LOCUS       NZ_CP124505             4071 bp    DNA     circular CON 10-JUN-2024
DEFINITION  Escherichia coli strain AVS0970 plasmid pAVS0970-C, complete
            sequence.
ACCESSION   NZ_CP124505
VERSION     NZ_CP124505.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34360954
            Assembly: GCF_030036795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Biggel,M.
  TITLE     Genomic characteristics of ESBL-producing Escherichia coli ST131
            isolated from the water ecosystem in a defined region in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030036795.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:15:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,112
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,742
            CDSs (with protein)               :: 4,742
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 112 of 263
            Pseudo Genes (incomplete)         :: 190 of 263
            Pseudo Genes (internal stop)      :: 52 of 263
            Pseudo Genes (multiple problems)  :: 78 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124505.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AVS0970"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pAVS0970-C"
                     /geo_loc_name="Switzerland: Kanton Zug"
                     /collection_date="2022-01-23"
                     /collected_by="Amt fuer Verbraucherschutz, Kanton Zug"
     gene            join(3609..4071,1..239)
                     /locus_tag="QJP73_RS25525"
                     /old_locus_tag="QJP73_25525"
     CDS             join(3609..4071,1..239)
                     /locus_tag="QJP73_RS25525"
                     /old_locus_tag="QJP73_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(496..2643)
                     /gene="mobQ"
                     /locus_tag="QJP73_RS25530"
                     /old_locus_tag="QJP73_25530"
     CDS             complement(496..2643)
                     /gene="mobQ"
                     /locus_tag="QJP73_RS25530"
                     /old_locus_tag="QJP73_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_021526521.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRENRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            2822..3076
                     /gene="mobC"
                     /locus_tag="QJP73_RS25535"
                     /old_locus_tag="QJP73_25535"
     CDS             2822..3076
                     /gene="mobC"
                     /locus_tag="QJP73_RS25535"
                     /old_locus_tag="QJP73_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2286..2644)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2645..2821
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..239
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.11e-121"
     Typing          complement(500..2730)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agttccgcag caccggtgac agaacgatag ctatcgatga tttccgaacc atgcttgccg
       61 ttgaagataa atatcaacaa ttcaaagact tgaataagat gataatcaag ccagccatcg
      121 ccgaactgaa cgaaaaaagc gatctggttg taactgttga gacgatcaaa aaggggcgca
      181 cggttgtagc cctgcatttt cgcttcaagg aagacaaaca gatcaagatg acgatctaaa
      241 atcgatttta aggcacctaa aacgctccct gctgcgtttt ttttgtgtta gtagtgcgaa
      301 ggtactattg aagtgaaaaa ttgcgtcaaa aacgctctga tgcagcgaaa aaagaccccc
      361 aatttttggg ggcggaaaaa caaaactatg cttgcgttat gtgagtgtgc ttcttgcatt
      421 catccctgtc gatgaacatc gaagaacatc gacgaacata acacgatgtt aaacaaaaca
      481 aaatattgtt cacattcact gtgaccaacc gcgatcatgt ctcctctcgg tggtgtgtgt
      541 tcgtgcatct tcctcgcgct gccattcctc ccaggtacga ttaaaaccca gcgagcggaa
      601 cagatgatcc ggctgttttt ctggtgtgtt gacggcagca aaaagcggtg cctcacgggt
      661 tttcagatga aactgcatca gttctggtgc aggtgatatg tggctgtctg catcgtgatt
      721 tacggcaaca ccataccctg cataccgtaa ccccttatcg gtcagcgcac tgatgaccag
      781 atgcctgtta tcggctgagg gaacggcaaa cgcgggatac agcgtaccat taagccggat
      841 aacaccgccc agagagtcat ccacgcccat gaccagcaaa agctgaagct gacggttttc
