LOCUS       NZ_CP124464            12575 bp    DNA     circular CON 10-JUN-2024
DEFINITION  Escherichia coli strain AVS0947 plasmid pAVS0947-D, complete
            sequence.
ACCESSION   NZ_CP124464
VERSION     NZ_CP124464.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34360966
            Assembly: GCF_030037835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Biggel,M.
  TITLE     Genomic characteristics of ESBL-producing Escherichia coli ST131
            isolated from the water ecosystem in a defined region in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030037835.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:07:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,192
            CDSs (total)                      :: 5,086
            Genes (coding)                    :: 4,851
            CDSs (with protein)               :: 4,851
            Genes (RNA)                       :: 106
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 85 of 235
            Pseudo Genes (incomplete)         :: 171 of 235
            Pseudo Genes (internal stop)      :: 36 of 235
            Pseudo Genes (multiple problems)  :: 48 of 235
            Pseudo Genes (short protein)      :: 1 of 235
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124464.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AVS0947"
                     /isolation_source="environmental: river/lake"
                     /db_xref="taxon:562"
                     /plasmid="pAVS0947-D"
                     /geo_loc_name="Switzerland: Kanton Zug"
                     /collection_date="2022-01-13"
                     /collected_by="Amt fuer Verbraucherschutz, Kanton Zug"
     gene            complement(289..447)
                     /locus_tag="QJP79_RS26055"
     CDS             complement(289..447)
                     /locus_tag="QJP79_RS26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(1106..1297)
                     /locus_tag="QJP79_RS25830"
                     /old_locus_tag="QJP79_25830"
     CDS             complement(1106..1297)
                     /locus_tag="QJP79_RS25830"
                     /old_locus_tag="QJP79_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1362..1685
                     /locus_tag="QJP79_RS25835"
                     /old_locus_tag="QJP79_25835"
     CDS             1362..1685
                     /locus_tag="QJP79_RS25835"
                     /old_locus_tag="QJP79_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1894..>2325
                     /locus_tag="QJP79_RS25840"
                     /old_locus_tag="QJP79_25840"
                     /pseudo=""
     CDS             1894..>2325
                     /locus_tag="QJP79_RS25840"
                     /old_locus_tag="QJP79_25840"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2363..2881
                     /locus_tag="QJP79_RS25845"
                     /old_locus_tag="QJP79_25845"
     CDS             2363..2881
                     /locus_tag="QJP79_RS25845"
                     /old_locus_tag="QJP79_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            2888..3121
                     /locus_tag="QJP79_RS25850"
                     /old_locus_tag="QJP79_25850"
     CDS             2888..3121
                     /locus_tag="QJP79_RS25850"
                     /old_locus_tag="QJP79_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3832..4071
                     /locus_tag="QJP79_RS25855"
