LOCUS       NZ_CP125105             4167 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 303 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP125105
VERSION     NZ_CP125105.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33790404
            Assembly: GCF_037477745.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of mathematical biology and
            bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 63x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037477745.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:29:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,951
            CDSs (total)                      :: 5,827
            Genes (coding)                    :: 5,672
            CDSs (with protein)               :: 5,672
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 64 of 155
            Pseudo Genes (incomplete)         :: 96 of 155
            Pseudo Genes (internal stop)      :: 23 of 155
            Pseudo Genes (multiple problems)  :: 24 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125105.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="303"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            complement(join(3968..4167,1..916))
                     /locus_tag="P4G18_RS29725"
                     /old_locus_tag="P4G18_29685"
     CDS             complement(join(3968..4167,1..916))
                     /locus_tag="P4G18_RS29725"
                     /old_locus_tag="P4G18_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(1261..1374)
                     /locus_tag="P4G18_RS29730"
                     /old_locus_tag="P4G18_29690"
                     /pseudo=""
     CDS             complement(1261..1374)
                     /locus_tag="P4G18_RS29730"
                     /old_locus_tag="P4G18_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1790..1981
                     /locus_tag="P4G18_RS29735"
                     /old_locus_tag="P4G18_29695"
     CDS             1790..1981
                     /locus_tag="P4G18_RS29735"
                     /old_locus_tag="P4G18_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1981..2382
                     /locus_tag="P4G18_RS29740"
                     /old_locus_tag="P4G18_29700"
     CDS             1981..2382
                     /locus_tag="P4G18_RS29740"
                     /old_locus_tag="P4G18_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2728..2970)
                     /locus_tag="P4G18_RS29745"
                     /old_locus_tag="P4G18_29705"
     CDS             complement(2728..2970)
                     /locus_tag="P4G18_RS29745"
                     /old_locus_tag="P4G18_29705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(3336..3968)
                     /locus_tag="P4G18_RS29750"
                     /old_locus_tag="P4G18_29710"
     CDS             complement(3336..3968)
                     /locus_tag="P4G18_RS29750"
                     /old_locus_tag="P4G18_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     Typing          complement(1262..1669)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 cagatgaaat taatgatttt attatgtctt taattactga gtaatgtatt ccgttctcga
       61 tttcatcaat aagaataatt gagttttcct gtaccagcac tgagctgata aaagataatg
      121 ctcgactgat accctcgcca agcattgata tttcatttag ctcactaaat ccaatgtcta
      181 aatatatttc cttattgtta cctatcgcag atattgctat atctacaatt ctgtcatcta
      241 ttttttttaa gttttctagt atgtcttttt tccttttttg tgttagtagg ctagatacat
      301 ttgtaattgt tgcctctttg tttatctttc tggatgttgt tattattgtt gctgtcttaa
      361 atttataatt aatcccatcc ctttcgtttt ttatttcgga ttttatttct acgccatctt
      421 ctttcaattg catagagaaa agcttattgg aaaccctgtt tttcgcattg ggtgtctcgg
      481 tcacatttat ttttaaagag tttactgtgg cctgagaacc gatagtttga cgaggtatgt
      541 tttggttaga tagtacggtg tcaacgttta gagacaaact tgattctaat ctctgatttg
      601 tttctgtttc atatgtttgt gttacttcag agcgttcatc tcgaatgact atggacatct
      661 tttgtgatgt gtccatatca tgaaagtaac taacccaaaa tggcttgttt ttgtttattt
      721 ctgccatttc ttttctgaat gcttgaatgt ttaacaaaac agccgggttt tttacatcat
      781 aaaaacaaaa aatagcatcc agtatagatg ttttcccgta gttgtttttc ccagctatga
