
        1 agttacaaga ggtctattct tactaaaaac tgtgtaaaca gacggaatgt aattagatat
       61 atcaacagta cacatcacgg caataaatgc taaaaaaact atagtccaaa gagttacgaa
      121 ggttttcatc ttatttcacc atttttcttg atataaaaat acttccgcct gcttattccc
      181 atagctaccc aaggttttat ttcctctagg cttcctttcc tccttgactg tcctgacttt
      241 ttccctctct ttgattgaat ttcagaggaa tgagtaatat caacaaattt actgaattct
      301 tcaggtgaaa atttatttga tgtccattta gcaatgctct tagctatccc tcttacctca
      361 ttttcatcca gaggccgtgc aaattgcagg ttgtatgcgc aagcgcgttc gtagcaggct
      421 tgcagccact gctcatactc aggccagccc tgacggatgg cgcgatatgc ccacttacgg
      481 gttttgtcga acaacgtgca gttacggcct aagccgtagt cggcaacgat ttccttgtca
      541 ttccctgtgt tcagatcgag aaagtcggcc agccagtcca gagtgtagag cttcggctgc
      601 cacacggcga ttttccagtg gttgtggttc ggatttttgc agatgagccc cgaatatcca
      661 gcgtcagcct ctaatttttt acgtagcgca ttctccacgg aggcggcgta gcgtaacggt
      721 tttattttgc cgtccggcgc ggtgcgtatc gagttttcca gcgcgtacag caggtgcgcg
      781 tgtccgtttt cggggttagt tatggtcagc gtcggtgcag gcgcgttgcg gtcgctccag
      841 tcgatggagg aaccggcgcg gtcaacgtca aatcccagcc agaacatggc gtgaggctga
      901 ttgaactgga tatatttcgc cagaatagcg cgttccttac cggcgatacg gacaccaaag
      961 tgcagatcgt ccgaaaagta cggcttgtgt ggtaagcgtt cgttgaaaag ttcgagtgcg
     1021 gcgttatgca atgtgccctg cgcttatggc gggctgttgt ctggcacggt tcatgtgcta
     1081 atatcctttc aaggtttcga cctagccctg gttatcatgc gatccgccaa gatatgacat
     1141 gatttccggg gctttgtttt ttctgaggct aacgttacac cgactttgcc tttgtgtcga
     1201 tctgacagcg acttacccta acatccgttg tacgccttgc atgaaaagcc cgcatcaagc
     1261 gggctatatc gacttttgct atttgtcacc atagtgataa cgggctgcgt agatgatgat
     1321 ttctccgttc tggattgaat agaccagacg gtggctctga tcaatcctgc gggagtagag
     1381 tgccggatct ttgtggtgcc gcagccgttc gggttttcct atacctgtca gcggatcagc
     1441 ctcgatgctg agcaacagtt gccggatacg ctcaaacttc tttttgtcag tatcacgcca
     1501 gtagttaagg tcatcagcgc cattcctagt gaacttaata ttccacttcg acaaattcgc
     1561 cgcgctccgc ctgtttcatt gactcattga tatgctctgc gttcgccgga ttgctgaaca
     1621 gatagaccgt ttcgattagc gcctcatact ctcccgcatc gatcataaca atatcaggcg
     1681 catcacggcg catgacacgt accggttcag catcatcagt gacgcgcttc atcgtctcgg
     1741 caaaatgttt acgggcatca gtaaaattga ttgcatgcat gttgttaacc cccatttgtt
     1801 ggggcttagc atagaacatg tacgcaaata agtacataaa gatatttccc taaaaaccca
     1861 caaccaaatc agctcaaaat gtgaactcaa tcgaataacc acaaaaacaa caagtcaata
     1921 cattgaagca taatttcaag caattacatt tatgaagtgc ggcacaattt cgtaccgcta
     1981 ggagcttagc tcctaaaacg agcaaaggaa aagtaaatgg caaaacattg gtctgggcaa
     2041 gaatgtccaa aaacaggtac ttacggtcaa tatcatgata ccgacaactc atacgctggt
     2101 tctgaatatg atcgccatgt gagcaagggt gataagttcc ctccgagcaa aaacaaccac
     2161 cacttccaag aaaaataaaa tatcttcaga agtctaaact aaaagccctc tggggctttt
     2221 tttgtacctg ccattcactt gatttaccat aacatccgtt atcagcaccg cctgagatgc
     2281 cctcagggcg tttctgacgg gaaaccggta aacaaggcaa caaaccaccc gaaaacccgc
     2341 cagaaacgcg caggctctgt ttttacggca tgcatgagta tgcgcacaag tgcatagcgc
     2401 ggaaattgac accattcagc caggggaagt cctgtttccg gcgttcttcc catccgaaaa
     2461 cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa
