LOCUS       NZ_CP124817             9547 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Citrobacter freundii strain CF12 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP124817
VERSION     NZ_CP124817.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14640084
            Assembly: GCF_030007575.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Fernandez-Calvet,A., Toribio-Celestino,L., Alonso-del Valle,A.,
            Sastre-Dominguez,J., Valdes-Chiara,P., DelaFuente,J. and San
            Millan,A.
  TITLE     The distribution of fitness effects of plasmid pOXA-48 in clinical
            enterobacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9547)
  AUTHORS   Toribio-Celestino,L. and San Millan,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2023) Microbial Biotechnology, Centro Nacional de
            Biotecnologia-CSIC, Darwin, 3, Madrid 28049, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030007575.1-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 05:08:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,966
            Genes (coding)                    :: 4,877
            CDSs (with protein)               :: 4,877
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 22 of 89
            Pseudo Genes (incomplete)         :: 61 of 89
            Pseudo Genes (internal stop)      :: 19 of 89
            Pseudo Genes (multiple problems)  :: 11 of 89
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124817.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CF12"
                     /isolation_source="Human gut"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="unnamed3"
                     /geo_loc_name="Spain"
                     /lat_lon="40.416500 N 3.702560 W"
                     /collection_date="2014-10-31"
                     /collected_by="Ramon y Cajal Health Research Institute
                     (IRYCIS)"
     gene            complement(join(6882..9547,1..2179))
                     /locus_tag="QLG15_RS25360"
                     /old_locus_tag="QLG15_25370"
     CDS             complement(join(6882..9547,1..2179))
                     /locus_tag="QLG15_RS25360"
                     /old_locus_tag="QLG15_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="WP_282626386.1"
                     /translation="MNALSALLTKIEQASPTQRDKGTTFENLCVQYFLHEPKYAELYSD
                     VLSYGGWVSQYGETVGITKKKDDGIDLVAVTKTGEFHAIQCKNYNQTKIAKKDIDSFLA
                     ASDKTYFTLRYIVASTDNWTEEAKNMLRDKAVPVTALSLTDLEQSALDWSQFDFDPAYK
                     PVMKAKKQLRPHQTPALEAVKRGLTTADRGKLIMACGTGKTFTSLRIAEAVAGRGKTVL
                     FLVPSLALLSQTLDEWTQDTLIDLRCFAVCSDSDVGKKNHDDNVVVGISDLKYPATTNA
                     NSLVKAFNQPDIFGSDKPPYMNVVFSTYHSVEVIHQAQKLGFPAFDFIICDEAHRTTGA
                     TFEGDDESAFVRIHDNAYIAGQKRLYMTATPRIFGDDAKETEGVTLCSMDDKSLYGDDL
                     YVITFSKAVQLGILCDYKVIVLAVEEKHVSRRIQSLLKDENNQLKVDDAAKIVGCWKAL
                     AKQGLAGSDGVHPDSMKRAVAFCQVIEKDFRGKAHKVSSKLITEMFGAVVEAYQASEIE
                     HLRETAPDKAIDPSLKLQCQVKHVDGSMNATEKKSKIEWLKADTDENVCRILSNVRCLS
                     EGVDVPSLDAVLFLTPRSSQVDVVQSVGRVMRRAEGKELGYVILPVVIPAGEEPENALN
                     NNQAYRVVWQVLNALRAHDDRFDAMINKLEFNGKDTGRMEVIAVADKVVKKTKRQTKKS
                     ELAGKARKSSGIGSAVSPAPEQFDIEFSVGEIERALYAKIVKKCGNRHHWEDWANDIAK
