LOCUS       NZ_CP124125             3815 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00198 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP124125
VERSION     NZ_CP124125.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907133
            Assembly: GCF_029948145.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00198
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029948145.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 05:32:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,887
            CDSs (total)                      :: 4,769
            Genes (coding)                    :: 4,700
            CDSs (with protein)               :: 4,700
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 40 of 69
            Pseudo Genes (internal stop)      :: 12 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            Pseudo Genes (short protein)      :: 1 of 69
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP124125.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00198"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2019-12-02"
     gene            complement(320..535)
                     /locus_tag="QIW35_RS24390"
                     /old_locus_tag="QIW35_24390"
     CDS             complement(320..535)
                     /locus_tag="QIW35_RS24390"
                     /old_locus_tag="QIW35_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(542..1033)
                     /locus_tag="QIW35_RS24395"
                     /old_locus_tag="QIW35_24395"
     CDS             complement(542..1033)
                     /locus_tag="QIW35_RS24395"
                     /old_locus_tag="QIW35_24395"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012564908.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAVFSEHERSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<1068..1772)
                     /locus_tag="QIW35_RS24455"
                     /pseudo=""
     CDS             complement(<1068..1772)
                     /locus_tag="QIW35_RS24455"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1711..2034)
                     /locus_tag="QIW35_RS24405"
                     /old_locus_tag="QIW35_24405"
     CDS             complement(1711..2034)
                     /locus_tag="QIW35_RS24405"
                     /old_locus_tag="QIW35_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3189..3530)
                     /locus_tag="QIW35_RS24410"
                     /old_locus_tag="QIW35_24410"
     CDS             complement(3189..3530)
                     /locus_tag="QIW35_RS24410"
                     /old_locus_tag="QIW35_24410"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_074184457.1"
                     /translation="MAKPPSATPPDTPSSCDVSALPGIIQNFGLINFNVSCNNGELTVT
                     GTDQNGKHIRVNSYQGNGFTEGTMSTFQPGGKEARRLEAVRLRESGLTQQEIANRLGVS
                     QKTISNDLK"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2148..2321)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.714"
                     /coverage="100.0"
                     /evalue="5.6599999999999995e-68"
     Typing          2288..2796
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(229..1721)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaagtcacac taaaatccgc gccaatgcct taaaaaccga caaatgcttc tccagccagt
       61 cccacagctc cgccgccaga tacaggcagt taagcaatga ttcaataaga tttaacagct
      121 tcatttcccg ctcctgaata agtcgtgacc tttttgatca cgaacctatt tagcgggaga
      181 aataagagcc cctcttcgtg cgggttgtca gctgcctgga tggtttaaag tgtcggcccg
      241 cggtaggttt tctgcggctg gcgctccagc tccagtgttt tctgatactc attctccctc
      301 tgccggaact cggtggcatt cagttcccgg acaagcggcg cagctgctcg cttaaactca
      361 gcacctgcgt gctcaagtcc gacacgcgct cgttcagcac cgcgttctcc cgttgcataa
      421 gaccacacat gctccgccat tccgcgaagg cgctctccca ttcgtccagc ctttgcgagt
      481 agtcctgctg tagctgctct aatgcgctca gcaactgctt ttccagctct gtcattttcc
      541 gctactcctg tttcaggcgg atccatgtcg tcccgtcgta ggggatggct tcggtttgca
