LOCUS       NZ_CP125125             3511 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 189 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP125125
VERSION     NZ_CP125125.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790402
            Assembly: GCF_037477845.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477845.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:31:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,719
            CDSs (total)                      :: 5,595
            Genes (coding)                    :: 5,455
            CDSs (with protein)               :: 5,455
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 54 of 140
            Pseudo Genes (incomplete)         :: 87 of 140
            Pseudo Genes (internal stop)      :: 19 of 140
            Pseudo Genes (multiple problems)  :: 17 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125125.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="189"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(join(3275..3511,1..84))
                     /locus_tag="P4G34_RS28575"
                     /old_locus_tag="P4G34_28540"
     CDS             complement(join(3275..3511,1..84))
                     /locus_tag="P4G34_RS28575"
                     /old_locus_tag="P4G34_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015328236.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTRAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGPDD
                     DC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1301..1483)
                     /locus_tag="P4G34_RS28580"
                     /old_locus_tag="P4G34_28545"
     CDS             complement(1301..1483)
                     /locus_tag="P4G34_RS28580"
                     /old_locus_tag="P4G34_28545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            complement(1878..2093)
                     /locus_tag="P4G34_RS28585"
                     /old_locus_tag="P4G34_28550"
     CDS             complement(1878..2093)
                     /locus_tag="P4G34_RS28585"
                     /old_locus_tag="P4G34_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2100..2597)
                     /locus_tag="P4G34_RS28590"
                     /old_locus_tag="P4G34_28555"
     CDS             complement(2100..2597)
                     /locus_tag="P4G34_RS28590"
                     /old_locus_tag="P4G34_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2638..3336)
                     /locus_tag="P4G34_RS28595"
                     /pseudo=""
     CDS             complement(<2638..3336)
                     /locus_tag="P4G34_RS28595"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(191..364)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          331..852
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1763..3285)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3282..3511)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.744"
                     /coverage="99.0"
                     /evalue="7.679999999999999e-38"
ORIGIN
        1 gcgcatccac gccgccagtt gttttccgtc gcaccgctca agcaaccgcc ggtgttcgtc
       61 ttccgtcacc cacatcgtga gcatcttgtt gcgtttatct gccagaatcg cctcctgata
      121 acgccgtgac ggacgggaaa gcctttcccg gtcggcgcgg ggttggacaa gccgcaggcg
      181 cgtcagtggg gtttcggggc gcagccctga accagtcacg tagcgctagc ggagtgtata
      241 ctggcttact atgttggcac ggatgaggat gtcagtgaag tgcttcatgt ggcaggagaa
      301 aaaaggcggc accggcgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc
      361 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg
      421 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagcgata gggccgcggc
      481 gaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa
