LOCUS       NZ_CP124504             7620 bp    DNA     linear   CON 10-JUN-2024
DEFINITION  Escherichia coli strain AVS0970 plasmid unnamed.
ACCESSION   NZ_CP124504
VERSION     NZ_CP124504.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34360954
            Assembly: GCF_030036795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Biggel,M.
  TITLE     Genomic characteristics of ESBL-producing Escherichia coli ST131
            isolated from the water ecosystem in a defined region in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030036795.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:15:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,112
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,742
            CDSs (with protein)               :: 4,742
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 112 of 263
            Pseudo Genes (incomplete)         :: 190 of 263
            Pseudo Genes (internal stop)      :: 52 of 263
            Pseudo Genes (multiple problems)  :: 78 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124504.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AVS0970"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="unnamed"
                     /geo_loc_name="Switzerland: Kanton Zug"
                     /collection_date="2022-01-23"
                     /collected_by="Amt fuer Verbraucherschutz, Kanton Zug"
     gene            complement(<1..174)
                     /locus_tag="QJP73_RS25465"
                     /old_locus_tag="QJP73_25465"
                     /pseudo=""
     CDS             complement(<1..174)
                     /locus_tag="QJP73_RS25465"
                     /old_locus_tag="QJP73_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /name="IS66 family transposase"
     gene            complement(214..561)
                     /gene="tnpB"
                     /locus_tag="QJP73_RS25470"
                     /old_locus_tag="QJP73_25470"
     CDS             complement(214..561)
                     /gene="tnpB"
                     /locus_tag="QJP73_RS25470"
                     /old_locus_tag="QJP73_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_000612632.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIFI
                     FRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDWRQ
                     PKRLLTSLTML"
                     /name="tnpB"
     gene            complement(558..938)
                     /locus_tag="QJP73_RS25475"
                     /old_locus_tag="QJP73_25475"
     CDS             complement(558..938)
                     /locus_tag="QJP73_RS25475"
                     /old_locus_tag="QJP73_25475"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_001171523.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGIN
                     ANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHG
                     TLRFNGNVSEKLLTLLIQELKR"
                     /name="transposase"
     gene            complement(1238..1687)
                     /locus_tag="QJP73_RS25480"
                     /old_locus_tag="QJP73_25480"
     CDS             complement(1238..1687)
                     /locus_tag="QJP73_RS25480"
                     /old_locus_tag="QJP73_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223216376.1"
                     /translation="MSAIASTPQTEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFK
                     KEYSIVSHMLVTDTITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRL
                     QDQQEETSVTLFQGKMSTDGNNYSIIKGKVSSVLLLLKMLQINHA"
