LOCUS       NZ_CP125092            10078 bp    DNA     circular CON 17-MAY-2023
DEFINITION  Klebsiella pneumoniae strain BAK085 plasmid pBAK085d, complete
            sequence.
ACCESSION   NZ_CP125092
VERSION     NZ_CP125092.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34510231
            Assembly: GCF_030020855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Chen,Y. and Gan,Y.-H.
  TITLE     Whole genome sequence of multidrug-resistant Klebsiella pneumoniae
            BAK085 from Singapore
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   Chen,Y. and Gan,Y.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2023) Biochemistry, National University of
            Singapore, 8 Medical Drive, Singapore 117596, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2023
            Assembly Method                   :: SPAdes v. v3.15.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 05/16/2023 16:49:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,577
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,339
            CDSs (with protein)               :: 5,339
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 44 of 117
            Pseudo Genes (incomplete)         :: 67 of 117
            Pseudo Genes (internal stop)      :: 16 of 117
            Pseudo Genes (multiple problems)  :: 10 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP125092.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BAK085"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pBAK085d"
                     /geo_loc_name="Singapore"
                     /lat_lon="1.2941 N 103.7830 E"
                     /collected_by="National University of Singapore Hospital"
     gene            140..511
                     /gene="tolA"
                     /locus_tag="QJS47_RS27770"
                     /old_locus_tag="QJS47_27770"
     CDS             140..511
                     /gene="tolA"
                     /locus_tag="QJS47_RS27770"
                     /old_locus_tag="QJS47_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            508..726
                     /locus_tag="QJS47_RS27775"
                     /old_locus_tag="QJS47_27775"
     CDS             508..726
                     /locus_tag="QJS47_RS27775"
                     /old_locus_tag="QJS47_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            1068..1346
                     /locus_tag="QJS47_RS27780"
                     /old_locus_tag="QJS47_27780"
     CDS             1068..1346
                     /locus_tag="QJS47_RS27780"
                     /old_locus_tag="QJS47_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1334..1648
                     /locus_tag="QJS47_RS27785"
                     /old_locus_tag="QJS47_27785"
     CDS             1334..1648
                     /locus_tag="QJS47_RS27785"
                     /old_locus_tag="QJS47_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001136729.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1812..2240
                     /locus_tag="QJS47_RS27790"
                     /old_locus_tag="QJS47_27790"
     CDS             1812..2240
                     /locus_tag="QJS47_RS27790"
