LOCUS       NZ_CP124458             4087 bp    DNA     circular CON 10-JUN-2024
DEFINITION  Escherichia coli strain AVS0193 plasmid pAVS0193-C, complete
            sequence.
ACCESSION   NZ_CP124458
VERSION     NZ_CP124458.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34360967
            Assembly: GCF_030037975.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Biggel,M.
  TITLE     Genomic characteristics of ESBL-producing Escherichia coli ST131
            isolated from the water ecosystem in a defined region in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030037975.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:19:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,282
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,921
            CDSs (with protein)               :: 4,921
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 249
            Pseudo Genes (ambiguous residues) :: 0 of 249
            Pseudo Genes (frameshifted)       :: 88 of 249
            Pseudo Genes (incomplete)         :: 188 of 249
            Pseudo Genes (internal stop)      :: 40 of 249
            Pseudo Genes (multiple problems)  :: 57 of 249
            Pseudo Genes (short protein)      :: 1 of 249
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124458.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AVS0193"
                     /isolation_source="environmental: river near WWTP effluent"
                     /db_xref="taxon:562"
                     /plasmid="pAVS0193-C"
                     /geo_loc_name="Switzerland: Kanton Zug"
                     /collection_date="2020-11-26"
                     /collected_by="Amt fuer Verbraucherschutz, Kanton Zug"
     gene            complement(join(4086..4087,1..706))
                     /locus_tag="QJP76_RS26330"
                     /old_locus_tag="QJP76_26330"
     CDS             complement(join(4086..4087,1..706))
                     /locus_tag="QJP76_RS26330"
                     /old_locus_tag="QJP76_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1238..1492)
                     /gene="mobC"
                     /locus_tag="QJP76_RS26335"
                     /old_locus_tag="QJP76_26335"
     CDS             complement(1238..1492)
                     /gene="mobC"
                     /locus_tag="QJP76_RS26335"
                     /old_locus_tag="QJP76_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1671..3830
                     /gene="mobQ"
                     /locus_tag="QJP76_RS26340"
                     /old_locus_tag="QJP76_26340"
     CDS             1671..3830
                     /gene="mobQ"
                     /locus_tag="QJP76_RS26340"
                     /old_locus_tag="QJP76_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023142279.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTEYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLATL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(1494..1670)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1671..2029
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..706)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1585..3827
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.599"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agatcgtcat cttgatctgt ttgtcttcct tgaagcgaaa atgcagggct acaaccgtgc
       61 gccccttttt gatcgtctca accgttacag ccaggtcgct ttttttgttc agttcgtcaa
      121 cacagggttt tattagatcc ctgtttaacg ttttgaaatc tttatattta ccatcaagca
      181 tcagtgatga tttaaaatca tcgagggcga taatacgttc cccagtactg cggaattgct
      241 ggagaatttc gtatattctt atgctgtaac ttcttgataa attagaaata tttttaatga
      301 cgactctggt gaattgcccc ttcaactgtg tcagataagg cattaccgcg tcagaaaaag
      361 tgatttgtgc tttcccctct cccctcgcgt actgtgcccg atactgaatc cagcggaact
      421 cctcgcgctt ctcgtcgtcc ttgagaatga tcgacctgtc ccacaaacga tcgatcgctt
      481 cctgtagctg aacctcggca tttttccgcc ccatatcagg gaatgaatca aaatattccg
      541 cagctgtaat ggtcattgtc ttttgcaatt caggtgaagc tacgtcagta ccggagttaa
      601 gtcggcttat gcagagcaat aagaaacgct gttcttgtaa agttagcttg taactggcct
      661 caaccagcgc attggactta taggctatca gagggtttga tgtcatttta accaccaggc
      721 caaaaatgag gtgaaataaa aacctatcac ctttagcatt ggtgagcaat tatattttca