      541 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc
      601 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
      661 gcgtttatct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
      721 actgtatgca caaacccccc gttcagtccg actgctgcgc cttacccggt aactatcatc
      781 ttgagtccaa cccgagaaag cacgacaaag cgccactggc agcagccact ggtaactggg
      841 atatacgaga gagagattca gaggtcttga agtatgggcc tgactgcggc tacactgaaa
      901 gaacagtttt ggtatctgcg gtctcgtata cccagttacc tggttaagca gttccccaac
      961 agacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgttttca gagcaagaga
     1021 ttacgactga aacgacagga tcacaagaag atcattaact ccgagagtag taactcccac
     1081 catgtttgcc tatcaacaca cttaggaaac attacttgcg tttagttata cgacttctag
     1141 ctctccataa cagatcctca ttttctgctg atggatttcg acggtatcgt tcctccgctt
     1201 tccgtactcc atattcagga aacaccctga cagcaaccat ttgtataaag acataacaca
     1261 ccaaaagtaa aaataaaaaa ccaataaact caaacatata tcaccctctt ttcttctcat
     1321 ttctcatgcg tgtagccacc attattttat agttagcact cgtagggttc tccatatatt
     1381 tacgggtagc acgttcgacg gcagattcgg atgaactaaa aaaaagcata aataatttaa
     1441 atgcaataac ggcgactacc actacaccaa taaatgtaag catcactctt actcctgtta
     1501 tcaacattaa acataacatc tcttacccgc accgcgccgg aggcactcag ggcgtttctg
     1561 atggctgacg ggcaaaaggt ggtggcaagt acaccgaaaa ccaccagaaa cgcacctgat
     1621 ttattttacg catatgcatc acttattcac atcactgcat agccggtgat ttcggttaaa
     1681 aaagactctc tgtgtgctga aatcgcagaa aatggccatg atccccgtca cgctcaggcc
     1741 acctgagacg ggcgtttatc agtctccata ctccctgtca ccatgagata atgtgcgttg
     1801 ccgttccggt accagatctg ctgtcttctc tggttccttt tccgttgttt gcccgtatcg
     1861 ctgttgctca atctcattca gcctgtcgat aaccggcgca gctgctcgct caaactctgc
     1921 acctgcctgc tcaagcccga cacgcgctcg ctcagcgccg cgttctcccg ttgcataaga
     1981 ccagacatct tccgccattc ctcgaaggcg ctctcccact cgtccagcct tctcgagtag
     2041 tcctgctgta gctgctctaa cgcgctcagc aattgttttt ccagttccgt cattctgctc
     2101 tactcccgta ccagcttcac cgcgttccgc gtcttgttgt ccaccgtccg gcccgtttcg
     2161 gctttcatcc cctccggcag caccagaaac cgtccgttgc ggttctccag ataggtcacg
     2221 ccccacgtct gcatgtgcag tttcgacagc gtatctctct gctgcctgat ttccgccagg
     2281 ttcgccgcta tccggttccc ctggtaccac agcaccccgc tcaggctggc gaacagcagc
     2341 cctgacacca tggtgatggt cagccacgtc cgcccgacca ttcgcagcac gctcagccgg
     2401 ttcgactgca tcgccgcgtt catcccctgt tcgtgggcgc tgatggcgtc gttgattctc
     2461 ttcgcgcttg cgttcagttc cgatttgaca acgttctcgt gcttgctgaa tgcggctttc
     2521 agcctctctt cggtaagctg ctgctgtgct ttcgatttct cctcgaagtc cctcgaggcc
     2581 gttaaaagac tgctcataaa tggctcccct cagtcggata tttcgccccc cgtccgggtc
     2641 ggcaatgctg atactgtttt ttgtggttct gaccacctca aaaccggccg ctgtgagcgc
     2701 ccctgtgacg tcatcacgcg ttttcagctc tcctgatgag gctaacatca gcaaaccccg
     2761 tgtaatcgtc tctgcggctt cctgcttcgt ttctggcagt cctgaaggcg tgaccagcgc
     2821 ccgccggttc tccgggtcgt tcggatcatg tagccccagc ctgccgttga ccactgtctg
     2881 ccacgcatcg atgcgcggcc ggtcggcacg atcgtaatac ggctggaggc gtttccccgt
     2941 cagtaattcg gtattgggga tcagaaaatt cagctcaaga cgacccttgt ctgcgtgttc
     3001 cacccacaga atgctgtact ggtctttatc gagtccgggc atcagaaccc gctcaaagct
     3061 ggccatcagt ttttcacgct gacccggcgg caggtccgcc tccgcgaatg acagcacccc
     3121 ggaggtatat tttttcacat acggcgacgc atcaatcagc tcccggactt cttccggctt
     3181 cccctgcagg acgctggcac cttcacgctg ccggtcttta cccagaagat aatccacagg
     3241 accggcgccc ccgccgcgac cccggggatg gaatttaaca atcatcgtcc ggcccttttt
     3301 ccagtacggc atgccgcaac cgttccagac cggcatcgat cgccatcagc gctgccacga
     3361 tctgcacccg gtcatgaccg gcaccaccgg cgttcacttt ccgggcgatc tggttcaggt
     3421 tattgcccat gccggcaagc tgacgcagca gcgcgggcga aatcgaggga agcctgccgg
     3481 cgcgtgtcgg tttctcatca aggcaggtct g
//