                     /old_locus_tag="QJS47_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2266..2445
                     /locus_tag="QJS47_RS27795"
                     /old_locus_tag="QJS47_27795"
     CDS             2266..2445
                     /locus_tag="QJS47_RS27795"
                     /old_locus_tag="QJS47_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2473..3003)
                     /locus_tag="QJS47_RS27800"
                     /old_locus_tag="QJS47_27800"
     CDS             complement(2473..3003)
                     /locus_tag="QJS47_RS27800"
                     /old_locus_tag="QJS47_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065521056.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3010..3741)
                     /gene="mobC"
                     /locus_tag="QJS47_RS27805"
                     /old_locus_tag="QJS47_27805"
     CDS             complement(3010..3741)
                     /gene="mobC"
                     /locus_tag="QJS47_RS27805"
                     /old_locus_tag="QJS47_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_032448355.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(3741..5702)
                     /locus_tag="QJS47_RS27810"
                     /old_locus_tag="QJS47_27810"
     CDS             complement(3741..5702)
                     /locus_tag="QJS47_RS27810"
                     /old_locus_tag="QJS47_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_202179206.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYGLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRWWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7186..7335)
                     /locus_tag="QJS47_RS27815"
                     /old_locus_tag="QJS47_27815"
     CDS             complement(7186..7335)
                     /locus_tag="QJS47_RS27815"
                     /old_locus_tag="QJS47_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7420..7677)
                     /locus_tag="QJS47_RS27820"
                     /old_locus_tag="QJS47_27820"
     CDS             complement(7420..7677)
                     /locus_tag="QJS47_RS27820"
                     /old_locus_tag="QJS47_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(7687..9372)
                     /locus_tag="QJS47_RS27825"
                     /old_locus_tag="QJS47_27825"
     CDS             complement(7687..9372)
                     /locus_tag="QJS47_RS27825"
                     /old_locus_tag="QJS47_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9699..9944
                     /locus_tag="QJS47_RS27830"
                     /old_locus_tag="QJS47_27830"
     CDS             9699..9944
                     /locus_tag="QJS47_RS27830"
                     /old_locus_tag="QJS47_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146442.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     BGC             2687..10078
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6504..7083
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.427"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3014..3741)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.74e-141"
ORIGIN
