LOCUS       NZ_CP125124             4052 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 189 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP125124
VERSION     NZ_CP125124.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790402
            Assembly: GCF_037477845.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477845.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:31:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,719
            CDSs (total)                      :: 5,595
            Genes (coding)                    :: 5,455
            CDSs (with protein)               :: 5,455
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 54 of 140
            Pseudo Genes (incomplete)         :: 87 of 140
            Pseudo Genes (internal stop)      :: 19 of 140
            Pseudo Genes (multiple problems)  :: 17 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125124.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="189"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(join(4047..4052,1..453))
                     /locus_tag="P4G34_RS28550"
                     /old_locus_tag="P4G34_28515"
     CDS             complement(join(4047..4052,1..453))
                     /locus_tag="P4G34_RS28550"
                     /old_locus_tag="P4G34_28515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            1819..2160
                     /locus_tag="P4G34_RS28555"
                     /old_locus_tag="P4G34_28520"
     CDS             1819..2160
                     /locus_tag="P4G34_RS28555"
                     /old_locus_tag="P4G34_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2369..>2800
                     /locus_tag="P4G34_RS28560"
                     /old_locus_tag="P4G34_28525"
                     /pseudo=""
     CDS             2369..>2800
                     /locus_tag="P4G34_RS28560"
                     /old_locus_tag="P4G34_28525"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2838..3323
                     /locus_tag="P4G34_RS28565"
                     /old_locus_tag="P4G34_28530"
     CDS             2838..3323
                     /locus_tag="P4G34_RS28565"
                     /old_locus_tag="P4G34_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_180160425.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNAKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            3327..3539
                     /locus_tag="P4G34_RS28570"
                     /old_locus_tag="P4G34_28535"
     CDS             3327..3539
                     /locus_tag="P4G34_RS28570"
                     /old_locus_tag="P4G34_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1553..1732
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          complement(1090..1570)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          2151..3655
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.024"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgaatgatt cgggctccac cagtcattac tatttgagca ccacctgaca tttttatact
       61 tgtaccctgg ctagtcattg tcgtatcacc ttgtatgatc gtttttccag tagcatgttg
      121 acctccacca gtgactaaaa ttgacgcttg tacttgagat ccatttatca aaacagttcc
      181 ggcttggtga tcaaatttta ttccgctcaa tgcttttaat tcagctccat ctagctgaat
      241 ttcaccattg gctgctgcag cctgaatggc cccctgcatc tgagaaaata atttatcaac
      301 ttcgtcaaaa ggtaatggtg tttcaagcag tgaggcttgt tgttcaactt caggatgttt
      361 acttgctaac ttccctatga tgcctttgat tgcatcagaa gtaagattgc ttaatgcgcc
      421 agtgagaacg gcaagtagtg tggtttcaat cattatattt ccctcgttag attgctgtga
      481 tgcgtattat ttactcttta taatctccga ttctgtccat atggttagtt gcactattac
      541 gtttataagt gccactttta ccatatatat atccaacgct acattgtgga cattttggcg
      601 tctcgtcatt atgagtgaaa tttatttctc ctccgacaga tagggttttt acgttttccg
      661 gataatctaa tgtagccacg ccggtccatt cacaattcga gcagacaaca tcgaaagatg
      721 ttgggatact cttgcagttg atgttgattt gcatatgcat aacctcgatt ttgcacagaa
      781 attacagtgt tattagttgc ataccgacta taacctgagt ttgtgatgga gtggtgtttt
