LOCUS       NZ_CP125131             3511 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 181 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP125131
VERSION     NZ_CP125131.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790399
            Assembly: GCF_037477755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 64x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477755.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:30:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,724
            CDSs (total)                      :: 5,599
            Genes (coding)                    :: 5,462
            CDSs (with protein)               :: 5,462
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 54 of 137
            Pseudo Genes (incomplete)         :: 80 of 137
            Pseudo Genes (internal stop)      :: 22 of 137
            Pseudo Genes (multiple problems)  :: 17 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125131.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="181"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            join(3202..3511,1..146)
                     /locus_tag="P4G31_RS28600"
                     /old_locus_tag="P4G31_28560"
     CDS             join(3202..3511,1..146)
                     /locus_tag="P4G31_RS28600"
                     /old_locus_tag="P4G31_28560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_249415993.1"
                     /translation="MTGSGLRPETPLTRLRLVQPRADRERLSRPSRRYQEAILADKRNK
                     MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTRAGRLPSISPALLRQLAGMGNN
                     LNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            355..>783
                     /locus_tag="P4G31_RS28605"
                     /old_locus_tag="P4G31_28565"
                     /pseudo=""
     CDS             355..>783
                     /locus_tag="P4G31_RS28605"
                     /old_locus_tag="P4G31_28565"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            824..1321
                     /locus_tag="P4G31_RS28610"
                     /old_locus_tag="P4G31_28570"
     CDS             824..1321
                     /locus_tag="P4G31_RS28610"
                     /old_locus_tag="P4G31_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            1328..1543
                     /locus_tag="P4G31_RS28615"
                     /old_locus_tag="P4G31_28575"
     CDS             1328..1543
                     /locus_tag="P4G31_RS28615"
                     /old_locus_tag="P4G31_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1938..2120
                     /locus_tag="P4G31_RS28620"
                     /old_locus_tag="P4G31_28580"
     CDS             1938..2120
                     /locus_tag="P4G31_RS28620"
                     /old_locus_tag="P4G31_28580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          3058..3231
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2570..3091)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          137..1659
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3338..3510
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.828"
                     /coverage="98.0"
                     /evalue="1.55e-30"
ORIGIN
        1 acctgaacca gatcgcccgg aaagtgaacg ccggtggtgc cggtcatgac cgggtgcaga
       61 tcgtggcagc gctgatggcg atcgatgccg gtctggaacg gttgcggcat gccgtactgg
      121 aaaaagggcc ggacgatgat tgttaaattc catccccggg gtcgcggcgg gggcgccggt
      181 cctgtggatt atcttctggg taaagaccgg cagcgtgaag gtgccagcgt cctgcagggg
      241 aagccggaag aagtccggga gctgattgat gcgtcgccgt atgtgaaaaa atatacctcc
      301 ggggtgctgt cattcgcgga ggcggacctg ccgccgggtc agcgtgaaaa actgatggcc
      361 agctttgagc gggttctgat gcccggactc gataaagacc agtacagcat tctgtgggtg
      421 gaacacgcag acaagggtcg tcttgagctg aattttctga tccccaatac cgaattactg