                     /old_locus_tag="QJP79_25855"
     CDS             3832..4071
                     /locus_tag="QJP79_RS25855"
                     /old_locus_tag="QJP79_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            4068..4430
                     /locus_tag="QJP79_RS25860"
                     /old_locus_tag="QJP79_25860"
     CDS             4068..4430
                     /locus_tag="QJP79_RS25860"
                     /old_locus_tag="QJP79_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(4455..5348)
                     /locus_tag="QJP79_RS25865"
                     /old_locus_tag="QJP79_25865"
     CDS             complement(4455..5348)
                     /locus_tag="QJP79_RS25865"
                     /old_locus_tag="QJP79_25865"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(5351..6943)
                     /locus_tag="QJP79_RS25870"
                     /old_locus_tag="QJP79_25870"
     CDS             complement(5351..6943)
                     /locus_tag="QJP79_RS25870"
                     /old_locus_tag="QJP79_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(6982..7680)
                     /locus_tag="QJP79_RS25875"
                     /old_locus_tag="QJP79_25875"
     CDS             complement(6982..7680)
                     /locus_tag="QJP79_RS25875"
                     /old_locus_tag="QJP79_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            7744..8441
                     /locus_tag="QJP79_RS25880"
                     /old_locus_tag="QJP79_25880"
     CDS             join(7744..7993,7993..8441)
                     /locus_tag="QJP79_RS25880"
                     /old_locus_tag="QJP79_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223216367.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(8755..11733)
                     /locus_tag="QJP79_RS25885"
                     /old_locus_tag="QJP79_25885"
     CDS             complement(8755..11733)
                     /locus_tag="QJP79_RS25885"
                     /old_locus_tag="QJP79_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935408.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnEc1 family transposase"
                     /protein_id="WP_172905819.1"
                     /translation="MARRQILSQSERESLLALPDDELTLTRMAYFSEHDLALINAHRKP
                     ASRFGFAVLLCYLKNVGFAPDKKTPPSDALLKPIANRLKLADGLWSSYINGRETTRREH
                     LTELYRYLGVKAFTNKIQQSCITHLLPMATRTDKGILLAEEMLAYLRQNKVMFPAIDVV
                     ERTCAEAMAGGDKIVFQTLNAPLTSAHREALDRLLESSDNQPSRLTWLLQPPGKINGKN
                     VLQHIDRLNSIESLALPEGIDRTIHQNRLLKLAREGRKMSSRDLTRFSAARRHAILVCV
                     LEDARATLTDEIIELHERILNSLFNKAKKTQAERLQQTGRLIQLKLKQYIDIGQALSEA
                     HDSGEDPWLAIEKILPWPEFIASLEETRHLARKNNFDPLHIITEKYSTLRKYAPRMLST
                     LQLVATPAAQALADALLVIKDMYKKQLRKVPATAPLEFVPESWRKVVITPTGIDRQYYE
                     FCALSELKGALRSGDIWVKGSRRYKNFDDYLIPEKDFDKLSPALPLPVSADYHEYITNR
                     MILLQSKLEEVNKMAAHGELPDVEISDKGVKISPLDNCVPLQVSPLSELIYSMLPRPKI
                     TEILDEVNSWTAFTRHFSHIKNDITRPDTRLLLTTILADGINLGLTKMAEACPGCTKSS
                     LEDIQAWYIRDETYSAALAELVNAQGKRPLAAFWGDGTTSSSDGQNFRTGNSGRYAGQI
                     NPKYGQEPGCQFYTHISDQYSPFYTCIISRVRDSTHVLDGLLYHESDLDIREHYTDTAG
                     FTDHVFALMHLLGFAFCPRIRDLHDKKLFIKGKADQYPALHSLISPTRINLKEIEIHWR
                     EVLRLATSIKQGTVTASLMLRKLASYPKQNGLAKALREIGRIERTLFMLDWFRDPALRR
                     RVQAGLNKGEARNALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMER
                     AVDALKRKGMKINAQLLSHLSPLGWEHINLTGDYIWKNNRIPTSGKFRRLRSVKIDKVK
                     KQP"
                     /name="Tn3-like element TnEc1 family transposase"
     gene            11888..12529
                     /locus_tag="QJP79_RS25890"
                     /old_locus_tag="QJP79_25890"
     CDS             11888..12529
                     /locus_tag="QJP79_RS25890"
                     /old_locus_tag="QJP79_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001473476.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQERGHKIASYYRENISGT
                     KLDRPELGRLLMDSHRNDILLVEQIDRLTRLSNSDWITLKKQIEQHELRIVSLDVPTSW
                     QALSDKDPSQSDPITRAVITAINNMLIDLMAAMSHKDWLSRRQRQKQGIERAHTLGKYR
                     GKQADQERHQKVLYYRQVKKLSIRETADATGYSTSQVCRIQALYREPTTD"
                     /name="recombinase family protein"
     Typing          951..1030
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          complement(376..918)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1330..1679
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.48e-67"
     Typing          1676..3225
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taattcaccg agtgtaaagg ctgtacgaaa tgtgttgcag tccgataata gccccctcca
       61 ttgacgtttt acacatgtgt tgtaggttcc aggagtgggc ggatctcaaa aagatcaaag
      121 gatcttcttg agatcctttt tttctgcgcg taatctgctg ccagtaaacg aaaaaaccgc
      181 ctggggaggc ggtttagtcg aaggttaagt cagttgggga ctgcttaacc gggtaactgg
      241 cttggtggag cacagatacc aaatactgtc cttctagtgt agccgcagtt aggccaccac
      301 ttcaagaact cttaatatct caatccacct tgtccagtta ccagtggctg ctgccagtgg
      361 cgctttgtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      421 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      481 gccgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      541 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      601 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      661 atttttatga tgctcgtcag gggggcggag cctatggaaa aacgccaacg gcgcagcctt
      721 ttcctggttc tcgttttttg ctcacatgtt tcttttggcg ttatcccctg attctgtgga
      781 taaccgcatc tccgcttttg agtgagcaga caccgctcgc cgcagccgaa cgaccgagtg
      841 tagcgagtca gtgagcgagg aagcggaaga gcgccggaac gtgcattttc tccttacgca
      901 tctgtgcggc atttcacatc ggacatggtg cgctttccat acaattcgta ctgatgccgc
      961 atagttaagc cagtatacac tccgctatcg ctacgtgact ggttcagggc ttcgccccga
     1021 aaccccctga cgcgccctga ggggcttgtc tgctcccggc atccgctcac agacaagctg
     1081 ttaccgtctc cgggagctgt atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga
     1141 ggcagctgcg gtaaagctcg tcggcgtggt cgtgaagtga ttcacaaata tcggcctgtt
     1201 catctgcgtc cagttcgttg agtttctcca gcagcgttaa tgtctggctt ctgataaaac
     1261 gggccatgtt aagggcggtt ttttcctgtt tagtcactga tgcctccgtg taagggggat
     1321 ttctgtttta tggggtaatg ataccgatga aacgcgagag gatgctcacc atacgggtta
     1381 ctgatgatga acatgcccgg ttactggaac gctgtgaggg taaacagctg gcggtatgga
     1441 tgcggcgggt ctgcctgggt gagccggttg cacgctccgg gaagctgccg acactggcac
     1501 cgccgttact gcgtcagctg gccgccatcg ggaataacct gaatcagaca gcgaggaagg
     1561 tgaacagcgg gcagtggtct tccggtgatc gggttcaggt gttggctgcg ctgatggcca
     1621 tcggggagga gctgcgtcgg ctgcgtctgg ccgtcaggga acagggcgcg cgggatgatt
     1681 gttaaatttc atgccagggg aaaaggcggt ggcagtggtc cggttgatta cctgctggga
