LOCUS       NZ_CP124336             4073 bp    DNA     circular CON 07-JUN-2024
DEFINITION  Escherichia coli strain AVS0786 plasmid pAVS0786-B, complete
            sequence.
ACCESSION   NZ_CP124336
VERSION     NZ_CP124336.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34375059
            Assembly: GCF_029991165.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Biggel,M.
  TITLE     Genomic characteristics of ESBL-producing Escherichia coli ST131
            isolated from the water ecosystem in a defined region in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029991165.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 03:34:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,127
            CDSs (total)                      :: 5,017
            Genes (coding)                    :: 4,778
            CDSs (with protein)               :: 4,778
            Genes (RNA)                       :: 110
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 97 of 239
            Pseudo Genes (incomplete)         :: 173 of 239
            Pseudo Genes (internal stop)      :: 52 of 239
            Pseudo Genes (multiple problems)  :: 70 of 239
            Pseudo Genes (short protein)      :: 1 of 239
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124336.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AVS0786"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pAVS0786-B"
                     /geo_loc_name="Switzerland: Kanton Zug"
                     /collection_date="2021-08-18"
                     /collected_by="Amt fuer Verbraucherschutz, Kanton Zug"
     gene            complement(join(3948..4073,1..576))
                     /locus_tag="QJP89_RS25575"
                     /old_locus_tag="QJP89_25575"
     CDS             complement(join(3948..4073,1..576))
                     /locus_tag="QJP89_RS25575"
                     /old_locus_tag="QJP89_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1110..1364)
                     /gene="mobC"
                     /locus_tag="QJP89_RS25580"
                     /old_locus_tag="QJP89_25580"
     CDS             complement(1110..1364)
                     /gene="mobC"
                     /locus_tag="QJP89_RS25580"
                     /old_locus_tag="QJP89_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1543..3690
                     /gene="mobQ"
                     /locus_tag="QJP89_RS25585"
                     /old_locus_tag="QJP89_25585"
     CDS             1543..3690
                     /gene="mobQ"
                     /locus_tag="QJP89_RS25585"
                     /old_locus_tag="QJP89_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_032495499.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGKSGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(1366..1542)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1543..1901
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..576)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1457..3687
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggcttgatt atcatcttat tcaagtcttt gaattgttga tatttatctt caacggcaag
       61 catggttcgg aaatcatcga tagctatcgt tctgtcaccg gtgctgcgga actgctggag
      121 cagctcgtaa atgcggatgc tgtaggtgct ggaaaggctg gagacgttct tcacgactac
      181 ccgcgtaaac tgccccttga gctgtgtcag atacggcatg atggcatcgg agaaggtgat
      241 atctactttc gcctcacctt tgaaatactg cgcccggtac tgcacccagc ggaactcttc
      301 tcgtttttcc tcgttcttga tgattactga cctgtcccag aggcgatcta tcgcttcctt
      361 gagcctagct tcagcgtgct gccgtcccat gtcaggaaag gcatcatgaa actctgcagc
      421 cgtgatggtc atggtttttt gttcatccgg cgaaggggaa tcttctccgg attttaggcg
      481 gctgatacag accagcagga agcgctgttc ctgcaaagtg agcttgtagc ttgcctcgat
      541 aagggcgttt gatttgtaag ctatcagatc tgacatttcg cttgctcgtt gctattggtg
      601 gcgttttttt actgtacgcc acttatcata tgggtggaag tttttttttt gcaaccccct
      661 ttttctgtgg attacacccc caaaagctcc acctttcacc cccaaaagct ccacctttca
      721 cccccaaaag ctccaccttt cacccccaaa agctccactt atcttgccag aacatcttta
      781 aaatcatggt gttacaagac ctaaaaaggt tttaaaaaaa aagatctaaa aataagatct
