LOCUS       NZ_CP124403             5072 bp    DNA     linear   CON 10-JUN-2024
DEFINITION  Escherichia coli strain AVS0528 plasmid unnamed.
ACCESSION   NZ_CP124403
VERSION     NZ_CP124403.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34360978
            Assembly: GCF_030039425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Biggel,M.
  TITLE     Genomic characteristics of ESBL-producing Escherichia coli ST131
            isolated from the water ecosystem in a defined region in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030039425.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:21:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,974
            CDSs (total)                      :: 4,865
            Genes (coding)                    :: 4,655
            CDSs (with protein)               :: 4,655
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 83 of 210
            Pseudo Genes (incomplete)         :: 151 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 53 of 210
            Pseudo Genes (short protein)      :: 1 of 210
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124403.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AVS0528"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="unnamed"
                     /geo_loc_name="Switzerland: Kanton Zug"
                     /collection_date="2021-06-17"
                     /collected_by="Amt fuer Verbraucherschutz, Kanton Zug"
     gene            complement(358..756)
                     /locus_tag="QJP80_RS24800"
                     /old_locus_tag="QJP80_24800"
     CDS             complement(358..756)
                     /locus_tag="QJP80_RS24800"
                     /old_locus_tag="QJP80_24800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            933..1859
                     /locus_tag="QJP80_RS24805"
                     /old_locus_tag="QJP80_24805"
     CDS             933..1859
                     /locus_tag="QJP80_RS24805"
                     /old_locus_tag="QJP80_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1862..2395
                     /locus_tag="QJP80_RS24810"
                     /old_locus_tag="QJP80_24810"
     CDS             1862..2395
                     /locus_tag="QJP80_RS24810"
                     /old_locus_tag="QJP80_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2461..2670)
                     /locus_tag="QJP80_RS24815"
                     /old_locus_tag="QJP80_24815"
     CDS             complement(2461..2670)
                     /locus_tag="QJP80_RS24815"
                     /old_locus_tag="QJP80_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(3819..4331)
                     /locus_tag="QJP80_RS24820"
                     /old_locus_tag="QJP80_24820"
     CDS             complement(3819..4331)
                     /locus_tag="QJP80_RS24820"
                     /old_locus_tag="QJP80_24820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_097515206.1"
                     /translation="MISGAFVFQSLTLHPTANPVEPSRKPDPFTITPVVRPLKTPSVAF
                     WRKKRKKTRKHQAKPEKRVRIVFRALHNYARKRCAIGFVRSEHRTAQNDKFSPLVRGAL
                     TGFPVSKLHISPGQKKTDLGSVARIRVYGKYFSYRMLNIHKRKKCLSDALKQPFQKESA
                     LYDARKR"
                     /name="hypothetical protein"
     gene            4406..>5072
                     /locus_tag="QJP80_RS24825"
                     /old_locus_tag="QJP80_24825"
     CDS             4406..>5072