                     IARTHIDRIHGILENPGNQVEISAFRAFADELRDDLNNSVSDDEIIEMLAQHLITKPVF
                     DALFADYNFTDHNPMSMAMQNVLNTLQEHHLEKEASTLNSFYESVKMRADGITTAEGKQ
                     QIIVQLYDKFFRNAFPRMTERLGIVYTPVEVVDFIIHSVNDVLKQEFGQTLADKGVHIL
                     DPFTGTGTFITRLLQSGLIPSDKLAYKFKNEIHANEIVLLAYYIAAINIEAVYHSITEE
                     SEYTPFEGICLTDTFEMYEKDDLVSEVLVDNSERRKRQKALDIRVIIGNPPYSSGQESA
                     NDNNQNVKYPMLDKRIAETYAACTTATNKNALYDSYIRAIRWASDRIGEQGVIGFVTNG
                     GYIDSNSADGLRKCLAEEFSSLYFFHLRGNARTSGEVRRKEKDNVFGQGTRTPIVIAIL
                     VKNPLATKMGKIYFHDIGDYLTREEKLEKISEFSSINGIEKSHGWQKITPDAYNDWLNQ
                     RDDSFYDFIEIGNKKSKDGHVLFLNYSRGAESGRDAWVYQSSKTKLTENVKGLLSFYNS
                     SLETDISDKMDPTKISWTSSLISSHQKMSKLTFDASCMRKSVYRPYQKQWLYFGSGLIH
                     RVGQIPQIFPTKDADNFLIYMSGSGNSGKDFSAIMTREIPDLNMQHSGGQGFPLYLYES
                     ISERKSAISDFGLDYFNDFYATNIISKEDIFYYVYGLLSSRDYLSRYADNLAKELPRIP
                     RVKKLEDFWSFSKAGRELAELHVNYETVDPYKADIDTGSLSYSQLGKDDFYVEKMKFAK
                     KGEKNTVIYNNKIRIKNIPIEAYDYIVNGKPALEWVMERQGVSTHKDSGIVNDANDWAI
                     ETMDNPRYPLELFLRVITVSLETQKIVNSMPKLDI"
                     /name="type ISP restriction/modification enzyme"
     gene            complement(2292..2885)
                     /locus_tag="QLG15_RS25365"
                     /old_locus_tag="QLG15_25375"
     CDS             complement(2292..2885)
                     /locus_tag="QLG15_RS25365"
                     /old_locus_tag="QLG15_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_016517639.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(2898..4007)
                     /gene="mobA"
                     /locus_tag="QLG15_RS25370"
                     /old_locus_tag="QLG15_25380"
     CDS             complement(2898..4007)
                     /gene="mobA"
                     /locus_tag="QLG15_RS25370"
                     /old_locus_tag="QLG15_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_061351404.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISRDGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQSILARREAERQAQQSRDERDCVIDVTTSLREAIHERDDASVKPAQQPEPEVA
                     AGITFDFEKEPDKLNALVSDAMKDVQEEIDLQSLVNDAMAEFQGIHQEMERQRERERLA
                     EKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            4172..4639
                     /gene="traD"
                     /locus_tag="QLG15_RS25375"
                     /old_locus_tag="QLG15_25385"
     CDS             4172..4639
                     /gene="traD"
                     /locus_tag="QLG15_RS25375"
                     /old_locus_tag="QLG15_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_106931013.1"
                     /translation="MAKSEKQEKWAADRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSSA"
                     /name="traD"
     gene            5234..6190
                     /locus_tag="QLG15_RS25380"
                     /old_locus_tag="QLG15_25390"
     CDS             5234..6190
                     /locus_tag="QLG15_RS25380"
