LOCUS       NZ_CP125118             5010 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 192 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP125118
VERSION     NZ_CP125118.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790403
            Assembly: GCF_037477735.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 85x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477735.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:26:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,551
            CDSs (total)                      :: 5,426
            Genes (coding)                    :: 5,301
            CDSs (with protein)               :: 5,301
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 43 of 125
            Pseudo Genes (incomplete)         :: 73 of 125
            Pseudo Genes (internal stop)      :: 24 of 125
            Pseudo Genes (multiple problems)  :: 13 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125118.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="192"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(589..990)
                     /locus_tag="P4G28_RS27675"
                     /old_locus_tag="P4G28_27650"
     CDS             complement(589..990)
                     /locus_tag="P4G28_RS27675"
                     /old_locus_tag="P4G28_27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_019725133.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1392..2006
                     /locus_tag="P4G28_RS27680"
                     /old_locus_tag="P4G28_27655"
     CDS             1392..2006
                     /locus_tag="P4G28_RS27680"
                     /old_locus_tag="P4G28_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_019725132.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            complement(2043..2750)
                     /locus_tag="P4G28_RS27685"
                     /old_locus_tag="P4G28_27660"
     CDS             complement(2043..2750)
                     /locus_tag="P4G28_RS27685"
                     /old_locus_tag="P4G28_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004198042.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(2747..3652)
                     /locus_tag="P4G28_RS27690"
                     /old_locus_tag="P4G28_27665"
     CDS             complement(2747..3652)
                     /locus_tag="P4G28_RS27690"
                     /old_locus_tag="P4G28_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_019725130.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replication initiation protein"
     gene            complement(3900..4175)
                     /locus_tag="P4G28_RS27695"
                     /old_locus_tag="P4G28_27670"
     CDS             complement(3900..4175)
                     /locus_tag="P4G28_RS27695"
                     /old_locus_tag="P4G28_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(4156..4401)
                     /locus_tag="P4G28_RS27700"
                     /old_locus_tag="P4G28_27675"
     CDS             complement(4156..4401)
                     /locus_tag="P4G28_RS27700"
                     /old_locus_tag="P4G28_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            4638..4799
                     /locus_tag="P4G28_RS27705"
