LOCUS       NZ_CP124903            13517 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Enterococcus faecalis strain EfsC94 plasmid p2_EfsC94_repUS41,
            complete sequence.
ACCESSION   NZ_CP124903
VERSION     NZ_CP124903.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33741206
            Assembly: GCF_030020535.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13517)
  AUTHORS   Sharon,B.M., Arute,A.P., Nguyen,A., Tiwari,S., Bonthu,S.S.R.,
            Hulyalkar,N.V., Neugent,M.L., Dillon,N.A., Zimmern,P.E., Palmer,K.L.
            and De Nisco,N.J.
  TITLE     Functional and genetic adaptation of urinary Enterococcus faecalis
            identified by comparative genomics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13517)
  AUTHORS   Sharon,B.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2023) Biological Sciences, University of Texas at
            Dallas, 800 W Campbell Road, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 439x, 346x
            Sequencing Technology             :: Illumina NextSeq, ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030020535.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 04:52:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,879
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,730
            CDSs (with protein)               :: 2,730
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 34 of 74
            Pseudo Genes (incomplete)         :: 23 of 74
            Pseudo Genes (internal stop)      :: 22 of 74
            Pseudo Genes (multiple problems)  :: 5 of 74
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124903.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13517
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="EfsC94"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="p2_EfsC94_repUS41"
                     /geo_loc_name="USA: Dallas, Texas"
                     /lat_lon="32.77 N 96.79 W"
                     /collection_date="2019-03"
                     /collected_by="UT Southwestern Medical Center/ UT Dallas"
     gene            complement(join(13489..13517,1..523))
                     /locus_tag="QLQ35_RS14245"
                     /old_locus_tag="QLQ35_14245"
     CDS             complement(join(13489..13517,1..523))
                     /locus_tag="QLQ35_RS14245"
                     /old_locus_tag="QLQ35_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_002396187.1"
                     /translation="MSYNVQPLRTQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRMS
                     DIVKLKKKDVISSKNPRIVEQKTGKTRILYLSSLQELIQDYTKDLEPEDYLFPSTKGGH
                     VEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKITKR
                     YIGINEDEISETLLNFRLGF"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(764..1381)
                     /locus_tag="QLQ35_RS14250"
                     /old_locus_tag="QLQ35_14250"
     CDS             complement(764..1381)
                     /locus_tag="QLQ35_RS14250"
                     /old_locus_tag="QLQ35_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="WP_002319351.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGLRQIFWGQYL
                     GTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVALVGEEEEEEEEVVEKLESR
                     GTNRFFWTVALITIASGGDNLGIYIPYFASLSFSEIVTALIVFAISVAVLCYISYKLAK
                     ISFVSETLEKYERILVPIVFIGLGIYILIENGTIQTVLSLFN"
                     /name="CadD family cadmium resistance transporter"
     gene            complement(1398..1760)
                     /locus_tag="QLQ35_RS14255"
                     /old_locus_tag="QLQ35_14255"
     CDS             complement(1398..1760)
                     /locus_tag="QLQ35_RS14255"
