LOCUS       NZ_CP125093             5596 bp    DNA     circular CON 17-MAY-2023
DEFINITION  Klebsiella pneumoniae strain BAK085 plasmid pBAK085e, complete
            sequence.
ACCESSION   NZ_CP125093
VERSION     NZ_CP125093.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34510231
            Assembly: GCF_030020855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Chen,Y. and Gan,Y.-H.
  TITLE     Whole genome sequence of multidrug-resistant Klebsiella pneumoniae
            BAK085 from Singapore
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Chen,Y. and Gan,Y.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2023) Biochemistry, National University of
            Singapore, 8 Medical Drive, Singapore 117596, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2023
            Assembly Method                   :: SPAdes v. v3.15.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 05/16/2023 16:49:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,577
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,339
            CDSs (with protein)               :: 5,339
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 44 of 117
            Pseudo Genes (incomplete)         :: 67 of 117
            Pseudo Genes (internal stop)      :: 16 of 117
            Pseudo Genes (multiple problems)  :: 10 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125093.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BAK085"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pBAK085e"
                     /geo_loc_name="Singapore"
                     /lat_lon="1.2941 N 103.7830 E"
                     /collected_by="National University of Singapore Hospital"
     gene            join(5415..5596,1..328)
                     /locus_tag="QJS47_RS27835"
                     /old_locus_tag="QJS47_27835"
     CDS             join(5415..5596,1..328)
                     /locus_tag="QJS47_RS27835"
                     /old_locus_tag="QJS47_27835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            335..547
                     /locus_tag="QJS47_RS27840"
                     /old_locus_tag="QJS47_27840"
     CDS             335..547
                     /locus_tag="QJS47_RS27840"
                     /old_locus_tag="QJS47_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(923..1051)
                     /locus_tag="QJS47_RS27845"
                     /old_locus_tag="QJS47_27845"
     CDS             complement(923..1051)
                     /locus_tag="QJS47_RS27845"
                     /old_locus_tag="QJS47_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            1214..1438
                     /locus_tag="QJS47_RS27850"
                     /old_locus_tag="QJS47_27850"
     CDS             1214..1438
                     /locus_tag="QJS47_RS27850"
                     /old_locus_tag="QJS47_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1422..1688
                     /locus_tag="QJS47_RS27855"
                     /old_locus_tag="QJS47_27855"
     CDS             1422..1688
                     /locus_tag="QJS47_RS27855"
                     /old_locus_tag="QJS47_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1758..2108)
                     /locus_tag="QJS47_RS27860"
                     /old_locus_tag="QJS47_27860"
     CDS             complement(1758..2108)
                     /locus_tag="QJS47_RS27860"
                     /old_locus_tag="QJS47_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2174..2719)
                     /locus_tag="QJS47_RS27865"
                     /old_locus_tag="QJS47_27865"
     CDS             complement(2174..2719)
                     /locus_tag="QJS47_RS27865"
                     /old_locus_tag="QJS47_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2745..3071)
                     /locus_tag="QJS47_RS27870"
                     /old_locus_tag="QJS47_27870"
     CDS             complement(2745..3071)
                     /locus_tag="QJS47_RS27870"
                     /old_locus_tag="QJS47_27870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4227..4403
                     /locus_tag="QJS47_RS27875"