                     /old_locus_tag="QLG15_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_039899550.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYSSFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDEFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELAKEPISKKEDNSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRATLEKILAKYGRN"
                     /name="replication initiation protein"
     gene            6198..6647
                     /locus_tag="QLG15_RS25385"
                     /old_locus_tag="QLG15_25395"
     CDS             6198..6647
                     /locus_tag="QLG15_RS25385"
                     /old_locus_tag="QLG15_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_016517644.1"
                     /translation="MALVSISEAARLTGKSRTTVQSYIKQGKLTKCTDSGGASKLDTSE
                     LLRVFGSFTGQQVGSEQSGSTVQHLAGDFVQLPDQNLLHENYALKAEIELVKALLREKE
                     LLLDEKDKRNEDLKHALLLIESKLPNTSEPVTPHVVKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     Typing          5235..6185
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.271"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2902..4007)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.702"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caatctcacc gactgaaaac tcaatatcaa actgttcagg agcgggactc acggctgaac
       61 caataccact gcttttacgc gcttttcctg ccagttcaga ttttttcgtc tgccgtttag
      121 ttttcttcac cactttatcg gctaccgcga tcacttccat tctgccggta tctttaccgt
      181 taaactccag cttgttgatc atggcatcaa agcggtcatc atgtgccctg agtgcattca
      241 gcacctgcca gacaacacgg taggcctgat tattgtttaa ggcattttcc ggctcttctc
      301 cggcgggaat gacaaccggc agaatgacgt agcccagctc ttttccttcg gcacgccgca
      361 tcacacgccc gacagactgc accacatcca cctgagagct tctcggcgtt aaaaacaaca
      421 ccgcatccag agaaggcaca tcgacacctt ccgataaaca gcggacgtta gacaatatcc
      481 ggcagacatt ttcatccgtg tccgctttca gccattcaat tttggatttt ttctcagtgg
      541 cattcatgct gccgtcaacg tgcttcacct gacactgaag ttttaatgac gggtcaatgg
      601 ccttatccgg tgcggtttcc ctgagatgtt cgatctcgga ggcctgatag gcttcaacta
      661 ctgccccgaa catttccgtg atcagtttag aactgacctt gtgtgccttc ccacgaaagt
      721 ctttttctat cacctgacag aacgcaacag cccgtttcat cgagtcaggg tgaacgccat
      781 cagatcccgc cagtccctgc tttgccagtg ctttccagca accgactatt tttgccgcat
      841 catcgacttt gagctgatta ttttcatcct tcagcagaga ctgaatgcgg cggctgacgt
      901 gtttttcctc cacggcaagc acaataactt tgtaatcaca cagaatgccc agctggacgg
      961 ctttggagaa ggtaatgaca tacaggtcat caccgtaaag gcttttgtca tccattgagc
     1021 ataaggtgac cccttcagtc tctttcgcat catccccgaa aatgcgcggg gtcgcggtca
     1081 tgtacaggcg tttttgaccg gcaatatagg cattgtcgtg aatacgaaca aaggcggact
     1141 catcatcccc ttcaaaggtc gcccccgtgg tccggtgggc ttcatcacaa atgatgaaat
     1201 caaatgcagg aaaacccagt ttttgcgcct gatggatgac ctcaacagag tggtaggtag
     1261 aaaacaccac gttcatataa ggcggcttat cactgccgaa gatatcaggc tgattaaacg
     1321 ctttgacgag gctgttcgca ttcgtggtcg ccggatattt caggtcgctg ataccaacaa
     1381 cgacattgtc atcatggttt ttcttaccca catcactgtc agagcagaca gcaaaacagc
     1441 gcaggtctat cagcgtatcc tgtgtccatt catccagcgt ctgactcaac aatgccagac
     1501 tgggcacaag gaacagtacg gttttccccc gtccggcaac cgcttcagcg atacgcagcg
     1561 aggtgaaggt tttccctgtg ccgcacgcca taataagctt gccgcggtcg gcagtcgtca
     1621 gccctctttt cacggcttca agcgcgggag tctgatgagg tcggagttgt ttttttgctt
     1681 tcatcaccgg tttgtaggcc ggatcaaaat cgaattgtga ccagtccagt gcgctttgct