                     /old_locus_tag="QLQ35_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_282861583.1"
                     /translation="MEKVCQAAIVHKEKVNVIKNKLDQKDFSNLLVLGKCFSDSSRIKI
                     FYALETYKEMCVCDLAEILNASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDILS
                     AIHIFLKLSENLSMKS"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            2257..2382
                     /locus_tag="QLQ35_RS14260"
                     /old_locus_tag="QLQ35_14260"
     CDS             2257..2382
                     /locus_tag="QLQ35_RS14260"
                     /old_locus_tag="QLQ35_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_229236143.1"
                     /translation="MSRSHLNYDLVDRTYNYKTDIERYINENKSSPRAIRNKDEL"
                     /name="plasmid recombination protein"
     gene            complement(2808..3236)
                     /locus_tag="QLQ35_RS14265"
                     /old_locus_tag="QLQ35_14265"
     CDS             complement(2808..3236)
                     /locus_tag="QLQ35_RS14265"
                     /old_locus_tag="QLQ35_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005917942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="WP_002396183.1"
                     /translation="MSNEIQKRNSDTYNMTPFNFFEDFSRNLFNDFKPNLIKTDIHETD
                     NEYFVEAELPGIPKEDIQVTYENGVLTISGQHQIDTATEDKKGRLIRSERSLTSVRRQY
                     LLENVKEDEIKASYSEGILKLTLPKDSNKEIKKSIPIE"
                     /name="Hsp20/alpha crystallin family protein"
     gene            complement(3336..3590)
                     /locus_tag="QLQ35_RS14270"
                     /old_locus_tag="QLQ35_14270"
     CDS             complement(3336..3590)
                     /locus_tag="QLQ35_RS14270"
                     /old_locus_tag="QLQ35_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epsilon-antitoxin"
                     /protein_id="WP_010824143.1"
                     /translation="MKLDYRKTFEIEIINEFQSAIHSKMLNFVLNNEFDKSDSKNLQTN
                     LLNQLSNMNQINLFKLSLEELEAYHEYLRAIKKYADSIT"
                     /name="epsilon-antitoxin"
     gene            4490..5680
                     /locus_tag="QLQ35_RS14275"
                     /old_locus_tag="QLQ35_14275"
     CDS             4490..5680
                     /locus_tag="QLQ35_RS14275"
                     /old_locus_tag="QLQ35_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876136.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_282861590.1"
                     /translation="MSIISENQKQVYTLNEIEKRKVVEHNSLITSIAKMQKTALKMFEL
                     AVSCIDTENLPKDNIIYLSKKELFSFFDVSSASKHTRFKEAIEYMQKQAFFQIKEEKNK
                     GYEMTSIVPIPTVKWNSYNDDVMIQFNQFIMPYLIDLKAEFTQYKISELKELNSKYSLI
                     LYRWLAMNYSQYEHYSVKGGRRAEQVEAYRNPSITVKELREITDTINEYTRFQSFENRV
                     LKNAIEEINAHTSFNVSYEKVKKGRSIDSIVFHIEKKRRADDNSYKLEDTAFQVDKKQK
                     EQSEAVLVKQAMESKYTRLLLDNMLLSPYEMTDTSLMAGLQAHVYPLYDELKALRGLNG
                     VKDHLSYVRAKQEAYSKRNIAKYLKKAIEQYLPTVKLQDLEQPERASVRGKGEIHE"
                     /name="RepB family plasmid replication initiator protein"
     gene            5673..6263
                     /locus_tag="QLQ35_RS14280"
                     /old_locus_tag="QLQ35_14280"
     CDS             5673..6263
                     /locus_tag="QLQ35_RS14280"
                     /old_locus_tag="QLQ35_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB-like protein"
                     /protein_id="WP_073340479.1"
                     /translation="MSENLKTIKEIADEIGVSKQAVWQKIKKESSIDLRQFTSKKGNTV
                     YVDVDGQKVIKNAFFNKTSTKKRQQKVFVDDNVNNSVDGNPEGNEEILFLRNLVSELQS
                     EKKELHKLLDQQQRLALQDKKLLEEYKSEINELKALKMPQEDMKDGSSIRGEAQEEIER
                     LKAQLKSSEEERNKAKEKEPVKTESKKWWQLWK"
