LOCUS       NZ_CP124432             6647 bp    DNA     circular CON 10-JUN-2024
DEFINITION  Escherichia coli strain AVS0051 plasmid pAVS0051-C, complete
            sequence.
ACCESSION   NZ_CP124432
VERSION     NZ_CP124432.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34360971
            Assembly: GCF_030038915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Biggel,M.
  TITLE     Genomic characteristics of ESBL-producing Escherichia coli ST131
            isolated from the water ecosystem in a defined region in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030038915.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:14:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,006
            Genes (coding)                    :: 4,785
            CDSs (with protein)               :: 4,785
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 88 of 221
            Pseudo Genes (incomplete)         :: 158 of 221
            Pseudo Genes (internal stop)      :: 43 of 221
            Pseudo Genes (multiple problems)  :: 58 of 221
            Pseudo Genes (short protein)      :: 1 of 221
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124432.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AVS0051"
                     /isolation_source="environmental: river/lake"
                     /db_xref="taxon:562"
                     /plasmid="pAVS0051-C"
                     /geo_loc_name="Switzerland: Kanton Zug"
                     /collection_date="2020-08-05"
                     /collected_by="Amt fuer Verbraucherschutz, Kanton Zug"
     gene            complement(join(6644..6647,1..188))
                     /locus_tag="QJP74_RS25425"
                     /old_locus_tag="QJP74_25425"
     CDS             complement(join(6644..6647,1..188))
                     /locus_tag="QJP74_RS25425"
                     /old_locus_tag="QJP74_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            252..575
                     /locus_tag="QJP74_RS25430"
                     /old_locus_tag="QJP74_25430"
     CDS             252..575
                     /locus_tag="QJP74_RS25430"
                     /old_locus_tag="QJP74_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            784..>1215
                     /locus_tag="QJP74_RS25435"
                     /old_locus_tag="QJP74_25435"
                     /pseudo=""
     CDS             784..>1215
                     /locus_tag="QJP74_RS25435"
                     /old_locus_tag="QJP74_25435"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1253..1771
                     /locus_tag="QJP74_RS25440"
                     /old_locus_tag="QJP74_25440"
     CDS             1253..1771
                     /locus_tag="QJP74_RS25440"
                     /old_locus_tag="QJP74_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            1778..2011
                     /locus_tag="QJP74_RS25445"
                     /old_locus_tag="QJP74_25445"
     CDS             1778..2011
                     /locus_tag="QJP74_RS25445"
                     /old_locus_tag="QJP74_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2329..2748)
                     /locus_tag="QJP74_RS25450"
                     /old_locus_tag="QJP74_25450"
     CDS             complement(2329..2748)
                     /locus_tag="QJP74_RS25450"
                     /old_locus_tag="QJP74_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2829..3266)
                     /locus_tag="QJP74_RS25455"
                     /old_locus_tag="QJP74_25455"
     CDS             complement(2829..3266)
                     /locus_tag="QJP74_RS25455"
                     /old_locus_tag="QJP74_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3526..5094
                     /gene="cea"
                     /locus_tag="QJP74_RS25460"
                     /old_locus_tag="QJP74_25460"
     CDS             3526..5094
                     /gene="cea"
                     /locus_tag="QJP74_RS25460"
                     /old_locus_tag="QJP74_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(5091..5432)
                     /locus_tag="QJP74_RS25465"
                     /old_locus_tag="QJP74_25465"
     CDS             complement(5091..5432)
                     /locus_tag="QJP74_RS25465"
                     /old_locus_tag="QJP74_25465"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058759.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            5480..5617
