LOCUS       NZ_CP125123             5010 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 189 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP125123
VERSION     NZ_CP125123.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790402
            Assembly: GCF_037477845.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477845.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:31:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,719
            CDSs (total)                      :: 5,595
            Genes (coding)                    :: 5,455
            CDSs (with protein)               :: 5,455
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 54 of 140
            Pseudo Genes (incomplete)         :: 87 of 140
            Pseudo Genes (internal stop)      :: 19 of 140
            Pseudo Genes (multiple problems)  :: 17 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125123.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="189"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(join(4592..5010,1..196))
                     /locus_tag="P4G34_RS28515"
                     /old_locus_tag="P4G34_28480"
     CDS             complement(join(4592..5010,1..196))
                     /locus_tag="P4G34_RS28515"
                     /old_locus_tag="P4G34_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_019725132.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            598..999
                     /locus_tag="P4G34_RS28520"
                     /old_locus_tag="P4G34_28485"
     CDS             598..999
                     /locus_tag="P4G34_RS28520"
                     /old_locus_tag="P4G34_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_019725133.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1799..1960)
                     /locus_tag="P4G34_RS28525"
                     /old_locus_tag="P4G34_28490"
     CDS             complement(1799..1960)
                     /locus_tag="P4G34_RS28525"
                     /old_locus_tag="P4G34_28490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            2197..2442
                     /locus_tag="P4G34_RS28530"
                     /old_locus_tag="P4G34_28495"
     CDS             2197..2442
                     /locus_tag="P4G34_RS28530"
                     /old_locus_tag="P4G34_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            2423..2698
                     /locus_tag="P4G34_RS28535"
                     /old_locus_tag="P4G34_28500"
     CDS             2423..2698
                     /locus_tag="P4G34_RS28535"
                     /old_locus_tag="P4G34_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            2946..3851
                     /locus_tag="P4G34_RS28540"
                     /old_locus_tag="P4G34_28505"
     CDS             2946..3851
                     /locus_tag="P4G34_RS28540"
                     /old_locus_tag="P4G34_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_019725130.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replication initiation protein"
     gene            3848..4555
                     /locus_tag="P4G34_RS28545"
                     /old_locus_tag="P4G34_28510"
     CDS             3848..4555
                     /locus_tag="P4G34_RS28545"
