LOCUS       NZ_CP123927            10047 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Salmonella sp. SA15303 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP123927
VERSION     NZ_CP123927.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33419349
            Assembly: GCF_029962125.1
KEYWORDS    RefSeq.
SOURCE      Salmonella sp. SA15303
  ORGANISM  Salmonella sp. SA15303
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Zhao,w.
  TITLE     Oxford Nanopore Long-Read Sequencing Enables the Generation of
            Complete Bacterial and Plasmid Genomes without Short-Read Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Zhao,w.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2023) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, Changbai Road, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 11:56:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,944
            Genes (coding)                    :: 4,810
            CDSs (with protein)               :: 4,810
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 38 of 134
            Pseudo Genes (incomplete)         :: 104 of 134
            Pseudo Genes (internal stop)      :: 25 of 134
            Pseudo Genes (multiple problems)  :: 28 of 134
            Pseudo Genes (short protein)      :: 1 of 134
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123927.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Salmonella sp. SA15303"
                     /mol_type="genomic DNA"
                     /strain="SA15303"
                     /host="Homo sapiens"
                     /db_xref="taxon:3030528"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Beijing"
                     /collection_date="2022-10-28"
                     /collected_by="China Institute of Infectious Disease
                     Control"
     gene            complement(<79..261)
                     /locus_tag="QEN31_RS25265"
                     /pseudo=""
     CDS             complement(<79..261)
                     /locus_tag="QEN31_RS25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            423..1037
                     /locus_tag="QEN31_RS25270"
     CDS             423..1037
                     /locus_tag="QEN31_RS25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412822.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_014839983.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKREE
                     RPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTT
                     TAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMG
                     QSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
                     /name="recombinase family protein"
     gene            complement(1356..2012)
                     /locus_tag="QEN31_RS25275"
     CDS             complement(1356..2012)
                     /locus_tag="QEN31_RS25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="WP_001516695.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFVN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRTNF
                     AHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS1"
     gene            2407..>3437
                     /locus_tag="QEN31_RS25280"
                     /pseudo=""
     CDS             2407..>3437
                     /locus_tag="QEN31_RS25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            3423..3728
                     /locus_tag="QEN31_RS25285"
     CDS             3423..3728
                     /locus_tag="QEN31_RS25285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_223229636.1"
                     /translation="MPKPDNSCCRSEDIVCRFGGEEFVVFLPNTSVVTAECVAERIRSV
                     IESTVFPNNLRVTVSAGVATQEDPLGGIDFLLKNADDALYQAKGEGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     gene            complement(3971..4129)
                     /locus_tag="QEN31_RS25290"
     CDS             complement(3971..4129)
                     /locus_tag="QEN31_RS25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001658417.1"
                     /translation="MHEPPVQSDCCASSGNFQLDSNPERHDKAPLAAAIGNWELVDLDI
                     ESLEVVA"
                     /name="hypothetical protein"
     gene            4779..5102
                     /locus_tag="QEN31_RS25295"
     CDS             4779..5102
                     /locus_tag="QEN31_RS25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015059464.1"