     1741 agggagcgaa accgggaagg ggcaacggtg cttcagggta atccggaaga agtccgggaa
     1801 ctcatcgatg ccacgccatt tgcgaagaaa tacacgtcag gtgttctgtc gttcgcggag
     1861 aaggagctgc cgccgggagg acgtgaaaag gtgatggcga gctttgagcg tgtactgatg
     1921 cccggtctcg aaaagaatca gtacagcatc ctgtgggtgg agcaccagga caagggacgg
     1981 cttgagctga attttgtcat tccgaacatg gagctacaga ccggaaaacg cctccagccg
     2041 tactacgacc gggcagacag gccgagaatt gatgcctggc agacgctggt gaatcaccat
     2101 tacgggctgc atgaccccaa cgccccggag aaccgccgga tactgactct ccctgataac
     2161 ctgccggaaa cgaaacaggc gcttgcagag agcgtcacac gcgggataga cgctctttat
     2221 catgtcgggg agataaaagg tcgtcaggat gtgattcagg cgctcacaga ggcagggctt
     2281 gaagtggtca gggtgatgcg aagcagtatc agcattgcag acccgaacgg tgggaagaat
     2341 atcaggctga aaggagcatt ttatgagcaa tcttttacag acgggcgcgg agttcgagaa
     2401 aaagctgaaa gagagagccg aatctacaga gacaatgctg aacgacgagt tcagcaagct
     2461 cgaaaaatct gtaagcaagg ctgtgacatc aaacgagacg aaaatcaaag acgctatagc
     2521 cctgttcaca gcttcgacag aggaatcact gaaaaaacac cgggaagggg tgaaagaggc
     2581 gatgatgcag cacaggaagg acgtgttaaa gctggcaggg aatacgggca tgatgttact
     2641 ggggatggtc cttttcctgt ttaccgtgag tggcgggacg ctctggtatc ttggcgggat
     2701 gatacaggcg aacctggaag aaatcaggat acaggaagaa acattgcaga aactgaacgc
     2761 gaagacatgg ggcgtggagt ttgtgcagga cgggagaagg aaatttctgg ttatccctca
     2821 ggggaaatcg gcaacggtga ttccctatca ggggaaggac tgggtacaac tgacggagtg
     2881 acacagagtg acggagctgg aaagacattt gctgaacgcc ttagagcaac tgcaacagga
     2941 ctatatgcag cggctgaacg aatgggagag cgccttcgtg gaattgcaga aaatgttttc
     3001 gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt
     3061 gcagcacttg agcgagcaga cagaacgctt gagccagtta tacagcgaga acaggagata
     3121 agggatgaac ggctgataca ggaacgcgaa catgagttat ccctggagcg tgagcgccag
     3181 ctggaaatac aggaacggac acaggatggc ccttcgctgg gatggtaatc agagacaggc
     3241 taaaatgttg tcagcgcgtt tccggtgcat tttttgggat aatggttggt tcgggggcac
     3301 cgggaacgcg cttcagtcag accttgctga aaacacagga acgcgttaaa acgcatacgg
     3361 gcgcgattct ggtgtgtctt ttgttgctgt gttttgcctc tattgccaga tcgcgctcat
     3421 tcccctgaac ttctgttttc ccgctccggt ccccgtcttc ttcgcttccg aaagcatgaa
     3481 aaaaatccgt ttcagctgtc tctggctggc aattcctgcg tgattcacat ggctgcatag
     3541 ctatgcatgt ggttaaaatt taccgggtgc gatcgcggca gtttttcggg tggtttgttg
     3601 ccatttttac ctgtctgctg ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaata
     3661 agggattatg gtaaattatc ttgttcctta gagaatgatt tgaggtggta tcctgcactt
     3721 atttaaatat gcttgcttgg gaaattccag gttgcagtat ggttgagttg taattttcct
     3781 atgataggaa aaatgttata ttattaattt atttgtgagg cttcataaag aatgaaaatt
     3841 tctgatattg aaaaaaacat atataaagga ctagctgaat ttatttcaca aaaaatcaga
     3901 gatgaacatg atatatgcct tggacagttt gaatcagagg aattacttga tgagattctt
     3961 aaaagagtaa tcccagtcat atataatgtg gctattgatg atgctataaa atcagtaagg
     4021 aatgttgggg atagattaga ggaggaatta gatatgagga aggtagtgtg actaatttta
     4081 aatcagactt aaatctaata agaaaagaat ggtataatgc attttattca ggtaatactg
     4141 aaatgctaga ttacttggaa gctgattggt ttatttcaac aaatggtcaa aaagttatct
     4201 ataaaaaaca tcagttacga aagattgata tgcgtccagc gaggatttta cctagcatag
     4261 aaagatatga atatgatgtc gtaatgcgtg agtttaaagg cattgcttgt gtttcaggga
     4321 aagctgaaat aaagcaaaat gatggaaata tattaatggc ttttattgag aattggataa
     4381 aggttaatgg tggatggaaa atgcagttta tatcgtatga aaaaatctaa ggggaagccc