                     /name="RepB-like protein"
     gene            6275..9352
                     /locus_tag="QLQ35_RS14285"
                     /old_locus_tag="QLQ35_14285"
     CDS             6275..9352
                     /locus_tag="QLQ35_RS14285"
                     /old_locus_tag="QLQ35_14285"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003138440.1"
                     /GO_component="GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA]"
                     /GO_function="GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="WP_282861593.1"
                     /translation="MSHSEQMIENQFIQILSEKENQWTYRPDLKSEEALWQNFRGHLNR
                     INLAVLEEQLLTDKEFKQVKVEFSRLTATPFLASQWLRGENGVAQVLLEREDGKKVTLE
                     AFRNKDISGGTSSYEVVHQVVPDSSRVDRGDVSLLINGLPIIHIELKKESAKDGFMQAY
                     YQIQRYAEDGFFKGIYATTQIMVISNKVDTRYFARPSEDTAEAYARMKKFLFNWRTEDN
                     QTVSDLFDFTRTVLRIPDAHELISQYTILVDDQKNQKFLMALRPYQIHAIRKIRQKAAQ
                     HEGGFIWHATGSGKTITSFVATKLLAQNAIGVDRTVMVVDRTDLDAQTQDEFTKFASEY
                     HTGQTTGNSVANTLIVGIKNQKQLARNLLSSKNNNTILVTTIQKLSAAMRSAQQESEEK
                     GSNQFEKLRQEHIVFIVDEAHRAVSDEEMKRIKKILPNSTWFGLTGTPIFEENKKQENG
                     TFARTTSQQYGPLLHSYTTKNAMDDGAVLGFQVEYHSLISEEDQEVIVTQLNKGKLPDD
                     ALQQEKLLPTELYETDEHIRTMLQKIFNRRSVVKKFKVKNGFPTMSAILTTHSIAQAKH
                     IYRILKEMKDNETLLNGRQFDERHQLIDKDFPRVAITFSTNPDQLEKNEQDDELVEIMK
                     EYAKQFDASPYQDEKLYNQNINKRLARKEKQYQSDGQWLDFVIVVDRLLTGFDSPTIQT
                     LYIDREMNYQKLLQAFSRTNRIYTGKDSGLIVSFRKPFTMKENVQNTFRLFSNEKQNFD
                     QLIPKEYEEVKKEFIECSILYKQSEADLSDNPNDLKTMIAQVSAYQKLEKSYKALRSYD
                     QYEEDFEAFSEVVEQLPQYQGKTENIKTKIKEMIEDEGHPEEDFEKLLQEIAFSSQLNA
                     THKDVVDSFYINQLLKAIQLNEAGAVEKFEKEIQQKDPRIQQMYHTLKDQLINTAEEID
                     VAQLKETSIQNEIQRQLQKEAEEFGLSFDFLQSAMNEYQGDKKTIPYLTHLLDSMTLSK
                     EEFEAKTGEKYRRRTKVLEERLQQNFEHLQKWKEEL"
                     /name="HsdR family type I site-specific deoxyribonuclease"
     gene            9352..10947
                     /locus_tag="QLQ35_RS14290"
                     /old_locus_tag="QLQ35_14290"
     CDS             9352..10947
                     /locus_tag="QLQ35_RS14290"
                     /old_locus_tag="QLQ35_14290"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342416.1"
                     /GO_function="GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA]; GO:0009007 -
                     site-specific DNA-methyltransferase (adenine-specific)
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M"
                     /protein_id="WP_282861575.1"
                     /translation="MATGLNQQLWASADILRGKMDASEYKNYLLGLIFYKYLSDAQLRE
                     VYEQENGKTDTFPERSTQYAGFMEWYEEDKDDLIENIQPKQGYFIQPDQLFYHYRIKAD
                     NYEFNLTDLQAGFNELERQGEEFSGLFADIDLNSTKLGSNAQQRNVTITEVLRALDEID
                     LFEHNGDVIGDAYEYLIGMFAAGAGKKAGEFYTPQAVSRIMSEITSIGQESRAPFHIYD
                     PAMGSGSLMLNIRRYLINPNQVHYHGQELNTTTFNLARMNLILHGVDKERMNLNNGDTL
                     DADWPSEEPYQFDSVVMNPPYSAKWSAADKFLSDPRFERFGKLAPKSKADFAFLLHGFY
                     HLKESGTMGIVLPHGVLFRGAAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPTTVIIL
                     KKNRSRRDVLFIDASQDFEKQKNQNVLLDEHIDKIVSTYKKREDIERYAHVASFDEIQE
                     NDFNLNIPRYVDTFEEEEPIDLVAVNTNLLKINEELVQQDQTLLSLINDFSESEENQAL
                     IESMRLLLRGGHDE"
                     /name="type I restriction-modification system subunit M"
     gene            10940..12166
                     /locus_tag="QLQ35_RS14295"
                     /old_locus_tag="QLQ35_14295"
     CDS             10940..12166
                     /locus_tag="QLQ35_RS14295"