                     /name="hypothetical protein"
     gene            complement(1705..>2595)
                     /locus_tag="QJP73_RS25485"
                     /old_locus_tag="QJP73_25485"
                     /pseudo=""
     CDS             complement(1705..>2595)
                     /locus_tag="QJP73_RS25485"
                     /old_locus_tag="QJP73_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /name="IS3-like element IS600 family transposase"
     gene            complement(2608..>3216)
                     /locus_tag="QJP73_RS25490"
                     /old_locus_tag="QJP73_25490"
                     /pseudo=""
     CDS             complement(2608..>3216)
                     /locus_tag="QJP73_RS25490"
                     /old_locus_tag="QJP73_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /name="IS30-like element IS30 family transposase"
     gene            3288..>3976
                     /locus_tag="QJP73_RS25495"
                     /old_locus_tag="QJP73_25495"
                     /pseudo=""
     CDS             3288..>3976
                     /locus_tag="QJP73_RS25495"
                     /old_locus_tag="QJP73_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc52 family transposase"
                     /name="IS3-like element ISEc52 family transposase"
     gene            4649..6157
                     /gene="ltrA"
                     /locus_tag="QJP73_RS25500"
                     /old_locus_tag="QJP73_25500"
     CDS             4649..6157
                     /gene="ltrA"
                     /locus_tag="QJP73_RS25500"
                     /old_locus_tag="QJP73_25500"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_001189123.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAHT
                     PGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALRDR
                     IVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEGDLS
                     SYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVISPLL
                     SNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCRYADDF
                     VLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIRKRSRYG
                     EMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQFVDFKAKV
                     FSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNHGKLSGEIL
                     YRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
                     /name="ltrA"
     gene            <6241..>6579
                     /locus_tag="QJP73_RS25505"
                     /old_locus_tag="QJP73_25505"
                     /pseudo=""
     CDS             <6241..>6579
                     /locus_tag="QJP73_RS25505"
                     /old_locus_tag="QJP73_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611570.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            6659..7063
                     /locus_tag="QJP73_RS25510"
                     /old_locus_tag="QJP73_25510"
     CDS             6659..7063
                     /locus_tag="QJP73_RS25510"
                     /old_locus_tag="QJP73_25510"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000839179.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGINDN
                     LLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLNIS
                     CEVTFRHGSLRLNGAISENILNLLIRELKR"
                     /name="transposase"
     gene            7060..7407
                     /gene="tnpB"
                     /locus_tag="QJP73_RS25515"