                     /translation="MLTIWVTEDEHRRLLDRCDGKQLAAWMRQTCLDEKPARAAKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5311..>5745
                     /locus_tag="QEN31_RS25300"
                     /pseudo=""
     CDS             5311..>5745
                     /locus_tag="QEN31_RS25300"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5780..6265
                     /locus_tag="QEN31_RS25305"
     CDS             5780..6265
                     /locus_tag="QEN31_RS25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012482081.1"
                     /translation="MSSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELNESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSVLLIASSAGILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRSCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            6269..6484
                     /locus_tag="QEN31_RS25310"
     CDS             6269..6484
                     /locus_tag="QEN31_RS25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            6790..8166
                     /locus_tag="QEN31_RS25315"
     CDS             6790..8166
                     /locus_tag="QEN31_RS25315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900944.1"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="WP_080196049.1"
                     /translation="MVNVEFFNYIECIFMRIKKPHLRPIVITLSVGGILLTSIFLIVVI
                     MVFKRENIEKDLLDANSSYAMKMSDVMSNYIEIAQRELAYGAKKIESTNDIDSLKNEAD
                     RLRLQSGMFNSVLVVSDGAVVLATSPESLGLVGVHLNSSASKLAIEAKKPFISAPYKSA
                     SGNLIIFLSHPIYDKNSNYIGYVGGSIYLKEQSLFSDVLSRHFFKSDTEISIVSDDGNV
                     IFNNDNSAVGRPMVMDDNLKSKLTSSERGEGIFFSSGNKYLLGYAHMKNTAWSVLVYSH
                     ADSVTTILITYAKSVLVLLIVIVITFSLFSYFIASRISIPLEKLAISTNAKDIGGSLVD
                     IKGINAWYVEADRLKKALFTHIKIMIERVDSLNEVALKDPLTGVNNRLGFSNKIQNHKQ
                     GSGASVIAIDVDFFKKINDNFGHGVGDEVLVTLAQVINCGFPGLGRFPQSRGQPERKPE
                     "
                     /name="diguanylate cyclase"
     gene            complement(<8094..8699)
                     /locus_tag="QEN31_RS25320"
                     /pseudo=""
     CDS             complement(<8094..8699)
                     /locus_tag="QEN31_RS25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /name="ISKra4-like element ISKpn19 family transposase"
     gene            complement(8736..9308)
                     /locus_tag="QEN31_RS25325"
     CDS             complement(8736..9308)
                     /locus_tag="QEN31_RS25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001493762.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNRP
                     GLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGTNN
                     PMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALRSRG
                     VRLRIACDHAGLGVSTYYKLRHQMNNQ"
                     /name="recombinase family protein"
     gene            complement(9445..9990)
                     /locus_tag="QEN31_RS25330"
     CDS             complement(9445..9990)
                     /locus_tag="QEN31_RS25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_001493763.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGIS
                     YEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFCIS
                     GHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQLSRL
                     DLASPVLEPEVIWLGRRR"
                     /name="plasmid pRiA4b ORF-3 family protein"
     ARG             complement(1360..2012)
                     /name="qnrS1"
                     /gene="qnrS1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(3963..4506)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.788"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          5093..6588
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.583"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgttatgaa atgatggtgg ttattttaac ccctgtagat ggcatcattc gatggctgcc
       61 ccgaggtttt tcatgctctc cgatctggat gatgcaataa atttgagcgt gaagcccccg
      121 gcgactgcag gccagcgcca gttcatatcc attcagcgcc agaaactcca gccgctgtgc
      181 atcatcgaaa tcaggtagac catagagcgc ttcctgctcg gcgtccgaaa gaatactgag
      241 gagcttattt ttagtaggca tctctgatcc ttatgcctat ttaaacgcgc ttgtcacatt
      301 atcattaaca tagaaccttt acgtgtcata atttaaatta cgggtaatgt gactaccgtt
      361 gacagattat ggtacccaaa tcagaatgtc acataatcga acgtatatgt gacagaggaa