        1 tgcatgccta tgcacgcaac tgcatggcgg gccgtttggt gtaaaaacga tacgataagc
       61 cgtgtttatg tcaatgaacg tggccgtaaa gagtggcaaa tatacatgta atatttttta
      121 ctgacaggtc atcaacccga tgcgtttaat accctttatc ctgctggcat tgtgcagccc
      181 cttactcctg acggcctgta agcattccac cacccagccg ccgaaaattt atactctgga
      241 taagtcatct aaaatcagta gctacgctgc ggccatcaag accgctgttg aaatgcagat
      301 gtttgaacca gaggcctatg cgggaaaaat gtgcaccgtg cggatatcca tacaacgtga
      361 tggcactctg aacagcgcca ttgctgaagg aggagatccg aaactctgta aagcagcaat
      421 atcagccgtt acccacgcca aaattccgcc ggcaccggat gaaaatacct ggcaaatatt
      481 caaaaacgcc cctctggatt tcagaccatg aaagctttta ctctgacagt gctggcggcc
      541 atgttcctca ctccggtaca tgcgatacca ctaaaacccg taaccacact gcagccaacc
      601 tacccggtac aggccgcagc aaaacgcatc gaaggtcagg tgaccgccac gtttgatatt
      661 ctggacgatg gacgagtgga caacgtgtcg cttaccggct gtcacttttt tttcgggaaa
      721 cgctgacggc aatgaggcac tggctatatc ccgcagggca cccggcaatg cgacaggtta
      781 taaattttga attcagtctg aaaaatgacg gcttacagga tccgcgacca gccaccacca
      841 aaaagttcaa tgatgatggt tgccgccagt aacaggtcct ttaacccgca ggctgcaata
      901 gtcctggatg atgagtgagc ctgtagcagc cctgattagt ggaagcgaag ctgaaaaatg
      961 gggaagcgaa gctgaaaaat gggtagcaaa agccgtattt ctggcaaata gcgccgccac
     1021 ggattaaagt agtcatatgt attacacatg actacaaagg ggcttttatg tcatcagcaa
     1081 catccatcag gctcgatgaa gaacttaagg accggctgaa aacgctggcc gatgaccggc
     1141 accgctcagc gcacgcgcta atgctggagg caatcaccga atacatcgac cgggaagaga
     1201 agcgcagcca gtacctgcgg gacggacaag ccgcatggca gcactatcag gaaacgggtc
     1261 tgcaccttac tgcggaagaa gcagaagcct ggataagtac ctggggaact gagaacgagc
     1321 aggatgctcc gccatgccac aggtaacgat ttccgcgctg gcacagcgag atttacaacg
     1381 cctccaggac tttctcaaaa ccaaaaatcg gctggcagcc agaaaagccg gtgaggtgat
     1441 cgtccgggct atccagcaac tgaaaacatt gccagacatt ggccgcccgg tgccatttct
     1501 gccgctggaa tatcaggaac tggtgatcgg gtttggcgac agtggctatg tgatgctcta
     1561 ccgccacgac cgggaaatgg accggattgt gatcgtcacc gtcaggcatc agaaagaatc
     1621 cgggtatccg ggggcagaca gcctctaata accgatgacg ggggcgcaat tcattgcgtg
     1681 gccgtcgccg gtttgacaag cacagcgcgt cagaaaacca catcctgaac ggaaaaacgg
     1741 gtaaaattca gcgatacatt taccgtgaga aattacacat ctttcacacc cgataaaatc
     1801 aggggtatac agtggcgaaa acattgctca acctttcaca ggcagcacaa gccgcgggca
     1861 ttaccagaag aacgctatac aatcatgtaa aacagggaaa ggttaccgtg tcacgtgacg
     1921 ggaaaaacaa ccctgtcgtg gatgtgtcag aactgatccg tgtatacgga aatgtgaata
     1981 tccctgagaa acaaattccc ggcatttcac accgtgaaaa tacacaaaaa aatttcccac
     2041 aggaacagct tctggcaatg caaaaggaac tggccgatct ccggcaggct gtcaccctga
     2101 tgctggaaga taaaacatcc cgagaagaag aacgccgcca gcacgatgat gagcgccgga
     2161 aactccaggc cgaagtggac agattgacaa cagaactgac acagaagaaa aaacgcttct
     2221 ggtccggctg gtttagctga gaacttaccg aagggaatgg agaacatgac gaagcaggaa
     2281 aaaaccgccc tcaatatggc taaattcatt aaggaccagt cgctgctgtt gctcgagaaa
     2341 ctgaacgagc tggatatgga taccccggcc gacatgtgcg agcggctgca cgaggatgcg
     2401 gaaacactct ggcaggtgat gcaggataag ctcggggaag aataacagca ccggcatggc
     2461 cggtgctggc gtttattttt tgccgaacgg ccacctccgg cgggcgggtt tatcctccgg
