LOCUS       NZ_CP124872             3631 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella quasipneumoniae strain M7 plasmid pM7f, complete
            sequence.
ACCESSION   NZ_CP124872
VERSION     NZ_CP124872.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34510250
            Assembly: GCF_030020875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Chen,Y. and Gan,Y.-H.
  TITLE     Whole genome sequence of multidrug-resistant Klebsiella pneumoniae
            M7 from Singapore
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Chen,Y. and Gan,Y.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) Biochemistry, National University of
            Singapore, 8 Medical Drive, Singapore 117596, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2023
            Assembly Method                   :: SPAdes v. v3.15.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030020875.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 05:04:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,325
            CDSs (total)                      :: 5,203
            Genes (coding)                    :: 5,097
            CDSs (with protein)               :: 5,097
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 42 of 106
            Pseudo Genes (incomplete)         :: 64 of 106
            Pseudo Genes (internal stop)      :: 23 of 106
            Pseudo Genes (multiple problems)  :: 20 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124872.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="M7"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="pM7f"
                     /geo_loc_name="Singapore"
                     /lat_lon="1.2941 N 103.7830 E"
                     /collected_by="National University of Singapore Hospital"
     gene            complement(join(3569..3631,1..867))
                     /locus_tag="QJS48_RS26575"
                     /old_locus_tag="QJS48_26575"
     CDS             complement(join(3569..3631,1..867))
                     /locus_tag="QJS48_RS26575"
                     /old_locus_tag="QJS48_26575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="WP_023307555.1"
                     /translation="MKEKIKKFGLPWICLFIVYFVCFLVVAATIIRHVINTTEEITLTT
                     YITPIFGLASLFVAMVTIRYNAVTNKEKNALDFVKEFKEKEIHDAMVEIYKTRRKYKNK
                     TNNRLNRKDISPYCEKAAAISQDDIKVIIPAEKKLELAEQIDLLTEEMKYTSSPSAKSK
                     IRGKISSLQKKLNDSNELEQICKFRSALIKVLNLMERCANGVRYGLYDEGLIYNSYGTQ
                     FIEIYELAFSFIKRRQDDEDKLFINAEWLAVKWTLQKEIQEDKPTFMTNQKRAIQHANE
                     GLKSHLKAPDKSKLEPLLNNLQKLKFPR"
                     /name="DUF4760 domain-containing protein"
     gene            complement(1343..1456)
                     /locus_tag="QJS48_RS26635"
     CDS             complement(1343..1456)
                     /locus_tag="QJS48_RS26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071787400.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPVRHAKAPLAAAIGN"
                     /name="replication initiation protein"
     gene            1861..2052
                     /locus_tag="QJS48_RS26580"
                     /old_locus_tag="QJS48_26580"
     CDS             1861..2052
                     /locus_tag="QJS48_RS26580"
                     /old_locus_tag="QJS48_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_011977837.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2052..2453
                     /locus_tag="QJS48_RS26585"
                     /old_locus_tag="QJS48_26585"
     CDS             2052..2453
                     /locus_tag="QJS48_RS26585"
                     /old_locus_tag="QJS48_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064154307.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKDEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHPAKLTESPTPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1344..1720)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="92.0"
                     /evalue="7.61e-175"
ORIGIN
        1 tgctttcagg tgtgatttca gcccttcgtt agcatgttgt atagctctct tttgattagt
       61 cataaatgtt ggcttgtctt cctgaatctc tttttggaga gtccatttta ctgctaacca
      121 ttcagcattt atgaataact tatcttcatc atcctgcctt ctttttatga atgagaaggc
      181 gagctcatat atttcaatga actgggttcc atatgagtta tatataagac cttcatcgta