      901 ccggtcgaca gaactcatga ccggcatcag cgtcggtggc agacgtccct gcataaaatc
      961 caccttcctt gccgctggtg atggcatggc tggctggcgg tcttctccaa gacagaaaga
     1021 ctccggccat gtcttcggct ggcggagatt gcgggtatgt tgcggccatg tctggcaaac
     1081 ctgcgtcagc agttgctcaa aggttgcaga ggaataaaca cgttgcagtg gcggacgttg
     1141 gccgatttcg cttcggtcac agatataacc actcctgtca cgcattaaat caacactctc
     1201 aaagattctg gtgcggtatt tctccagctc gttcccccag agcgtatatt ctccgttccg
     1261 gttgtgaagc agaaggtaac aggtcatttc cccgttacca ggcatgggag cctcgccata
     1321 ccccgtaacg actgctctca gcacccctga atccataacg gcattgtcat ttttcctgag
     1381 ctgctccgcg cgactacgtt cagtatcttc ctgttccttc cggcgcaatt cctcttcacg
     1441 tttaagcaac tgggctctga gagctgcaag gccggattcc ccccactgct gccactgttt
     1501 ttcaaatgcc atctgactcg cgggaatgtc ctgtttctcc accttccggt aatgctgtcg
     1561 taatttttcc atttgctggc tcagagactg gatttttccc tgctcactac ggtattccgg
     1621 tgccgcccat tgcatcaggg gaatacggga aaaaccggac gaaaacggcg atgatttttt
     1681 caggctggca gcatggctca tcgcttcctt tcgttcggtg ttcagctctt cccagcgtct
     1741 gcgcgtcgcc tgcatatctg ccatcatcga catggcatta ctggtgagtt tccatgccga
     1801 gttactgtcc ttcggcaacg cttcgataat gtcacgtagt gtgtacgaag ttgcgttgtc
     1861 aggaagcccg atattcagaa ggtcagccac acggcgggca ggctctgtca gccctccgtt
     1921 cctgagttcg gtgcgggaaa tctgtatttc caggttaagc ctgttaatca gacgattctg
     1981 ttcgccgcgt tctgtttcga taccgcgtgt ttccatcgca caggcagact ttccgaggtg
     2041 aataccaggc gttttctcaa gcccctgtcg ctcgtatgaa cgatggtcta tctcttcctg
     2101 ataaccggcg ttcgccagtg ccctgtttgc atggtcagcc catgatgccc gccacgtctc
     2161 ggcatgtgtc cggtcgttcc agtccctgac ctttccgctg aacccagcag gtccgacagc
     2221 cctcgtggtc agcatgatgt gcgcatgggg atttgtttta tccatgtgat ggaacgccac
     2281 atcagcaatc atgccctgac tgacaaagtt ttctctgaca aaggcgagaa cggtttcccg
     2341 tgcggcatcc tgcggcagtt cgtgggggat ggcgagttca atttcccgcg caagttgtga
     2401 gttcttccgg cgttcggcct tctcaacggc attccagagt tcggcacggt tcgtacacca
     2461 ggcaggggcg gtggcggggg tcagaatcac agcctcagcc acaccgcttt tgcgggtgta
     2521 gtcgtgagtc agtccggtac gctcatcaag catcagtgaa ccggaacgat aggcagcgga
     2581 agctacggcg ctgtagccgc tgcttctgga gataattttc atgctgagat gataaatcgc
     2641 catttctgcc gtaatgcctt tttgtagttt tctttcagcc ccgcagggcg cacacaatcg
     2701 aaacggagtg gagattgtat aagtgcgcac ttcgtgattt gccgtgcatt atgccagctc
     2761 agtttttaaa ctaccagtga cgcgctaaaa tgcgattttt catcgcgcac ggagttacat
     2821 catgagcaga acgctcgaac agaagattgc tgatgctgag gcgagattac agcgcctgaa
     2881 ggcgaagagc cggagcctgg acacagcgca gaaggttgtt gtgggtgctg cattgctggc
     2941 aaaggtcaga aagccggagg aggtgcagtt gcgtgcctgg ttactccagt tcctgaaggc
     3001 agaggtgaca cgacaggctg atgtgacgcg catactgccg ctgattaacg agctggaggc
     3061 gctgccagaa cagtgattgt atcgtccgct cgccgcagcc ccagcgaccg agcgttagcg
     3121 agcgagtggg cgaggaagcg gaagtgaatc agtcgccagg ttgagcacat acgcacggtt
     3181 tttcggcagg tgatatgcgc tgaacccgca tgaatactgg gtttccacag ggttggaggg
     3241 ggtgtattcg aaacttgcaa ttctgcttta ttgcaacgca ttgtttttat tggttttttg
     3301 ttttcagcac cccaagttat ccacaggctc gccgttaagt ttttaagatc ttatttttag
     3361 atcttttttt taaaaccttt ttaggtcttg taacaccatg attttaaaga tgttctggca
     3421 agataagtgg agcttttggg ggtgaaaggt ggagcttttg ggggtgaaag gtggagcttt
     3481 tgggggtgaa aggtggagct tttgggggtg taatccacag aaaaaggggg ttgcaaaaaa
     3541 aaaacttcca cccatatgat aagtggcgta cagtaaaaaa acgccaccaa tagcaacgag
     3601 caagcgaaat gtcagatctg atagcttaca aatcaaacgc ccttatcgag gcaagctaca
     3661 agctcacttt gcaggaacag cgcttcctgc tggtctgtat cagccgcctg aaatccggag
     3721 aagattcccc ttcgccggat gaacaaaaaa ccatgaccat cacggctgca gagtttcatg
     3781 atgcctttcc tgacatggga cggcagcacg ctgaagctag gctcaaggaa gcgatagatc
     3841 gcctctggga caggtcagta atcatcaaga acgaggaaaa acgagaagag ttccgctggg
     3901 tgcagtaccg ggcgcagtat ttcaaaggtg aggcgaaagt agatatcacc ttctccgatg
     3961 ccatcatgcc gtatctgaca cagctcaagg ggcagtttac gcgggtagtc gtgaagaacg
     4021 tctccagcct ttccagcacc tacagcatcc gcatttacga gctgctccag c
//