                     /locus_tag="QJP80_RS24825"
                     /old_locus_tag="QJP80_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_283192277.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     "
                     /name="replication initiation protein"
     Typing          4917..5069
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.805"
                     /coverage="100.0"
                     /evalue="2.27e-61"
     Typing          934..1856
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccagaaatt caggctgcac gcggtcgcaa gggcggttct aagtctaagc gcagcacagt
       61 agctacatct gcacgcacgc tgaaaccgtg ggaggcatta gggatcagtc gcgcctggta
      121 ttaccaactg aaaaaacgag gtctcgtaga gtagaccaaa taagcctata tcagataaca
      181 gcgcctttct ggcgtctttt tgagcagcag gttttttgcc gtttttcccc gttcagccct
      241 gcaatggcgc ggctttccgc gtgattgtgg tggtagtgct cgccgcagtc tcatgaccga
      301 gcgtagcgag cgaatgagcg aggaagcacc agggaatcgg aacacggatg gaggcactta
      361 cgcgtctggg tttggggatg tctccagatc gtacttaagt tccttaagtg cttgtaatat
      421 cctgtctttt ttatagcata gctcgcctgt ttttgtgtct accagtcctg cttcgatcag
      481 tagcccagcc gcgagaaaga gccggtgatt gcgttcccgt cgttgttttt ttccctcgat
      541 tcgtttttgg accgcctgtt ttcgttcgct ctcctgtgcc tgtaagtcgt ttttttccgc
      601 tgttgccagt acgtggagaa gtttgcgttc ctgttctgtg agtccggctt cgttgtcgat
      661 caggtcaata atctgtctct gtcgtgcggt ctgttctccg agcttcctga tgaatccgat
      721 ccgtttcttc agccattcga tgttgctgat gcgcatgttc gtgatgctcc ctggtgtttt
      781 tttgcggtgt gttgtggtcg tgcagtatat cgcttgcggt cggcgggtgc agggggtatc
      841 ttccgtgcta attcgtaaaa ttagaaggaa gcgcacttat atgtttctct ccttcgtcga
      901 gaaacagtgc gctcctgcag agggctgaaa ccgtggcaac ctatcacctc agcgttaaat
      961 ttggtggcaa ggggcaggct gcaaaccacg ccgattacat cgagcgcaaa gagaaatacc
     1021 gcgaccgtca ggatttggaa tattccgcac acggaaacat gccggaatgg gccagggata
     1081 atccttccca tttctggcag gcggctgacc agtttgaacg tgcgaacggt tcgacctata
     1141 gggaacttga aattgcatta ccccgtgaac tgacaccgga acagcgcctt gaactggtgc
     1201 aggattttgt acgtcaggaa gctggcgaac ggcacgcctg gagttttgcc attcacaatc
     1261 cgaaagcgag cattgacggc ggcgaacagc ctcatgcgca tatcatgatg agtcagcgtg
     1321 tgaatgatgg cattgaacgt acaccggaac agtatttccg gcgctataac gccaggtatc
     1381 ccgagcgggg aggtgcgaaa aaggattccg gaagcctgac tctcactcag caaaaagagc
     1441 agttgcggga attgcgaaaa cgctgggagg tgaagcacaa cgaacatatg agaaagcatg
     1501 gctttgaaag gggcttcatt gactgccgga cgctgaaaga acagggcatt gaacgacgtc
     1561 ccgaagtgca tctgggtttt gaagctgccg gacggcttga tgatgcccag cgtcatgaca
     1621 tcatgcaaaa acggtctgaa ccggactatt cccgccatct tgaacaggcg cgaacggtcg
     1681 ggcatgacat cagggaatgg ctggacactc cggttaagca acatgaactg gtcagaaggc
     1741 tgcaacatga tgttgcgcgg gagcatggct ttgacatgag agacagagtg agcagacaga
     1801 accgaactgc acctgaacgg gttaaatcac cgtcacgtgg attttctatg ggattctgaa
     1861 catggccagc acagaagaaa aattacttac gctcctgtta tcggtggagc atctgcaaca
     1921 taccgcaatg gagcaacaga aagctgttgc tgattctctg gctgtggcaa gggaaactct
     1981 tcgcggatat cgggatattt ctgacggtgt ggcaaaacag gtcagggatg aagtcagaaa
     2041 tgaggtgcag aagctggacg tggcaggact gataggtgag cgcatcagcc aggattttca
     2101 gcggctggaa gagtcagtcg caaacatgaa ggcaaacgcg gatgccctga acagggatat
     2161 tgcgacaatt cacagcaata tcgtgcagga atacaacacc cttaaagggt atagctggaa
     2221 atggctgggt ggggtgtcag tggcttttct ggttatgttc tgtgtgctcg gatggggaat
     2281 gaaaacaata ctcgacagga actacaacgc cacagcggcg gtgtatcaga aagttgtgac