      481 acggggaaac gcctccagcc gtattacgat cgtgccgacc ggccgcgcat cgatgcgtgg
      541 cagacagtgg tcaacggcag gctggggcta catgatccga acgacccgga gaaccggcgg
      601 gcgctggtca cgccttcagg actgccagaa acgaagcagg aagccgcaga gacgattaca
      661 cggggtttgc tgatgttagc ctcatcagga gagctgaaaa cgcgtgatga cgtcacaggg
      721 gcgctcacag cggccggttt tgaggtggtc agaaccacaa aaaacagtat cagcattgcc
      781 gacccggacg gggggcgaaa tatccgactg aggggagcca tttatgagca gtcttttaac
      841 ggcctcgagg gacttcgagg agaaatcgaa agcacagcag cagcttaccg aagagaggct
      901 gaaagccgca ttcagcaagc acgagaacgt tgtcaaatcg gaactgaacg caagcgcgaa
      961 gagaatcaac gacgccatca gcgcccacga acaggggatg aacgcggcga tgcagtcgaa
     1021 ccggctgagc gtgctgcgaa tggtcgggcg gacgtggctg accatcacca tggtgtcagg
     1081 gctgctgttc gccagcctga gcggggtgct gtggtaccag gggaaccgga tagcggcgaa
     1141 cctggcggaa atcaggcagc agagagatac gctgtcgaaa ctgcacatgc agacgtgggg
     1201 cgtgacctat ctggagaacc gcaacggacg gtttctggtg ctgccggagg ggatgaaagc
     1261 cgaaacgggc cggacggtgg acaacaagac gcggaacgcg gtgaagctgg tacgggagta
     1321 gagcagaatg acggaactgg aaaaacaatt gctgagcgcg ttagagcagc tacagcagga
     1381 ctactcgaga aggctggacg agtgggagag cgccttcgag gaatggcgga agatgtctgg
     1441 tcttatgcaa cgggagaacg cggcgctgag cgagcgcgtg tcgggcttga gcaggcaggt
     1501 gcagagtttg agcgagcagc tgcgccggtt atcgacaggc tgaatgagat tgagcaacag
     1561 cgatacgggc aaacaacgga aaaggaacca gagaagacag cagatctggt accggaacgg
     1621 caacgcacat tatctcatgg tgacagggag tatggagact gataaacgcc cgtctcaggt
     1681 ggcctgagcg tgacggggat catggccatt ttctgcgatt tcagcacaca gagagtcttt
     1741 tttaaccgaa atcaccggct atgcagtgat gtgaataagt gatgcatatg cgtaaaataa
     1801 atcaggtgcg tttctggtgg ttttcggtgt acttgccacc accttttgcc cgtcagccat
     1861 cagaaacgcc ctgagtgcct ccggcgcggt gcgggtaaga gatgttatgt ttaatgttga
     1921 taacaggagt aagagtgatg cttacattta ttggtgtagt ggtagtcgcc gttattgcat
     1981 ttaaattatt tatgcttttt tttagttcat ccgaatctgc cgtcgaacgt gctacccgta
     2041 aatatatgga gaaccctacg agtgctaact ataaaataat ggtggctaca cgcatgagaa
     2101 atgagaagaa aagagggtga tatatgtttg agtttattgg ttttttattt ttacttttgg
     2161 tgtgttatgt ctttatacaa atggttgctg tcagggtgtt tcctgaatat ggagtacgga
     2221 aagcggagga acgataccgt cgaaatccat cagcagaaaa tgaggatctg ttatggagag
     2281 ctagaagtcg tataactaaa cgcaagtaat gtttcctaag tgtgttgata ggcaaacatg
     2341 gtgggagtta ctactctcgg agttaatgat cttcttgtga tcctgtcgtt tcagtcgtaa
     2401 tctcttgctc tgaaaacgaa aaaaccacct ggggaggtgg tttgatcgaa ggttaagtct
     2461 gttggggaac tgcttaacca ggtaactggg tatacgagac cgcagatacc aaaactgttc
     2521 tttcagtgta gccgcagtca ggcccatact tcaagacctc tgaatctctc tctcgtatat
     2581 cccagttacc agtggctgct gccagtggcg ctttgtcgtg ctttctcggg ttggactcaa
     2641 gatgatagtt accgggtaag gcgcagcagt cggactgaac ggggggtttg tgcatacagt
     2701 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agataaacgc
     2761 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     2821 gagccgccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
     2881 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttc
     2941 gccgcggccc tatcgcttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc
     3001 cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga
     3061 gcgaggaagc ggaatatatc ctgtatcaca tattctgctg acgcgccggt gccgcctttt
     3121 ttctcctgcc acatgaagca cttcactgac atcctcatcc gtgccaacat agtaagccag
     3181 tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac cccactgacg
     3241 cgcctgcggc ttgtccaacc ccgcgccgac cgggaaaggc tttcccgtcc gtcacggcgt
     3301 tatcaggagg cgattctggc agataaacgc aacaagatgc tcacgatgtg ggtgacggaa
     3361 gacgaacacc ggcggttgct tgagcggtgc gacggaaaac aactggcggc gtggatgcgc
     3421 cagacctgcc ttgatgagaa accgacacgc gccggcaggc ttccctcgat ttcgcccgcg
     3481 ctgctgcgtc agcttgccgg catgggcaat a
//
