LOCUS       NZ_CP124470             4287 bp    DNA     circular CON 10-JUN-2024
DEFINITION  Escherichia coli strain AVS0747 plasmid pAVS0747-C, complete
            sequence.
ACCESSION   NZ_CP124470
VERSION     NZ_CP124470.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34360965
            Assembly: GCF_030037615.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Biggel,M.
  TITLE     Genomic characteristics of ESBL-producing Escherichia coli ST131
            isolated from the water ecosystem in a defined region in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030037615.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:10:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,010
            CDSs (total)                      :: 4,905
            Genes (coding)                    :: 4,723
            CDSs (with protein)               :: 4,723
            Genes (RNA)                       :: 105
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 60 of 182
            Pseudo Genes (incomplete)         :: 131 of 182
            Pseudo Genes (internal stop)      :: 33 of 182
            Pseudo Genes (multiple problems)  :: 35 of 182
            Pseudo Genes (short protein)      :: 1 of 182
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124470.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AVS0747"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pAVS0747-C"
                     /geo_loc_name="Switzerland: Kanton Zug"
                     /collection_date="2021-08-16"
                     /collected_by="Amt fuer Verbraucherschutz, Kanton Zug"
     gene            329..523
                     /locus_tag="QJB08_RS25005"
                     /old_locus_tag="QJB08_25005"
     CDS             329..523
                     /locus_tag="QJB08_RS25005"
                     /old_locus_tag="QJB08_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041631.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1188..1319
                     /locus_tag="QJB08_RS25090"
     CDS             1188..1319
                     /locus_tag="QJB08_RS25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071791513.1"
                     /translation="MHEPPVQPDRCALSGNCRLESNPERHAKAPLAAAIGRGCTRNC"
                     /name="replication initiation protein"
     gene            1547..2557
                     /locus_tag="QJB08_RS25010"
                     /old_locus_tag="QJB08_25010"
     CDS             1547..2557
                     /locus_tag="QJB08_RS25010"
                     /old_locus_tag="QJB08_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001522447.1"
                     /translation="MEFQELENSACLYWPKELAERAASISAISPLIETQDEFLSILTIS
                     TNKPTSCFDAVRLCNKISPNLFVKHLMVLSDIGGERLHRFFKDLDKIYPDRIMEFNIGN
                     NSYSYQFNSNRAWTTKNLNVEKSRLLQPVSDFTREMLDVCMLILWGGNTINNTNLPTEI
                     ENNCILGNLIGNKEAIEQFVKERYIMVSRQTGGATANDLGHICEIFIKEKLYKLIDDNI
                     SLDGHHIDGVTHNDKDLTTFDIVAKNTITKKCVGIEISFQVTTNSTIERKAGLAKSRRD
                     LLNSIGHKIAYVIDGSGNFQRSNAIKTIINHSDMTVNFSDNGINSLATFICETLK"
                     /name="hypothetical protein"
     gene            2554..3801
                     /locus_tag="QJB08_RS25015"
                     /old_locus_tag="QJB08_25015"
     CDS             2554..3801
                     /locus_tag="QJB08_RS25015"