     1741 caaggtctgt caggcttaac gcagtgacag gaacggcctt gtcccgcagc atatttttag
     1801 cttcctccgt ccagttatcc gtagaggcaa cgatatagcg gagcgtaaaa tacgttttat
     1861 cggaggccgc cagaaaacta tcaatatcct ttttggcgat tttagtctga ttgtagtttt
     1921 tgcactggat agcatgaaac tcccccgttt tggttacggc gaccaaatca atgccatcat
     1981 cttttttctt cgtgatccca acggtttcac cgtactgaga aacccatccg ccatagctca
     2041 gcacgtcact gtacagctcg gcatatttag gttcatggag gaaatactgc acacaaaggt
     2101 tttcaaaggt tgtacctttg tcccgctgtg ttggtgaagc ctgttcaatt tttgtcagca
     2161 atgcagaaag tgcattcatc gttgatatcc ttaatgtcct ttatttgctg gggttatcag
     2221 atccccccga cacgtttaat taatgctttc tccgccggag atcgacgcac agcgttctgt
     2281 gctctatgat gttatttctt aataatcatc caggtattct ctttatcccc tttatcacca
     2341 tacgtagtgc gagtgtccac cttaacgcag ggctttccgt cacagcgcga tatgtcagcc
     2401 agcggggctt tcttttgcca gaccgcttcc atcctctgca tttcagcaat ctggctatac
     2461 ccgtcattca taaaccacgt aaatgccgtc acgcaggaag ccaggacgaa gaatatcgtc
     2521 agtacaagat aaatcgcgga tttccacgta tagcgtgaca tctcacgacg catttcatgg
     2581 atcatcgctt tcgccgtatc ggcagcctga ttcagcgctt ctgtcgccgg tttctgctgt
     2641 gctaatccgg cttgtttcag ttctttctca acctgagtga gcgcggaact caccgatttc
     2701 ctgacggtgt cagtcatatt accggacgcg ctgtccagct cacgaatgac cctgctcagc
     2761 gtttcacttt gctgctgtaa ttgtgatgag gcggcctgaa actgttctgt cagagaagta
     2821 acacgctttt ccagcgcctg atgatgcccg ataagggcgg caatttgttt aatttcgtcg
     2881 ctcatacaaa atcctgtcta tcgtgagaat gaccagcctt tatccggctt ctgtcgtatc
     2941 tgttcggcga gtcgctgtcg ttctttctcc tgctgacgct gtttttccgc cagacgttcg
     3001 cgctctctct gcctttccat ctcctgatgt atcccctgga actccgccat cgcatcgtta
     3061 acaagggact gaagatcgat ttcttcctgt acgtccttca tggcatcact gaccagtgcg
     3121 ttcagcttgt caggctcttt ttcaaaatcg aaggtgatgc cggcagcgac ttccggctca
     3181 ggttgctgcg ccggcttaac ggacgcatca tcccgttcgt ggatggcttc ccgcagcgag
     3241 gtggtaacat caatgacgca gtcccgttca tcacgggact gctgcgcctg tctttccgct
     3301 tccctgcgcg caagaatgga ctgtaactgt tcagtatcga accgctgaac ctgacccgcg
     3361 cccagatgcc gctcaggctc acggtcaatg ccctgcgcct tcagggaacg ggaatcaacc
     3421 cggtcagcgt gctgataccg ttcaaggtgc ttattctgga ggtcagccca gcgttcccgc
     3481 tgggcaacaa ggtattcttt gcgttcggtc ggtgtttccc cgaaacgtgc cttttttgcg
     3541 ccaccgcgct ccggctcttt ggtgttagcc cgtttaaaat actgctcagg gtcacggtga
     3601 ataccgtcat taatgcgttc agagaacatg atatgggcgt ggggctgctc gccaccggct
     3661 atcgctgctt tcggattatg gatagcgaac tgataggcat ggcggtcgcc aatttcctgt
     3721 tggacaaaat cgcggacaag ctcaagacgt tgttcgggtt ttaactcacg cggcagggca
     3781 atctcgattt cacggtaggt acagccgttg gcacgttcag acgtgtcagc ggctttccag
     3841 aactcggacg gtttatgcgc tgcccacgca ggcatattgc cggactcctt atgctcaagg
     3901 tcggagtctt tttcacgggc atactttcca tcacgcgaaa tgtaatcggc atggggagag
     3961 gcactgcctt tcccgccggt ttttacgctg agatgataag atgccatcgt gttttatccc
     4021 gctgaaggcg cgcaccgttt ctgaacaaag tgaagaaacg tctaagtgcg cactgataat
     4081 aaaaagctta tcagtgataa tacctgcatt tgacctcggc cgccattcct gaccataatt
     4141 tccacatcaa cattcagagg ataaaacagt catggctaaa agcgaaaaac aggaaaaatg
     4201 ggcagcagac agagtgcagt acattcgcgg cttaaagtcg ccgaatgaac agcagaaact
     4261 catgctgata ctgacggata aagcggataa aacagcgcag gatatcaaaa cgctgtccct
