LOCUS       NZ_CP124857             4340 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00376 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP124857
VERSION     NZ_CP124857.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16505100
            Assembly: GCF_025398395.2
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2020CK-00376
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 43.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025398395.2-RS_2024_06_02
            Annotation Date                   :: 06/02/2024 05:19:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,373
            Genes (coding)                    :: 4,286
            CDSs (with protein)               :: 4,286
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 35 of 87
            Pseudo Genes (incomplete)         :: 52 of 87
            Pseudo Genes (internal stop)      :: 16 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124857.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00376"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA"
                     /collection_date="2020-02-17"
     gene            239..1555
                     /locus_tag="N5929_RS22405"
                     /old_locus_tag="N5929_22485"
     CDS             239..1555
                     /locus_tag="N5929_RS22405"
                     /old_locus_tag="N5929_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_072203536.1"
                     /translation="MIVSLVVENFRSIKERTEFSLMASGSAGHLDEHVYRDESISFGVL
                     RSAGIYGANASGKSNLLMAFEAIKCIATETGRLKDEESIPCYEPYLLSEEMRKSPVSFE
                     VEFITKDKLRFNYKVKFNRSEVIEESLDYYPSKNKANLFKRDESDDWESIKFGSHYKGG
                     SKRIAFFKNNSYLAKAGDNASSPEIIKTAYNYIERSLRHIGLNEKIRLSSFGDRDVVVK
                     KTADMLCLFDTGISEITYKQNELDKIPFQMSDDAPQEIKDMITEDFGFSYLFSHKTESG
                     KGVKMPLRRESEGTQKLFEIIPLIYSAFESGMTVIVDELDSSLHSHIATIIIRLFNDPS
                     VNKSGAQLIFTTHNLNLMAPDKMRRDQVWFCEKNNGKTKVYSLDDFDKKKVKSSTPYAA
                     WYDDGRFGAVPDINYDKIRCLLSGEDLVVDDSVNFDDLF"
                     /name="ATP-binding protein"
     gene            1577..2215
                     /locus_tag="N5929_RS22410"
                     /old_locus_tag="N5929_22490"
     CDS             1577..2215
                     /locus_tag="N5929_RS22410"
                     /old_locus_tag="N5929_22490"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_058685403.1"
                     /translation="MAKRESKKITVPNIMYIFCEGEKTEPYYIESFINDRANGKSKVIR
                     IPKEKSTSPEHLLDAALAKKKSSSTADGDVFWVVYDKEDIKSRPVSMHEKVWNKAKSNG
                     VNVAISCVCIEFFILLHFSFTDASYSSFDNLKSRSPLNACFKRIGVDDYDKASDKTYHY
                     LKQRVDTAIANAERLEDKIVKDSEFCINEPYKIMAYTSFHKLLKNILEF"
                     /name="RloB family protein"
     gene            complement(2413..2526)
                     /locus_tag="N5929_RS22415"
                     /old_locus_tag="N5929_22495"
     CDS             complement(2413..2526)
                     /locus_tag="N5929_RS22415"
                     /old_locus_tag="N5929_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059446996.1"
                     /translation="MHEPPVQSDRCALSGNYHLESNPERHDKAPLAAATGN"
                     /name="hypothetical protein"
     gene            2947..3138
                     /locus_tag="N5929_RS22420"
                     /old_locus_tag="N5929_22500"
