LOCUS       NZ_CP124404             2113 bp    DNA     circular CON 10-JUN-2024
DEFINITION  Escherichia coli strain AVS0528 plasmid pAVS0528-D, complete
            sequence.
ACCESSION   NZ_CP124404
VERSION     NZ_CP124404.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34360978
            Assembly: GCF_030039425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Biggel,M.
  TITLE     Genomic characteristics of ESBL-producing Escherichia coli ST131
            isolated from the water ecosystem in a defined region in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030039425.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:21:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,974
            CDSs (total)                      :: 4,865
            Genes (coding)                    :: 4,655
            CDSs (with protein)               :: 4,655
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 83 of 210
            Pseudo Genes (incomplete)         :: 151 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 53 of 210
            Pseudo Genes (short protein)      :: 1 of 210
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124404.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AVS0528"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pAVS0528-D"
                     /geo_loc_name="Switzerland: Kanton Zug"
                     /collection_date="2021-06-17"
                     /collected_by="Amt fuer Verbraucherschutz, Kanton Zug"
     gene            372..1337
                     /locus_tag="QJP80_RS24830"
                     /old_locus_tag="QJP80_24830"
     CDS             372..1337
                     /locus_tag="QJP80_RS24830"
                     /old_locus_tag="QJP80_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1341..1607
                     /locus_tag="QJP80_RS24835"
                     /old_locus_tag="QJP80_24835"
     CDS             1341..1607
                     /locus_tag="QJP80_RS24835"
                     /old_locus_tag="QJP80_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          766..997
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gatcgggtgt gtggaaaagg ttggggtaag ccgttcgggg gtgcttgttt tggggggtta
       61 aaattgtggt tattttttgc gcaattctcg cgcgtgatcc ttgtatttat acttaaggga
      121 taaatggcgg atatgaaata gtggtttagc ccagtaatga cgaggctttg agtgggtttt
      181 gacaggtcaa agaaaatgga gcagaattga ggcgttttta atcggcgttg gggagtgcgt
      241 caacactccc caacatttcg aatgtgtcac ctcagcggca aactctggtg acatgtactg
      301 gctcgcaatg cacaggtacg tgatgaatat accacatcaa atcacagcct gcccagatcg
      361 gagcaggctt aatgtcagaa gataaattcc tttcggacta cagcccccgt gatgcagttt
      421 gggataccca gcgcacgctt accgattctg tcgggggtat ctaccagact gctgctgaat
      481 tcgagcgcta tgcactccgt atggcctcct gtagcggttt gttacgtttt ggttggtcta
      541 ccatcatgga aaccggagaa acgcgcctac ggcttcgtag tgcgcaattt tgccgtgtcc
      601 gtcattgccc tgtctgccag tggagaagaa ccctcatgtg gcaagcccgt ttttatcagg
      661 ctctaccgaa aatcgttgtg gattacccgt cttcccgatg gttgtttctg acgttaactg
      721 tcaggaactg cgagataggt gaacttggaa cagtccttac agcaatgaat gcggcgttta
      781 agcgaatgga aaagcgaaag gagctatcac ctgttcaggg gtggatcagg gctacggagg
      841 tgacgcgagg taaggatggc agcgcacatc cgcattttca ctgtctgctg atggtgcaac
      901 cttcttggtt taaagggaag aactacgtta agcacgaacg ttgggtagaa ctctggcgcg
      961 attgcttgcg ggtgaactat gagccgaata tcgatattcg ggcagtaaaa actaagacag
     1021 gtgaggttgt ggccaacgtt gccgagcaac tgcaaagcgc ggttgctgaa acgctgaaat
     1081 actccgttaa accggaagat atggcaaacg atcctgagtg gtttcttgag ctgacgcggc
     1141 agcttcacaa gcgccgtttt atctcgaccg gtggggcgct aaaaaacgtc ctccagttgg
     1201 atcgagaaac caatgaggat cttgtcattg ccgacgatgt aggggatggc actgatgacg
     1261 ggaagcggac ggcgtttgtc tgggattcag gtaaacggcg ttacaaacgc gcccctgaga
     1321 aggataaatc ggattaacgt atgaatatta atattgaata cctgaatgga aataagacta
     1381 ttggtttatt ttttttaaga agtgaagcgg tgattcctga caggtttaaa aaccttattt
     1441 tgcttattga tggattaagt tttggcacat ttggttttca tccgcacgaa ggttttgagg
     1501 atgaattaat tttatatatt cagaaaacaa acgagagggt aaaaactctt tttgtgaaaa
     1561 tagatttaaa cggtatcaag tttggatttt taagaacgca ttcttagttc tggaaaagag
     1621 ccagcggcag gctgaggtga taggtacgag attgcatgca atctctagtg ctctgtctat
     1681 cctgcattat cctcagcatt atcctcagca ttatcctcag cattatcctc agccttgcca
     1741 actcgacacc aatgcaggat agacaatccg atgtcaaatg ttaacactct gcgagtggta
     1801 cattttcccc ggattatcgt cctgagcctg ccgctggctc tctttctacc gcctcgcttt
     1861 gctcgttgct caacgcctca cagacacgga ttaaaatccg catccgttca ccgtttttta
     1921 aagtccgtta aaagcatgat gccatctccg agagttaatc tcgtcaaatg ctaaatcgtg
     1981 ggggtcccct ttggggttcc gatttagtga ttgacgacac caccgattaa aaaacttatg
     2041 cggggtggat ggtttcacga agtgaggcca tccacctgta agacagggtt ttgtttttat
     2101 tccctgtttt ggt
//