     2521 cagttctggc accggaacgt taaaatagcg gctgttatat tcccgcatca gctccgcttt
     2581 cagcttctcg ttcaccgcgc tttccccgta ccgctcactc acgacgtcaa tcatcgcccc
     2641 gatctgctcc ggggtgtatt tcccggcgtc cagtacctgc acgccgttct cgatggcgaa
     2701 ctgctcaacc ttgcgataaa gataatcaca cagcttcatt ccggccggtt tctcacgttc
     2761 ccacgccgga tttttgccgg tgaagtaata cgtggctttg tcatcgctgt tcagccgttc
     2821 gtgatacatg ccgataaact tcatcattgc cagatgctgg gcctgatctt caaacctgaa
     2881 tacatatgca ccggtctgcc aggccatgac cgccatgtct tccagatgct cgctggttgg
     2941 cataatgcag cgctcgccgt ctttgtcttc cacgtcctca ataaatacca tgtacagcgc
     3001 cataatccct cactccgttg tcataaattt aaagcatgcg aacagcctct cccggtccag
     3061 ctccgccgtt ttgcgggcct cgccggtctg cgggttcacg atttcatcca ccgccagaat
     3121 cgcccgctgt acccggacaa gcgtcggccg gtcagggcag atgtaccaga tttcatcgat
     3181 gccgttcgcc ttacggttga acagataccc ggacaccact tcctgatacc ggcgacggct
     3241 cttgacggta cgttccacct caaacgccac cgttctgccg tccagctccg ccagcgcgtc
     3301 cggcaccttc atccctttca gggcaatacg gtgcaggtac cgccagccgg tggccccccg
     3361 ggcctccatg gcgaggcgca gggactgcac ggccagccgg tgcggcagct gcgccgccga
     3421 cacccgcgac ggctgaaacg gaataatgtc cgtcagcggc tcgtcctcgt cgaacgagaa
     3481 cgccaccccg tgcggcgtca gcccccacac gctgacctga cggccgtcaa ccgggtattt
     3541 cgcccggacc agcagccctt tcgccaccag gctgtccagg aagcggtaaa ccggctgtac
     3601 cgccgcaatc cccagccgtt gcgcaaccag ctccgccgtg gaacacagtt cttcccgcag
     3661 ccagcgcagg gtcagccgtg ttttctccgc gttccgtgcc tgccgcttcg cgtggctgac
     3721 gttgtatcgc tccagtgcca tcagtacagc cccgcaaaat catcatcacc cggccgctgc
     3781 cgcccggcct ccgactggta ctccgccagc gggtccgttt tcgccagcac agggaaggtt
     3841 ttcagcggaa tttcccggcg atcaacacgc agcgggctgc tgaacgccag ccgcgccacg
     3901 cccagcccgg tcatcaccgc gaacccgtca ggcagatgtt gcagggtgtt cacgtcaaac
     3961 agcggccgcg cctccttctg gatgaagctg tccccgctga tatgctccac gttccccgcc
     4021 tcccggctgg cccggcggcg ctccacgtcc acccggatct ccccggtctg tccggccgcc
     4081 cactgagcgg actcctggct ggccgcccgg tagaaccagc ggatcggcgt gttatccagt
     4141 accgtggttt tcacaaaatc agcgggcaga tcctgcccgc actggccgaa gtcgcccagc
     4201 gactggtgcg cgagcagcag gttgcagtcc cggctgcgca gcgtccccag cgcgttcagc
     4261 acgtacttcg acagcagata tttaatctca tcgagcatga tgctcgcgtg cggccaccac
     4321 cggaactcgt cccgcgcaat gatgatttgc gcgcagcggg caaacagcat cttctgcacc
     4381 cggatcaccg cctcatcgtc catcgagccg atgacgtaca ggcagccgcc gccgttgatt
     4441 atccccgcca cgtcgatgcc gctgtccgtc tgaaggacgc tcaggtcggc cacgttctcc
     4501 agcttggtga tcagcccctt cacgtccttt ttcagggcct ccggcagtgc gtacgccgcc
     4561 gccagcacct gctgaatatt tgccccctgc ggaaactgct ccgcaatcag cttcgccgct
     4621 ttctgctcgc tgatgcggta gtggtccgcc acgtcgccgg tctcactcag gttaaatccg
     4681 gcaaccagaa gattcttcag ggcgtactgg tcgagatcgc ggaacagatt cagctgcgcc
     4741 ggtttccccg gccgcagatc cagcagggtg aagtccgggc agtgcgcccg gaagacgtgc
     4801 ggcgcccacg cgtccccttt cgggtcgaag tacaccaccg cgtccccgaa atcgcgtacg
     4861 cagcgaatga gcgcgttggt ggccatcacg ctcttgccgc tcccgggcag gccgaggatc
     4921 tgaatgttgg tcttgcgcca ggtatcacgc gggaccagca ccggctggcc ctgctcatcc
     4981 ttacccagaa acaccgcctg ctgtttccgg gcctgctccg tggaaaaatc ggccagcgcg