     2521 ccggttttca gcacgttcag gaatcggttc gtccacggcc ggcaatcacg ttatggcaag
     2581 tcgccggacg actcaccaca cgattccccg gcgactcttc actagagtcg tacccagagt
     2641 cgccacgtct aaaaaacaac caaacgggga gtcggatcta gagtcgggtc gttattaact
     2701 cattgttttt ataggcctga gtcgttaccg actctatgac gactcgccgg ggactctatg
     2761 acgactcgtc atagagtcgg atctatataa ataaaattta aaaaacgaaa tttcagtaaa
     2821 gtaagctgct gatattaaat acaaatctgc aaaattaaaa ttttgcgtgg ggtgtgggta
     2881 tttttcgtgg tgaggagcgt tcagagatct gacgattttc gggaaggttc aggaactccc
     2941 cggaatggcc ggagaggaaa actgaagcat gacggggtca ggattttttc cagaatttcc
     3001 accacggcgc cgggggttca gttaattttg cctggtgatt ttcaagaagg agcatggcct
     3061 gtttcaggct gtcaatgtgc gaatccttcg ataaaatcaa tatgttaagg tgttcaatct
     3121 gctgtttcag ttgacgaatg acatgttcac tctggtcagc aggcgctgta tcacgctgtt
     3181 caggtacttg tcgagtaagg tgttcattct tcggttggtc aaacggtccg aaaacacgta
     3241 aaagctctga tatatcgacc atttttgtat ttcgttcacc gctacaagtg gacagttcac
     3301 ctgttttgat taacctgtgc agcgttgtcc ggctcttacc cgtcagccgt gcagcctcac
     3361 taatagacac tctggtcatc agttatcatc ctttaataca ttcagtctca aggaaacagg
     3421 ggccagctta cgacatactg gtccagaagt gatgtgtttt tcagaagggc gatgaaacac
     3481 aggagtgacc gtgcaaaatg caggcaacaa atgactttcc gcttcctcgc gcattcgctc
     3541 actacgttcg gtcataagac tgcggcgagc ggtacgtaag aataaagtga ggtgatagct
     3601 ctcttctcaa ggcgtttagg gaagcaacga gcccgcccta aaccagtcat ggcgagacct
     3661 tgctgaaggg accctatgtg tgctggctga gaaaaaaaga gctctctaaa agagagctgt
     3721 tatctgatat aggttcaagt gcaaaataga gtgcacacaa acaaaaaaag gacaataaaa
     3781 atgggaaagc ataaggtatt cattagctac caccatgcga atgatcagta ctacaaaaac
     3841 gcacttgaaa aaatgaatga ggaacatgaa atattcgtga atcgctcagt atcattaggt
     3901 gatatagatg aagatgaagc cccacaaaaa ataagagaga taataagaga tgaatatctt
     3961 agagatacat cagttcttat ccttcttgtt ggaactgaaa caaaaaacag aaaacacgta
     4021 gattgggaac tttactctag catgagagat agtaccatta acaaaaaatc aggaatttta
     4081 gtcgtcaatc tcccgtcaac aaatactacg tacattcgtt caacacatgg aactaatgaa
     4141 aaatcagaag ttcatcctac cgtaactgaa tggttttcta ttgataagcg ctcaacttat
     4201 gaggaaaggt tcccatatat gccagcaaga ataatagaca acttcatgaa gagcgggtct
     4261 tatatatcag tggtcaactg gaatcaaata gaaaacaacc cagaaacact cagaaaatta
     4321 atagacatga cttataacga tagagaaaaa gcaacgtatg atatgtcgag agaaatgaga
     4381 atgcataatt catagtacta tccaaggagt agccttggct actcctttaa tttaccagtt
     4441 ccacttatct gtattgattc caaacttagg cttcacatca ttaacaaaga accggttaac
     4501 atctttaaaa ccttttaaaa tacgctcata atcataatac tgcatttgta ttttatttat
     4561 gctggttgca tgaaatctca tgacatcacc gctaacccta tcaacaacct catctttgtc
     4621 tgatatgtac ttataccaac gcttgtagtt gttctggtag ttagttagaa atctatttaa
     4681 caccatgagc atctcattgc tgaaatcata aatctcacga tcttttttag ggtttagtat
     4741 tttcatttca tcacgaataa attggtaagt ctgataatat gatgaaataa cctcatcaaa
     4801 ggcatcaacg ttttttctta atttgaaaat tgctcttttt gaatctaggt gattttttac
     4861 actctttttg taaagtgtag cactctcatt gaaaagtata ttcactccac caaggctaag
     4921 tttctcaacc ttaaccgaat actttttaag taagtagagg gataccatca gccaaataaa
     4981 aacagatatc aaaagaatag aaagggcgcc
//