     CDS             2947..3138
                     /locus_tag="N5929_RS22420"
                     /old_locus_tag="N5929_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_058685416.1"
                     /translation="MTKQEKTALNMAKFIQGQSLLLLEKLNELDLDREADMCEKLHEDA
                     ELLCRTLSGRLHALQDAG"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3138..3524
                     /locus_tag="N5929_RS22425"
                     /old_locus_tag="N5929_22505"
     CDS             3138..3524
                     /locus_tag="N5929_RS22425"
                     /old_locus_tag="N5929_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_058685415.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKTGELSTCYGDRNARMVDISE
                     LIRVFGTLEQRPVEQVSGQVNEQTDTGVSAQSEQVIAQLRQEVEHLKTLVSAKDSHIDS
                     LKQAMLLIEHRHTGDSSWWKFWKR"
                     /name="helix-turn-helix domain-containing protein"
     Typing          4028..4106
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.185"
                     /coverage="89.0"
                     /evalue="2.56e-13"
     Typing          complement(2410..2785)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.668"
                     /coverage="88.0"
                     /evalue="3.81e-158"
ORIGIN
        1 cgggtggttt gttgcctgtt ttaccgttct gccgtcagaa acgcgctcag gccgcctgag
       61 cggtgcgcgt aatgaagcgt tatggtaaat cctcagtaga gggattcatt tacaaatagt
      121 catggcagat atcgcctaat cgctaaaaaa gttttgattt tttgatatca atagataatt
      181 atctattgat acgctttttt ttcgcttttt tggcgaaaaa tggctctaag gagtaattat
      241 gatagtttca ttggttgttg aaaatttccg ttctataaaa gaacgcacag agtttagctt
      301 gatggcttct ggttcggctg ggcacttaga tgaacatgtg tacagagatg aaagcataag
      361 ttttggtgtt ctaagaagtg ccgggattta tggagcaaat gcttccggaa agtcgaatct
      421 actcatggct tttgaggcta taaagtgcat cgctactgaa accgggaggc ttaaagacga
      481 agaatcaatc ccatgctatg aaccatattt actatctgaa gaaatgcgga aatcccctgt
      541 ttctttcgag gttgaattca taaccaaaga taagctcagg tttaattata aagttaaatt
      601 caatagatct gaggttattg aagaaagctt agattattac ccatccaaaa acaaagccaa
      661 tctttttaaa agagatgagt cggatgattg ggaaagtatt aagtttggga gtcactataa
      721 aggtggtagc aaaagaattg cattctttaa aaataactca tatttagcta aagctggaga
      781 taatgcatca tcaccagaaa taataaaaac agcctataac tatattgaaa ggtcgttacg
      841 gcatattgga ctaaatgaaa aaataagact ttcaagcttt ggtgatagag atgttgttgt
      901 taaaaaaaca gctgatatgc tttgcctttt tgataccgga attagtgaga taacatacaa
      961 gcaaaatgag ttagataaga taccatttca aatgagtgat gatgccccac aagaaattaa
     1021 ggatatgatc acagaggatt ttggatttag ttatttattc tctcataaaa ctgagagcgg
     1081 caagggtgta aaaatgccat tgcggcgtga gtctgagggt actcaaaagc tttttgaaat
     1141 aatacctctg atttacagtg catttgagag tgggatgaca gttattgttg acgagcttga
     1201 cagtagcctt cactcgcata tcgcaaccat aatcattcgc ttgttcaatg acccttcggt
     1261 taataaatct ggtgcgcagc taatattcac aacgcacaac cttaatctca tggctcctga
     1321 caaaatgagg cgggatcagg tttggttctg tgaaaaaaac aatggaaaga caaaggtata
     1381 cagccttgat gattttgata agaaaaaagt aaaatcatct actccatatg cagcttggta
     1441 tgatgatgga cgtttcgggg ctgttcctga tatcaattac gataaaatta gatgcttatt
     1501 gtctggtgaa gatcttgtcg tggatgatag cgtaaacttt gatgatttgt tttgattttg
     1561 tttcttgagg ttttaaatgg ctaaaaggga aagcaaaaaa atcaccgtac ctaatattat
     1621 gtatatattt tgtgagggtg agaagacaga accttattac attgagtcgt ttatcaatga