     5041 ttaaccggta ccggctcccc gaaatgcagg tggcggacgt cggtcatttc tccggtccgc
     5101 tggctgctgc gccggctctc ctgattcagt ttcgcgttca ccgcctcgtc agcggccgca
     5161 aaatgcccgt caagcagcag gaacgccagc agccgcgcca ggaggacgcc tgcgacaaga
     5221 cctccggcac accaggcaat cccgaacagg ttagccggct gcagcaggta ataatctgcg
     5281 ttcgcgtaga ggtccggacg caggacggtt tctccccagc tctcgataaa cacggcgaca
     5341 accagccccc ccagaagggc cagaccggca gcgccggtca gcccgtatgc gtagttccag
     5401 acatgtcgcc gcgccctgac catccgcccg ggcgaatcca gcgaggccag caccgaaaat
     5461 ggcgcagtcc ggttattatc gcgcttatag cgatcggcat actcccgccg ctccttatga
     5521 ctcagcccca cgaatcgcat ccgcccggcc cagagttctg ccagaccgaa aaacaggccg
     5581 ataaccagaa aaaaaccggc cagactgtaa tactcccggt acagccagct gacccacggg
     5641 taaatctgcc gggccagctc cgggatatgc cactgccagg caagcgaatc cgtattaaac
     5701 atgcaatctc tctccttcag acgacgggtt taccggcgtg ttgtgcagcc cctgctgcac
     5761 aagccgcgga catgccccaa ggcatggcgg cggcggtcac gtccgtggtg attgcgttca
     5821 gctttcaccg cggaggccag cctctttccg ccccatgcgg aaaaggcgca gccccggagg
     5881 aacaacgagc acgccccagc gtgtgagagg aaaaggcggc accccagtgc ggtcttttcc
     5941 ggtgttccgg cccccgtcag agttcgccat agcgagcggc tgaccacccg caaaatcagg
     6001 agcagctttg ctgcttcgtc aggtgcgtcc agcaccgtgg attttgtggg tggtcaggcg
     6061 tgagcctgac gggggccggg tgaatttctg gcgtgaccgg cgtccagccg gacagagcca
     6121 gaaagagggg gaaagacagt gcgtcggtgg cgtccagcca gcgtaagcag gggatttccc
     6181 cctgaagtca gtcacgcccc agcggggatg acgtgcattt ctccgcaagg agaaaaaagc
     6241 aacaaagata ggttaacgta aaatattcag cagaaaaccg acatacttca cctatctgaa
     6301 taatttatcg gcagggaaga gaaaggcggt aacacggagc aatatgtcac atccacgcgt
     6361 ccggtgaccg tcttgcaggt gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg
     6421 cgccggccgt gagactgcgg cgggcgctgc gaactctcgc aaagttaccc acagattccg
     6481 tggataagca gggaacgagc atgtgaggca aaacggcagg gccgcgccgg tggcgttttt
     6541 ccataggctc cgccctcctg ccagagttca cataaacaga cgctttttcg gtgcatctgt
     6601 gggagccgtg aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa
     6661 gatccccacc gtttccggta ggtcgctccc tcttgcgctc tcctgttccg accctgccgt
     6721 ttaccggata cctgttccgc cgttctccct tgcgggaagt gtggcgcttt ctcatagctc
     6781 acacactggt atctcggccc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa
     6841 ctccccgttc agcccgactg ctgcgcctta tccggtaact ttcatcttga gtccaacccg
     6901 gaaagacacg caaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat
     6961 ttgtttagct aaacacgagg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa
     7021 ggacagattt ggttgctgtg ctctgcgaaa gccagttacc cggttaagca gttccccaac
     7081 tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga
     7141 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgcgat
     7201 ccccgtcagt tcagaggagg acgatggtgc caccgttccg ccctgaacat cccggatata
     7261 atttgcctga catgcggcca gaagaaagcc agcaacaatc aatataaaaa gaaaaatagt
     7321 ctttaccttt ttcatatact ttccctgtat ttaaagactc cggcattcat ccctgaacac
     7381 cggaagtcag tatttcaaat catccttgat aattttttgt taccagttac cgtcccgata
     7441 atcaaaagct atataacaat tatccacatc aggttttatt tgatttttaa aaaatggctg
     7501 tagtaaagga acccattctt tcttcaaatc aaactcacca ttattaatat tatcctttaa