                     /old_locus_tag="QLQ35_14295"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="WP_282861576.1"
                     /translation="MSKKSPQLRFKGFTDDWEERKLGEVGYFYYGKSAPKWSVTEDAET
                     PCVRYGELYTKYGAKISNIYSHTNINPKNLKFSTGNEVLVPRVGENPLDFSLCSWLSIP
                     NVAIGEMISVYNTNENPLFISYYFRSQMKYEFAKRVEGGNVSNLYYSYLEDIDLYIPKL
                     EEQIAISKFFEKLDDTIALHQRKLDLLKEQKKGYLQKMFPKNGAKVPELRFAGFADDWE
                     ERKLEELALVLTGFPFKNEDFVDNGKYLVITNGNIQNESSTVDVSLGNRVDDLSDEIKE
                     RYYLNKNDILVTMDGTVGRTAKVVDDNMILAQRVARLVANKNTEFVYQWLNQGSFFNEM
                     TKVSHGGTIKHISLNEISNYSATVPSNNIEQQKIGSFFKKIDDTIALHQRKLDLLKEQK
                     KGFLQKMFV"
                     /name="restriction endonuclease subunit S"
     gene            12505..>12594
                     /locus_tag="QLQ35_RS14300"
                     /old_locus_tag="QLQ35_14300"
                     /pseudo=""
     CDS             12505..>12594
                     /locus_tag="QLQ35_RS14300"
                     /old_locus_tag="QLQ35_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /name="DNA recombinase"
     gene            13128..13319
                     /locus_tag="QLQ35_RS14305"
                     /old_locus_tag="QLQ35_14305"
     CDS             13128..13319
                     /locus_tag="QLQ35_RS14305"
                     /old_locus_tag="QLQ35_14305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064686950.1"
                     /translation="MLKKMYQKSASRQQILETPENDPKRSFRPKQNRNRENSTRNEPEQ
                     PPIKRDQSDHNRDHGPSL"
                     /name="hypothetical protein"
     Typing          4521..5596
                     /name="rep_cluster_570"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.35"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcgctaat ctcgtcttca ttaattccga tatagcgttt agtaattttc tcactgctat
       61 gaccaaatat ttccattaac gtggccacat ctttggtctt tttgtaataa tgatacccaa
      121 acgttttccg cagcgtgtgc gtcccaatat cgtctcttcc taacagctta gcgacctttt
      181 gaaacatctg atagactgta ttgacttcta catggccccc tttggtactg ggaaacaaat
      241 aatcctctgg ctccaagtct ttcgtgtagt cctggatcaa ctcctgcagg ctacttaaat
      301 ataaaatgcg tgttttcccg gttttttgct ccacaatacg tgggtttttc gaggaaatca
      361 cgtctttttt ctttaatttc acgatatcag acatgcgcaa accgctgtta atgccgatca
      421 aaaacaaaaa aacgtcgcgt tcggcatttt tgttgcgtct caaacagaat aaaaagtcgt
      481 tgatttcttg ctgcgttcgt aatggttgta cattataact cattgaactg gtctctcctc
      541 tcacaaaaca gatacatgat ttcgttcatt ataccacgaa atcatgtatt ggataccggt
      601 ataaaaaaag aaccctacag atatagggtt ctggatacac gattttatac aatttcatgt
      661 atcgaattat attacttaga taatgcgcca atctgttagt acaaaaaaaa gacaacgagt
      721 tttaatcgct gtcttttttt tgtactaaca atcaaactat ttattaatta aataaactca
      781 atacagtttg aatcgtacca ttttcgatca atatataaat tcctaagcca ataaacacta
      841 taggaacaag gattcgttca tatttttcta aggtttcgga aacaaaagaa atcttggcta
      901 atttgtaact gatatagcaa agaaccgcaa cagaaatagc aaatacgatt agagcggtga
      961 caatctctga aaaagataac gaggcaaagt aaggaatata aatccctaag ttatcgccac
     1021 cagaagcaat tgttattaag gcaacggtcc aaaagaagcg gtttgttcct cttgattcaa
     1081 gcttttcaac gacttcctct tcttcttcct cctcttcgcc aaccaatgcc actctgattc
     1141 ctaagaaaat cggaattaga ccgagaagcc caatgatcca atcttgcgga atgaaattca
     1201 gcacatatgc tgcaaataaa cttacggcta ctaagatacc tgttcctaga tattgacccc
     1261 aaaaaatctg acggagccct ttttttgtat gactttgaga aaaaataatc agcaagataa
     1321 acaaataatc aatgctggta gaaatataaa cagcgatagc tgaaaaaata ctttgtaaca
     1381 aaataacttc ctccaaacta agatttcata gatagatttt ctgataactt taaaaaaata
     1441 tgaatagcgg aaaggatgtc ttcattcgct aaagagtaat aaactacttt tccatcttga
     1501 cgtgattttg ccattccatg tttttttaaa aaacgtaaat ggtgtgacgt tgtagcgaca