                     /old_locus_tag="QJP73_25515"
     CDS             7060..7407
                     /gene="tnpB"
                     /locus_tag="QJP73_RS25515"
                     /old_locus_tag="QJP73_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_000612626.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVFI
                     FRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDWRQ
                     PKRLLTSLTML"
                     /name="tnpB"
     gene            7456..>7620
                     /locus_tag="QJP73_RS25520"
                     /old_locus_tag="QJP73_25520"
                     /pseudo=""
     CDS             7456..>7620
                     /locus_tag="QJP73_RS25520"
                     /old_locus_tag="QJP73_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /name="IS66 family transposase"
     Typing          6239..6576
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.496"
                     /coverage="96.0"
                     /evalue="2.53e-58"
ORIGIN
        1 gcgactgccg aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc
       61 gcgctcccgg ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag
      121 gcgctgtttc agcaggaaga tgtcgtcaga aagatgtcgt tcataagccc gtattttacc
      181 gggcttattc tgtgacaacc aggataaaga gatttacagc atggtcaggg aggtcagcag
      241 ccgcttgggc tgtcgccagt cgataccttc cagcagcatc gccagctgcg cctgcgtaag
      301 gaacactttg ccatcacggg ctgacggcca ggcaaagcgc ccacgctcca gccgtttggt
      361 caggaggcac agtccgtcac cggtggacca cagcagttta acctgactgc cgctgcggcc
      421 ccggaaaatg aaaatatggc cggacatggg atcgtctttc agcgccgttt gtactttcgc
      481 agccaggccg ttgaagctat ttctcatatc ggtgataccg gcaaccagcc aaattttggt
      541 cccggaaggt aacgggatca tcgcttcagt tcctgtatca gcagagtcag gagcttttcg
      601 ctgacattgc cattgaagcg gagcgtcccg tgccggaacg ttacctcaca gctgatactg
      661 agggtttccg ggtcctctgc gagcgattct ggctgttcgg cagctgcatc gagagtcaca
      721 ggaagtagct gggggctctc tgaagaaggt aatagcagct ttccctcgcg ccattgttgt
      781 cgccatttga acaacagatt ggcgttaatg ccattttcaa gagcaagttt tgagatggat
      841 atcccgggtt cacaggaggc agcaacgagc agctgtttaa attcgggagg ataattaggg
      901 cagccttttc gcctgccggg agtcacattt ttctgcatat ctgacacttt ggttcccact
      961 acttatttgg tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct
     1021 gtacgcttac agttgttcag gagtaatagt ccaaagtatc gggaattttc cagacaatcc
     1081 tgataatacg cttatgaaca ttacaacacg ctcatgcgga acctggatgg gggaaggaac
     1141 ggtgaagctg ttagcgaatg actggcgttt gtataaacgc cggtcatgat gaatgatatt
     1201 tcagaatata cctcgggcta aaataccggg acagagatca ggcatgatta atctgcagca
     1261 tttttaataa cagaagtact gagcttactt tacctttgat gatggaatag ttatttccgt
     1321 cggtactcat ttttccctga aataatgtca cggatgtttc ttcctgctga tcctgcaggc
     1381 gcagtcgtaa ggtcatattt ttaatgagct gagatccggg tggcaagggg tatttgctgt
     1441 cagatactgg gcgactgagt ctgatgtggc gcgtatacag cccgttttcg tacagggtgg
     1501 cctctgttat ggtgtcagtg accagcatgt gggagacaat gctgtattct tttttaaagc
     1561 acccaccaga aacggtgatt gtaatgataa tggtcttctc gatcgagatg taaatacccg
     1621 tttcgttata ctgtccgtat cggaaccatg agaactcagt ttgaggtgta ctggcaatag
     1681 cggacactac catttgttct ttttttaagc agccatctga tgatattttt ccctgaaggc
     1741 tgccggggag atattcccca gacgagagtg acgacgctga cgattgtaga aaatctcaat
     1801 gtattcccgt attactgaga tggcttcatc ccggttatta aaacgatagt ggctcaggct
     1861 ctcatttttc agcgttcccc agaagctttc catcggagcg ttgtcgtaac agttaccttt
     1921 acgcgacatt gatgttttca gaccaaactg ctcctgtatg acccggtaat cgtatgcgca
     1981 gtactgtgaa cctcgatcag agtggtggat tagcccggca ggtgggcgct ggctcctgag
