LOCUS       NZ_CP124877             5784 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae strain K82 plasmid p4, complete sequence.
ACCESSION   NZ_CP124877
VERSION     NZ_CP124877.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34578016
            Assembly: GCF_030020895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Zhao,Y.
  TITLE     Convergence of Plasmid-mediated Colistin and Tigecycline Resistance
            in Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Zhao,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) Department of Clinical Laboratory, The First
            Affiliated Hospital of Wenzhou Medical University, The First
            Affiliated Hospital of Wenzhou Medical University, wenzhou, zhejiang
            325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030020895.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:48:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,676
            CDSs (total)                      :: 5,551
            Genes (coding)                    :: 5,402
            CDSs (with protein)               :: 5,402
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 66 of 149
            Pseudo Genes (incomplete)         :: 84 of 149
            Pseudo Genes (internal stop)      :: 24 of 149
            Pseudo Genes (multiple problems)  :: 22 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124877.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K82"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="China:ningbo"
                     /lat_lon="29.868388 N 121.549792 E"
                     /collection_date="2016"
                     /collected_by="Ningbo Medical Center Li Huili Hospital"
     gene            join(5472..5784,1..11)
                     /locus_tag="QKW38_RS28295"
                     /old_locus_tag="QKW38_28295"
     CDS             join(5472..5784,1..11)
                     /locus_tag="QKW38_RS28295"
                     /old_locus_tag="QKW38_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001430281.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            220..>651
                     /locus_tag="QKW38_RS28300"
                     /old_locus_tag="QKW38_28300"
                     /pseudo=""
     CDS             220..>651
                     /locus_tag="QKW38_RS28300"
                     /old_locus_tag="QKW38_28300"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1207
                     /locus_tag="QKW38_RS28305"
                     /old_locus_tag="QKW38_28305"
     CDS             689..1207
                     /locus_tag="QKW38_RS28305"
                     /old_locus_tag="QKW38_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_072665564.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTTSG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            1214..1447
                     /locus_tag="QKW38_RS28310"
                     /old_locus_tag="QKW38_28310"
     CDS             1214..1447
                     /locus_tag="QKW38_RS28310"
                     /old_locus_tag="QKW38_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1841..2974)
                     /locus_tag="QKW38_RS28315"
                     /old_locus_tag="QKW38_28315"
     CDS             complement(1841..2974)
                     /locus_tag="QKW38_RS28315"
                     /old_locus_tag="QKW38_28315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123863911.1"
                     /translation="MKINSIQWPVTFFATSFGAGIFFLPQTVGPKVIGTHGTITILILS
                     SLASLLGQFTFYRFILITDKKEYVSSAILFIGKKAANAISIAFLLSMLLIAIINITTMI
                     NIICFSLSLNGSERIFVSLIISATLGSSFIFFNSKIEHLASGIAFPSLLIVLFLSIYFY
                     SHPAHPHSTEIQNISTSNLLTLFPVVLFSFNFSPCIQRFAQNKKDTAGDLRFFMFGILL
                     ILIFISFIVFSLSNIFSPSDIKNMREINSDALSYSANVTKNSFVAYISILTMILITSGA
                     YTGTLTGVVDTIVSLGFKNKTLIVFVVSVITFIAGEANFEIIKVIAALSTPIIAITVFI
                     IPSAFFLKTNCNSIFRKIMMTLNLLIGIGICFLAIIL"
                     /name="hypothetical protein"
     gene            complement(2986..3876)
                     /locus_tag="QKW38_RS28320"
                     /old_locus_tag="QKW38_28320"
     CDS             complement(2986..3876)
                     /locus_tag="QKW38_RS28320"
                     /old_locus_tag="QKW38_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015376142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021512263.1"
                     /translation="MPDLEIMPLQSPDFYKKNKRAIYEGYKCNCTKDWKKEDRFVVYKA