     1561 ctagcattaa gtatttctgc taaatcacag acacacattt ccttataggt ttctaaagca
     1621 taaaaaattt taattctaga agaatcgctg aaacatttcc ctaacacaag taagtttgaa
     1681 aaatcttttt gatccaattt attttttatt acatttactt tttctttatg tacgatagcc
     1741 gcttggcaaa ctttttccat tcatctcacc tcacattcaa acgattgttt gaatgaagta
     1801 tatctatttg atcactcgtt tgtcaatcta ccttaattcc aatacactat tataagcaat
     1861 ttcatgtatt gtaagtgtcg attaattagt ttaagaaaaa tcccctgcca aaaaggcaaa
     1921 ggggattttt gtgttttgca cggaaaaaat agtcggatat tccgacgttt ttcgtgcctg
     1981 ttcacgtcga accttttcag cctaaaggcg aaaaaagggg cagagtggac agacggtttt
     2041 tagcctcgca gagaccacac tttacggcgt aaagtatagt gggttatact ttacttggaa
     2101 gtcgtaccga atcaagacca acaataggag gtttttttat gtccatggta gtagcgagaa
     2161 tgcaaaaaat gaaagttcct aatttaatcg gactaggaaa tcataaccaa tgaaaaactg
     2221 acaaaaactg acaatcattc gaataaagag attgacgtgt cacgctctca tttgaattat
     2281 gatttagtcg atagaactta taattataaa accgatattg aaagatatat taacgaaaat
     2341 aaatccagtc ctcgagctat tcggaataaa gatgagttat aaagattttt ttgataaata
     2401 attttagaca agagcgtagc gaattttaac aaacacgctt gacggaaaaa gagtacagct
     2461 tcacgtaagt gatctgtact cttttttagc acaaacctat caatttttcg taagaaaaat
     2521 ttggtgggta taagtgcgcc ctttggtata taaaaatgat agaaaaggag tggtactatg
     2581 tttcaagcta tagtttttga aactgaaaga ctgaaattct gtgctgcaac accaaatgat
     2641 attaatataa ttatgggaat ggagacccat gttgataatg gttattaaga tcaaattatt
     2701 tattcgattt tagatagtga atggagaaag aaaaaataat ttatttttac gaaaaaaagg
     2761 cgtctataaa attttatagg cgcctttttt tcgtaatttt ttaattacta ctcaattgga
     2821 atagattttt ttatttcttt gttactatct tttggcaagg ttagtttaag aattccctct
     2881 gaataagaag cctttatttc gtcttcttta acattttcta ataaatattg acgtcggaca
     2941 cttgttaaac tacgttcgct acgaatcaac cttccttttt tatcttcggt tgctgtatca
     3001 atttggtgtt ggccactaat tgttaatact ccgttttcgt aagtaacttg aatgtcttct
     3061 ttaggaatac caggaagttc agcttctaca aaatattcat tatcagtttc atgaatatct
     3121 gttttgatta aatttggctt aaaatcattg aataaattac gactaaagtc ttcgaaaaaa
     3181 ttaaaaggtg tcatgttata tgtgtctgag ttacgttttt gaatctcgtt tgacataata
     3241 atcaatcctt tctatatttt cttttcaatt attattttag tcaaaaaagg gcaattttca
     3301 agtattaagc tattttaaaa ttgtgaaatt atttttcatg tgatgctatc agcatatttt
     3361 tttatcgctc gtaaatactc atgataggct tctagttctt caagagataa tttaaataaa
     3421 ttaatttgat tcatgtttga taattgattt agtaagtttg tttgtaggtt tttagaatca
     3481 cttttatcga attcattatt taaaacaaag ttcaacattt ttgagtgaat cgctgattga
     3541 aattcattga ttatctcaat ttcaaatgtt tttctgtaat ctaatttcat attgtttgct
     3601 cccttctggt ttcgtttcgc tttgctcaac taaacattta cttttaatta aacagacttc
     3661 gctagtttaa tcaaaaataa acgtgcgaat aaaaagcaaa tcattttgaa agctcataaa
     3721 atgcgttcta aggcgtttta tgagctttag gtgtaaaatt tatgctcttt acttttaaaa
     3781 agcctgtatc agcttgacat tagtgtttaa attgatttta acctaatttt agggtgctta
     3841 cttctttttg ggtgcgctag tttattcaaa aggaatcctg taaataaaaa agtaacatac
     3901 tccacaaagc tctattttta gttttaaggg ggtttttgtt ctaattctgc ttttgggttg
     3961 ttagggcttg ccctgaccgt ctgtaagacg cttgattgcg tgatatgagt atttagctag
     4021 tcaaacagtt aaaacagctt atatgagcaa ttagagggaa tccaataaat tcctaaaagt
     4081 ggttttgatc ttttctttta gcgagtgaac gaagtgaacg ctgcaagtaa aatgtgagcg
     4141 tggttttcgc tcactccttt tttgatgact ttgaccttta tttttacatt tttgaaaaaa
     4201 ataaaaaaaa ggcgaagcct atattacatt tatcttatat attttattct tttgtttctt
     4261 ttgcgtggaa aaaaagtcag tggtagcaag gaaggacaga tttttatagc aacaaaaaac
     4321 gatgtatata gcaacaaaaa acgatgtata tagcaacaaa aaacgatgta catagcaaca
     4381 aaaacttgat taagttgcta tatatttgta taatgaaagc atagagaaaa ttgacgtcta
     4441 aatcacttct ctatgcttaa tcacaaaaca ctcacaaagg agtatttcta tgtctattat
     4501 atcagaaaat caaaagcagg tgtatacctt gaatgaaatc gaaaaacgta aagtggtcga