      241 tagaccatat ctcacaccat ttgcacatcg ctccattaaa tttaacactt taattagagc
      301 tgatctgaat ttgcaaattt gttctaattc attgctatca ttgagttttt tttggagtga
      361 ggatattttt cctctgattt ttgattttgc gcttggggat gaagtgtatt tcatctcctc
      421 agtgagtaga tctatttgct cagcaagttc tagttttttt tctgcgggta tgattacttt
      481 tatatcatcc tgagatatgg cggcagcttt ttcgcagtaa ggtgagatgt cttttctgtt
      541 taatctattg tttgttttgt ttttatactt tcttcttgtt ttatatattt caaccattgc
      601 atcatgtatt tctttttctt tgaattcttt tacgaaatcc aatgcatttt tttctttatt
      661 ggtaacagca ttgtatcgta tggttaccat agcaacaaat aagctggcga gtccaaatat
      721 tggcgttata tatgtggtta atgttatctc ttcggttgta tttattacat gcctgataat
      781 agtggctgcg actactaaaa aacatacaaa gtagacaata aaaaggcata tccaagggag
      841 gccaaatttt tttatttttt ctttcatgtg tatgccgcta tgtgggttat agcggcgttt
      901 tactatcaga aaatgcctaa gtcaatctct ctaacgattt gggtcatttt ttcgacaacg
      961 ttaagcttct gcataggatt acctctcaat cgaggtggta atgattatca ttatcacctc
     1021 tacataatgt agagccccat atgtacatgg gtttcctcgg caagtaaatg cctacaagtt
     1081 taagtttagt taaaaaatgt gagttcgatt agtcattaag ggtaaattca ccatgaaaga
     1141 tcttcttgag atcctctgac gatcatcgta atcttttggc atacaaacga aaaaaccacc
     1201 cggcaaggtg gtttttcgag ggttcagtaa accggcaagt ttctgaaccg aggtaactgg
     1261 ctagtagagg agcacagtca ccaaaactgt tcgtttagtt tagccttaac cggcgtataa
     1321 cttcaagact atctcctcta aatcagttac caatggctgc tgccagtggc gcttttgcat
     1381 gtcttaccgg gttggactca agatgatagt taccggataa gacgcagcag tcggactgaa
     1441 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
     1501 ggcgtggaat gagacaaacg cggccataac agcggaatga taccggtaaa ccgaatggca
     1561 ggaacaggag agcgcacgag ggagccatca gggggaaacg cctggtatct ttatagtcct
     1621 gtcgggtttc gccaccactg atttgagcgt caaattctgt gatgcttgtc aggggggcgg
     1681 agcctatgga aaaacggccc ctgcgcggcc ttctccctct ccgtgctgcc tgtcctatgt
     1741 cgttatgtgc gcgttgctac cgctcgccgc agccgaacgg caggagcgta gcgactgagt
     1801 gagcgaggaa gcggaaaaga gactggtttt acactgagca ctgacgctct gaggcttctt
     1861 atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagca tcagtctcta
     1921 ctcctgctgg agaagcttaa cgagcttgat cttgatgctg aggccgacct ttgtgagaaa
     1981 ctccacgatg atgcggagca tcttttccgt acactgtcct cgagactgga tgcattacag
     2041 gatggtaact gatggccaga gtgtctatta gtgaggctgc ccgactgacg ggtaagagta
     2101 gaacaacact gcacaggtta attaaagcag gtgacctgtc cacctgtagc ggtgaacgca
     2161 atgcaaaaat ggtagatact tcagagcttt tacgtgtctt tggtcccttt gaacaaccga
     2221 aggatgaaca ccattctgga caggtctctg aacagcgtga tacagcctct gctgaccaga
     2281 gtgaacaggt gattcgtcat ctgaaacagg agattgaaca cctgaacact ttacttttgt
     2341 ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcacccgg
     2401 caaaattaac tgaatccccg acgccatggt ggaaattctg gaaaaaatcc tgaccccgtc
     2461 atgcttcagt tttcctctcc ggccatcccg gggagttcct gaaccttccc aaaaatcgtc
     2521 agatctctga acgctcctca ccactaaaaa tacccacacc ccacgcaaaa ttaaaatttt
     2581 gaagataact cattaatatc agttagttgt gggttattaa attacgcagt attactttct
     2641 gtaaggtgaa tccaagtcgt cggcgattcg tgtacgactt gccgacgact cgccatagag
     2701 tcatcgacga ctcactttag actatcagta acttatgacg acttgactct agagtcgaat
     2761 cgtcattttg gtgttttttt gaccttggcg actctagagt caagtcacga gtcgaatcgc
     2821 cggtgaatcg tgtggtgagt cgtccggcga cttgccataa cgtgattgcc ggccgtcaac
     2881 gaacccgatt cctgatcgtg ctgaaaaccg gttttccggc tgcgccggca acgcttcacg
     2941 gccacgcaat aaattgcgtc cccgttcacc gttttcggat aggaagaacg tcggaaacag
     3001 gacttaccct ggctgaatgg cgtgaatttt cgcgctatgc acttgcgtgc atactcatgc
     3061 atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt
     3121 ttaccggttt cccgtcagaa acgccctgag gccgttttag cggtgcgtac aatcagggct
     3181 ttatgttaaa tactctcgca ctagaaataa cagaggctca ctggagaacc tctgttgaaa
     3241 cttatctttt atcattctta gcaggtgcac tcatgcggtc tgtttccgca tttttcacag
     3301 tcattacaac gcttacagac ataatccacc tctttctgat tacactctct gcagtagctg
     3361 caagtgcaag cgctaccagg taagccgcgt ccgtcacaaa ttttgtccat tggatccata
     3421 tctacatcct ttgtgtggct tttttagcgt gcaatttcag tgatcacaac tgacgagttc
     3481 gtaaggacgg attgatgaga gggaaacccc tgtatttcag gggtttacat tccatataat
     3541 gcaaattacg cggcctgata aaaacttttt atcgcgggaa tttcagtttt tgtaagttgt
     3601 taagcaatgg ttctaatttg cttttatctg g
//