     2341 actggaagat aaactggtgg agaaacctgt cacggggaag agccggaaaa actgacgcgc
     2401 ttcgcttgtc caacgcctca cgacccgcga aagaatttcg cgaaccgttc accgtttttt
     2461 tcaatatgat tctgtctgct ttggtcgata aagcagacga tagtattcca gccttcgtat
     2521 atataacggt acgtgctcat cagtaagagc tggcggtatt gtgttatcag ggggaagttt
     2581 tttcagatac tcacgaaatg cctggcttga tgccataaaa tctacagtag catcgataat
     2641 atgtggggtg ttttcaccta attttgccat gatgataatc cttatattat cacggtttgt
     2701 gaatgatagg gtgtcactat tatttatgcg ttagtgaacc agaaaaaatc ccccgttggc
     2761 atagggggat atgtaaggag aaataacttc gcaggtgttc ttgtttgtat aacgtgaaaa
     2821 gactgcacgc aacttaataa gtctgccgta cttactttca gttttcaaca taaggtttat
     2881 aacaatgtat ggaatggaag tgagctttaa ggtttcagct ctaaaaaata aaatatttat
     2941 tagttggctg gttgttgttc ttgttctgta tgtatataaa cactatcgac atattatgac
     3001 cgggaacatt ccttaccccc tctgcgctcg cagaggtttt ttttgtcatt aattaataat
     3061 gaactgaaat gcagttccga tacctccatt tgcacattat ccctgacata tggtaccgga
     3121 accgcattaa ttttatgtta taagagttta tttttaatgt ccagatggtt taatagtatt
     3181 tatatgtaat tagctgtatg gatagtactg gttttattgt ataacttgtc gttttttttt
     3241 attgtttttg tgtggtgtgc taatctttaa gttataaaaa aaccccttgg aatacagggg
     3301 caaaagtaag gaaaacaaat ggatgctcag tttgtgtgac actttaaact aatggaacta
     3361 tagagtggag gttgttacag tcgcttttat aaaacagcaa ccagtttttt tcaatagtct
     3421 gatgatggtt gtgacagttt tgtgatattg ttcaaaatgt ttcttgttga atgtttgttt
     3481 tttggtctat tggtgtctgg ttttattttt tctgctatta catatcaaga aatgtgattg
     3541 gtgtagcaag agtggaatgc gatgtggata aactaattgg ttgtcgaatc taatttactt
     3601 agaaaactac acttgttttt gtgtatgaat ttagtgaatt tgtctgtcta tgtgttttcg
     3661 tgcatttata aatccttcgt caggatctta aatttagtgt tgaatatgta gggcctgtac
     3721 ggtaattatt atttagcgta gctacttcat cgttgttcga acacaaccaa caacctgtaa
     3781 ttgttgtttc tcttggttcc ccacttttgt ggggtttttt atctcttccg tgcatcgtaa
     3841 agcgcggact ccttctggaa aggttgtttt agggcatcac tgaggcattt tttgcgtttg
     3901 tgaatattga gcatccgata actaaaatat ttcccataga ctctgattct tgccaccgac
     3961 cccaggtcgg tttttttttg tcctggcgag atgtgaagtt tggaaacagg aaaccccgtc
     4021 agagcgccac gcaccagggg agaaaacttg tcgttttgag ccgttcggtg ttcagaacgc
     4081 acgaaaccga tcgcacatcg ctttcgtgca tagttatgca acgccctgaa aacgattctg
     4141 acgcgttttt ctggtttggc ctggtgtttt cttgtctttt tgcgtttttt gcgccagaaa
     4201 gcgactgagg gcgttttaag ggggcgtaca acgggagtta tggtaaatgg atcgggtttt
     4261 cgggaaggtt cgacaggatt tgccgttggg tgtagtgtaa gcgactgaaa aacaaacgcc
     4321 cctgaaatca tgtccagatc cggcaagatt caacatgaaa tacagagggc gtgtaggtcg
     4381 cataacccgg agagaatcgt aacacatgag cgccgcgctt caatacttcg acgaaaattt
     4441 accccatcgt ccctatcaca cggatgatct cgcttttggt cttcgcatct ccggcaaagg
     4501 gcgtgcgctt cttgcgcggt acatacagca gaaccagcct catgcgcagt tctggctggt
     4561 ttttgacgtt gaccgcgagg gggctgcgat tgactggagc gaccggaacg ctcccgcgcc
     4621 gaacatcacc gttaaaaatc ctgtgaacgg acacgctcac ctgctctacg cactcaacat
     4681 cgccgtgaga accgcgcctg atgcctcggt taaggcgctg aaatatgccg cagcggttga
     4741 acgttcgctg tgtgagaaac tttgtgcgga tgtgaactac agcggcctga tttgcaaaaa
     4801 tccgttccac ctggaatggc tggtgatgga gtggcgcgag gaagcctata ccctcgatga
     4861 actggctgat tatctcgatt tgagcgcctc agagcgtcgt agcatcgata aacattacgg
     4921 gatggggcga aactgccacc tgttcgaaat gacgcgcaaa tgggcttaca gggcgattcg
     4981 tcagggctgg ccagcgttct cacagtggct tgaagcggtg atccagcgtg tcgaaatgta
     5041 caatgcatcg cttcccgttc cgctttcact gg
//