                     /old_locus_tag="P4G28_27680"
     CDS             4638..4799
                     /locus_tag="P4G28_RS27705"
                     /old_locus_tag="P4G28_27680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
ORIGIN
        1 gtgcatagcg cggaaattga caccattcag ccaggggaag tcctgtttcc ggcgttcttc
       61 ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg
      121 cagccggaaa accggttttc agcacgttca ggaatcggtt cgtccacggc cggcaatcac
      181 gttatggcaa gtcgccggac gactcaccac acgattcccc ggcgactctt cactagagtc
      241 gtacccagag tcgccacgtc taaaaaacaa ccaaacgggg agtcggatct agagtcgggt
      301 cgttattaac tcattgtttt tataggcctg agtcgttacc gactctatga cgactcgccg
      361 gggactctat gacgactcgt catagagtcg gatctatata aataaaattt aaaaaacgaa
      421 atttcagtaa agtaagctgc tgatattaaa tacaaatctg caaaattaaa attttgcgtg
      481 gggtgtgggt atttttcgtg gtgaggagcg ttcagagatc tgacgatttt cgggaaggtt
      541 caggaactcc ccggaatggc cggagaggaa aactgaagca tgacggggtc aggatttttt
      601 ccagaatttc caccacggcg ccgggggttc agttaatttt gcctggtgat tttcaagaag
      661 gagcatggcc tgtttcaggc tgtcaatgtg cgaatccttc gataaaatca atatgttaag
      721 gtgttcaatc tgctgtttca gttgacgaat gacatgttca ctctggtcag caggcgctgt
      781 atcacgctgt tcaggtactt gtcgagtaag gtgttcattc ttcggttggt caaacggtcc
      841 gaaaacacgt aaaagctctg atatatcgac catttttgta tttcgttcac cgctacaagt
      901 ggacagttca cctgttttga ttaacctgtg cagcgttgtc cggctcttac ccgtcagccg
      961 tgcagcctca ctaatagaca ctctggtcat cagttatcat cctttaatac attcagtctc
     1021 aaggaaacag gggccagctt acgacatact ggtccagaag tgatgtgttt ttcagaaggg
     1081 cgatgaaaca caggagtgac cgtgcaaaat gcaggcaaca aatgactttc cgcttcctcg
     1141 cgcattcgct cactacgttc ggtcataaga ctgcggcgag cggtacgtaa gaataaagtg
     1201 aggtgatagc tctcttctca aggcgtttag ggaagcaacg agcccgccct aaaccagtca
     1261 tggcgagacc ttgctgaagg gaccctatgt gtgctggctg agaaaaaaag agctctctaa
     1321 aagagagctg ttatctgata taggttcaag tgcaaaatag agtgcacaca aacaaaaaaa
     1381 ggacaataaa aatgggaaag cataaggtat tcattagcta ccaccatgcg aatgatcagt
     1441 actacaaaaa cgcacttgaa aaaatgaatg aggaacatga aatattcgtg aatcgctcag
     1501 tatcattagg tgatatagat gaagatgaag ccccacaaaa aataagagag ataataagag
     1561 atgaatatct tagagataca tcagttctta tccttcttgt tggaactgaa acaaaaaaca
     1621 gaaaacacgt agattgggaa ctttactcta gcatgagaga tagtaccatt aacaaaaaat
     1681 caggaatttt agtcgtcaat ctcccgtcaa caaatactac gtacattcgt tcaacacatg
     1741 gaactaatga aaaatcagaa gttcatccta ccgtaactga atggttttct attgataagc
     1801 gctcaactta tgaggaaagg ttcccatata tgccagcaag aataatagac aacttcatga
     1861 agagcgggtc ttatatatca gtggtcaact ggaatcaaat agaaaacaac ccagaaacac
     1921 tcagaaaatt aatagacatg acttataacg atagagaaaa agcaacgtat gatatgtcga
     1981 gagaaatgag aatgcataat tcatagtact atccaaggag tagccttggc tactccttta
     2041 atttaccagt tccacttatc tgtattgatt ccaaacttag gcttcacatc attaacaaag
     2101 aaccggttaa catctttaaa accttttaaa atacgctcat aatcataata ctgcatttgt
     2161 attttattta tgctggttgc atgaaatctc atgacatcac cgctaaccct atcaacaacc
     2221 tcatctttgt ctgatatgta cttataccaa cgcttgtagt tgttctggta gttagttaga
     2281 aatctattta acaccatgag catctcattg ctgaaatcat aaatctcacg atctttttta
     2341 gggtttagta ttttcatttc atcacgaata aattggtaag tctgataata tgatgaaata