     2041 cgccataaac agggctttac ctgtcagctc ttttgtcatg cgctctccca tggcgtagcc
     2101 gacaatttcg cacgtataaa catctttgat gccagcgagg tacaaccatc cctcctgtgt
     2161 ggcaacatac gtcaggtccg ccacccagac ctgatttggt gctgtaggag cgaacgtctg
     2221 gttcagcaga tttggcgcaa ctggcagatt gtggttcgag ttcgtagtcg ctctgaactt
     2281 gcgtttctgc ttacagcgta gccttagctc cttacgaaga cgtgccagtc ggtcacgacc
     2341 aacgatgatg ccattctctg ccagctccgt ctggagccgc cgggttccat atgtttcgcg
     2401 agtgctgata tgtgccacct taatctccag ttttagccgc tcatcacttt gttttctgtc
     2461 tgaggggtca tgctgtaccc agttgtaata accgctcctg gatacaccaa atacctgaca
     2521 catcgcttca atgggaaatt gttgtcgcca ttgttcgatt aacgcgtatt tttcagcgac
     2581 tcctgtgcaa aatacgcggt tgtagattca atctgtcaat gcaacacccc tttcaattat
     2641 ctctttcggt gttttgaact tcagtgtctt tctcggtctg ttgtttagct gagcagcaac
     2701 cagatctagt tcatgttgag tatattgggc aagacatgtc tttttaggaa agtactgccg
     2761 aattagccca tttgtgttct catttgttcc ccgctgccaa ggactctgag gatcgcagaa
     2821 gtaaacttta acgccggtgc tgacagtaaa ttctagatgt ctggccagtt ccattcctct
     2881 gtcccatgtc agtgattttc tgagttctga cggtaaactc aggaatttgt cggtaagagc
     2941 ctgatttact gagacagaat ctttgcccct gagtctaagg atgatcgtat aacgtgattt
     3001 tcggtctaca agtgtggcta tatgagagtt ttttgtacct gagactaaat cgccctccca
     3061 atgtcccaga gagcgtctgt tatcgatatt tcgggaacgt tcgtgaattg gtgttccgtt
     3121 cactatgtta atcgtacctc tttcgccttt gcgggtatga cgcctgccat ggcgaaggct
     3181 atgcgaccgt cgcagatgct gtatattcag gtggttgaag tggtttactg aatttggcca
     3241 cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgccataatg aaaaaaagaa
     3301 atttcagcgc agagtttaaa cgcgaatccg ctcaactggt cgttgaccag aattacaccg
     3361 tggcagatgc agccagcgct atggatgtcg gcctttccac aatgacgcga tgggtgaaac
     3421 aattacgtga tgagcggcag ggaaaaacac caaaagcctc ccctattacc ccggaacaaa
     3481 ttgaaatccg tgagctcagg aaaaagctac aacgtattga aatggaaaat gaaatattaa
     3541 aaaaggctac cgcgctcttg atgtcagact ccctgaacag ttctcgataa tcgggaaact
     3601 cagggcgcgt tatcctgtgg ccactctctg ccatgtgttc ggggtccatc gcagcagcta
     3661 caaatactgg aaaaaccgtc ctgaaaagcc agacggcaga cgggctgtat tacgcagcca
     3721 ggtacttgaa ctgcatggca tcagccacgg ctctgccgga gcaagaagca tcgccacaat
     3781 ggcaacccag agaggctacc agatggggcg ctggcttgct ggcagactca tgaaagagct
     3841 ggggctggtc agctgtcagc agccgactta ccggtataaa cgtggtggtc atgaacatgt
     3901 tgctatccct aactaccttg aacggcagtt cgccgtgacc gagccaaatc aggtgtggtg
     3961 cggtgatgtg acctatagtg tgcccggagt tcagggcggg catggatgct taaatgaacc
     4021 gcgagtctgt ctggaatatt gaaccggtaa ctcacgatga gaaacccaac aatcccaccg
     4081 ggtgtgacgg tggagaacct gagcggcagt gacctgcggc atgcccgcag ggtgatgtaa
     4141 cccgctgaca acggggattg aggcgagatc actaagccga gatgatcctc aaggttaagt
     4201 actgaaaggc tgaagaacat gaacccgtta atccgcctct gtgggttgaa aacgtcacca
     4261 cggcctacgt gatctgacag gccgtgcagg aggaactggc agtgatacgt aagcactgcc
     4321 ggtcgaaggt gttttgacat gtatgcgaaa caccggggca gcagcgtcta tcacgcttgc
     4381 gttgctgact tctgccaact tgcggcaagc aaggataaag agtgcgacgg gcagcctcct
     4441 cagtatgcct gagtccaggc aggtaaaccg gggaaggtca gcgacggatg ttaagggggc
     4501 atggctccga tgacgcgctg gctggcggag cttccgtagt agtccgcgat ggggaaagcc
     4561 cattacatgg cgaagggaag cagtttgaat gtgtttgcga cgtgaattaa ctgacctaac
     4621 gaggtgaaga cctttgataa tcagcgaaat gcaacgcaag cttgccacat gggcagtcac
     4681 cgatccgtcc ctacggattc aacggctgct gcgtctgata acacaaccag aatggctggc
     4741 tgaagcggcg cggatcacgc tttcatcaaa gggggcccat acccccggcg ttgatggcgt