      781 ccccccattt tcctgtgaat attaccttaa atctcctcac ctttacctta aattacctca
      841 cccaagcacc ttaaattata tcacctttac cttaaatcat atcacctata gcgttgtaac
      901 attttgtttt ctataaggaa ttttggcgct aaaacaagta ttaaaaacaa gatcttaaaa
      961 acaagatcta aaaacttaac gacgagcctg tggataactt ggagtgctga aaacaaaaaa
     1021 ccaataaaaa caatgcgttg caaaaaagca gaattgcaag ttccgaatac accccctcca
     1081 accctgtgga aatccagtat tcatgcgggt tcagcgcata tcacctcccg aaaaaccgtg
     1141 cgtatgtgct caatcaggcg acggattcac ttccgcttcc tcgcccactc gctcgctaac
     1201 gctcggtcgc tgggactgcg gcgagcggac gatacaatca ctgtcctggc agcgcttcca
     1261 gctcgttaat cagcggcagt atgcgcgtca catcagcctg tcgtgtcacc tctgccttca
     1321 ggaactggag taaccaggca cgcaactgca cctcctccgg ctttctgacc tttgccagca
     1381 atgctgcacc cacaatgacc ttctgcgctg tgtccagact cctgctcttc gccttcaggc
     1441 gctgtaatct ggcctcagca tcagcaatct tctgttcgag tgttctgctc atgatgtaac
     1501 tccgtgcgcg atgaaaaatc gcattttagc gcgtcactgg tagtttaaaa actgagctgg
     1561 cataatgcac ggaaaatcac gaagtgcgca cttatacaat ctccactccg tttcgattgt
     1621 gtgcgccctg cggggctgaa agaaaaccac aaaaaggcat tacggcagaa atggcgattt
     1681 atcatctcag catgaaaatc atctccagaa acagcggcta cagcgccgtt gcttctgctg
     1741 cctatcgttc cggttcactg atgcttgatg agcgtaccgg actgactcac gactacaccc
     1801 gcaaaagcgg tgtggctgaa gctgtgattc tgactcccgc gacagcccct gcctggtgta
     1861 cgaaccgtgc cgaactctgg aatgccgttg agaaggccga acgccggaag aactcacaac
     1921 ttgcgcggga aattgaactc gccatccccc gcgaactacc gcaggatgcc gcacgggaaa
     1981 ccgttctcgc ctttgtcaga gaaaactttg tcagtcaggg catgattgct gatgtggcgt
     2041 tccatcacat ggataaaaca aatccccatg cgcacatcat gctgaccacg agagctgtcg
     2101 gacctgctgg attcggtgga aaggtcaggg actggaacga ccggacacat gccgagacgt
     2161 ggcgtgcatc atgggctgac catgcgaaca gggcactggc gaacgccggt tatcaggaag
     2221 agatagacca tcgttcatac gagcgacagg ggcttgagaa aacgcctggt attcacctcg
     2281 gaaagtctgc ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc gaacagaatc
     2341 gtctgattaa cagacttaac ctggaaatac agatttcccg taccgaactc agaaacagcg
     2401 ggctgacaga gcctgcccgc cgtgtggctg accttctgaa tatcgtgctt cctgacaacg
     2461 caacttcgta cacactacgt gacatcatcg aagcgttgcc gaaggacagt aatgcggcat
     2521 ggaaactcac cagtaatgcc atgtcgatga tggcagatat gcaggcgacg cgcagacgct
     2581 gggaagagct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc ctgaaaaaat
     2641 catcgccgtt ttcgtccggt ttttcccgta ttcccctgat gcaatgggcg gcaccggaat
     2701 accgtagcga acagggaaaa atccagtcgc tgagccagca aatggaaaaa ttgcgacagc
     2761 attaccggaa ggtggagaaa caggacattc ccgcgagtca gacggcattt gaaaaacagt
     2821 ggcagcagtg gggggcatcc ggccttgcag ctctcagagt ccagttgctt aaacgtgaag
     2881 aggaattgcg ccggaaggag caggaggata cagaacgtag tcgcgcggag cgacgagcgg
     2941 aacagctcag gaaaaatgac aatgccgtta tggattcagg ggtactgagc gcagtcgtta
     3001 cggagtatgg cgaggccccc atgcctggta acggggaaat gacctgttac cttctgcttc
     3061 acaaccggaa cggagaatac acgctctggg ggaacgagct ggagaaatac cgcaccagaa
     3121 tctttgagcg tgttgattta atgcgtgaca ggagtggtta tatctgtgac cgaagcgaaa
     3181 tcggtcaaca tccaccactg caacgtgttt attcctctgc gacctttgag caacttctga
     3241 cgcaggtttg ccagacatgg ccgcaatata cccgcaatct ccgccagccg aagacatggc
     3301 cggagtcttt ctgtcttgga gaagaccgcc agccagccat gccatcactg gcagcaagga
     3361 aggtggattt tacgcaggga cgtctgctag cgacgctgat gccggtaatg agttctgtcg
     3421 accgggaaac ccgccagctt cagcttttgc tggtcatggg cgtggatgac tctctgggcg
     3481 gtgttgtccg gcttaatggt acgctgtatc ccgcgtttgc cgttccctca gccgataaca
     3541 gccagctggt catcagtgca ctgaccgata aggggttacg gtatgcaggg tatggtgttg
     3601 ccgtaaatca cgatgcagac agccacatat cacctgcgcc ggaacttatg gagtttcatc
     3661 tgaaaacccg tgaggcaccg ctttttgctg ccgtcaacac accagaaaaa cagccggatc
     3721 atctgttccg ctcgctgggt tttaatcgta cctgggacga atggcggcgc gaggaagatg
     3781 cacgaacaca cgccaccgag aggagacatg atcgcggttg gtcacagtga atgtgaacaa
     3841 tattttgttt tgtttaacat cgtgttatgt tcgtcgatgt tcttcgatgt tcatcgacag
     3901 ggatgaatgc aagaagcaca cacataatgc aagcatagtt ttgtttttcc gcccccgaaa
     3961 aatgggggtt cttttttcgc tgcattagag cgcttttgac gcaatttttc acttcaatag
     4021 taccttcgca ctactaacac aaaaaaaacg cagcagggag cgttttaggt gccttaaaat
     4081 cgattct
//