                     DCTGIDEIINTEISDDNIDTVIKLAEKYTSDKIIISGGHTVVNLNDRFSVSNEVEKSAK
                     FCIDYIIKSTHELNIKPDFLMEINDFYMEKSNGEDIDGGNIYRKLATSPYIIPEVINNY
                     IIEKQNQHNIKINCFYVSEKNMADRFKRHIKRKEKEKPFFKENNSVFMNVDGSSFEVIK
                     NNKPTCAAGNAATFRSIRYKISSNKTFDNYTSHIGVFPLCSMANVINGYKAAASFYSNF
                     NLPCLLIFFGTSCFK"
                     /name="hypothetical protein"
     gene            4053..4141
                     /locus_tag="QKW38_RS28325"
                     /old_locus_tag="QKW38_28325"
     tRNA            4053..4141
                     /locus_tag="QKW38_RS28325"
                     /old_locus_tag="QKW38_28325"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4087..4089,aa:Lys,seq:ctt)
                     /name="tRNA-Lys"
     gene            complement(4378..4536)
                     /locus_tag="QKW38_RS28395"
     CDS             complement(4378..4536)
                     /locus_tag="QKW38_RS28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072672540.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAAIGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(5217..5408)
                     /locus_tag="QKW38_RS28330"
                     /old_locus_tag="QKW38_28330"
     CDS             complement(5217..5408)
                     /locus_tag="QKW38_RS28330"
                     /old_locus_tag="QKW38_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_100783622.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          5047..5132
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          complement(4465..4996)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.59"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          2..1551
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.374"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5443..5783
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.246"
                     /coverage="97.0"
                     /evalue="1.88e-66"
ORIGIN
        1 atgatagtta aatttcatgc caggggaaaa ggtggtggca gtggtccggt tgattacctg
       61 ctggggaggg agcgtaatcg cgaaggcgca acggtgcttc agggtaatcc ggaagaagtc
      121 cgggaactca tcgatgccac gccatttgcg aagaaataca cgtccggtgt tctgtcgttc
      181 gcggagaagg aactgccgcc gggaggacgt gaaaaagtga tggcgagctt tgagcgtgta
      241 ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt gggtggagca ccaggacaag
      301 ggacggcttg agctgaattt tgtcattccg aacatggagc tacagaccgg aaaacgcctc
      361 cagccgtact acgaccgcgc agacaggccg agaattgatg cctggcagac gctggtgaat
      421 caccattacg gactgcatga ccccaacgcc ccggagaacc gccggacgct gacccttcct
      481 gataacctgc cggaaacgaa acaggcactt gctgagagcg ttacacgcgg tatagacgca
      541 ctttaccatg ccggagagat aaaaggccgt caggatgtga ttcaggcgct cacagaggcg
      601 gggcttgaag tggtcagggt gacgcgaagc agtatcagca ttgcagaccc gaacggcggg
      661 aggaatatca ggctgaaagg agcattttat gagcaatctt ttacagacgg gcgcggagtt
      721 cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaacg acgagttcag
      781 gaagctcggc gagtctgtca gcgaggctgt gacctcaaac gagacgaaaa tcagagacgc
      841 tatagccctg ttcacagcct cgacagagaa atcgctggaa aaacaccggg aaggggtgaa
      901 agaggcgatg atgcagcaca ggaaggacgt gttaaagctg gcagggaata cgggcatgat
      961 gttactgggg atagtctttc tcctgtttac cacgagtggc gggacgctct ggtatcttgg
     1021 cggacggata cagacgaacc tggaagaaat caggaagcag gaagagacat tgcagaaact
     1081 gaacgcgaag acatggggcg tggagtttgt gcaggacggg aacaggaaat tccttgtcct
     1141 tccgtacggg aaatcagcgg aggtgattcc ctttcagggg aaagagtggg tacatctgaa
     1201 ggagtgacac agagtgacag agctggaaat acatttgctg aacgccttag agcagctgca
     1261 acaggactat atgcagcggc tgagcgaatg ggagagcgcc ttcgtggaat tgcagaagat
     1321 gttttcgctt acgcaacggg acaacgcgat gctgaacgag cgggtcatgc agttgagtca
     1381 gcaggtgcag cacttgagcg agcagacaga acgcttgagc cagttataca gcgagaactg
     1441 gagataagag aggaacggtt gatacaggag cgcgaacatg tgttatccct ggaacgggag
     1501 cgtcagccgg aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat
     1561 gaaaaatggc agcttcagtg gattaagtgg ggggaatgtg gcctgtacca tctggttgca
     1621 taggtattca tacggttaaa atttatcagg cgcgatcgcg gcattttttc gggtggtttg
     1681 ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca
     1741 atattgttgt tatgttaaat ggtttggatg atccccccat tttccacatt tggttgtgac
     1801 gattaagata aactcgtcag aatatttggg tgaggaataa tcataatatg atggctagga
     1861 agcatattcc aatacctatg agcaggttta acgtcatcat tatttttcta aaaatactgt
     1921 tacaatttgt ttttaaaaag aatgctgatg ggataatgaa tactgttatt gctataattg
     1981 gggttgataa tgcagcaata acttttataa tttcgaaatt ggcttcgcct gctataaatg