                     /old_locus_tag="QJB08_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001522448.1"
                     /translation="MIKFVDLFSGTGGIRLGFEQAMKEIGLSTKCVKSSEIDKKACETY
                     KLNFDEESYCDIHDVDIDESFDVLLAGFPCQAFSYAGKQLGFADTRGTLFFEVERIIKK
                     NKPKLCLLENVRGLTSHDKGRTFSTILKSLHDLGYHVEYRLLNTSNFGVPQNRVRIYII
                     ASLKKEVTLTIPNDLGAKDTHNYKPSKGGHDFYTESKVSDILEDSPDKSYDCSDWFKQR
                     LEDIFKGDLNKVHGIRLIDTRNGNSLHSWDLGLKGECSNDEIAFMNSLIANRRKHIFGT
                     HQDGKALSKAQIETFFKHSETTNIIKNLIKKGYLKEKNGLYNPVCGNMSFEVFKFLDPN
                     KVSITLTASDANRLGVYHNNRLRRLTPRECARLQGYPDSYILHPKDSMAYKQLGNAVSV
                     PVIKNIFLDIMKNNFI"
                     /name="DNA cytosine methyltransferase"
     Typing          complement(195..293)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.0"
                     /coverage="100.0"
                     /evalue="6.26e-40"
     Typing          complement(604..689)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          complement(4102..4287)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.257"
                     /coverage="89.0"
                     /evalue="4.5899999999999975e-81"
     Typing          704..1257
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.065"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atttgtcata actctctatt tataaagaaa aatcagcaaa aacttgtttt tgcgtggggt
       61 gtgggcattt caggcatgag aacagactcg cgtttttccg gagtatcagc ggaattattg
      121 attaagaaac actcgacgca gagcgaaatt ttatgcaaca gcggctgcac tgcacaggga
      181 ttatttatca gttgatatgc tgtgccatga tacgtcctaa agaaacagga caggatgtgc
      241 aattgtaata ccgtcgcccg caatgtctta caattaccac ctgctcaggg tccccccgac
      301 acccggaact gacggagaac aacggaaggt gaacaaacaa caacaaactg cgctgaatat
      361 ggcgcgattt atcaaaagcc agagcctgac actgcttgaa aaactggatg ctctggatgc
      421 cgacgaacag gcggccatgt gcgaacgact gcacgaactc gcagaagaac tccagaacag
      481 catccagatt cgctttgagg ccgaaagtga aacaggaaca taacgaagct gacggaggcg
      541 gtcacggctt tcccgtgggc gttttcgtca gcagacaagc ccgtcagggc gcgtcagggg
      601 gttttagcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
      661 ctggcttaac catgcggcat cagtgcagat tgtaagaaaa gagcgccata tgcggtgtga
      721 aatgccgcac agatgcgtaa ggagaaaatg cacgttccgg cgctcttccg cttcctcgct
      781 cactgactcg ctacgctcgg tcgttcggct gcggcgagcg gtgtcggctc actcaaaagc
      841 ggagatgcgg ttatccacag aatcagggga taacgccaaa agaaacatgt gagcaaaaaa
      901 cgagaacccg gaaaaggcca cgccgctggc gtttttccat aggctccgcc ccccctgacg
      961 agcatcacaa aaaccgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1021 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1081 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1141 gttggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1201 ccgttcagcc cgaccgctgc gccttatccg gtaactgtcg tcttgagtcc aacccggaaa
     1261 gacatgcaaa agcaccactg gcagcagcca ttggtagagg gtgtacaaga aattgctgat
     1321 acggagttct tgaagtgaag gcccgactgc ggctacactg aaaggacaga tttggtggca
     1381 gcggtcttgt acgccctgtt accttgggtt agaaagcctg agaaacttct aaccttcgaa
     1441 aaacccacgc ctgagaacgt gggttttttc gtttacgccc gtagagatta cccatcatag
     1501 gggtgtaact caaggtctat ttggatagtt ttaccaagga ataatcatgg agttccaaga
     1561 attagaaaat agcgcatgct tatactggcc caaagaactt gcagaaagag cagcatctat