      601 tccccggcgg caggataaga aacctccggc cgtcgctgct ctcctgatag cgcactcccc
      661 acgtccgggc ggtcattttc gccagggtat cttcctgctg acgaagatgc gtgtaattgt
      721 cggttatctg ctgcccctgc caccacagaa tgctcccgct cgtgccgatc agcagcgctg
      781 acaccatcag gatggtgagc catgtccgcc cggcagtccg cagtacgctc cgccggtttt
      841 tctccatcgc ctcgctcata ctctgctcgt gggcgaggat ggcgtcgctg attctcctcg
      901 cgcttgcgct cagttccgct ctgacagacc gctcgtgctc gctgaatacg gctttcagca
      961 tctcgccggt actctgctgc tgcgctttcg attgctgctc taagtccttc gccagcgtta
     1021 aaagactgct catagatggc tcccttcagt cggatgtttc gccccccgtc cgggtcggca
     1081 atgctgatac tgcttttcgt ggtgcgcacc acctcaaaac ctgcaccttc aagagtgtca
     1141 gtgacatcct gacggctttt cagctccccg gacgaagcaa gggtcagcag tccccgggta
     1201 atggcttctg cggcctcctg cttcgttttc ggcagcccgg acggcgtgac cagcgcccgg
     1261 cggttttccg gcgcgttcgg gtcgtgcagc cccagtctgc cgttcacgat agtctgccag
     1321 gcatcgatgc gcggccggtc ggcgcggtcg tagtaaggct ggagccgcct gccggtcagc
     1381 agctcggtat tggggatcag aaagttcagc tccagacgcc ccttgtccgc gtgctccacc
     1441 cacagaatgc tgtactgatc tttatcgagt ccgggcatca gaacccgctc gaagctggcc
     1501 atcagttttt cccgctggcc gggaggcaga tcggcctccg caaacgacag aacgccggag
     1561 gtgtatttct taacgtaagg cgacgcgtca atcagctccc ggacttcttc cggcttcccc
     1621 tgcaggacgg tggcgccttc acgctgccgg tctttaccca gcagatagtc caccggcccg
     1681 gcgccaccgc cgcggccgcg cggatgaaat ttaacgatca tcgtccgttc ctttctcaag
     1741 aacggcgtgc cgcagccgct caaggccggc atcgatggcc atcagcgccg ccacaatctg
     1801 taccctgtca tggccggtgc cgccgccggc gttaacacgg cgggctatct ggttcaggtt
     1861 attgcccatg ccggcaagct ggcgcagcag cgccggcgag agtgagggga gctttccgga
     1921 acgtgccggc ttttcgtcca ggcagatctg ccgcatccac gccgccagct gcctgccgtc
     1981 acagcgctcg agcagccgcc ggtgctcgtc ctccgtcacc cacatggtga gcatcttgct
     2041 ccgtttctct gacaaaccgg cctcctgata acgccgggac gggcgggaaa gcctttcccg
     2101 gtcggcgcgg ggttggacaa gccgcaggcg cgtcaggctt ttggcgggtt tcggggcgca
     2161 gccctgaacc agtcacgtag cgctagcgga gtgtatactg gcttactatg ttggcatgga
     2221 tgcggaaatc agtgaagtgc ttcatgtggc aggagaaaaa aggtggcacc ggcgcgtcaa
     2281 caggagatgc aatacaggag atattccgct tcctcgctca ctgactcgct gcgctcggtc
     2341 gttcggctgc ggcgagcggt accggcttac gaatggggcg gaaatttcct ggtagatgcc
     2401 aggaaggaac ttaacaggga aacgataagg ccgcggcaaa gccgtttttc cataggctcc
     2461 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
     2521 gactataaag ataccaggcg tttccccctg atggctccct cgtgcgctct cctgttcctg
     2581 cctttcggtt taccggtgtc actccgctgt tatggccgcg tttatctcat tccacgcctg
     2641 acactcggtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     2701 cagtccgact gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacac
     2761 gaaaaaacgc cactggcagc agccactggt aacaggatta gcagagcgag atatgtaggc
     2821 ggtgctacag agttcttgaa gtggtggcct aactacggct acactggaag gacagtattt
     2881 ggtatctgcg ctctgctgaa gccagttacc aggttaagca gttccccaac tgacttaacc
     2941 ttcgaaaaac cgcctcccca ggcggttttt tcgttttcag agcaagagat tacgatgcaa
     3001 tggaaaggat ctcaagaaga tcattattga tgttcgacat atatggggat ctgcaccatg
     3061 gcttaaaata aacatctgaa atgtatcttt aggtgtttca ccactgcggg acgagcaact
     3121 acgccggatt tatcatggca tttcagtagc gatgaaaagc ccgttaagac gggcttaaag
     3181 caagtgactc attttaaatc attgcttata gttttttgag aaacgcctaa ccggtttgct
     3241 atttcttgct gcgtaagccc actttctctt aaacgaacag cctcaagtct tctggcctct
     3301 ttacctccgg gctgaaacgt tgacattgta ccttcggtaa agccgttccc ttggtagctg
     3361 ttaactctta tgtgtttccc attttgatcc gtacccgtta ccgttagctc accgttattg
     3421 caggatacgt taaagttaat taagccaaaa ttttggataa tgcctgggag agcactaaca
     3481 tcacatgaag atggtgtatc cggtggtgta gctgatggag gttttgccat tgtgcgttcc
     3541 ttgattttta cgatgcatcc atactagtaa atactagaaa actagtaata tcaataatcc
     3601 atatcccttt aggccatcac cgaagcccct gcttatagtc acttctcccc ctacatttac
     3661 cataacatcc gttgcccgca ccgctgaaac ggggttggct gcgtttccgg tgccagacgc
     3721 gagaaagtgg cgacagacat aacgaaaaaa caccagaaac gcagccgctg aatattacac
     3781 tggtgcatag tcatgcagcc agatgcatgg atgtt
//