     4441 ccttagatta atggttatat atgttcaagt tttatttttt tatagcgata agttctgctt
     4501 tgagctttaa gcatgttttc tgcaaactga atatcaaaac tatttatagc attagttatt
     4561 atagttttta ttttttcttt atcagataat gaatcaccat gctctatagg ttcccgcata
     4621 tatggattgt tttttaattc atcaaataat gttttaaata gttctaatcg acgaggtctt
     4681 ataactgtaa aagatatatg aagagagtgt ttgctctgag tcgtaacatt gtgccagtat
     4741 ccagcaggaa gatatatggc ctgacctggg gttatatcaa actcataatc tggggaggta
     4801 ttaggtggta gataatcgaa gcttctttca tcttcgagag ggtatgaata ggtagggttg
     4861 taaactttcc atttttttat tccctcaatt tgaacagcta ttacatcatg attgtcaaaa
     4921 tgaagcccaa agcttggtgt tgctgtaaat gcagcatata gattagcact acatgtacat
     4981 tctaactctt tagataacca tagtctgctt tcatctacag attcaaagaa tgactggcat
     5041 cgatctacta taagggttgc gccatcacgt aagcatttat atagctgatg gcgatttata
     5101 tgtggtgttc tatcgcctgt ttggctatat gtgtatctta taaatccttt atacccccta
     5161 atttcgggaa agttgtcatt tgccattcta actctaggat agtgcaatat atctttttca
     5221 agcaatttat taagatcatt ccagttgatt aagtttttat aattaacatc cgtgaataca
     5281 aacggatatt tctggaaatt atatttctta aatttttcta tgatttcctt ttttataaga
     5341 ttattcattt ttaaacctca tgttttgtga tatctataat ctgtgcttta ggtatattat
     5401 ttataagttg ttcatcatga gatataacaa tgatatgctt ttccttgttt tcctctttta
     5461 tataatcact gattattttt ataccaagtg aatcaaaccc ttttgtcggt tcatcgaaaa
     5521 taatcaagtc tacgtttttt ccagatgtaa gagatactcc taatttttgt ttttgtccac
     5581 cagataaatt atgtccgtaa cctttgatct cataatctag tggtttgtcg aacttaaatc
     5641 tgtgtagcag ctttttaatt gtatttaaat tattaatgcc ggaataatgt aatatatttt
     5701 cctctatagt tccaggcatg agatctagat cttgaggaac gtataagaca ttaacaaaag
     5761 gagaatttgc tttttcttgt tgtatcccat tgatttttat tgtgttttct tctgttgtta
     5821 tattgccaga gagaattttt gcaatagttg tttttcccga accattctca ccggttataa
     5881 aggtaacaac gccttttgtt aatattaatt tatggttgaa tgatttgttg tatatttcaa
     5941 tattgcaaat attgtctttt acatgaagat attgcttgtc tgtgtaattt gtttttttta
     6001 tgtcgtttat ttcggcacag tataaatcaa tttctgttct tgccattttt atttgtctaa
     6061 atagataacc aatgcgattt aatgggagaa aaacttggaa taaaaaagtg gcaagcataa
     6121 taatactacc acggcttgct ctaccatgaa gaaacatcca gtatgctgat aggataatca
     6181 gactactaaa aatgactgaa agaaaagcgg agctgatacc aaacaggaaa aattcattat
     6241 ttacttttct ttgtttttct gaatgtgtat ttacagcttg aattactcgt ttacgacttt
     6301 tatccgtggt atggaaagct ttatccatag gatacatgtg catcatatca aacattcttc
     6361 cagatgcgaa tttggagcta agtgcaactg atttcatcat tggaaatcta cgttgtgtca
     6421 tttttatgac aaatattata tagattgcca ttattattat aaactcaatg gctattaatg
     6481 tattaacact tatacaaatt gttatgatta ttccaatcat ttgtattaat aatggtacta
     6541 catcagaaca tattgtataa aataatgatg atgcagaaga aagcatgctt tctataacct
     6601 tatttagttc tcctttattt ttctttaaaa aatgctcttg gtgagaattt tgtatattat
     6661 caataacttg ctctctaagc tttacagata attctatatt tgttttttga gtcaagtatt
     6721 ccgatatgct atatccagca ggcataagga atctaattga taagaaacat gctataaata
     6781 ttaataatga gtttatttct cctattgaat ctttattgtc aataactgtt tttattaaat
     6841 atccgaatag aacgaaaaga aaactactta tagttgataa taatataata aaaaacaaat
     6901 tacatttatt aaatgtgatg atgtactttt caataaaatt caatttataa tccttaaagc
     6961 ccgtatatta cgggctttat gttatctaga attacatgag cacccagcgc cacatccttt
     7021 accgctgcaa ccacaaacag aaccattatc attccaaaca tcttttcgca ttattatttt
     7081 tacaaattct gcggctggaa ggtgagtgat actctttact ttttcgatgt aagaatcact