                     /old_locus_tag="P4G34_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004198042.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
ORIGIN
        1 gaactgatgt atctctaaga tattcatctc ttattatctc tcttattttt tgtggggctt
       61 catcttcatc tatatcacct aatgatactg agcgattcac gaatatttca tgttcctcat
      121 tcattttttc aagtgcgttt ttgtagtact gatcattcgc atggtggtag ctaatgaata
      181 ccttatgctt tcccattttt attgtccttt ttttgtttgt gtgcactcta ttttgcactt
      241 gaacctatat cagataacag ctctctttta gagagctctt tttttctcag ccagcacaca
      301 tagggtccct tcagcaaggt ctcgccatga ctggtttagg gcgggctcgt tgcttcccta
      361 aacgccttga gaagagagct atcacctcac tttattctta cgtaccgctc gccgcagtct
      421 tatgaccgaa cgtagtgagc gaatgcgcga ggaagcggaa agtcatttgt tgcctgcatt
      481 ttgcacggtc actcctgtgt ttcatcgccc ttctgaaaaa cacatcactt ctggaccagt
      541 atgtcgtaag ctggcccctg tttccttgag actgaatgta ttaaaggatg ataactgatg
      601 accagagtgt ctattagtga ggctgcacgg ctgacgggta agagccggac aacgctgcac
      661 aggttaatca aaacaggtga actgtccact tgtagcggtg aacgaaatac aaaaatggtc
      721 gatatatcag agcttttacg tgttttcgga ccgtttgacc aaccgaagaa tgaacacctt
      781 actcgacaag tacctgaaca gcgtgataca gcgcctgctg accagagtga acatgtcatt
      841 cgtcaactga aacagcagat tgaacacctt aacatattga ttttatcgaa ggattcgcac
      901 attgacagcc tgaaacaggc catgctcctt cttgaaaatc accaggcaaa attaactgaa
      961 cccccggcgc cgtggtggaa attctggaaa aaatcctgac cccgtcatgc ttcagttttc
     1021 ctctccggcc attccgggga gttcctgaac cttcccgaaa atcgtcagat ctctgaacgc
     1081 tcctcaccac gaaaaatacc cacaccccac gcaaaatttt aattttgcag atttgtattt
     1141 aatatcagca gcttacttta ctgaaatttc gttttttaaa ttttatttat atagatccga
     1201 ctctatgacg agtcgtcata gagtccccgg cgagtcgtca tagagtcggt aacgactcag
     1261 gcctataaaa acaatgagtt aataacgacc cgactctaga tccgactccc cgtttggttg
     1321 ttttttagac gtggcgactc tgggtacgac tctagtgaag agtcgccggg gaatcgtgtg
     1381 gtgagtcgtc cggcgacttg ccataacgtg attgccggcc gtggacgaac cgattcctga
     1441 acgtgctgaa aaccggtttt ccggctgcgc cggcaacgct tcacggccac gcaataaatt
     1501 gcgtccccgt tcaccgtttt cggatgggaa gaacgccgga aacaggactt cccctggctg
     1561 aatggtgtca atttccgcgc tatgcacttg tgcgcatact catgcatgcc gtaaaaacag
     1621 agcctgcgcg tttctggcgg gttttcgggt ggtttgttgc cttgtttacc ggtttcccgt
     1681 cagaaacgcc ctgagggcat ctcaggcggt gctgataacg gatgttatgg taaatcaagt
     1741 gaatggcagg tacaaaaaaa gccccagagg gcttttagtt tagacttctg aagatatttt
     1801 atttttcttg gaagtggtgg ttgtttttgc tcggagggaa cttatcaccc ttgctcacat
     1861 ggcgatcata ttcagaacca gcgtatgagt tgtcggtatc atgatattga ccgtaagtac
     1921 ctgtttttgg acattcttgc ccagaccaat gttttgccat ttacttttcc tttgctcgtt
     1981 ttaggagcta agctcctagc ggtacgaaat tgtgccgcac ttcataaatg taattgcttg
     2041 aaattatgct tcaatgtatt gacttgttgt ttttgtggtt attcgattga gttcacattt
     2101 tgagctgatt tggttgtggg tttttaggga aatatcttta tgtacttatt tgcgtacatg
     2161 ttctatgcta agccccaaca aatgggggtt aacaacatgc atgcaatcaa ttttactgat
     2221 gcccgtaaac attttgccga gacgatgaag cgcgtcactg atgatgctga accggtacgt
     2281 gtcatgcgcc gtgatgcgcc tgatattgtt atgatcgatg cgggagagta tgaggcgcta
     2341 atcgaaacgg tctatctgtt cagcaatccg gcgaacgcag agcatatcaa tgagtcaatg
     2401 aaacaggcgg agcgcggcga atttgtcgaa gtggaatatt aagttcacta ggaatggcgc