     4321 gctgatgaag gctgaacagg cagcagaaaa agcgcaggaa gccagagcaa aagtcatgaa
     4381 tctgatacag gccgaaaaac gggcagaagc cagagccgcc cgtaaagccc gtgaccatgc
     4441 gctgtaccag tcagccggac tgcttatcct ggcgggtctg gttgacagcc agacggggaa
     4501 gcctgttgat gataccgctg ccttgctggg tgcattagcc agtctgaatg acctgtcgag
     4561 ggataatccg aagtggtcag actggaaaat cagaggtcag gaactgctga acagcaagaa
     4621 gtctgacagc tctgcatagc agacctgcca taaaacgccc tgagaagccc gtgacgggct
     4681 tttcttgtgt catgggtaga tttcctcgtg tgagtccatg aaaagcgcct gtagcatcat
     4741 ttacccccat tccctgccag agccgtgagc gtagcgaact gaatgtcacg aaaaagacag
     4801 cgactccggt gtctgatggt cggagacgaa aggaatattc agcgatttgc ccgagcttgc
     4861 gagggtgcta cctcattctt tcgtgtttga aggtatgttt tttggaggag caaacgctgt
     4921 ttgcgacatc cttttatcat atcgctcaac tggcactggc ggttagttat ccacaaagct
     4981 gggatctatt cttttttatc ttttttattc tttctttatt ctagtgattt taatattttg
     5041 ttttataagt aaaaaacaca tgaaaggtta agtgaaaatt aggaaaggtt aagcaaaaat
     5101 taggatcccc cttcaaaagg ttaagtgaaa attaggtaat ccacacaacc acataaaagg
     5161 ttatgacaat gaaattgaca taaccaagcg gaagggttat gttgttttaa aatcacaacc
     5221 aatggtttta gtcatggctg aattggtagt attcaaagca aacgagcttg cggttagtcg
     5281 ttatgaccta acagagcacg aaacgaaact aatcctgttt tgtgttgcaa agctaaaccc
     5341 tacattagaa acaccagatg aaactgaacg aacggttaaa ttttcatgct ccgaatattc
     5401 ttcatttatg gggataagtt atgagaatgc gtgggggcga ttaaatacat caacacgaga
     5461 gttatttaag cggtctattg aattaatata tccaacaggt gctgtatcta tgcgtgtttt
     5521 taattgggct gaatatgcag agttcaatag ggaaacacaa gaggtaactt tagtttttag
     5581 taaatatata atcccattac tatttcacct aaagagattc attaaataca acctcgagca
     5641 tgttaagtcc tttgaaaata aatactcaat gcgcatttat gaatggctgc taaaagaact
     5701 aacgcaaaga aaaacacata aagccaatat ttcaataagc attgatgagt ttaaattcat
     5761 gctaatgtta gaaaaaagct acccagaata taaattacta aatcattggg ttttaaaacc
     5821 aatttcaaaa gatttaaata cctatagcaa tataaaacta tctattgata agcgcggtcg
     5881 tcctgctgat acgctgatct tccaagttga actggataaa caaattgacc ttgtgactga
     5941 gctagcaaaa gaaccaatat caaaaaaaga agataactca atccgtttaa cgcctgaaaa
     6001 tcgtctgcat gaggggctaa aaacaacatt acatgatgct ttaactgcga aaatacaact
     6061 tactggattt gaagctaaat tcctgagtga catgcagagt aagtatgatc tcaacggctc
     6121 atttacatgg ctgactcaaa aacaacgagc cacgctagag aaaattttag ctaagtacgg
     6181 acggaactga ggtatctatg gctttagtat caataagcga agcagccaga ctaactggaa
     6241 aaagccgcac aacagtacag tcatacatta agcagggaaa actgaccaag tgcacagata
     6301 gcggtggtgc ttcaaaacta gacacatcag agcttctacg tgttttcgga tcatttactg
     6361 gtcagcaggt tggcagtgaa caatcaggca gtactgtgca gcatttggca ggcgattttg
     6421 tgcagttacc tgaccagaat ttgctacatg aaaactatgc cttaaaagct gaaatagaac
     6481 tcgtaaaggc attgctgcgt gagaaagagc tactactcga tgaaaaagac aaacgcaatg
     6541 aggatctcaa acatgctttg ctgctaatcg agtctaaact gccgaataca tcggaaccag
     6601 tgacacctca tgtggtgaaa aaatcatggc aattctggaa gaaatagcgt tttcagccgg
     6661 caaaccgact gaaaccggat ctgcgattct gataacaagt cggcaacacc aaaacagcca
     6721 gtttgcgagc ggaaaaaaac gcacttttgg acgttccggc gggtttcagg ggtgagtacc
     6781 aggtcagtgg tgcgcgcaag atccattatg ttaaaaacga caggatttat gaaaataacc
     6841 agaattgtgc aggatgtttt ttcatcctgc acacctagtg attagatatc tagttttggc
     6901 atgctattaa cgattttctg agtttcaaga ctgactgtga taactctcag gaaaagctct