     7141 ggatatggat tcttttggaa ttcttatagt attaatgttc tttatgctgt atcttacagc
     7201 ataatcacca taccactttg cgacagtttc atgacctagg taaaaagcga ttttttcaat
     7261 ttgattttta tattcgttaa aacgatattc ttggaatgtt ataccatgct cattaacatg
     7321 ccttatctca ttttttagca tgtcatttgt atttggataa tggtggaggt aagcaccaag
     7381 atatttttca caaaagctgg tataagcatc tgtaaattga ataaacgtat gccacatttc
     7441 atctataatt tgtaattgct cgtgaacttt tatattgtag cattccgtta ggcatgttga
     7501 ggctagccag atgaatttct ttgtttcatt aaagatatca tcagcttctt ctggtgttac
     7561 attccattct ttacgaaatt ttagaagaac atcatcattg tgataactca tgatatcttc
     7621 taatgagcta acattatgaa cttttgcata agaactgctg ttcatcttat gttgtagcat
     7681 aacaccccgg taatggtgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
     7741 cccgtggctt ccatctccat cagttgtccc tcctgctcag ctactgaagg cgtggtgcgt
     7801 aacggtaaaa gtactgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg
     7861 caactgcagt tcacttacac cgcttctcaa cccggtacac accagaaaat cattgatatg
     7921 gccatgaatg gtgttggatg ccgggcaacc gcccgcatta tgggcgttgg cctcaacacg
     7981 attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca
     8041 gtgatgtgat tgtctgcgct gaaatggacg aacagtgggg ctacgtcggt gctaaatcac
     8101 gtcagcgctg gctgttttac gcgtatgaca ggatacggag gacggttgtg gcgcacgtct
     8161 tcggtgaacg cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg
     8221 tcgtggtatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc
     8281 acgtaatcag caagcgttac actcagcgca ttgagcgaca taacctgaat ctgagacaac
     8341 atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag ctgcatgaca
     8401 aagtcatcgg gcattatctg aacataaaac actatcagta agttggagtc attacctaac
     8461 acccccaatt atatatttga atgtagatga gaatagttat catttgttat ggtattgtta
     8521 aatttgtgct tgtgtcaata tgcctaattc tataaatgag atagtaatca agttttttaa
     8581 tggttatagg atccattagt aatacaaggt ttttgcagcc agtttttacg ggtaggggct
     8641 gcaaggaaaa caaaattttc ccactctaag agaagagaat tgagttggtt gaatgagtgg
     8701 atgcatctag ggccgctaat tggggtcagt ttggatatcg aaaattattg tacgttaagg
     8761 ttgttttttg accttgtcga tcttaactga tctcaaacga cgaaacttac ctgaagtcgg
     8821 tatccggttg tttttccaga tataatcgcc tgtcagattg atatgttccc aacctaatgg
     8881 tgataaatgc gataatagtt gagcatttat tttcatgccc ttacgcttca aagcatcaac
     8941 cgctctttcc atgtaaactg tgttccataa ggaaatagcc gccgtcaata aagtcaatcc
     9001 actggcgcga taactttgat tttccggttt ccgatctcta atctccccca atcgatgcat
     9061 gaatacagca cgggccagag cattacgggc ttctcctttg ttcagcccgg cttggacacg
     9121 tcgccgcaat gccggatccc gaaaccagtc cagcataaac agtgttcgtt ctatacggcc
     9181 aatctctctc agagctttag ccaacccatt ttgtttgggg taactggcaa gttttctcag
     9241 catcagggat gctgtcactg ttccctgttt tattgaggtc gccagacgta gcacttcacg
     9301 ccagtgaatt tctatttctt tcagattaat gcgggtcgga gatatcagag aatgaagtgc
     9361 cggatattga tccgctttac ctttaatgaa aagtttttta tcgtgaaggt cccgaatccg
     9421 gggacaaaat gcaaaaccca acagatgcat cagagcaaaa acatgatcgg tgaaacccgc
     9481 tgtatctgtg taatgttccc tgatatccag gtcgctttcg tggtacaaca atccatcaag
     9541 cacatgtgtt gaatctctga cccgactgat tatgcaggtg taaaacggac tgtattgatc
     9601 tgatatatgt gtataaaact ggcatccagg ctcttgccca tatttcgggt taatctgccc
     9661 agcataacga ccagaattac cagttctgaa gttctgccca tctgatgatg aagttgttcc
     9721 gtctccccag aatgctgcca gaggtctttt tccctgagca ttcaccagct ctgcaagggc
     9781 tgcggaataa gtttcatcac ggatatacca ggcctgaata tcttcgagtg atgatttagt