     2461 tgatgacctt aactactggc gtgatactga caaaaagaag tttgagcgta tccggcaact
     2521 gttgctcagc atcgaggctg atccgctgac aggtatagga aaacccgaac ggctgcggca
     2581 ccacaaagat ccggcactct actcccgcag gattgatcag agccaccgtc tggtctattc
     2641 aatccagaac ggagaaatca tcatctacgc agcccgttat cactatggtg acaaatagca
     2701 aaagtcgata tagcccgctt gatgcgggct tttcatgcaa ggcgtacaac ggatgttagg
     2761 gtaagtcgct gtcagatcga cacaaaggca aagtcggtgt aacgttagcc tcagaaaaaa
     2821 caaagccccg gaaatcatgt catatcttgg cggatcgcat gataaccagg gctaggtcga
     2881 aaccttgaaa ggatattagc acatgaaccg tgccagacaa cagcccgcca taagcgcagg
     2941 gcacattgca taacgccgca ctcgaacttt tcaacgaacg cttaccacac aagccgtact
     3001 tttcggacga tctgcacttt ggtgtccgta tcgccggtaa ggaacgcgct attctggcga
     3061 aatatatcca gttcaatcag cctcacgcca tgttctggct gggatttgac gttgaccgcg
     3121 ccggttcctc catcgactgg agcgaccgca acgcgcctgc accgacgctg accataacta
     3181 accccgaaaa cggacacgcg cacctgctgt acgcgctgga aaactcgata cgcaccgcgc
     3241 cggacggcaa aataaaaccg ttacgctacg ccgcctccgt ggagaatgcg ctacgtaaaa
     3301 aattagaggc tgacgctgga tattcggggc tcatctgcaa aaatccgaac cacaaccact
     3361 ggaaaatcgc cgtgtggcag ccgaagctct acactctgga ctggctggcc gactttctcg
     3421 atctgaacac agggaatgac aaggaaatcg ttgccgacta cggcttaggc cgtaactgca
     3481 cgttgttcga caaaacccgt aagtgggcat atcgcgccat ccgtcagggc tggcctgagt
     3541 atgagcagtg gctgcaagcc tgctacgaac gcgcttgcgc atacaacctg caatttgcac
     3601 ggcctctgga tgaaaatgag gtaagaggga tagctaagag cattgctaaa tggacatcaa
     3661 ataaattttc acctgaagaa ttcagtaaat ttgttgatat tactcattcc tctgaaattc
     3721 aatcaaagag agggaaaaag tcaggacagt caaggaggaa aggaagccta gaggaaataa
     3781 aaccttgggt agctatggga ataagcaggc ggaagtattt ttatatcaag aaaaatggtg
     3841 aaataagatg aaaaccttcg taactctttg gactatagtt tttttagcat ttattgccgt
     3901 gatgtgtact gttgatatat ctaattacat tccgtctgtt tacacagttt ttagtaagaa
     3961 tagacctctt gtaactggcg ccctttctat tcttttgata tctgttttta tttggctgat
     4021 ggtatccctc tacttactta aaaagtattc ggttaaggtt gagaaactta gccttggtgg
     4081 agtgaatata cttttcaatg agagtgctac actttacaaa aagagtgtaa aaaatcacct
     4141 agattcaaaa agagcaattt tcaaattaag aaaaaacgtt gatgcctttg atgaggttat
     4201 ttcatcatat tatcagactt accaatttat tcgtgatgaa atgaaaatac taaaccctaa
     4261 aaaagatcgt gagatttatg atttcagcaa tgagatgctc atggtgttaa atagatttct
     4321 aactaactac cagaacaact acaagcgttg gtataagtac atatcagaca aagatgaggt
     4381 tgttgatagg gttagcggtg atgtcatgag atttcatgca accagcataa ataaaataca
     4441 aatgcagtat tatgattatg agcgtatttt aaaaggtttt aaagatgtta accggttctt
     4501 tgttaatgat gtgaagccta agtttggaat caatacagat aagtggaact ggtaaattaa
     4561 aggagtagcc aaggctactc cttggatagt actatgaatt atgcattctc atttctctcg
     4621 acatatcata cgttgctttt tctctatcgt tataagtcat gtctattaat tttctgagtg
     4681 tttctgggtt gttttctatt tgattccagt tgaccactga tatataagac ccgctcttca
     4741 tgaagttgtc tattattctt gctggcatat atgggaacct ttcctcataa gttgagcgct
     4801 tatcaataga aaaccattca gttacggtag gatgaacttc tgatttttca ttagttccat
     4861 gtgttgaacg aatgtacgta gtatttgttg acgggagatt gacgactaaa attcctgatt
     4921 ttttgttaat ggtactatct ctcatgctag agtaaagttc ccaatctacg tgttttctgt
     4981 tttttgtttc agttccaaca agaaggataa
//