     2041 tgataacaga tacgacaaaa acgattaatg ttttgttttt aaatccaagg gatactattg
     2101 tatcaacaac gcctgttagt gtacctgtat atgcaccgct ggtaattaaa atcatggtta
     2161 atatggaaat gtaagcaacg aaactatttt ttgttacgtt ggcgctatat gataatgcat
     2221 cgctgttaat ctcgcgcatg ttttttatgt ctgaaggtga gaatatattg cttaatgaga
     2281 acacgatgaa tgagataaat attaaaatta atagtatccc aaacatgaaa aaacgtaaat
     2341 caccggctgt atcttttttg ttttgagcaa agcgttgtat acatggtgaa aaattaaaac
     2401 taaataaaac aactggaaat agagtaagta aattacttgt actaatattt tgtatttctg
     2461 tagaatgtgg atgtgctgga tgtgaataga aatatataga taagaacagt acaataagca
     2521 aagatggaaa tgctattcct gatgcgagat gctctatctt tgaattaaaa aagataaaag
     2581 aagaaccaag ggttgcagaa ataatcagtg acacaaatat tcgctctgaa ccatttagac
     2641 ttaggctaaa gcaaataata tttatcattg ttgttatgtt tataattgct attaacagca
     2701 ttgataatag gaatgcaata ctgatagcat ttgcggcttt ttttccgata aataatatgg
     2761 cagaactaac gtattctttt ttgtctgtaa ttaaaatgaa tctataaaat gtaaattgcc
     2821 ctaataaaga tgccaaagat gataaaatca gtattgttat tgttccgtga gttcctatga
     2881 ctttggggcc tacagtctgg ggtaggaaaa aaatccctgc cccaaatgat gttgcaaaga
     2941 atgttactgg ccattggatg ctatttatct tcatttttga acctttcatt tgaagcatga
     3001 tgttccaaag aaaataagta aacatggaag attaaaatta ctataaaaag atgctgctgc
     3061 tttatagcca ttaattacat tagccattga gcacaatggg aatacaccta tgtgtgatgt
     3121 gtaattgtca aatgtcttgt tcgatgaaat tttatatcga atcgagcgaa atgtagctgc
     3181 gttacctgct gcacaagtcg gtttgttatt ttttatcact tcaaatgatg aaccatcgac
     3241 attcataaaa acagagttgt tttctttaaa aaatggcttt tctttttctt ttcttttaat
     3301 gtgtcgttta aaacgatctg ccatgttttt ttcactaaca tagaaacaat ttatttttat
     3361 attatgctga ttctgtttct ctataatata gttatttatt acttctggta ttatataggg
     3421 tgatgtggct agttttctat aaatattacc accatcgata tcttcgccat ttgatttttc
     3481 catgtagaaa tcatttattt ccatgaggaa atcaggtttt atatttagct catgtgttga
     3541 ttttattatg tagtcaatgc aaaatttagc cgatttttca acttcattac taactgaaaa
     3601 acggtcattc aaattcacaa cagtatgtcc accagatata atgatcttgt ctgatgtgta
     3661 tttttcggct agttttatta cagtatctat gttgtcatct gatatttctg tgtttattat
     3721 ctcatcgatt cctgtgcaat ctgctttgta tacaacaaat ctgtcttctt ttttccagtc
     3781 ttttgtacaa ttacatttat agccctcgta tattgctctt ttgttttttt tataaaaatc
     3841 tggactttgt aagggcatta tttctaggtc tggcatagtt atgcttcctt tgtcacaaag
     3901 aaaatatttt gctaattatg acttatacat atcaatgata gagatcatgt tagtgtttga
     3961 tcattgatgt ctcctttatt tatgtgatgc gtctctattt tttctcttat agtgtcttgg
     4021 atgttatttg ttaaatatgt attgtttttt gtggagaggt gggcgagtgg tctaagtcag
     4081 ggctctcttg aggccatatg cgacaagcgt atcgtaggtt cgaatcctac cctctccgcc
     4141 attttgttga tcgtttcccc caccatttgt tgaattgaga ggggttttga aaaaagatca
     4201 aaggatcctc ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa acaaaaaaac
     4261 caccgctacc aacggtggtt cgtttgccgg aatcaagagc taccaactct ttttcccgaa
     4321 ggtaactggc ttggtggagc gcagatacca aatactgtcc ttctagtgta gcctcagtta
     4381 ggccaccact tcaagaactc ttaatatctc aatccacctt gtccagttac caatggctgc
     4441 tgccagtggc gttaaggcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4501 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4561 ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acggcatcgg
     4801 cgctggcttg ttccggtgct ttgctttttg ctcacatgtt ctttccggcg ttatcccctg
     4861 attctgtgga taatcgtatt atcgcctttg agtgagctga caccgctcgc cgcagtcgaa
     4921 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaaa gcgcctggac gcgcattttc
     4981 tccttacgca tctgtgacgc atctgtgcgg aatttcacac ccggcatgag gcacttttca
     5041 tacaatctgc actgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     5101 tgggtcatgg ctgcgccccg acacccgcta aaacctgctg acgcgccctg gagggcttgt
     5161 ctgctcccgg catccactca caggcaagct gtgagcgtct ccggaagctg cttgtgtcag
     5221 agggtttcac cgtcatcccc gaagcgtgca aggcagctgc ggtaaagctc atcggcgtgg
     5281 tcgtgaagcg attcacagat atctgcctgt tcatccgcgt ccagttcgtt gagcttctcc
     5341 agcagcgtta atgtctggcc tctgatgaag cgcgccatgt taagggcggt tttttcctgt
     5401 ttagtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg ataccgatga
     5461 aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttacttgaac
     5521 gctgtgaggg taaacaactg gcggtatgga tgcggcgggt ctgcctgggg gagccggttg
     5581 cccgttccgg gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg
     5641 gaaataacct gaatcagaca gcccgtaagg tgaacagcgg gcagtggtct tccggtgacc
     5701 gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg
     5761 ccgtcaggga acagggggcg cggg
//