     9841 gcatccggga caggcctctg ccattttcgt caggccaaga ttgatgccat cagcaagaat
     9901 tgtggtaaga agcaggcgtg tatcgggacg agttatatca ttttttatat gtgaaaaatg
     9961 tcgggtaaac gctgtccagc tgtttacctc gtcgagaatt tctgtgattt taggacgcgg
    10021 taacatactg taaattaact ctgaaagcgg tgagacctgc aaaggaacac aattatccag
    10081 aggggagatt tttactcctt tgtcagatat ttccacatcc ggcaattcac cgtgtgcggc
    10141 cattttgttg acctcttcca gttttgactg aagcagtatc attcgatttg taatatattc
    10201 atgataatca gcagaaacag gtagtggtaa tgcaggtgag agcttatcaa agtctttctc
    10261 tggaataagg taatcgtcga aatttttgta acgtcgtgag cctttaaccc agatatcccc
    10321 ggaacgtaac gcccctttca gctcactaag tgcacaaaat tcatagtatt gtcggtcaat
    10381 accagtggga gttatcacca cttttcgcca gctttcagga acaaactcaa gcggagctgt
    10441 cgccgggact tttcgtaact gtttcttgta catatctttg attacaagca acgcatcagc
    10501 cagcgcctgt gctgccggtg tggcaactaa ttgcaaggtg gataacatac gcggagcata
    10561 tttccgtaat gtgctgtatt tttcggtaat aatatgtagc gggtcaaaat tattctttct
    10621 ggcaagatga cgagtttctt ccaggctggc aataaattca ggccatggca atattttctc
    10681 tatagcaagc catggatctt caccagaatc atgggcctca gataaagcct gtccgatatc
    10741 aatatactgc tttaatttta actggattaa ccttcctgtt tgttgaagac gttcagcctg
    10801 tgtttttttg gccttgttga acagactgtt caatattcgt tcatgcaact caataatttc
    10861 atctgtcagc gtagctctgg catcttccag gacacacacc agaattgcat gacgtcgggc
    10921 tgctgaaaat cgagtcaggt ccctactgct catctttcgc ccttctcgcg caagtttcag
    10981 taacctgttt tgatgaatgg tacgatcaat accttccggc aatgccagtg attcaatact
    11041 gttcagccga tcaatgtgtt gcagtacatt cttaccgttg attttacctg gcggttgcag
    11101 aagccaggtc agccgggaag gttgattgtc tgatgattca agcaaacgat ccaaagcttc
    11161 cctgtgagca gaggttaagg gcgcatttag cgtctgaaag actattttat cgccgccagc
    11221 catagcttcc gcacaggtac gttccacaac atcaatcgca ggaaacataa ccttgttttg
    11281 ccgaaggtag gccagcattt cttccgccag gagaatgcct ttgtcagtac gagtcgccat
    11341 tggcaacaga tgtgtaatac aactctgctg tattttgttg gtgaatgctt ttacaccaag
    11401 ataacggtac aactcggtta aatgctcgcg tcgagtagtc tccctgccgt tgatataaga
    11461 ggaccatagc ccatcggcaa gttttaacct gttggctata ggttttaaca atgcatcaga
    11521 ggggggcgtt ttcttatcag gagcaaaacc gacatttttc agatagcaaa gaagaactgc
    11581 aaatccaaaa cgactggctg gtttgcgatg agcattaata agagccaggt catgttcgct
    11641 gaaatatgcc attcgggtca gggtcaattc atcatccggc aatgccagta gagattctct
    11701 ttccgactgc gaaagaatct gcctccgtgc catattcata cctcacatag tacttttaac
    11761 gaaacaggtt atcgcataag ggtacaccct gatgcgagat ataaaatgtg acctcataca
    11821 attaatgaca taacctatat gcgatagaat atatgcaaca acctaaatgc gataatgcgg
    11881 tgaaaaaatg ttcatcagag cttatttaag ggcgtcaaca gaagatcagt ttgcagatcg
    11941 ggcaaaagag atgctggaac agttcgtcca ggaacggggg cataaaattg ccagttatta
    12001 tcgggagaac ataagcggta caaaacttga ccgtccggaa ctggggcgat tactgatgga
    12061 cagtcaccgt aatgatattt tactggttga gcagatagac cgcctgaccc gtcttagcaa
    12121 cagcgactgg ataacgctga aaaagcaaat tgaacaacat gaattgcgaa ttgtcagtct
    12181 ggatgttcca acatcatggc aagccctttc agataaagat ccttcacaat cagatccgat
    12241 tacccgcgca gtgataactg caataaataa tatgcttata gacctgatgg ccgcgatgtc
    12301 gcataaggac tggctgagtc gtcgccaacg ccaaaaacag ggaattgaac gagctcatac
    12361 gttgggtaaa taccggggaa aacaagccga tcaggaacgg catcagaaag tgttgtacta
    12421 cagacaagta aaaaaactca gcattagaga aacagcagat gctacgggct acagcacttc
    12481 gcaggtttgc cgtattcagg cactttatcg agaaccaacg actgattaaa aagtagcctt
    12541 aacgtacaat aattttcgat atccaaactg acccc
//