      841 tttgctttga cttgagtaag agaaagagat gatcttcttg agataatctc tttctgagcg
      901 tagtcttttg ctctgtaaac gaaaaaacca ccctggcagg tggtttttcg aaggttagtt
      961 aatcctggca gatttactaa ccgtggtaac agtcttgtgc gagacatgtc accaaagctg
     1021 tccttccagt gtagccgcag taaggccacc acttcaagaa ctctgaatac atctctcgca
     1081 catcctgttt accagtggct actgccaatg gcgatttgtc gtgccttttc gggttggact
     1141 caagacgata gttaccgaaa agagcgtggt agtcggactg aacgggggga tcgtgcatac
     1201 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa
     1261 cgcggccata acagcggaat gacaccggta aaccgaatgg caggaacagg agagcgcacg
     1321 agggagctac cagggggaaa cgcctgggat ctttatagtc ctgtcgggtt tcgccaccac
     1381 tgatttgagc gtcagatttt atgatactcg tcaggggggc ggagcctatg gaaaaacggc
     1441 aacgccgcat cctcttcccg gcttctcctg atttgagcgc tgcttgcgta tcccgtcatc
     1501 tgtgagctgg taacgcccgc cgcagtctca cggcaggagc gcagcgactg agtgagcgag
     1561 gaggcgtcat ttcaccttta ccagcatgtt gcacttacgc ccgtcagcca cattttcccc
     1621 ttcccacatg aagcactttc atacattacc actccgccgt aacatattta gccagtatac
     1681 actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgct aaaagccact
     1741 gacgcgcctg cggcttgtcc aacaccgtgc cgaccgggaa acatttcccg cccgtcccgg
     1801 tgttatcagg aggctactct ggcagacaaa cgcaacaaga tgctcacgat gtgggtgacg
     1861 gaagacgaac accggcggct gctcgagcgg tgcgacggca ggcagctggc ggcgtggatg
     1921 cgtcagtcct gcctcgatga gaagccggcg cgtgcctgca gactgccgtc aatatcgccg
     1981 gcgctgctgc gccagcttgc cggtatgggt aacaacctga accagatcgc ccggaaagtg
     2041 aatgccgttg gtgccggtca tgatcgggtg cagattgtgg ccgccctgat ggcaatagac
     2101 gcgggactgg agcggctgcg gcatgcggtg ctggaaaaag ggggggatga tgatcgttaa
     2161 atttcatccc cgggggcgcg gtggtggtgc cggtccggtg gattacctgc tgggaaaaga
     2221 ccggcagcgc gagggggcga ccgtgctgca gggaaaaccg gaggaagtcc gggagcttat
     2281 cgatgcctcg ccgtatgcga aaaagtacac ctccggcgtg ctgtcgttcg cggaggctga
     2341 actgctgccc gggcagcggg agcagatcat ggccagcttt gagcgggttc tgatgcccgg
     2401 gctcgataaa gaccagtaca gcattctgtg ggtggagcac accgacaagg ggcggctgga
     2461 gctgaatttc ctgatcccca acactgaact gctgaccggg aaacgcctgc agccgtatta
     2521 cgatcgcgcc gaccggccgc gcatcgatgc ctggcagacc gtggtgaacg gcagactggg
     2581 actgcatgat ccaaacgccc cggagaaccg gcggttactg gttacgccgt ccgcactgcc
     2641 ggaaacgaag ctggaagccg ctcaggcgat tacacgtggg ttgctggcct tagcctcatc
     2701 cggggagctc aaaactcgcg aggacgtcac aggtgcgctc acagcggcag gttttgaggt
     2761 ggtccgcact acaaaaaaca gtatcagcat tgccgacccg gacggggggc gaaacatccg
     2821 actgaaggga gccatttatg agcagtcttt tagcgctggc gaaggacttg gagcagcaat
     2881 cgaaagcgca gcagcagagt accggcgaga tgctgaaagc cgcattcagc gagcacgaga
     2941 aatctgtaaa agcggaactg agcgcaagcg cgaagagaat cagcgacgcc atcagcgccc
     3001 acgagcaggg tatgaccgcg gcgatgcagt cgaaccgcct gagcgtgctg cgtatggtcg
     3061 gccggacgtg gctgaccatc accctggtct cggtactgct gatcgccatg agcgggagca
     3121 ttctgtggtg gcaggggcag cagatcaccg ggaattacca gactatccgg gcgcaggagc
     3181 ggacgcaggc catgttgtcg gagaaaaacc acggcgttca gctgtcgctc tgcggggaga
     3241 agaaactgtc ctgcgtcaaa gtgaacgcga aagcgggagc ttacggggag gaagggaact
     3301 ggatggtact ggagaggaaa tagcccatga cggaactgga aaaacagttg ctgaccgcat
     3361 tagagcagct acatcaggac tactcgcaaa ggctggacga gtgggagagc gccttcgcgg
     3421 aatggcagaa gatgtctggt cttatgcaac gggagaacgc ggcgctgaac gagcgcgtca
     3481 cgcgcttgag tcagcaggtg gcgaacttga gcaggcagtt acagcgtttg agccagtagt
     3541 gcagcggcat gaaatggcgg tggccgccga acgggcatac gagcagcgac agcacgaaaa
     3601 agagctggcg gaagcccgtt cccgtcagcg gagctataac ggtccgacgc tgggctgaca
     3661 ggcttcacca gtgacaaacc ccacgaagaa gggctcataa atttcccgct aaatacggtc
     3721 gtgatgaaaa catcacggcg tatacaacgg agggattatg agactgttta acgtgattga
     3781 gacggtgctg aactgcctgt acatggcgct ggagatctgg gactggctgg agaagcattt
     3841 tcctgttttt aaggcgttca tccagattct ggcctgaaat cagaccgtat gcatctggct
     3901 gcatgaccat gcagccgggt aaaatttatc tgtcacgaac ggggcggtgt atcgggtggt
     3961 ttgttgccgg ttttacctga ctgccgcccc gtttgtgacg aacatgtccg gagcggtgcg
     4021 ggcaagagat gttatggtaa atcatattag tt
//
