LOCUS       NZ_CP125130             4052 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 181 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP125130
VERSION     NZ_CP125130.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790399
            Assembly: GCF_037477755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 64x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477755.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:30:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,724
            CDSs (total)                      :: 5,599
            Genes (coding)                    :: 5,462
            CDSs (with protein)               :: 5,462
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 54 of 137
            Pseudo Genes (incomplete)         :: 80 of 137
            Pseudo Genes (internal stop)      :: 22 of 137
            Pseudo Genes (multiple problems)  :: 17 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125130.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="181"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            104..445
                     /locus_tag="P4G31_RS28575"
                     /old_locus_tag="P4G31_28535"
     CDS             104..445
                     /locus_tag="P4G31_RS28575"
                     /old_locus_tag="P4G31_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            654..>1085
                     /locus_tag="P4G31_RS28580"
                     /old_locus_tag="P4G31_28540"
                     /pseudo=""
     CDS             654..>1085
                     /locus_tag="P4G31_RS28580"
                     /old_locus_tag="P4G31_28540"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1123..1608
                     /locus_tag="P4G31_RS28585"
                     /old_locus_tag="P4G31_28545"
     CDS             1123..1608
                     /locus_tag="P4G31_RS28585"
                     /old_locus_tag="P4G31_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_180160425.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNAKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            1612..1824
                     /locus_tag="P4G31_RS28590"
                     /old_locus_tag="P4G31_28550"
     CDS             1612..1824
                     /locus_tag="P4G31_RS28590"
                     /old_locus_tag="P4G31_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2332..2790)
                     /locus_tag="P4G31_RS28595"
                     /old_locus_tag="P4G31_28555"
     CDS             complement(2332..2790)
                     /locus_tag="P4G31_RS28595"
                     /old_locus_tag="P4G31_28555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     Typing          complement(3427..3907)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          436..1940
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.024"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccccgaaac ccgctaaaag ccactgacgc gcctgcggct tgtccaacac cgtgccgacc
       61 gggaaacatt tcccgcccgt cccggtgtta tcaggaggct actctggcag acaaacgcaa
      121 caagatgctc acgatgtggg tgacggaaga cgaacaccgg cggctgctcg agcggtgcga
      181 cggcaggcag ctggcggcgt ggatgcgtca gtcctgcctc gatgagaagc cggcgcgtgc
      241 ctgcagactg ccgtcaatat cgccggcgct gctgcgccag cttgccggta tgggtaacaa
      301 cctgaaccag atcgcccgga aagtgaatgc cgttggtgcc ggtcatgatc gggtgcagat
      361 tgtggccgcc ctgatggcaa tagacgcggg actggagcgg ctgcggcatg cggtgctgga
      421 aaaagggggg gatgatgatc gttaaatttc atccccgggg gcgcggtggt ggtgccggtc
      481 cggtggatta cctgctggga aaagaccggc agcgcgaggg ggcgaccgtg ctgcagggaa
      541 aaccggagga agtccgggag cttatcgatg cctcgccgta tgcgaaaaag tacacctccg
      601 gcgtgctgtc gttcgcggag gctgaactgc tgcccgggca gcgggagcag atcatggcca
      661 gctttgagcg ggttctgatg cccgggctcg ataaagacca gtacagcatt ctgtgggtgg
      721 agcacaccga caaggggcgg ctggagctga atttcctgat ccccaacact gaactgctga
      781 ccgggaaacg cctgcagccg tattacgatc gcgccgaccg gccgcgcatc gatgcctggc