                     /locus_tag="QJP74_RS25470"
                     /old_locus_tag="QJP74_25470"
     CDS             5480..5617
                     /locus_tag="QJP74_RS25470"
                     /old_locus_tag="QJP74_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(5816..5974)
                     /locus_tag="QJP74_RS25660"
     CDS             complement(5816..5974)
                     /locus_tag="QJP74_RS25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     Typing          6472..6559
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(5903..6458)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          566..2115
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggttttcac cgtcatcccc gaaacgtgcg aggcagctgc ggtaaagctc atcggcgtgg
       61 tcgtgaagcg attcacaaat atcggcctgt tcatctgcgt ccagttcgtt gagcttctcc
      121 agcagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
      181 ttagtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg ataccgatga
      241 aacgcgagag gatgctcaca atacgggtta ctgatgatga acatgcccgg ttacttgaac
      301 gctgtgaggg taaacaactg gcggtatgga tgcggcgggt ctgcctgggg gagccggttg
      361 cccgttccgg aaaactgccg acactggcac cgccgttact gcgtcagctg gccgccatcg
      421 gaaataacct gaatcagaca gcccgtaagg tgaacagcgg gcagtggtct tccggtgacc
      481 gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg
      541 ccgtcaggga acagggggcg cgggatgata gttaaatttc atgccagggg aaaaggtggt
      601 ggcagtggtc cggttgatta cctgctgggg agggagcgta accgcgaagg cgcaacggtg
      661 cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa
      721 tacacgtccg gtgttctgtc gttcgcggag aaggagctgc cgccgggagg acgtgaaaaa
      781 gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatc
      841 ctgtgggtgg agcaccagga caagggacgg cttgagctga attttgtcat tccgaacatg
      901 gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag gcctagaatt
      961 gatgcctggc agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag
     1021 aaccgcagga cgctgacact ccctgataac ctgcctgaaa cgaaacaggc gcttgctgag
     1081 ggcgtcacgc gaggtataga tgcactttac catgccggag agataaaagg ccgtcaggat
     1141 gtgattcagg cgctcactga ggcggggctg gaagtggtca gggtgacgcg aagcagtatc
     1201 agcattgcag atccgaacgg cgggaagaat atcaggctga aaggagcatt ttatgagcaa
     1261 tcttttgcag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga
     1321 gaaaatgctg aacaacgagt tcaggaggct cggcgaatct gtaagcgagg ctgtgacatc
     1381 aaacgagacg aaaatcagag acgctatagc cctgttcaca gcctcgacag aggaatcgct
     1441 ggaaaaacac cgggaagggg tgaaagaggc gatgatgcag cacaggaggg acgtgttaaa
     1501 gctggcaggg aatacgggca tgatgttact ggggatagtc tttctcctgt ttaccgcgag
     1561 tggcgggacg ctctggtatc ttggagggag gatacaggcg aacctggaag aaatcaggaa
     1621 gcaggaagag acattgcaga aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga
     1681 cgggaacagg aaattccttg tccttccgta cgggaaatca gcggaggtga ttccctttca
     1741 ggggaaagag tgggtacatc tgaaggagtg acacagagtg acagagctgg aaacacattt
     1801 gctgaacgcc ttagagcagc tgcaacagga ctatatgcag cggctgagcg aatgggagag
     1861 cgccttcgtg gaattgcaga agatgttttc gcttacgcaa cgggacaacg cgatgctgaa
     1921 cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga cagaacgctt
     1981 gagccagtta tacagcgaga actggagata agagaggaac ggctgataca ggagcgcgaa
     2041 catgtgttat ccctggaacg ggagcgtcag ccggaaatac aggaacgcac gctggatggc
     2101 ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc agtggattaa gtgggggtaa
     2161 tgtggcctgt accctctggt tgcataggta ttcatacggt taaaatttat caggcgcgat
     2221 cgcggcagtt tttcgggtgg tttgttgcca tttttacctg tctgctgccg tgatcgcgct
     2281 gaacgcgttt tagcagtgcg tacaattaag ggattatggt aaatccactt actgtctgcc
     2341 ctcgtagcca tcgagataaa ccgcagaaat cgtgtcagcc agcagcctgg attttttcgg
     2401 ggtaaggtct ttgctgcggt aatcagtacc cgcaaattga gttgtgcccc ggagtgagtt
     2461 taaaaattcc tggctgtgaa actgctgaat gcgttgttgc acatcactcc gtgattttcc
     2521 tgtcgcttta tctttagctc ccatatccca gaactgccga aaaaaaggca ccatcattcg
     2581 tgttgtgtcg gctttttggg tggaaaagtt gggatcaaaa ccgtcatcag aagcatatac
     2641 gtaatgccgg gtatgttgtt ccacgctgcc ctttggggta caggctgtga gagctactgt
     2701 tgctgttatc atggcgataa gacaaagttt gtttccggta cgtctcatga gtgtctccct
     2761 accttaaagt atttattctc ggtgagttat tatccgaaga ctattttatc atcagtctct
     2821 gacagcgttc agcgaccgaa caacctggac cagaatcccc gtttcttttc cagttccagt
     2881 gcctgcctga gctgggctat ctcattttgt agctgttccc gttctgcttc ctggcgtctg