      841 tatttaaagt ctttaattgc tttagatata gctcattgaa gcatttgaag ttctttaaac
      901 ttatttcctt tatcatgata gcctctgttc gtgtttttgt tatatgccat acgatttgtt
      961 agtaccttaa taaaatgtac agctttcatt gtgattagct agactttatg tcaaagatta
     1021 actttgattg cttgtgtttt aatcgatttt ttttactttc ttcttgagat tatctggcag
     1081 cagccgtaag cttccggctg tgaacgaaaa aaaccgcctt ggcgggcggt tttttcgaaa
     1141 ggttcagtag agttggcgct ctatgaaccg aggtaactgg cttaaaggga gcgcagtcac
     1201 caaaactgtt cgtttagttt agctttaact ggcgtataac ttcaagacta actccctaaa
     1261 tcagttacca gtggctgctg ccagtggcgc tttgtcgtgt ctttccgggt tggactcaag
     1321 acgatagtta ccggattagg cgcagcagtc ggactgaacg gggggttcgt gcatacagtc
     1381 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg
     1441 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     1501 agccatcagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     1561 ttgagcgtca gattccgtga tgctcgtcag gggggcggag cctatggaaa aacggccgct
     1621 gtgcggcctt tccttctttc ctccatgcga ttactcggtt ttctcgagcg tttctacgca
     1681 tgctcctgcc gctcgccgca gccgaacggc aggagcgtag cgactgagtg agcgaggaag
     1741 cggaaaagag gagatcccta cactgaccac tgacgctctg aggcctctta tgacgaaaca
     1801 acagaaaaca gcgctgaaca tggctaaatt catccagaat cagtctctgc tcctgctgga
     1861 gaagcttaac gagcttgatc tcgatgtcga ggccgacctt tgtgagaaac tccacgatga
     1921 tgcggagcat cttttccgta cactgtcctc gagactggat gcattacagg atggtaactg
     1981 atggccagag tgtctattag tgaggctgcc cgactgacgg gtaagagtag aacaacactg
     2041 cacaggttaa ttaaagcagg tgacctgtcc acctgtagcg gtgagcgcaa tgcaaaaatg
     2101 gtagatactt cagagctttt acgtgtcttt ggcccctttg agcaaccgaa gggtgaacac
     2161 cattctggac aggtctctgg acagcgtgat acagcctctg ctgaccagag tgaacaggtg
     2221 atccgtcatc tgaaacagga gattgaacac ctgaacacct tacttttgtc caaggattcg
     2281 cacattgaca gcctgaaaca ggccatgctc cttcttgaaa atcaccaggc aaaattaact
     2341 gaatccccgg cgccatggtg gaaattctgg aaaaaatcct gaccccgtca tgcttcagtt
     2401 ttcctctccg gccatcccag ggagttcctg aaccttccca aaaatcgtca gatctctgaa
     2461 cgctcctcac cactaaaaat acccacaccc cacgcaaaat taaaattttg cagattcctt
     2521 atttacaaca atgggttaat gaaaaataaa tgtgcggtga tgtgttacaa agtgcagatc
     2581 caagtcgtgg acgactctat ggcgaatcgc tgacgaattg cccacgattc atatgtgaat
     2641 cacgctttat tatcatattg ttatgatgaa tcgactcttg attcaattca tcactttggg
     2701 tgttttttgc tcatggtgac tcttgattca attcacgatt cgaatcgccg gtgaatcgtg
     2761 tggtgagtcg tccggcgact tgccataacg tgattgccgg ccgtcgacga acccgattcc
     2821 tgaaggtgct gaaaaccggt tttccggctg cgccggcaac gcttcacggc cacgcaataa
     2881 attgcgtccc cgttcaccgt tttcggagga gaagaacgcc ggaaacagga cttcccctgg
     2941 ctgaatggtg tgaatttccg cgctatgcac ttgcgcgcat actcatgcat gccgtaaaaa
     3001 cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc
     3061 cgtcagaaac gtcctgagtc cgttttagcg gtgcgtacaa ttatgatttt atgttaaatt
     3121 gttttaagtg aatagagaga atataactta agggagcatc ttgtgctggg tcttttacat
     3181 tagatctgca caatcattgt tttggcatct ggtgatgtct ttaggacgtt tgagattgct
     3241 gataactaaa gtgtatgatg ctttctatac gttatccggc gtttaataac accctggtga
     3301 taagccagcc cgtgaagggc tggcttgatt tggatttata tttttgaaat aaaatctttt
     3361 atttcatcaa agcaattgtc tgctgtgttt attattccat ttttcatgga tattcctatt
     3421 cttgagtcgg attctctatt gcagctaaga tagacttgta ccttagcttt tttttcgttt
     3481 gttggataag tgtactcttt gtcactcgct gagatgtcat ctttattatt tttaattttt
     3541 tcgaaaaatt ctattatgta ttcatataac gctcgagtgc ttgcgtgttc aattatcaga
     3601 tcttcaatgg caccttgctt gtcttttcct ggcattataa agaaagaaat ttttgtattg
     3661 ccattcatag ctatctcgtt atgcgagctg ggggaggaga tttgtagttc tcgcaatgtg
     3721 gcaattattg atctttctgt gtcagaaaat gatgtgtcag catctaatat aataaaaata
     3781 ctttcttttt catcgaaaga atcaatgttt ttgaatgctc taagtgcttg ttttaatttt
     3841 gattttccat ttattttttc gacaaaaaca tcggtaatgc cttgtatttc aagtaggttg
     3901 catagaaaat gaaaatcatc atcaccctca acgaaaatga tctttttgct attatttatg
     3961 tctttcatta tcttacctcc cagtcgttct caactgagta ggagaaatca tccatattga
     4021 attgtacggc tgttggtttt agggagttct tttctcttcc tagtctaata taagcgatat
     4081 cttcattttt gctatctatt tcgtttattg ctcgtatcac atcttgacta tgagttgttg
     4141 caaatatttg attgttgttt tgttttg
//
