LOCUS       NZ_CP124871             4063 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella quasipneumoniae strain M7 plasmid pM7e, complete
            sequence.
ACCESSION   NZ_CP124871
VERSION     NZ_CP124871.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34510250
            Assembly: GCF_030020875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Chen,Y. and Gan,Y.-H.
  TITLE     Whole genome sequence of multidrug-resistant Klebsiella pneumoniae
            M7 from Singapore
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Chen,Y. and Gan,Y.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2023) Biochemistry, National University of
            Singapore, 8 Medical Drive, Singapore 117596, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2023
            Assembly Method                   :: SPAdes v. v3.15.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030020875.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 05:04:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,325
            CDSs (total)                      :: 5,203
            Genes (coding)                    :: 5,097
            CDSs (with protein)               :: 5,097
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 42 of 106
            Pseudo Genes (incomplete)         :: 64 of 106
            Pseudo Genes (internal stop)      :: 23 of 106
            Pseudo Genes (multiple problems)  :: 20 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124871.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="M7"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="pM7e"
                     /geo_loc_name="Singapore"
                     /lat_lon="1.2941 N 103.7830 E"
                     /collected_by="National University of Singapore Hospital"
     gene            complement(join(3827..4063,1..465))
                     /locus_tag="QJS48_RS26560"
                     /old_locus_tag="QJS48_26560"
     CDS             complement(join(3827..4063,1..465))
                     /locus_tag="QJS48_RS26560"
                     /old_locus_tag="QJS48_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(999..1253)
                     /gene="mobC"
                     /locus_tag="QJS48_RS26565"
                     /old_locus_tag="QJS48_26565"
     CDS             complement(999..1253)
                     /gene="mobC"
                     /locus_tag="QJS48_RS26565"
                     /old_locus_tag="QJS48_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1432..3579
                     /gene="mobQ"
                     /locus_tag="QJS48_RS26570"
                     /old_locus_tag="QJS48_26570"
     CDS             1432..3579
                     /gene="mobQ"
                     /locus_tag="QJS48_RS26570"
                     /old_locus_tag="QJS48_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(1255..1431)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1432..1790
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3828..4063)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.0299999999999996e-120"
     Typing          1346..3576
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgctggagc agctcgtaaa tgcggatgct gtaggtgctg gaaaggctgg agacgttctt
       61 cacgactacc cgcgtaaact gccccttgag ctgtgtcaga tacggcatga tggcatcgga
      121 gaaggtgata tctactttcg cctcaccttt gaaatactgc gcccggtact gcacccagcg
      181 gaactcttct cgtttttcct cgttcttgat gattactgac ctgtcccaga ggcgatctat
      241 cgcttccttg agcctagctt cagcgtgctg ccgtcccatg tcaggaaagg catcatgaaa
      301 ctctgcagcc gtgatggtca tggttttttg ttcatccggc gaaggggaat cttctccgga
      361 ttttaggcgg ctgatacaga ccagcaggaa gcgctgttcc tgcaaagtga gcttgtagct
      421 tgcctcgata agggcgtttg atttgtaagc tatcagatct gacatttcgc ttgctcgttg
      481 ctattggtgg cgttttttta ctgtacgcca cttatcatat gggtggaagt tttttttttg
      541 caaccccctt tttctgtgga ttacaccccc aaaagctcca cctttcaccc ccaaaagctc
      601 cacctttcac ccccaaaagc tccacctttc acccccaaaa gctccactta tcttgccaga
      661 acatctttaa aatcatggtg ttacaagacc taaaaaggtt ttaaaaaaaa agatctaaaa
      721 ataagatctt aaaaacttaa cggcgagcct gtggataact tggggtgctg aaaacaaaaa