     4561 gcataatagc ttgatcactt caatcgctaa aatgcaaaag accgctttaa aaatgtttga
     4621 attagccgtg tcttgcattg atacagaaaa tcttcccaaa gataatataa tctatctttc
     4681 aaaaaaagaa ttattttctt tttttgatgt atcttcagca agtaaacata ctcgttttaa
     4741 agaagcgatt gagtatatgc agaaacaagc tttttttcaa attaaagaag aaaaaaataa
     4801 agggtatgaa atgacaagta tcgtcccaat accaactgtt aaatggaaca gctacaatga
     4861 tgatgtaatg atacaattta atcaatttat tatgccttat ctgattgatt taaaggcaga
     4921 atttacacag tataaaatct ctgaattgaa agaacttaat agcaagtaca gtcttatctt
     4981 atatcgttgg ttagctatga attatagcca gtacgagcat tatagtgtta aaggtggacg
     5041 gagagccgag caagtggaag cctaccgcaa cccctcaatc acagtgaaag agttgcgaga
     5101 aatcactgat acgattaatg aatatacacg ttttcaaagt tttgaaaacc gtgtattaaa
     5161 aaatgccatt gaagaaatca acgcccatac atcctttaac gtgtcttatg agaaagtcaa
     5221 aaaaggacgt tcgattgatt ctattgtttt tcatattgag aagaaacgcc gagcggacga
     5281 taacagctac aagttggaag atacggcttt tcaagtagat aaaaagcaaa aagaacaaag
     5341 cgaagcggtt ctagttaagc aggcaatgga aagcaagtac acacggttat tgttggataa
     5401 tatgctatta agtccttatg aaatgaccga tacgtcactt atggcaggat tgcaggcgca
     5461 cgtgtaccct ctctatgacg agctgaaagc cttacgaggg ttgaatgggg tcaaagacca
     5521 cctctcttat gtgagagcta aacaagaagc ctattcaaaa cgcaatatag ctaagtatct
     5581 caaaaaagca attgaacaat acctgccaac tgtaaaatta caagaccttg aacaaccaga
     5641 acgtgcaagt gttcgaggta aaggagaaat ccatgagtga aaatttaaaa accataaaag
     5701 aaattgcgga tgaaataggt gtttcaaagc aagctgtatg gcaaaaaata aaaaaagaat
     5761 cgtcaataga tttacgtcaa tttacgtcaa aaaaaggaaa taccgtttac gttgacgttg
     5821 atggtcaaaa agttattaag aatgcttttt ttaacaaaac gtcaactaaa aaacgtcaac
     5881 aaaaagtttt tgttgacgac aacgtgaata attcagttga tggcaatcca gaggggaatg
     5941 aagagatttt atttttaagg aatctagttt ccgaactcca atcagaaaaa aaagagcttc
     6001 ataagctatt agaccagcaa caacgtctag ccttacagga taaaaaatta ctagaagagt
     6061 ataaatcaga aatcaatgaa ttgaaagctc ttaaaatgcc tcaggaggat atgaaagatg
     6121 gctcgtcgat aaggggtgag gcgcaggaag aaattgaacg actgaaagct caattgaagt
     6181 catcagaaga agaacgaaac aaggcaaaag aaaaagagcc ggtaaaaaca gaatctaaaa
     6241 aatggtggca actatggaaa tgagggggga tcaaatgagt cattcggaac aaatgattga
     6301 aaaccagttc atacaaatct taagtgagaa agaaaatcag tggacttatc gtccggactt
     6361 gaagtcggaa gaagcacttt ggcaaaactt tagaggtcat ttgaaccgaa ttaatttagc
     6421 agtgttggaa gagcaactat taacggacaa agaatttaag caagtcaaag tcgagttttc
     6481 acgcttgacc gcaacacctt ttttagcttc tcaatggctt agaggagaaa acggggtggc
     6541 tcaagtttta ttagagcgag aagatgggaa aaaagtgact ttagaagcct ttagaaataa
     6601 ggatatctca ggaggaactt cttcttatga ggtagttcac caagtggtcc cagattcctc
     6661 tagagtagat cgtggagatg tgagcttgct gattaatggg ctcccaatca ttcatattga
     6721 gctcaaaaaa gagtccgcta aagacggttt catgcaagct tattatcaaa ttcagcgtta
     6781 tgcagaagat ggatttttta aagggattta cgcaaccact caaatcatgg tgatttccaa
     6841 taaagtcgat acccgatact ttgcaagacc tagtgaagat accgctgaag cctatgctcg
     6901 gatgaagaag tttttattta attggcggac tgaagacaat caaacggttt ccgatttgtt
     6961 tgattttact cgtacagttt tgcggatacc cgatgcccat gaattgatta gccaatatac
     7021 cattctcgtc gatgatcaaa aaaatcaaaa attcctcatg gctttaagac cttaccaaat
     7081 tcatgctatt cgtaagattc gtcaaaaagc ggcacagcat gaaggaggat ttatttggca
     7141 tgcgacaggt tcaggaaaga ccattaccag ttttgtcgca acgaaattat tagcacaaaa
     7201 tgcgatcggt gtcgatcgta cggtcatggt tgttgataga acagacttag atgctcaaac
     7261 gcaggatgag tttacgaagt ttgcctcgga ataccatacc ggacaaacga ccggaaattc
     7321 ggtagccaat actttgattg ttgggatcaa aaatcaaaaa cagttggctc gaaacctcct
     7381 atcatcaaaa aataataata cgattttagt gaccacgatt caaaaactct ctgcggctat
     7441 gcgaagcgcc caacaagaga gtgaagaaaa aggctcgaat caatttgaga agctacggca
     7501 agaacatatt gtttttattg ttgatgaggc tcatcgtgcg gttagtgatg aggaaatgaa