     7561 aggcattccc aagctttcaa tgacagaacc atcgtcacca aagtctttag aatactcttc
     7621 ccctttaaac tcttcagttt tcttatcaaa ccatgtcaaa cgtaatttaa gccccatatt
     7681 tacctctcaa agatattttt taatgttccg ttttggatct ggccctttaa cctgcttacc
     7741 cgttttggga tcaaatgccc cgaggtgttc gccatcactg gcacgatacc cttcaagctc
     7801 accgtgctgg gaatcccatt cataaatttt acgtttttta tcgccatacc atcgggcccg
     7861 cttaccacca ccaccttgtt taggtgtttt aggatcacct cgctttaact ctccaagtcc
     7921 ctttatgtct tctgttttag gtactggatg ataatcatgg ccgtagtctt tagtcccctt
     7981 gcgaggcttg ttcttttcct catttaactt attttctgca tccttcgctt ttttctcctt
     8041 ttgtttccgg ctctccatcg ccccggtaag tgcagcctta tctctggata aggattcatt
     8101 cattgcgttt aaagatgcct gagccgcttt taatttctca ttctgattct gaatatcctt
     8161 atcaagatag cgaacctgat cctgccaccc ggagccatat tcatgaggcg ggttataagt
     8221 cacaaaatca tctttatttg cctctgcctc ttttaccttt ttatcggctt cctgaacagc
     8281 agcccttgca ccggggagcg ccttctctgt cgatgcaatg cggctgttta aggtcgcaat
     8341 atttttatct tcggcatcca gctcagcttt cgctttatca tagtctcttt tcagcccctc
     8401 ttccggatga gcggcgtccc atgcctgctg gcgacgcttt tcttcctcct gacgctgttt
     8461 tacctgtgcc ggggtaagaa catctgtcac cgacacataa accggcttct gtccggtctc
     8521 tttcgggaaa cgaataacgg cctcacggga gttaccaccg gctgtaaaac cagccggacg
     8581 tgaatcacct ttttcagcaa caatgccttt tggcggggct ttcgctgtcg gaacaccttt
     8641 cggtacgctc acctgcagag acgggagacc cggaatcgac acactgaata cccccggacg
     8701 ttttgtcggt ttcgcatcca ccacagggac actcattggc cgacctgcga caaccgcaat
     8761 atgctgccgc tcatccttca ccacatccac aacccgttgt ctgacacgaa ccgtcgcctg
     8821 gtccagtggt aaagtacttg ccggagtctc cgtcaccgca tcggccggta atgacgtcat
     8881 aatttttgac atcattttcg gatcatcttt tgcaatctca gaaggcagca caccgtagat
     8941 cgcaatcccc cacagaccaa acttaaacgg ccccttcagg gcagccagca catcagcaac
     9001 ggcggccgac aacgcatcgc cggaaactga taaagccagt ccttcagccc ccggagtagc
     9061 cagggcaggc agaccgaagg ccatcgcagt ggccgaagac tgtccgccac cagagctacc
     9121 gctatgattg ccgctgttac caccattacc accgggttta ctcccgccgt taccaaactc
     9181 ccccggatta tagttatgaa tatctccatt cggcgtatgc gtcgtacccc agcccggccc
     9241 gctgtttgga tccgtaccac cacctgatga agggccacct ttaccagacg taccgttcac
     9301 attcccactg gcctgaccac cattatgtcc cagacctgaa ttacccggac ctcgaccgtc
     9361 tccaccactc atatcgtttt cctctttgac atttaaaaca ataaattaaa gccaacatac
     9421 tgtacatata cacagtacaa ttattacctt actcctcccg ttttcaggtg tcaatatatg
     9481 agattagttc tcatcttcag gcacaaaaaa gcgcccggag ccacctgtaa gcagatcgcg
     9541 tcttttcccg aaaatttctc acggcaacaa attttattat ttgagatgta catcacactt
     9601 acatttcagg tggtatagac acatctgaaa gctaactggt atgaggggag gacccgcggt
     9661 taaactgttc aggaaacctg agacgcccgg aggccaccat gacacacctg aaactgactc
     9721 ccgctgaaga aaagcgccta gcggacgcct ttgtcgctca ggcactggcc atgggcctga
     9781 aagggaacag aacgctgcca aaggcggtaa aacagcaggt gcgggaaagg gtgcttcgcg
     9841 ccattcagga cgaacgtgaa gccaggcggg cgcaggaaat gcgtcgggag aaatacgagg
     9901 cggcggaaac caccttcacc tggcagccgc tgcgccgccg gtgaggggta tttaccataa
     9961 aacccgttat cagcaccgcc cgagaagccc tcagagcgtt tccggcggct acgggataaa
    10021 acaggcaaca aatcacccgg aaaaccgccg gaaacgcgcc cggtaaattg taacccgc
//