     2401 acctcatcaa aggcatcaac gttttttctt aatttgaaaa ttgctctttt tgaatctagg
     2461 tgatttttta cactcttttt gtaaagtgta gcactctcat tgaaaagtat attcactcca
     2521 ccaaggctaa gtttctcaac cttaaccgaa tactttttaa gtaagtagag ggataccatc
     2581 agccaaataa aaacagatat caaaagaata gaaagggcgc cagttacaag aggtctattc
     2641 ttactaaaaa ctgtgtaaac agacggaatg taattagata tatcaacagt acacatcacg
     2701 gcaataaatg ctaaaaaaac tatagtccaa agagttacga aggttttcat cttatttcac
     2761 catttttctt gatataaaaa tacttccgcc tgcttattcc catagctacc caaggtttta
     2821 tttcctctag gcttcctttc ctccttgact gtcctgactt tttccctctc tttgattgaa
     2881 tttcagagga atgagtaata tcaacaaatt tactgaattc ttcaggtgaa aatttatttg
     2941 atgtccattt agcaatgctc ttagctatcc ctcttacctc attttcatcc agaggccgtg
     3001 caaattgcag gttgtatgcg caagcgcgtt cgtagcaggc ttgcagccac tgctcatact
     3061 caggccagcc ctgacggatg gcgcgatatg cccacttacg ggttttgtcg aacaacgtgc
     3121 agttacggcc taagccgtag tcggcaacga tttccttgtc attccctgtg ttcagatcga
     3181 gaaagtcggc cagccagtcc agagtgtaga gcttcggctg ccacacggcg attttccagt
     3241 ggttgtggtt cggatttttg cagatgagcc ccgaatatcc agcgtcagcc tctaattttt
     3301 tacgtagcgc attctccacg gaggcggcgt agcgtaacgg ttttattttg ccgtccggcg
     3361 cggtgcgtat cgagttttcc agcgcgtaca gcaggtgcgc gtgtccgttt tcggggttag
     3421 ttatggtcag cgtcggtgca ggcgcgttgc ggtcgctcca gtcgatggag gaaccggcgc
     3481 ggtcaacgtc aaatcccagc cagaacatgg cgtgaggctg attgaactgg atatatttcg
     3541 ccagaatagc gcgttcctta ccggcgatac ggacaccaaa gtgcagatcg tccgaaaagt
     3601 acggcttgtg tggtaagcgt tcgttgaaaa gttcgagtgc ggcgttatgc aatgtgccct
     3661 gcgcttatgg cgggctgttg tctggcacgg ttcatgtgct aatatccttt caaggtttcg
     3721 acctagccct ggttatcatg cgatccgcca agatatgaca tgatttccgg ggctttgttt
     3781 tttctgaggc taacgttaca ccgactttgc ctttgtgtcg atctgacagc gacttaccct
     3841 aacatccgtt gtacgccttg catgaaaagc ccgcatcaag cgggctatat cgacttttgc
     3901 tatttgtcac catagtgata acgggctgcg tagatgatga tttctccgtt ctggattgaa
     3961 tagaccagac ggtggctctg atcaatcctg cgggagtaga gtgccggatc tttgtggtgc
     4021 cgcagccgtt cgggttttcc tatacctgtc agcggatcag cctcgatgct gagcaacagt
     4081 tgccggatac gctcaaactt ctttttgtca gtatcacgcc agtagttaag gtcatcagcg
     4141 ccattcctag tgaacttaat attccacttc gacaaattcg ccgcgctccg cctgtttcat
     4201 tgactcattg atatgctctg cgttcgccgg attgctgaac agatagaccg tttcgattag
     4261 cgcctcatac tctcccgcat cgatcataac aatatcaggc gcatcacggc gcatgacacg
     4321 taccggttca gcatcatcag tgacgcgctt catcgtctcg gcaaaatgtt tacgggcatc
     4381 agtaaaattg attgcatgca tgttgttaac ccccatttgt tggggcttag catagaacat
     4441 gtacgcaaat aagtacataa agatatttcc ctaaaaaccc acaaccaaat cagctcaaaa
     4501 tgtgaactca atcgaataac cacaaaaaca acaagtcaat acattgaagc ataatttcaa
     4561 gcaattacat ttatgaagtg cggcacaatt tcgtaccgct aggagcttag ctcctaaaac
     4621 gagcaaagga aaagtaaatg gcaaaacatt ggtctgggca agaatgtcca aaaacaggta
     4681 cttacggtca atatcatgat accgacaact catacgctgg ttctgaatat gatcgccatg
     4741 tgagcaaggg tgataagttc cctccgagca aaaacaacca ccacttccaa gaaaaataaa
     4801 atatcttcag aagtctaaac taaaagccct ctggggcttt ttttgtacct gccattcact
     4861 tgatttacca taacatccgt tatcagcacc gcctgagatg ccctcagggc gtttctgacg
     4921 ggaaaccggt aaacaaggca acaaaccacc cgaaaacccg ccagaaacgc gcaggctctg
     4981 tttttacggc atgcatgagt atgcgcacaa
//