                     /old_locus_tag="QJS47_27875"
     CDS             4227..4403
                     /locus_tag="QJS47_RS27875"
                     /old_locus_tag="QJS47_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4393..4737
                     /locus_tag="QJS47_RS27880"
                     /old_locus_tag="QJS47_27880"
     CDS             4393..4737
                     /locus_tag="QJS47_RS27880"
                     /old_locus_tag="QJS47_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4919..5164)
                     /locus_tag="QJS47_RS27885"
                     /old_locus_tag="QJS47_27885"
     CDS             complement(4919..5164)
                     /locus_tag="QJS47_RS27885"
                     /old_locus_tag="QJS47_27885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4062..4141
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3479..3876)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2..663
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.548"
                     /coverage="100.0"
                     /evalue="8.57e-117"
     Typing          4728..5596
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="86.0"
                     /evalue="0.0"
ORIGIN
        1 ggccatgcag tcgaaccgcc tgagcgtgat gcgtatggtc gggcggacat ggctgaccat
       61 cacgatggtg tcaggactgc tgttcgccag cctgagcggg gtgttgtggt atcaggggag
      121 cttgatagcg tcaaatctgg ccgaaatcga ccggcagaac gcagcgctgt cgaagctgaa
      181 cgcgaagacc tggggcgtga cgtacctgga agacagcaac ggacgttttc tggtgctgcc
      241 gaaggggacg gccgtagaca ggacgcagag ctggacggtc gggaacgggc ggtcgaagca
      301 gaacgcgctg agactggtga aggagtaagt gccgatgacc gagctggaaa aacagttgct
      361 gagcgcatta gagcagctac agcaggacta ctcgaaaagg ctggacgagt gggagagcgc
      421 cttcggggaa tggcggaaaa tgtcagggct tatgcaacgg gagaacgcgg cgctgagcga
      481 gcgcgtgacg cgcttgagtc agcaggtgga acgcttgagc gggcaactgc agcgtttgag
      541 ccggtagtgc agcgtcatga gatggccgtg gccgcagaac gggcgcacga gcaacggcag
      601 cacgaaaagg aactgacgga agcgcgttcc cggcagcgaa gctatgacgg tccgtcgctg
      661 ggctgaaatg caggaaaaca gggttctcat cgggcattta cgctgaaacc ggatgtcatt
      721 cacctggctg catggctatg cagccaggta aaatttatct gtcgcgaacg gggcggttta
      781 tcgggtggtt tgttgccggt tttacctgac tgccaccccg ttcgcggcga acccgtccgg
      841 ggcggtgcgg gcaacgggtg ttatgtttaa tcagcgtgaa cggcagatac aaaaaagccc
      901 cggagggctt ttaactactg acttattttt ttgaagattt gctgggtttc aatttgtcag
      961 tatttcccgg cgttttttta ttgtcacgct ggcgtttatc aggttcccct gttgaatcgg
     1021 caattcgttt cggacccggc ttaagaccca taatcgttat cctttcaata gttaagagga
     1081 aagccctcaa gctagatata ttgaccgcgt tacggattat caagaggggc taacaagatt
     1141 attttttgtt cttttaggtt gattgtggta ctgttgatat acatgtatag caaatggtgt
     1201 tcgggaggtt tatatgcttg ctatccggtt atctgatgag attgagtccc gtctggactc
     1261 gctggcgaag caaaccggca gaacaaagac gttttatgcg cgggaagcaa tactggcgca
     1321 tctggaagac ctggaggatt attatctttc agcagaaact gctgcacgcg ttcgccgtgg
     1381 tgatgaagca gtgcattcgt ctgaagacgt gaggaagtca cttggtctgg acgattaact
     1441 attccgatcg ggcgctcaaa tcgttacgca agatggacaa acagaacgca cgacggattg
     1501 tggattttat gagtttacgc attgcagttg ctgccgatcc tcgccagtca gggaagccgc
     1561 tcaaaggtga gctgggcgag ttctggcgct atcgggtggg agattatcgc gttctgtgtg
     1621 agatccgaga tgacgagctt gttatccttg ccgccacgat tggacatcgc cgcgaagttt
     1681 acgactgaag cccgctatgc gggctttttt gatgccgaca acaggtatta tggtaaatac
     1741 tcgactaact acgtgttcta ctgtttgtcg gttttagtta accagcaggt tgtcttaacg
     1801 ataatgtagc cgttattttt aactaataag gtcatattgt taacgtcagg cataataaaa
     1861 gacattatgg cctcagagtt atcatcagaa gcagctctct gcattactac ttcgattccc
     1921 ttttctttga atctataagt atccagttgc ctattaaact tagaaagacc tacaaccata
     1981 cttagtttgt tatctttaac agtaagagat gcttcctgag taccattaca tcggtagtaa
     2041 gcggtgtaac cgtcagcaaa agatcgaccg gggaatataa atgctaggaa cgcaattaac
     2101 caatacatag ttgagaatct tggtattaca catttcacta tcctctcttt catttttcgt
     2161 tcaccatact ttgttatctg atttttaatt tttctatttt ataaaccaag tctctaccaa
     2221 aaaggcgaaa attatcctca aataataata attgtttaag aacatccatg aaagcagctg
     2281 cttcaccctc agttatggga gctactctat ataacatata acctgagtta agggtttctt