     4801 gaacaaaaca atgctacagg ccagactggc tgttgagctg caaatcctca gggatgaatt
     4861 actctcaggc cactaccagc ccttgcccgc cagacgggtt tacatcccta aaagcaacgg
     4921 caaactgcga ccactgggta tccccgcgtt gcgggatcgt attgttcagc gggccatgct
     4981 gatggcgatg gagccgatat gggagagtga ttttcatacg ctctcatatg gcttccggcc
     5041 tgagcgcagt gtccaccacg ctatccgcac ggtgaaatta cagctcacag actgtggtga
     5101 aacccgggga cgctgggtga ttgaaggcga cctgtccagt tacttcgaca ccgtacatca
     5161 tcgactgctg atgaaggctg tacgccgcag gatcagtgac gcacgtttca tgactctgct
     5221 gtggaaaacc atcaaggcgg gacatatcga tgtcggtctc tttcgggcgg ccagtgaagg
     5281 tgtgccacag ggcggtgtta tatcgccgct attatcgaac atcatgctga atgagttcga
     5341 tcaatacctg catgagcgct acctgagcgg gaaagccaga aaagatcggt ggtactggaa
     5401 taacagtatc caacggggcc gaagtacggc ggtcagagaa aactggcagt ggaaacccgc
     5461 ggtggcgtac tgccgctatg ccgatgattt tgtcctcatc gtcaaaggca ccaaagcaca
     5521 ggcggaagcc atcagggagg agtgtcgggg tgtgctcgaa ggcagtctga aactcaggct
     5581 gaacatggat aagactaaaa tcacccatgt taatgacggc tttatctttc tggggcacag
     5641 gatcattcgc aaacgcagtc gttatggcga gatgcgagtg gtctcaacga tcccgcagga
     5701 gaaagccaga aacttcgccg catcgctgac agcactgtta tcaggcaact acagtgaaag
     5761 caaagtcgat atggctgaac aactcaaccg aaaactgaaa ggctgggcca tgttctatca
     5821 gttcgttgat tttaaggcca aagtcttcag ttatatcgac cgtgtcgtgt tctggaagct
     5881 ggctcactgg ctggcccgca aataccgtac aggtatcgct tccctgatga ggtggtggtg
     5941 taaatcaccg aaaccgggtc agagcaaaac gtgggtttta tttggtaaaa ccaatcacgg
     6001 caagctcagc ggcgaaatac tgtaccggtt ggtggggcaa ggcaagaagc tgttccgctg
     6061 gcggctaccc gaaggtaatc cctatctgag gacggagacc agaaacacgt atacatcgcg
     6121 ctttacagaa gtggcaatgg cgttcgccag catttaaatg gagagccgga tgcgctgaaa
     6181 ggtgcacgtc cggttcgggg aggagaggca gggaaatagt ccgactacgc cctgcctctt
     6241 actctactca gcacatggca gggggtcgtc tatgtggcgt tcatcattga tgtgtttgcc
     6301 ggatacatcg tggggtggcg ggtctcatcg tctatggaaa cgacattcgt gctggatgct
     6361 ctggagcagg cgttatgggc ccgtcgaccg tccggcacag tccatcacag tgataaaggt
     6421 tctcagtatg tatcgctggc ctacacacag cggcttaagg aagccggatt actggcatca
     6481 acaggaagta ctggtgactc gtatgacaac gcgatggcgg agagcatcaa tggcctttac
     6541 aaagcggagg taatacaccg taagagctgg aaaaaccgta agcgtcgtct cagcaccgtc
     6601 tggcagatcc tgaaattcct gagagaatag tggacaccaa atatggtgga cgctatccat
     6661 gaaatcatta accgcagtgc gtaaaaaaag ccctaattat cccgttgagt tcaaaatcaa
     6721 aatggttgaa ctctcgcatc gaccagagat ctccgtagcg caactcgctc gtgagcatgg
     6781 gatcaacgat aatttgctgt tcaagtggcg ccagtactgg cgcgaaggaa aactacgtcc
     6841 tccttcaaca acagaaaaca acgtgcctga gctgctcccg ataactcttg atgccgaaga
     6901 tgttgtccct acaacctccc cccggtcaca acctgtagct gctgcgacac ctgaatcact
     6961 caatatcagc tgtgaagtga cgttccggca cggatcactc cgtctgaatg gtgccatcag
     7021 cgaaaatatc ctgaacctgc tgatacggga gctcaaacgt tgatcccatt accatcaggg
     7081 acaaagatct ggctggtcgc tggcatcacc gatatgagaa acggcttcaa cggcctggcg
     7141 gcaaaggtgc agacgacgct gaaagacgat ccgatgtcag gtcacgtttt tatcttccgt
     7201 gggcgtaatg gcagtcaggt aaagctcctc tggtctaccg gcgatggact gtgtctgctg
     7261 accaaacggc tggagcgcgg ccgcttcgcc tggccgtcag cccgggatgg caaagtgttc
     7321 ctcacaccgg cacagctggc gatgctcctt gaaggtatcg actggcggca gcctaaaaga
     7381 ctgcttacgt ccctgactat gttgtaagcc tctttatcct ggtcgacgct gaatgagcct
     7441 ggtaatatac ccggtatgag cagctcactt cctgacgata tcaatgcact gaaacgtctc
     7501 cttgccgaac aggaggcgct gaaccgtgcc ctgctggaaa agctgaacga gcgtgaacgc
     7561 gaaatagacc atctgcaggc gcagctggat aaactccgcc ggatgaactt cggcagtcgt
//