     1681 tcgagccaat ggcaaatcaa aagtaataag gattccgaag gaaaaaagca catcgcctga
     1741 gcatttgctt gatgctgcct tggcaaagaa aaaatcatca tcaacagctg atggtgacgt
     1801 attttgggtt gtttatgata aagaagatat caaaagcaga cctgtttcaa tgcatgagaa
     1861 agtttggaat aaagcaaaaa gcaatggtgt taatgtagct atttcttgtg tttgtataga
     1921 gttttttatt ttgcttcact ttagttttac tgatgcgtcg tactcgtcat ttgataactt
     1981 gaaatcaagg tcaccattaa atgcctgttt taaacgaatc ggtgtggatg actatgacaa
     2041 agctagcgat aaaacttatc attatctaaa gcagcgagtc gatacagcta ttgctaatgc
     2101 agagagacta gaagataaga ttgtcaaaga tagcgagttt tgtattaatg aaccgtataa
     2161 aattatggcg tatacaagct tccacaagct tttgaaaaac attttggaat tttagtttat
     2221 cttgaccttt aactctctga aaggtaatct tcttaaaaga aaaaaccacc gctaccaacg
     2281 gtggtttttc aaggttctcg gagctaccaa ctctttgaac cgaggtaact ggcttggagg
     2341 agcgcagtca ccaaaactgt tcgtttagtt tagccttaac tggcgtataa catcaagact
     2401 aactcctcta aatcagttac cagtggctgc tgccagtggc gctttgtcgt gtctttccgg
     2461 gttggactca agatgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc
     2521 gtgcatacag tccagcttgg agcgaactgc cttcccggaa ccgagtgtca ggcgtggaat
     2581 gagaaaccgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     2641 gcgcacgagg gagccgccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2701 ccaccactga tttgagcgtc agattctgtg atgcttgtca ggggggcgga gcctatggaa
     2761 aacagctttg ctgcggcctt atctttttcg tctttctttc ccggcaggta tccctttaat
     2821 ctttgggtaa cttcgcgagt ccgtcctgct cgccgcagac ccgtgaccga gcattgcgag
     2881 cgaacgggcg aggaagcaga atgtagtctg tctccacatt acgcacgtac gcactgaggc
     2941 ttgctcatga cgaaacaaga gaaaaccgcg ctgaatatgg caaaattcat ccagggacag
     3001 tccctcctgc ttcttgagaa actcaatgag ctcgatctcg acagggaggc ggacatgtgt
     3061 gaaaaacttc atgaggatgc agagctactc tgcaggactc tctccgggcg gttacatgca
     3121 ctacaggatg cgggttaatg gcaaaagtat cgatcagtga ggccgcacga ctgaccggaa
     3181 agagccggac aacactgcac aggttgatca agacaggtga actgtccact tgttacgggg
     3241 acagaaatgc ccgtatggtc gatatttctg agcttatccg tgttttcgga acccttgagc
     3301 aacgtccggt tgaacaggtt tctggacagg tcaatgaaca gactgacaca ggtgtttccg
     3361 cgcagagtga acaggtcatt gcacagctga ggcaggaagt ggaacacctc aaaacgctag
     3421 tttccgccaa ggattcacac atcgacagcc tcaaacaagc catgctgctg atcgagcaca
     3481 ggcataccgg ggattcatcc tggtggaagt tctggaaacg ctgagaacgc ctcagttgcc
     3541 ggcggggctt gtctgattta ctgaatgact taatccaaac ctgttttttc ggccggtccg
     3601 gacacagcgc tcgccgctct cttccttcag ggtcaaaaca tcacacccca cgcaagactg
     3661 cgtcttgcag atttatcctt ccttatcagt ggtataagac cagatgtttc aggcgattta
     3721 catgtatccc ggaaaatgtc acacaatcca cacacctgtt ggtgccggag ccggcactgt
     3781 catatcgccg gtttgtggct atctttattt atcagttagt tggatgtcac acaatccggc
     3841 actggtgccg gatccacata cctgtataac gccgcgcagt gccggatccg actacatgat
     3901 attcatccag gtggccgtat tgtgttactg atttatatcc agtgtggtgc tggatccggc
     3961 actgtggctt gccggcattt tcagggtggg ggcgcggctt caatttttct gagggtatca
     4021 gcgccacggg acaggatgtg catttgtaat accgcgcaca cgcggcacgc caaatgctcc
     4081 cgtctgggct cgccgccccg acacccggca agtgcttcac ggccacgcaa tgaattacgc
     4141 gcccgttcac cactaactgt atgccagccc gatttttcgc atgcgaaaaa ttatccgtca
     4201 gagatgcacg cctgcgcggt cgtaaagtgt gaaaaatgcg ggttatgatg tgtttttggt
     4261 tggatcaatc agagtcattc acctggctgc atagccattc actgcgccag aattcatgcc
     4321 ctgcgtttct ggcggatttt
//