     7561 gcgaattaag aaaatattac ccaattcaac ctggtttgga ttaacgggga cgcctatttt
     7621 tgaagaaaat aaaaagcaag aaaatggaac ctttgccaga acgacgagcc agcaatacgg
     7681 gccactcctt cactcctata caaccaaaaa tgccatggat gatggggctg tgttaggctt
     7741 tcaagtcgag tatcattcac tgatttcaga agaggatcaa gaggtgattg tcacccaact
     7801 caataaagga aagttgccag acgatgccct ccaacaagaa aaattattgc ctacggaact
     7861 ttatgaaaca gatgaacata ttcgtaccat gttacaaaaa atctttaatc ggagaagtgt
     7921 ggtcaaaaag ttcaaggtaa agaatggttt tcctacgatg tcagccattc ttaccactca
     7981 ctcgattgcc caagccaaac atatttaccg gattttaaag gaaatgaaag ataatgagac
     8041 actcttgaat gggcgacaat ttgatgaacg tcatcaactg attgataaag atttcccaag
     8101 agtagctatt accttctcaa ccaatccgga tcaattagaa aaaaatgaac aagacgatga
     8161 attagtagag atcatgaaag agtatgcgaa acagtttgat gcctctcctt atcaagatga
     8221 gaaactctac aatcaaaata ttaataaacg gttggcccgt aaggaaaagc aatatcaatc
     8281 ggatggtcag tggctagatt ttgttattgt tgtcgatcgt ttattgacag gctttgattc
     8341 tccaacgatt caaaccttat atattgatcg agaaatgaac tatcaaaagc tccttcaagc
     8401 gttctcaaga accaaccgta tttatacagg aaaagattct gggttgattg tctcttttag
     8461 aaagcccttc accatgaaag aaaatgtgca aaacacgttt cgtctattct caaatgaaaa
     8521 gcaaaacttt gatcaactga ttccaaaaga atatgaagaa gtcaaaaaag aatttatcga
     8581 atgttcaata ctttataaac aaagcgaagc tgacctatcg gataatccca atgatcttaa
     8641 aacgatgatt gcgcaagtaa gtgcttatca aaagctagaa aagagttata aagcgctccg
     8701 aagctatgat caatacgaag aggactttga agcattttca gaagtagtgg agcagttgcc
     8761 acaatatcaa ggaaaaacag aaaatattaa aacaaagatt aaagaaatga tcgaggacga
     8821 ggggcatcct gaggaagact tcgagaagct tttacaagag attgcttttt cttcgcagct
     8881 caatgcgaca cataaagacg tcgtggatag tttttatatt aatcaacttt tgaaagcgat
     8941 ccaattaaac gaagcagggg ccgttgaaaa atttgaaaaa gaaatccaac aaaaggatcc
     9001 tcgaatccaa cagatgtatc acaccttgaa agatcaactc atcaatactg ctgaagagat
     9061 tgatgtggct caattaaaag agacatcgat tcaaaatgaa attcaaagac aacttcaaaa
     9121 agaagccgaa gaatttggat tatccttcga ctttttacag tctgcaatga atgaatatca
     9181 aggcgataag aaaacaatcc cttatttaac ccatttactg gattcgatga ctcttagtaa
     9241 agaagagttc gaagccaaaa cgggtgaaaa atacagaaga agaacaaaag ttttagaaga
     9301 acgactacaa caaaattttg aacatcttca aaaatggaag gaagaattat aatggcgaca
     9361 ggtttaaatc aacaactatg ggcttctgcg gatatcttac gtgggaagat ggatgcaagt
     9421 gagtacaaaa actacttact gggattaatt ttctataagt acttgtctga tgcgcagctg
     9481 agagaagttt atgaacaaga aaatggaaaa acagatactt tcccagaacg ttccactcag
     9541 tatgcaggat tcatggagtg gtacgaagaa gacaaagacg atttaatcga aaatatccaa
     9601 cccaaacaag gttactttat ccaacccgat caattgtttt atcactaccg cattaaagct
     9661 gataactatg aatttaactt gaccgactta caagcaggtt ttaatgagtt ggaacgtcaa
     9721 ggagaagaat ttagtggatt gtttgcggat attgatttaa actccacaaa attaggctca
     9781 aatgcacaac aacggaacgt gactattact gaggtcttgc gtgccttaga tgagattgac
     9841 ttatttgaac acaatggcga tgtgattggg gacgcttatg agtatttaat cgggatgttt
     9901 gcggcaggtg caggtaaaaa agcaggagag ttttataccc ctcaagctgt ttcacgcatc
     9961 atgtcagaaa ttacgtcgat cggacaagaa tctcgagcgc cttttcatat ttacgatcct
    10021 gcgatgggat caggttcttt gatgcttaat atccgtcgct atctcatcaa tccaaatcaa
    10081 gtccattatc atggccaaga gctaaatacg acgaccttta acttagcacg tatgaactta
    10141 atccttcatg gggtggataa agaacgcatg aacctgaata acggggacac tttagacgcc
    10201 gattggccgt cagaagagcc ttatcagttt gattctgtgg tcatgaaccc tccttactct
    10261 gcgaaatggt cagcggcaga taagtttctc tctgaccctc gttttgagcg ttttggaaaa
    10321 ttagcgccta aatctaaagc ggactttgcc tttctccttc acggttttta ccatttgaaa
    10381 gaatcaggaa caatggggat tgtcctgcct catggcgttc tctttagagg agccgcagaa
    10441 ggaaccattc gtcaggccct tttagaaatg ggagctattg atgcagtcat tggcttgccg
    10501 gccaatatct tttttggaac gagtattccg acaacggtta ttattttgaa gaaaaaccgt
