LOCUS       NZ_CP124878             2524 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae strain K82 plasmid p5, complete sequence.
ACCESSION   NZ_CP124878
VERSION     NZ_CP124878.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34578016
            Assembly: GCF_030020895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Zhao,Y.
  TITLE     Convergence of Plasmid-mediated Colistin and Tigecycline Resistance
            in Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Zhao,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) Department of Clinical Laboratory, The First
            Affiliated Hospital of Wenzhou Medical University, The First
            Affiliated Hospital of Wenzhou Medical University, wenzhou, zhejiang
            325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030020895.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:48:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,676
            CDSs (total)                      :: 5,551
            Genes (coding)                    :: 5,402
            CDSs (with protein)               :: 5,402
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 66 of 149
            Pseudo Genes (incomplete)         :: 84 of 149
            Pseudo Genes (internal stop)      :: 24 of 149
            Pseudo Genes (multiple problems)  :: 22 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124878.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K82"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="China:ningbo"
                     /lat_lon="29.868388 N 121.549792 E"
                     /collection_date="2016"
                     /collected_by="Ningbo Medical Center Li Huili Hospital"
     gene            1..546
                     /locus_tag="QKW38_RS28335"
                     /old_locus_tag="QKW38_28335"
     CDS             1..546
                     /locus_tag="QKW38_RS28335"
                     /old_locus_tag="QKW38_28335"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="WP_039004747.1"
                     /translation="MRERYLNKISNFDTARIVLFIAIQIISCIFMFWGAIPALIVFFSY
                     LAMRKECNLKPIEKSKDYISVYMLLTAALVLFLFVYKAGMFRRGGDYFLLVFVFILLYV
                     YHLAFHKLWLRTIDENSSIIIENGIFNFNRKNASVGFSRKDVNNIDTLLKLNEARKEGI
                     ISEDEFNKLKKRLNILND"
                     /name="SHOCT domain-containing protein"
     Typing          1320..1493
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.395"
                     /coverage="81.0"
                     /evalue="7.43e-58"
     Typing          complement(808..1354)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.405"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 atgcgcgaac ggtatttaaa taaaatatct aattttgata cggcaagaat agtgttgttt
       61 atagccattc aaattatctc ttgtattttt atgttctggg gggctattcc tgccttgatt
      121 gtttttttct catatttagc catgaggaag gaatgtaatt taaagccgat agagaagtct
      181 aaggattaca tatccgttta catgcttctt acagccgctt tggtactgtt tcttttcgtg
      241 tataaagctg gcatgtttag aaggggcggt gattatttcc ttctcgtttt tgtttttatt
      301 ttgctttatg tttaccatct cgcttttcat aaattatggt tgcgaacgat agatgaaaac
      361 tcatcaataa taattgaaaa cgggattttt aattttaatc gtaaaaatgc atctgtagga
      421 tttagtcgga aagatgtaaa taacattgat actcttctta aattaaacga ggctagaaaa
      481 gaaggtatta taagtgaaga tgagtttaat aaattaaaaa aaagactaaa tattcttaac
      541 gactgatttt ttgctaacta aaggtaaagc aagcttatga atctttgata agattgaata
      601 tcttcttgtc attgattttt tatcgtcgta atcttcttcc ctgcaaacga aaaaaccacc
      661 cggcaaggtg gtttttcgaa ggttcgcaag agttgacgct cttttgaacc gcggtaactg
      721 gcttggagga gcacagtcac caaaacttgt tctttcagtt tagccttaac cggcgcatga
      781 cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat
      841 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa
      901 cggggggttc gtgcatacag tccagcttgg agcgaactgt ctacccggaa ctgagtgtca
      961 ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     1021 ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct
     1081 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg
     1141 agcctatgga aaaacggctt tgccgcggcc ttctcgtttt cccgttaagt ttcttcctgg
     1201 catcttccag gaaatttcct cctcgcccgt aagccggtac cgctcgccgc agccgaacga
     1261 ccgagcgcag cgagtcagtg agcgaggaag cggaatatat ccggtatcac atattctgct
     1321 gacgcaccgg tgccaccttt ttctcctgct acatgaagca ctttcataaa gtgttactcc
     1381 gccgtaacat attaagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc
     1441 gccccgaaac ccgctaacaa cctctctgct actggatatc accccatgcc atctgaatat
     1501 gatgaacaac ggggttcgac tgatacgcag cttcccggcg aatatgacct ttttgattta
     1561 atggcgaagg ttactgaaga gatcctgcat gcccctgtgg attctgggcc gcctgtcggc
     1621 cgggaagcgt tatagctttt tcgcttgccc gcgtacgtgg taatacgccg gtattacctt
     1681 ttatttcttt attttcatgt aattacagtg ggtttttaat acctgggtat tactcgtgaa
     1741 aacgaaacat tccttttttt tcttttaatt catgcagttg cgatgaaaat cgcggctgcg
     1801 ttaggtgtat gaccattttg aggttgaaaa ataatcaacc atggagggtg atttacgcag
     1861 gttaagggga gatcgtgaca aaaaaagcgt tcctcactgt atcgttcatg caggcatgaa
     1921 cgggttattc ttcctgacta cagaattaca tcgctccttc agttcataca tcacttttac
     1981 aacactttta caacactcct tcggcttgta catcactttt acaacacttt tgcatcactt
     2041 ttacatcatt tgtacatcac ttttacatca ttccttcagc tcatacgtca ctttcatcct
     2101 ctgtgccatt tgtacatcac ccccttcctg aacgtgctga cagcgggttt tccggctgcg
     2161 ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatggga
     2221 agaacgccgg aaacaggact tcccctggct gaatggtgtg aatttccgcg ctatgcactt
     2281 gcgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg
     2341 tggtttgttg cctgttttac cggtttcccg tcagaaacgc cctcagagcc tctcaggcgg
     2401 tgcacgcaag gggtgttatg gtaaatgccc catattcagt tactgctttt ctattttttg
     2461 aaataatatt aatatcaggc gattgatttt aaattaatta actatgattg aatggtggtg
     2521 attt
//