      781 accaataaaa acaatgcgtt gcaataaagc agaattgcaa gtttcgaata caccccctcc
      841 aaccctgtgg aaacccagta ttcatgcggg ttcagcgcat atcacctgcc gaaaaaccgt
      901 gcgtatgtgc tcaacctggc gactgattca cttccgcttc ctcgcccact cgctcgctaa
      961 cgctcggtcg ctggggctgc ggcgagcgga cgatacaatc actgttctgg cagcgcctcc
     1021 agctcgttaa tcagcggcag tatgcgcgtc acatcagcct gtcgtgtcac ctctgccttc
     1081 aggaactgga gtaaccaggc acgcaactgc acctcctccg gctttctgac ctttgccagc
     1141 aatgcagcac ccacaacaac cttctgcgct gtgtccaggc tccggctctt cgccttcagg
     1201 cgctgtaatc tcgcctcagc atcagcaatc ttctgttcga gcgttctgct catgatgtaa
     1261 ctccgtgcgc gatgaaaaat cgcattttag cgcgtcactg gtagtttaaa aactgagctg
     1321 gcataatgca cggcaaatca cgaagtgcgc acttatacaa tctccactcc gtttcgattg
     1381 tgtgcgccct gcggggctga aagaaaacta caaaaaggca ttacggcaga aatggcgatt
     1441 tatcatctca gcatgaaaat tatctccaga agcagcggct acagcgccgt agcttccgct
     1501 gcctatcgtt ccggttcact gatgcttgat gagcgtaccg gactgactca cgactacacc
     1561 cgcaaaagcg gtgtggctga ggctgtgatt ctgacccccg ccaccgcccc tgcctggtgt
     1621 acgaaccgtg ccgaactctg gaatgccgtt gagaaggccg aacgccggaa gaactcacaa
     1681 cttgcgcggg aaattgaact cgccatcccc cacgaactgc cgcaggatgc cgcacgggaa
     1741 accgttctcg cctttgtcag agaaaacttt gtcagtcagg gcatgattgc tgatgtggcg
     1801 ttccatcaca tggataaaac aaatccccat gcgcacatca tgctgaccac gagggctatc
     1861 ggacctgctg ggttcagcgg aaaggtcagg gactggaacg accggacaca tgccgagacg
     1921 tggcgggcat catgggctga ccatgcaaac agggcactgg cgaacgccgg ttatcaggaa
     1981 gagatagacc atcgttcata cgagcgacag gggcttgaga aaacgcctgg tattcacctc
     2041 ggaaagtctg cctgtgcgat ggaaacacgc ggtatcgaaa cagaacgcgg cgaacagaat
     2101 cgtctgatta acaggcttaa cctggaaata cagatttccc gcaccgaact caggaacgga
     2161 gggctgacag agcctgcccg ccgtgtggct gaccttctga atatcgggct tcctgacaac
     2221 gcaacttcgt acacactacg tgacattatc gaagcgttgc cgaaggacag taactcggca
     2281 tggaaactca ccagtaatgc catgtcgatg atggcagata tgcaggcgac gcgcagacgc
     2341 tgggaagagc tgaacaccga acgaaaggaa gcgatgagcc atgctgccag cctgaaaaaa
     2401 tcatcgccgt tttcgtccgg tttttcccgt attcccctga tgcaatgggc ggcaccggaa
     2461 taccgtagtg agcagggaaa aatccagtct ctgagccagc aaatggaaaa attacgacag
     2521 cattaccgga aggtggagaa acaggacatt cccgcgagtc agatggcatt tgaaaaacag
     2581 tggcagcagt ggggggaatc cggccttgca gctctcagag cccagttgct taaacgtgaa
     2641 gaggaattgc gccggaagga acaggaagat actgaacgta gtcgcgcgga gcagctcagg
     2701 aaaaatgaca atgccgttat ggattcaggg gtgctgagag cagtcgttac ggggtatggc
     2761 gaggctccca tgcctggtaa cggggaaatg acctgttacc ttctgcttca caaccggaac
     2821 ggagaatata cgctctgggg gaacgagctg gagaaatacc gcaccagaat ctttgagagt
     2881 gttgatttaa tgcgtgacag gagtggttat atctgtgacc gaagcgaaat cggccaacgt
     2941 ccgccactgc aacgtgttta ttcctctgca acctttgagc aactgctgac gcaggtttgc
     3001 cagacatggc cgcaacatac ccgcaatctc cgccagccga agacatggcc ggagtctttc
     3061 tgtcttggag aagaccgcca gccagccatg ccatcaccag cggcaaggaa ggtggatttt
     3121 atgcagggac gtctgccacc gacgctgatg ccggtcatga gttctgtcga ccgggaaacc
     3181 cgccagcttc agcttttgct ggtcatgggc gtggatgact ctctgggcgg tgttatccgg
     3241 cttaatggta cgctgtatcc cgcgtttgcc gttccctcag ccgataacag gcatctggtc
     3301 atcagtgcgc tgaccgataa ggggttacgg tatgcagggt atggtgttgc cgtaaatcac
     3361 gatgcagaca gccacatatc acctgcacca gaactgatgc agtttcatct gaaaacccgt
     3421 gaggcaccgc tttttgctgc cgtcaacaca ccagaaaaac agccggatca tctgttccgc
     3481 tcgctgggtt ttaatcgtac ctgggaggaa tggcagcgcg aggaagatgc acgaacacac
     3541 accaccgaga ggagacatga tcgcggttgg tcacagtgaa tgtgaacaat attttgtttt
     3601 gtttaacatc gtgttatgtt cttcgatgtt catcgacagg gatgaatgca agaagcacac
     3661 acacataatg caagcatagt tttgtttttc cgcccccaaa aattgggggt tcttttttcg
     3721 ctgcattaga gcgcttttga cgcaattttt cacttcaata gtaccttcgc actactaaca
     3781 caaaaaaaac gcagcaggga gcgttttagg tgccttaaaa tcgattttag atcgtcatct
     3841 tgatctgttt gtcttccttg aagcgaaaat gcagggctac aaccgtgcgc ccctttttga
     3901 tcgtctcaac agttacaacc agatcgcttt tttcgttcag ttcggcgatg gctggcttga
     3961 ttatcatctt attcaagtct ttgaattgtt gatatttatc ttcaacggca agcatggttc
     4021 ggaaatcatc gatagctatc gttctgtcac cggtgctgcg gaa
//