      841 agaccgtggt gaacggcaga ctgggactgc atgatccaaa cgccccggag aaccggcggt
      901 tactggttac gccgtccgca ctgccggaaa cgaagctgga agccgctcag gcgattacac
      961 gtgggttgct ggccttagcc tcatccgggg agctcaaaac tcgcgaggac gtcacaggtg
     1021 cgctcacagc ggcaggtttt gaggtggtcc gcactacaaa aaacagtatc agcattgccg
     1081 acccggacgg ggggcgaaac atccgactga agggagccat ttatgagcag tcttttagcg
     1141 ctggcgaagg acttggagca gcaatcgaaa gcgcagcagc agagtaccgg cgagatgctg
     1201 aaagccgcat tcagcgagca cgagaaatct gtaaaagcgg aactgagcgc aagcgcgaag
     1261 agaatcagcg acgccatcag cgcccacgag cagggtatga ccgcggcgat gcagtcgaac
     1321 cgcctgagcg tgctgcgtat ggtcggccgg acgtggctga ccatcaccct ggtctcggta
     1381 ctgctgatcg ccatgagcgg gagcattctg tggtggcagg ggcagcagat caccgggaat
     1441 taccagacta tccgggcgca ggagcggacg caggccatgt tgtcggagaa aaaccacggc
     1501 gttcagctgt cgctctgcgg ggagaagaaa ctgtcctgcg tcaaagtgaa cgcgaaagcg
     1561 ggagcttacg gggaggaagg gaactggatg gtactggaga ggaaatagcc catgacggaa
     1621 ctggaaaaac agttgctgac cgcattagag cagctacatc aggactactc gcaaaggctg
     1681 gacgagtggg agagcgcctt cgcggaatgg cagaagatgt ctggtcttat gcaacgggag
     1741 aacgcggcgc tgaacgagcg cgtcacgcgc ttgagtcagc aggtggcgaa cttgagcagg
     1801 cagttacagc gtttgagcca gtagtgcagc ggcatgaaat ggcggtggcc gccgaacggg
     1861 catacgagca gcgacagcac gaaaaagagc tggcggaagc ccgttcccgt cagcggagct
     1921 ataacggtcc gacgctgggc tgacaggctt caccagtgac aaaccccacg aagaagggct
     1981 cataaatttc ccgctaaata cggtcgtgat gaaaacatca cggcgtatac aacggaggga
     2041 ttatgagact gtttaacgtg attgagacgg tgctgaactg cctgtacatg gcgctggaga
     2101 tctgggactg gctggagaag cattttcctg tttttaaggc gttcatccag attctggcct
     2161 gaaatcagac cgtatgcatc tggctgcatg accatgcagc cgggtaaaat ttatctgtca
     2221 cgaacggggc ggtgtatcgg gtggtttgtt gccggtttta cctgactgcc gccccgtttg
     2281 tgacgaacat gtccggagcg gtgcgggcaa gagatgttat ggtaaatcat attagttctg
     2341 aatgattcgg gctccaccag tcattactat ttgagcacca cctgacattt ttatacttgt
     2401 accctggcta gtcattgtcg tatcaccttg tatgatcgtt tttccagtag catgttgacc
     2461 tccaccagtg actaaaattg acgcttgtac ttgagatcca tttatcaaaa cagttccggc
     2521 ttggtgatca aattttattc cgctcaatgc ttttaattca gctccatcta gctgaatttc
     2581 accattggct gctgcagcct gaatggcccc ctgcatctga gaaaataatt tatcaacttc
     2641 gtcaaaaggt aatggtgttt caagcagtga ggcttgttgt tcaacttcag gatgtttact
     2701 tgctaacttc cctatgatgc ctttgattgc atcagaagta agattgctta atgcgccagt
     2761 gagaacggca agtagtgtgg tttcaatcat tatatttccc tcgttagatt gctgtgatgc
     2821 gtattattta ctctttataa tctccgattc tgtccatatg gttagttgca ctattacgtt
     2881 tataagtgcc acttttacca tatatatatc caacgctaca ttgtggacat tttggcgtct
     2941 cgtcattatg agtgaaattt atttctcctc cgacagatag ggtttttacg ttttccggat
     3001 aatctaatgt agccacgccg gtccattcac aattcgagca gacaacatcg aaagatgttg
     3061 ggatactctt gcagttgatg ttgatttgca tatgcataac ctcgattttg cacagaaatt
     3121 acagtgttat tagttgcata ccgactataa cctgagtttg tgatggagtg gtgttttttt
     3181 gctttgactt gagtaagaga aagagatgat cttcttgaga taatctcttt ctgagcgtag
     3241 tcttttgctc tgtaaacgaa aaaaccaccc tggcaggtgg tttttcgaag gttagttaat
     3301 cctggcagat ttactaaccg tggtaacagt cttgtgcgag acatgtcacc aaagctgtcc
     3361 ttccagtgta gccgcagtaa ggccaccact tcaagaactc tgaatacatc tctcgcacat
     3421 cctgtttacc agtggctact gccaatggcg atttgtcgtg ccttttcggg ttggactcaa
     3481 gacgatagtt accgaaaaga gcgtggtagt cggactgaac ggggggatcg tgcatacagt
     3541 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc
     3601 ggccataaca gcggaatgac accggtaaac cgaatggcag gaacaggaga gcgcacgagg
     3661 gagctaccag ggggaaacgc ctgggatctt tatagtcctg tcgggtttcg ccaccactga
     3721 tttgagcgtc agattttatg atactcgtca ggggggcgga gcctatggaa aaacggcaac
     3781 gccgcatcct cttcccggct tctcctgatt tgagcgctgc ttgcgtatcc cgtcatctgt
     3841 gagctggtaa cgcccgccgc agtctcacgg caggagcgca gcgactgagt gagcgaggag
     3901 gcgtcatttc acctttacca gcatgttgca cttacgcccg tcagccacat tttccccttc
     3961 ccacatgaag cactttcata cattaccact ccgccgtaac atatttagcc agtatacact
     4021 ccgctagcgc tacgtgactg gttcagggct gc
//