     2341 ttatactttc ttcaatgctc attataaata ttagtggatt atcatttttc ttcccccatg
     2401 ggtttctaat taatccttcg atgtactcag gtcctttgcg tcctattgcc tgcgcaatat
     2461 tttttatttc atctgcgtga gggctttgtt gtacagcttt gtttatgcct gcttgagtga
     2521 ttttcagaga ctctacaccg tgtatgttgt atgagataaa atgtaataaa ttaagatgca
     2581 atgcaattaa tccatttttg tttacatctg agtatttgga taaaaaacgt aatgaagtgt
     2641 taactctccc ttgaagccta acaattggga gtgctgcctt gtatatttcc ttctccgtta
     2701 aataccgttt aaaaaacata attctccgcc tccgtatagc gtgcctattt tatttcgtcg
     2761 taaaacttag ctctgaaata ttcctttaga accatcatgt cattaataat tgaatctatc
     2821 tcatccattt tttcttggta ttcttttaat ggtgaaggga gcggtaactc aatatttgaa
     2881 aagtgattgg cgagataggg atcaaggttt ctcatccatt caggcttttc gccaatgcgt
     2941 tcttcaactg aaagtgaacc tgataatctc tggttaataa gatcacttag actcattttg
     3001 gttttattcg aaagccgttg taaggattgc tttaatccta tatctagcat gatatttgtt
     3061 ctttctctca ttaccccccc ttgctattgc gttttttgtt gcgcacaaag ctaccattgc
     3121 gcattaagaa acgcaataaa aaaggaggga gggagtggtt tgtacatgat gtgcagtgcg
     3181 ctgagggcat cagtggcggg tgtttttccg tgggatggat ggagctccgg ggataacaga
     3241 actgacgatc atttaaagga tcttcttgag atcctttttt tctgcgcgta atctcttgca
     3301 ctgtaaacga aaaaaccgcc tggggaggcg gtttgatcga aggttaagtc agttggggaa
     3361 ctgcttaacc gggtaactgg gtatacgaga ccgcagatac caaaactgtt ctttcagtgt
     3421 agccgcagtt aggcacccac ttcaagacct ctcaatatct ctctcgtata tatccagtta
     3481 ccaatggctg ctgccagtgg cgtttttgcg tgtccgtccg ggttggactc aagatgatag
     3541 ttaccggaaa gggcgcagca gtcgggctga acggggggtt cgtgcataca gcccagcttg
     3601 gagcgaacga cctaaaccga gccgagatac cagcagcgtg agctatgaga aagcgccacg
     3661 cttcccgaaa gggaaaaagg cggaacaggt atccggtaaa cggcagggtc ggaacaggag
     3721 agcgcacgag ggagccgccg gagagaaatc accggcatct ttaagtcctg tcgggtttcg
     3781 tcctcctctg acttgagcgt ccatttctgt gatgctcgtc aggggaggcg gagcctatgg
     3841 aaaaacggca acgccgcatc cttttctccg gccctttctg atttgagcgc ctttgcttta
     3901 tcccgttatc cgtgagtcgg tcacgcccgc cgcagccgaa cggcaggagc gcagctaccg
     3961 agtgagcgag gaggcgtcat ttcatctgtc cgtgcatggt attgcggacg ctcctgagcc
     4021 acatatcaca cctgccacat gaggcacttt catgaatcgt gctctcatgc caacatagta
     4081 agccagtata cacagcgtaa acgctgtgac tggttcaggg ctgcgccccg aaacccgcta
     4141 acaccactga cgcgctggcg cttgtccaac gccggagcga cccggaaaac ttttccgtgc
     4201 cgtccggcgt ttgcgaagga gggataatgc agaagccgaa gaagaagccg ctcgtcgtaa
     4261 aaaaaagatc ctggcatccg gcagtgatgg caaatctgat gcgtcaggca caggaggaag
     4321 attaccgccg ggagcagatg gcagagcttg agcgttcagc ccggtcacat tttgcggata
     4381 tcggaggaga ggatggcggt taagcgtacc cgttttttag gtatccgggt gaccgatggg
     4441 gagtaccagc agctgctgga gcgctgcaat ggaaggcaac tggcggtgtg gatgcgggaa
     4501 acctgtctcg atacgcgccc ggcgcggtcg ttgcggctgc cgtccatcga tccggttttg
     4561 ctgcgccagc ttgccgggat ggggaacaac ctcaaccaga tcgcccggaa aattaacggc
     4621 ggccagtggt caggcgctga ccgggttcag gtggtggccg cgctgatggc cattgatgcc
     4681 gaactcgagc ggctgcggca tgccgtgctg gaaaaggggg cagacgatga tcgttaagtt
     4741 tcatccgcga gggcgaggcg gcggtgccgg gccggtggat tatctgctgg ggaaagaccg
     4801 ccagcgcgac ggggccatcg tcctgcaggg aaagccggag gaggtccggg agcttatcga
     4861 cgcctcgccc tacgccaaaa agtacacctc cggcgtcctg tcttttgctg aacaggattt
     4921 accgcccggc cagcgtgaaa agctgatggc gagcttcgag cgggttctga tgcccggact
     4981 cgataaagac cagtacagcg tgctgtgggt tgaacaccgg gacaaggggc ggctggaact
     5041 gaattttctg gtaccgaaca cggaactgct gaccggcagg cgtctccagc cgtactacga
     5101 ccgggcagac cgtccgcgca tcgatgcgtg gcagaccgtg gtgaacgggc gactggggct
     5161 gcacgacccg aacgcaccgg agaaccggcg cgcgctggtg acgccatctt cgttacccaa
     5221 aacgaagcag gaagccgcag aggctattac gcggggctta ctggccctcg cctcgtcagg
     5281 ggagcttaaa acgcgtcagg acgtcactga ggcgctggaa agcgcaggtt ttgaggtcgt
     5341 gcgcaccaca aagagcagca tcagcattgc cgacccggac ggagggcgaa acatccgact
     5401 taagggagcc atctatgaac agtcttttaa cgctggcgaa ggacttagag cagaaatcga
     5461 aagcgcagca gcagagtacc ggcgaaatgc tgaaagccgc attcagcgag cacgagaagt
     5521 ctgtcagagc ggaactgagc gaaagcggga agagaatcag cgccgccatc cgcgcccacg
     5581 agcagggtat gagcgc
//