     2941 cgatccatat cctgtgcctg tttatcctca agcatcagcg tcaggcattg tttcagctca
     3001 ttcagctccc ggagaatgcg ttctgtctgc tgatcatgtg gattttctgc atgtccctca
     3061 ctgtgccttt ctggtgtctc attctgcttt aattcgccgt atgcccggat cagttcactg
     3121 gtttcaaact cccgtcgtcc gtccctgccg gtacggtaac tgacaaggcc tgcgcgcata
     3181 tcacggtaaa actgtgaacg cgatctgcct gtcattttta gtgcgtcccg gagtgtgtgc
     3241 catgccataa agtgacagtg tcccatagat gtctcatctc atagtttcag taaaacataa
     3301 tgaggtctga gaacggtaat gtttgtgctg gtttttgtgg catcgggcga gaatagcgcg
     3361 tggtgtgaaa gactgttttt ttgatcgttt tcacaaaaat ggaagtccac agtcttgaca
     3421 gggaaaatgc agcggcgtag cttttatgct gtatataaaa ccagtggtta tatgtacagt
     3481 atttattttt aacttattgt tttaaaagtc aaagaggatt ttataatgga aaccgcggta
     3541 gcgtactata aagatggtgt tccttatgat gataagggac aggtaattat tactcttttg
     3601 aatggtactc ctgacgggag tggctctggc ggcggaggtg gaaaaggagg cagtaaaagt
     3661 gaaagttctg cagctattca tgcaactgct aaatggtcta ctgctcaatt aaagaaaaca
     3721 caggcagagc aggctgcccg ggcaaaagct gcagcggaag cacaggcgaa agcaaaggca
     3781 aacagggatg cgctgactca gcgcctgaag gatatcgtga atgaggctct tcgtcacaat
     3841 gcctcacgta cgccttcagc aacagagctt gctcatgcta ataatgcagc tatgcaggcg
     3901 gaagcagagc gtttgcgcct tgcgaaagca gaagaaaaag cccgtaaaga agcggaagca
     3961 gcagaaaagg cttttcagga agcagaacaa cgacgtaaag agattgaacg ggagaaggct
     4021 gaaacagaac gccagttgaa actggctgaa gctgaagaga aacgactggc tgcattgagt
     4081 gaagaagcta aagctgttga gatcgcccaa aaaaaacttt ctgctgcaca atctgaagtg
     4141 gtgaaaatgg atggagagat taagactctc aattctcgtt taagctccag tatccatgcc
     4201 cgtgatgcag aaatgaaaac gctcgctgga aaacgaaatg aactggctca ggcatccgct
     4261 aaatataaag aactggatga gctggtcaaa aaactatcac caagagccaa tgatccgctt
     4321 cagaaccgtc ctttttttga agcaaccaga cgacgggttg gggccggtaa gattagagaa
     4381 gaaaaacaaa aacaggtaac agcatcagaa acacgtatta accggataaa tgctgatata
     4441 actcagatcc agaaggctat ttctcaggtc agtaataatc gtaatgccgg tatcgctcgt
     4501 gttcatgaag ctgaagaaaa tttgaaaaaa gcacagaata atctccttaa ttcacagatt
     4561 aaggatgctg ttgatgcaac agttagcttt tatcaaacgc tgactgaaaa atatggtgaa
     4621 aaatattcga aaatggcaca ggaacttgct gataagtcta aaggtaagaa aatcggcaat
     4681 gtgaatgaag ctctcgctgc ttttgaaaaa tacaaggatg ttttaaataa gaaattcagc
     4741 aaagccgatc gtgatgctat ttttaatgcg ttggcatcgg tgaagtatga tgactgggct
     4801 aaacatttag atcagtttgc caagtacttg aagattacgg ggcatgtttc ttttggatat
     4861 gatgtggtat ctgatatcct aaaaattaag gatacaggtg actggaagcc actatttctt
     4921 acattagaga agaaagctgc agatgcaggg gtgagttatg ttgttgcttt actttttagc
     4981 ttgcttgctg gaactacatt aggtatttgg ggtattgcta ttgttacagg aattctatgc
     5041 tcctatattg ataagaataa acttaatact ataaatgagg tgttagggat ttaattattc
     5101 tttattgata taaaaagtcc tagcaatcca aatgggattg ctaggaccaa acaaagtaga
     5161 ttatatagca taaataggtt taattttgct acgggggcgt tatttaggtt ttttcttctt
     5221 tcgaaaaaat ctttctttat gaagttaaaa gctatgtgtt caatagcata ttttgaatat
     5281 ggacatagaa tagtgcttat cactattgca tatagcatct tatctgacac aaggaaataa
     5341 tacccttcgc tgttttttgt tataaggtat atatatataa gtgtgcagta caggccaaat
     5401 aaaatatttt ttatgtagta tcttaagctc ataaattaaa cctcgccata tattcttttc
     5461 attttataag gatcgagtta tgaggaaaag attttttgtg ggaatattcg cgataaacct
     5521 ccttgttgga tgtcaggcta actatatacg tgatgttcag ggagggacca tcgcaccatc
     5581 ctcctcttct aaactgacgg ggatcgcggt tcagtagaaa agatcaaagg atcttcttga
     5641 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccaacg
     5701 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5761 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agtcgggcca ctacttcaag
     5821 aactctgtag caccgtttgt gccatcatcg ctctgctaat ccggttacca gtggctgctg
     5881 ccagtggcgt taaggcgtgc cttaccgggt tggactcaag acgatagtta ccggataagg
     5941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6001 acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gcctgctacg
     6241 tggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcctt atcccctgat
     6301 tctgtggata actgtgttac cgtttttgtg tgagtcagta ccgctcgccg cagtcgaacg
     6361 accgagcgta gcgagtcagt gagcgaggaa gcggaaaagc gcctggacgt gcattttctc
     6421 cttacgcatc tgtgcggcat ttcacacccg gcatggcgta cttttcatac aatccgcact
     6481 gatgccgcat ggttaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctg
     6541 cgccccgaca cccgctaaaa cctgctgacg cgccctgacg ggcttgtcag ctcccggcat
     6601 ccgctcacag acaagctgtg accgtctccg ggagctgcat gtgtcag
//