    10561 tctcggcgtg atgtcttgtt tatcgatgct tctcaagatt ttgaaaaaca aaaaaatcaa
    10621 aatgtcctgt tggatgaaca tattgataaa attgtttcta cctacaaaaa gcgagaagat
    10681 attgaaagat atgctcatgt tgcaagtttt gatgagatcc aagaaaatga ctttaactta
    10741 aatatccctc gttatgtaga tacctttgag gaagaagaac cgattgattt ggttgcagtg
    10801 aataccaatc tccttaagat caatgaagaa ttagttcaac aagatcaaac actcttatct
    10861 ttgattaacg atttttcaga aagtgaagag aatcaggcct tgattgaatc gatgcgtctt
    10921 cttttgagag gcggtcacga tgagtaaaaa aagtccacaa ttaaggttta aaggattcac
    10981 ggatgattgg gaagagcgta agttagggga agtaggctat ttttattatg ggaaaagcgc
    11041 accgaaatgg tcagtgactg aggatgcgga aacaccttgt gttagatatg gtgaactata
    11101 tacaaaatac ggagcaaaaa tatctaatat ttattctcat actaatataa accctaaaaa
    11161 tctaaaattt agtacaggta atgaagtttt agtgcctcgg gtaggcgaaa atccacttga
    11221 tttctcattg tgttcatggc ttagtattcc aaatgttgca attggtgaga tgatttcagt
    11281 ttataataca aatgaaaatc cactatttat atcttattat tttagaagcc aaatgaaata
    11341 tgagtttgcg aagagagtag aaggtggaaa tgtatcaaac ctatattatt cttatctcga
    11401 agatatagat ttatacattc cgaagctgga agaacaaatt gctatttcta aattttttga
    11461 aaagttagat gacactatcg ctcttcatca gcgtaagtta gatttgttga aagagcagaa
    11521 aaaaggctac ttacaaaaaa tgttccctaa aaatggtgcc aaagttcctg aattgcgatt
    11581 tgcggggttt gctgacgatt gggaagagcg taagttagaa gaattagccc tagttttaac
    11641 aggctttcca tttaaaaacg aagatttcgt tgacaatgga aaatatttag ttattaccaa
    11701 cggaaatatt caaaatgaat cttctaccgt agatgtatca ttaggtaata gagtagatga
    11761 tctatctgat gagattaaag aacgatatta tttaaacaaa aatgacattt tagtcacaat
    11821 ggatggaacc gttggaagga ctgctaaagt ggtagatgat aatatgattt tagctcaacg
    11881 agttgcccga ctagtagcca ataaaaatac tgaatttgta tatcagtggt taaatcaggg
    11941 ttcttttttt aatgaaatga caaaagtgtc ccatggtggg actattaaac atatctcttt
    12001 aaatgaaata tccaattata gcgctacggt tcctagtaat aatattgagc aacaaaaaat
    12061 cggttcattc ttcaaaaaga tagatgacac tatcgctctt catcaacgta agttagattt
    12121 gttgaaagaa cagaaaaaag gctttttaca aaagatgttt gtttagggtc tataattaga
    12181 taataacccc tcaaaaagac tattaaaaca acccccgtta tctattggac aggtaatagg
    12241 ggttgttttt atgttttttt aatataatag aaccaaagat ggagggatta tttgggatgg
    12301 taggcatgct tttactagca atcatgttca tttttatccc tgtttgtttt cttatcgtaa
    12361 actgggagca attaaaaagt tgttttcaaa aaaataacag atgaaaccct tgctacaagc
    12421 ggatgtgcag aaatgagaca ccgacaagaa aataaataaa taatttaatt atttacttat
    12481 ttttttgaaa ggaatatatg atatatggtt caacagattg ttttacctat caaagattca
    12541 aatatcttac aaatggtgca ggatacacta cttgatagtt ttcgtgctgg tcgtataccc
    12601 ccccattttt ccaaattcca aatttttttc aaaaattttc cagcgctacc gctcggcaaa
    12661 attgcaagca attttaaaac catgagggaa tttcattccc tcaaactccc ttgggcctcc
    12721 tccgaccgaa ataaggggcg ctacgcttat tatttcattc aattatcggc tttcataagc
    12781 taacagacaa catcttcact gcaaagccac gctacgctca agggcttttc cgctacgata
    12841 acgcctgttt tgacgattat gccgacaact aaacgaaata aacgctaaaa cgtctcagaa
    12901 acgattttga gacgttttaa taaaaaatcg ataaattata ttggcaaatt ataaaaatgc
    12961 cgtagaacga aaaatagagc ccttaaaaag caaataaact ccaatagcaa taaaaaacaa
    13021 aaccaaaacc caaaagatga aaaataaata caacagcaaa aataaatcat agttcgattt
    13081 ttcgaacaat gcgcacttac acaccactcc aaaattgggt ggtttttgtg cttaaaaaaa
    13141 tgtatcagaa gtcggctagc cgacaacaaa tccttgaaac acctgaaaac gatcctaaac
    13201 gatcttttag accaaaacaa aaccgaaatc gcgaaaattc aacacgaaat gaaccagaac
    13261 aaccaccaat taagcgagat caatcagacc ataaccgaga ccatggacca agtctttaag
    13321 cagttaaacg ctgaaatcaa gcgaaccaat caaaaattaa ccttagaagt ggtggaaatt
    13381 ttagaaataa tcaaagtatc taagatatct atgctatctt agatactttt ttcgtgaatt
    13441 tatctagata tcaaaaaaca atgaattctt gaaaatattg tcttaaagtt agaaacctaa
    13501 acggaagttc aacaatg
//