      841 taaaaactta acggcgagcc tgtggataac ttggggtgct gaaaacaaaa aaccaataaa
      901 aacaatgcgt tgcaataaag cagaattgca agtttcgaat acaccccctc caaccctgtg
      961 gaaacccagt attcatgcgg gttcagcgca tatcacctgc cgaaaaaccg tgcgtatgtg
     1021 ctcaacctgg cgactgattc acttccgctt cctcgcccac tcgctcgcta acgctcggtc
     1081 gctggggctg cggcgatcgg acgatacaat cactgttctg gcagcgcctc cagctcgtta
     1141 atcagcggca gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt caggaactgg
     1201 agtaaccagg cacgcaactg cacctcctcc ggctttctga cctttgccag caatgcagca
     1261 cccacaacaa ccttctgcgc tgtgtccagg ctccggctct tcgccttcag gcgctgtaat
     1321 ctcgcctcag catcagcaat cttctgttcg agcgttctgc tcatgatgta actccgtgcg
     1381 cgatgaaaaa tcgcatttta gcgcgtcact ggtagtttaa aaactgagct ggcataatgc
     1441 acggcaaatc acgaagtgcg cacttataca atctccactc cgtttcgatt gtgtgcgccc
     1501 tgcggggctg aaagaaaact acaaaaaggc attacggcag aaatggcgat ttatcatctc
     1561 agcatgaaaa ttatctccag aagcagcggc tacagcgccg tagcttccgc tgcctatcgt
     1621 tccggttcac tgatgcttga tgagcgtacc ggactgactc acgactacac ccgcaaaagc
     1681 ggtgtggctg aggctgtgat tctgaccccc gccaccgccc ctgcctggtg tacgaaccgt
     1741 gccgaactct ggaatgccgt tgagaaggcc gaacgccgga agaactcaca acttgcgcgg
     1801 gaaattgaac tcgccatccc ccacgaactg ccgcaggatg ccgcacggga aaccgttctc
     1861 gcctttgtca gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc gttccatcac
     1921 atggataaaa caaatcccca tgcgcacatc atgctgacca cgagggctat cggacctgct
     1981 gggttcagcg gaaaggtcag ggactggaac gaccggacac atgccgagac gtggcgggca
     2041 tcatgggctg accatgcaaa cagggcactg gcgaacgccg gttatcagga agagatagac
     2101 catcgttcat acgagcgaca ggggcttgag aaaacgcctg gtattcacct cggaaagtct
     2161 gcctgtgcga tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt
     2221 aacaggctta acctggaaat acagatttcc cgcaccgaac tcaggaacgg agggctgaca
     2281 gagcctgccc gccgtgtggc tgaccttctg aatatcgggc ttcctgacaa cgcaacttcg
     2341 tacacactac gtgacattat cgaagcgttg ccgaaggaca gtaactcggc atggaaactc
     2401 accagtaatg ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag
     2461 ctgaacaccg aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg
     2521 ttttcgtccg gtttttcccg tattcccctg atgcaatggg cggcaccgga ataccgtagt
     2581 gagcagggaa aaatccagtc tctgagccag caaatggaaa aattacgaca gcattaccgg
     2641 aaggtggaga aacaggacat tcccgcgagt cagatggcat ttgaaaaaca gtggcagcag
     2701 tgggggaaat ccggccttgc agctctcaga gcccagttgc ttaaacgtga agaggaattg
     2761 cgccggaagg aacaggaaga tactgaacgt agtcgcgcgg agcagctcag gaaaaatgac
     2821 aatgccgtta tggattcagg ggtgctgaga gcagtcgtta cggggtatgg cgaggctccc
     2881 atgcctggta acggggaaat gacctgttac cttctgcttc acaaccggaa cggagaatat
     2941 acgctctggg ggaacgagct ggagaaatac cgcaccagaa tctttgagag tgttgattta
     3001 atgcgtgaca ggagtggtta tatctgtgac cgaagcgaaa tcggccaacg tccgccactg
     3061 caacgtgttt attcctctgc aacctttgag caactgctga cgcaggtttg ccagacatgg
     3121 ccgcaacata cccgcaatct ccgccagccg aagacatggc cggagtcttt ctgtcttgga
     3181 gaagaccgcc agccagccat gccatcacca gcggcaagga aggtggattt tatgcaggga
     3241 cgtctgccac cgacgctgat gccggtcatg agttctgtcg accgggaaac ccgccagctt
     3301 cagcttttgc tggtcatggg cgtggatgac tctctgggcg gtgttatccg gcttaatggt
     3361 acgctgtatc ccgcgtttgc cgttccctca gccgataaca ggcatctggt catcagtgcg
     3421 ctgaccgata aggggttacg gtatgcaggg tatggtgttg ccgtaaatca cgatgcagac
     3481 agccacatat cacctgcacc agaactgatg cagtttcatc tgaaaacccg tgaggcaccg
     3541 ctttttgctg ccgtcaacac accagaaaaa cagccggatc atctgttccg ctcgctgggt
     3601 tttaatcgta cctgggagga atggcagcgc gaggaagatg cacgaacaca caccaccgag
     3661 aggagacatg atcgcggttg gtcacagtga atgtgaacaa tattttgttt tgtttaacat
     3721 cgtgttatgt tcttcgatgt tcttcgatgt tcatcgacag ggatgaatgc aagaagcaca
     3781 ctcacataac gcaagcatag ttttgttttt ccgcccccaa aaattggggg gtcttttttc
     3841 gctgcatcag agcgtttttg acgcaatttt tcacttcaat agtaccttcg cactactaac
     3901 acaaaaaaaa cgcagcaggg agcgttttag gtgccttaaa atcgatttta gatcgtcatc
     3961 ttgatctgtt tgtcttcctt gaagcgaaaa tgcagggcta caaccgtgcg cccctttttg
     4021 atcgtctcaa cagttacaac cagatcgctt ttttcgttca gttcggcgat ggc
//