     6961 agtgggtatc tagggttatc catagtttca attgcccagt cattagcatc gttcacaatc
     7021 ccgctatctt tatgggtaga aactccctgc cgctccatca cccactcgag agcaggcttg
     7081 ccattcacaa tgtagtcata ggcttcaata gggatgtttt ttatgcgaat tttattgtta
     7141 taaataacgg tatttttttc accttttttg gcgaacttca tcttctcaac ataaaaatcg
     7201 tctttaccta actgagaata actcagggat cccgtatcaa tatcagcttt atagggatca
     7261 actgtttcgt agtttacatg caactcagca agttcacgac ctgccttaga aaatgaccaa
     7321 aaatcttcta atttttttac tcgagggatg cgcggtaact ctttagctaa attatcagca
     7381 taacgagaca aataatccct actgcttaat agtccataaa cataataaaa aatatcttct
     7441 ttactaataa tattagtagc ataaaaatca ttgaagtaat ctaacccaaa atcactaata
     7501 gcgctttttc tctcactaat actttcatat aaatacaatg ggaaaccttg cccaccagag
     7561 tgctgcatat ttaagtctgg tatttctctt gtcattatag cggagaaatc ctttccacta
     7621 ttaccactcc cactcatgta aataagaaag ttatcagcat cttttgttgg gaaaatttga
     7681 gggatctgac caacacgatg aatcaaacca ctaccgaagt aaagccactg cttttgatat
     7741 ggacgatata ctgatttacg catacatgat gcatcaaaag ttaactttga cattttttga
     7801 tgactggata tcaagctact tgtccaacta atctttgttg gatccatttt gtctgaaata
     7861 tcagtctcta aggaagagtt ataaaaactt aatagacctt ttacgttctc agtcaatttt
     7921 gttttgctac tttgataaac ccatgcatca cggcctgatt cagcacctct ggaataatta
     7981 agaaagagaa catgcccatc cttagacttt ttattaccaa tctcaatgaa gtcataaaaa
     8041 ctgtcatcac gctgattcag ccaatcgttg taggcatctg gtgtgatttt ctgccaacca
     8101 tgtgattttt caatgccatt aatacttgag aattcggaaa ttttctccag tttttcttca
     8161 cgagtcaggt agtcaccaat atcatgaaaa taaatcttcc ccatctttgt tgctaacgga
     8221 ttttttacca gaatagcaat cacaatcggc gttcttgttc cctgaccaaa aacgttatct
     8281 ttttctttgc ggcgaacctc tcctgacgtt ctagcattcc ctctcagatg gaaaaaatac
     8341 aggctactga actcttccgc cagacactta cgaagtccat cagccgagtt agagtcgata
     8401 tagccaccat tagtgacaaa accgatcacc ccctgctctc cgatacggtc acttgcccaa
     8461 cggatagcgc gaatatagga gtcatagagt gcattcttgt tggtcgcagt ggtacaagcg
     8521 gcatacgtct ctgcaatccg cttatcgagc atcggatatt ttacattctg gttattatcg
     8581 ttcgccgatt cctgaccgga tgaataaggt ggatttccga taatcacccg aatatccagt
     8641 gccttctgcc gcttacggcg ttcactgtta tcaaccaaaa cctcactgac taggtcatct
     8701 ttctcataca tttcaaaggt gtcagtcaga caaatccctt caaatggcgt gtactctgat
     8761 tcttccgtga tgctgtgata caccgcctca atattaatgg cggcaatgta ataggctaac
     8821 aagacgattt cgttggcatg aatttcattt ttaaacttat aagccagttt atctgacggg
     8881 atcagaccac tttgcagcag gcgggtgatg aacgtaccgg ttccggtgaa cgggtcaaga
     8941 atatgcacac ctttgtcggc gagcgtttgt ccaaactcct gtttcagcac gtcattcacg
     9001 ctgtgaataa taaaatcaac cacctcaaca ggcgtataca caatgcccag gcgctctgtc
     9061 attcgcggga acgcgttcct gaagaactta tcgtacaact gtacgataat ctgctgctta
     9121 ccttcagctg tggtaatgcc gtctgcacgc atttttaccg actcatagaa gctgttcagc
     9181 gtgctggctt ctttttcaag atgatgctcc tgcaacgtgt taagcacgtt ctgcatcgcc
     9241 atactcatcg ggttatggtc ggtaaaattg taatccgcaa acagggcatc aaagaccggc
     9301 ttggttatca ggtgctgtgc cagcatttca atgatttcat cgtcggaaac actgttattc
     9361 agatcatcac gcagttcatc ggcaaatgcc ctgaaggcac tgatttctac ttgattaccc
     9421 ggattttcga gaataccgtg aatgcggtcg atatgcgtgc gggcaatttt agcgatatcg
     9481 ttcgcccagt cttcccagtg atgccggtta ccgcattttt taacgatttt cgcgtacagg
     9541 gcacgct
//