     1621 tagcgccata agccctctta tagagaccca agatgaattt ttatcaatac taacaatatc
     1681 aacaaataag cctacatctt gttttgatgc tgtacggtta tgcaataaga tatcacccaa
     1741 cctatttgtc aagcacctaa tggttttgtc agatattgga ggcgaaagac ttcatcgctt
     1801 ttttaaagat ttagataaaa tctatcctga cagaataatg gagttcaata tagggaataa
     1861 ctcttactct taccaattca atagcaatag agcatggaca accaagaatt taaacgtgga
     1921 aaaatcacga cttttgcaac ctgtaagtga ttttacacga gaaatgcttg atgtttgcat
     1981 gctgatcctg tggggaggta acactataaa caatacaaac cttccaactg aaatagaaaa
     2041 taactgtatt ttgggaaatt taattgggaa caaagaagca atagaacaat ttgtaaaaga
     2101 aagatatata atggttagca gacaaacagg aggtgcaacg gcaaatgatc ttggacacat
     2161 atgcgagatc tttataaaag aaaaacttta taaacttatt gatgataata tatctctaga
     2221 tggacaccat atcgatggtg taactcataa cgataaagac cttacgactt ttgatattgt
     2281 agcaaaaaac acgataacaa aaaaatgtgt tggtattgaa ataagttttc aagttacaac
     2341 aaactcaaca atagaaagaa aagcaggatt agccaaaagc agaagagatt tactcaattc
     2401 aatagggcat aaaatagcat atgtgattga tgggtctggt aattttcagc gctcaaatgc
     2461 cataaaaaca ataattaacc atagtgacat gactgttaac ttttcagata atggtataaa
     2521 ttcactagca acattcatct gcgagacatt gaaatgatta aatttgttga ccttttttct
     2581 ggcacaggtg gtattagact tggttttgag caagccatga aagaaattgg attgtcaaca
     2641 aaatgtgtaa aaagctcaga aattgacaaa aaagcatgcg aaacatacaa acttaacttc
     2701 gacgaagaat catattgtga tatacatgat gttgatatag atgaaagctt cgatgtttta
     2761 ttggcaggat ttccttgcca agcattctct tatgctggaa aacaacttgg ttttgcagac
     2821 acccgaggta cattattttt tgaagtagaa agaattataa agaaaaacaa gcctaaattg
     2881 tgtcttttgg agaatgtacg tggattaact tcacatgata aaggaaggac attttcaaca
     2941 atattaaaat cccttcacga tttagggtat catgttgaat acagactatt aaatacaagt
     3001 aattttggcg tccctcaaaa tagggttaga atatatataa tagcctcatt aaaaaaagag
     3061 gtaacgctta ctattcctaa tgatcttgga gccaaagata cgcacaatta caaaccatcc
     3121 aaaggtggtc atgattttta tacagaatca aaagtttctg acatactaga ggatagtcca
     3181 gacaagtcat atgattgcag cgattggttc aagcaacgcc ttgaagatat ttttaaaggt
     3241 gacctgaata aagttcatgg aatcaggcta attgacacaa ggaacggcaa ctccttgcac
     3301 tcttgggatc ttgggctaaa aggcgagtgc tcaaatgatg aaatagcttt catgaattca
     3361 ttaattgcaa atcgtagaaa gcatattttt ggcacacacc aagacggcaa ggctttgtca
     3421 aaagcacaaa ttgaaacatt tttcaaacat agcgaaacca ccaacataat aaaaaatcta
     3481 attaaaaagg gttaccttaa agaaaaaaac ggactataca acccagtttg cggaaatatg
     3541 tcttttgaag tattcaaatt ccttgaccca aataaagtgt caattacact tactgcatct
     3601 gatgccaata gacttggagt ctatcacaac aatagactgc gacgccttac accaagagaa
     3661 tgtgcaaggc tacaaggtta cccagactca tatatcttac acccaaaaga tagcatggca
     3721 tataaacaat tggggaatgc tgtaagcgtt ccagtcatta aaaatatttt tcttgatata
     3781 atgaagaata acttcatata aactatatta ctagcaaaac tgctctatgg ttatttaatt
     3841 aaatttaaca gatttaccat aatgtcgcat tgcgcgcacc gctaaaacgc gctgaacgcg
     3901 attctgccgc gaacaccgca aaaagagatg ttctccccgg ggtaaaccgg aaaaatgcgt
     3961 caggatcgct ttcagctcac tgcatagcta tgcatgaaag tgaatgacga tcggtttggg
     4021 cgcatacagt gcccatgaga cacattttcg gacattttca gttcgtgaat ccctgactca
     4081 ttgctacata tctgaacaga cacccctggc agcgccccca ggggtgtcct gtaaaaaaac
     4141 acaccgcgcc gctagcagca cccctaatat aagatgatgt tttttatgaa aatagtcagt
     4201 accaccccta aaaagcggtg ttggcgcagc tacaacaccg cgccgacacc gcttttttaa
     4261 atatcacaaa gagagcaaga agaacta
//