      421 atatgttcat cggatatatt cggatgtcaa aagctgatgg ttcgcagacg aatgatttac
      481 aacgtgatgc actgatcgcc gcaggtgttg atgccgcaca tttttatgag gaccgggcat
      541 cgggcaaacg agaagagcgt cctggactta cagcctgtct gaaagcacta cgacccggag
      601 acacgctggt cgtatggaag ctggaccgcc tcggtcgtga tcttcgtcat ctcattaata
      661 cagtgcacga cctgaccgcg agggggaccg gtctgaaagt gctgaccggt cacggggcga
      721 ctatcgacac aacaacggcc gctggcaagc ttgtcttcgg tatctttgcc gcactggcgg
      781 agtttgagcg cgaactgatt gctgaaagaa cgactgcagg tctggcatca gcaagagccc
      841 gtgggcggaa cggcggccga ccttataaaa tgacgccagt caaactgcga ctggcaatgg
      901 catcaatggg acaatcagaa acaaaggtca gtacgctatg ccaggaactt gggataaccc
      961 ggcaaacttt gtacaggcat atttcccctg ttggtcaact gcgggcggac gggatcaagc
     1021 tgttcaaccg tggataattt gaacggacgg aaaaatctgg ggttccggcg tagtacccct
     1081 atcacctcca catttttgca acagtgcctt ataaatggca gccttatgtt tacttgccaa
     1141 gatgggctac catatgatgt tttatactga gcaatattaa tattatcttt aatggaaaaa
     1201 ttaagcccac taagtgaccc actgctatat ggctctattg tgaagacgcc tgagggtaaa
     1261 aatttataca tggttgtccc tatgtctatt attgcaaggt tgacaatatt attcgtttta
     1321 taaatttgag cgtttaaaat cacacatcaa agcaattagt caggataaac aacaataccc
     1381 agtgcttcga gaatcagttc ttgctgccag gctgcaattt tgatacctga tgtatcgact
     1441 ttgcggggat ctaaaccgtc gagttcggcg tggcataaat tggcaccctg taggctaaat
     1501 tgcccccaga catcttcgga aaaaacacct cgacttaagt ctgactcttt cagtgatgca
     1561 cccgctaggt tcgttcctat ccagcgattt tcaaacaact cacatttttc taaacaaacc
     1621 ctctccatat tggcatagga aagattacat ccagaaataa atgctgagca aaagtacata
     1681 cgattactca cttgatgggc aaagtttgtt cgggaaaagt tggcaccttt taaatcacac
     1741 gcacggaact ctataccgta gcaattggca ttactgaagt ttgccatcgc aagttggcat
     1801 tgttggaaac ttgcatcacg aagatctgcg acatcaaagt ggcagccttc gatatcaccc
     1861 tgttcaatga acttgcagtt gacgaatgtc gtatcacgca agttagcacg tcgaaagtcg
     1921 ctgcgaatga atgtgcaagc ggtgaaggtg agatcactta agtctttatg tgaaaagttg
     1981 tggtgccgat atgtatgatt gtaggtttcc atgagtactc cttgttgaaa ggagggctag
     2041 actaccatta tacatatgcc aagacattaa aataaaagcc tgttacatca acacattaca
     2101 acccctcaat ttacctgttt taagtggtca aattggcgcg tagagcgcct atcgctcttg
     2161 cattggcacg cggccctgtt gaaaaaagtt attcgcttta gatagggtta ggagagccgt
     2221 gcttcgccgg taaaaaatcg gactatccga cgcagggcgg actcatcggc ggagaagcaa
     2281 actttgacta tatctcccta cacttggaag agggctagac tggccccata gatcaccaga
     2341 cacttcgact ctcttaaaat aagaggtagt cttggaacta tgtttaatac cagtcagaac
     2401 gataagatgg ttgaagtgtt atcaggacct gagcggcgtc ggcgtcgtac accgcaggaa
     2461 aaaattgcca ttattcagca gactatggag cccggtatga ccgtgtctca tgtcgcgcgc
     2521 ctgcatggca tcaatgcaaa ccagatattc aaatggcgca agcaatatta agacggttca
     2581 ctgacggctg ttgcatcggg cgaggaagtg gtgccggcct ccgaactcgc tgccgccaat
     2641 aagcaaattc gcgaactcca gcgccttctg ggcaaaaaga cgatggaagc cgagatactc
     2701 aaagaggccg tggagttcgg ccgggcaaaa aaatggattg cgcatgcgcc ctcgttgcct
     2761 gggaacgacg aataagcgac gtttgcagaa gtcttggcgt gtcgcgtgcg cagttgagca
     2821 ttcgggttca ccggccatct gactgacagg atcgcagacg gcagccccgt tctgacgata
     2881 ccaccgtatt gtcccggata aacatggcgg tggctgacct gccgacgtat ggttatcgcc
     2941 gtgtgtgggc gctgctgcga cgggagtcgg agcgggacgg cctgcctgtg gttaatgcaa
     3001 agcgggtgta tcgcattatg agaacgcata atctgcttct tgaacgtaaa cccgctgatc
     3061 cttgccgtaa gcgggcccat aaggggcgcg tagcagtagc ggaaagtaac cggcgctggt
     3121 gctcagatgg ctttgagttc cgctgtgata atggcgaaaa gctgcgggtg accttcgcgc
     3181 aggactgctg cgacagggag atcattgatt gggcagcgag caccggaggc tatgacaaag
     3241 aaacggtgca ggatgtcatg ctgggtgcgg tggaaaaacg cttcggaagg cagttgccaa
     3301 cagtgccggc gttcgcccgg ttgctgggac tggagccttg tacgacagca gtgcgaagcc
     3361 cggaaagcaa tggcatagca gaaagcttcg tgatgacgat aaaacgggac tacatcagca
     3421 tcatgccgaa accggataat tcttgctgcc gtagtgaaga tattgtttgt cggtttggtg
     3481 gagaagagtt tgttgttttt ctgccgaata catctgtggt aacagcggag tgtgttgcgg
     3541 agcgtattag gagtgttatt gaaagtactg ttttccctaa taacttgcgg gtcaccgtct
     3601 ctgctggtgt ggcaacacaa gaggatcctc ttggtgggat cgattttttg ctgaaaaatg
     3661 ctgatgatgc tctttatcaa gccaagggag aggggcgtaa taaagtgatt gtatatagcg
     3721 ctatttgagt ttttttgatg acgctttacg cgctagtgtg gcgttttttt gtggggaaaa
     3781 gggttcaaga agcggatcct cttgttatcc cttcgatgtc gtcgtaagct catgccctgt
     3841 aaacaaaaaa accaccgcta ccaacggtgg ttcgtttgcc ggaattaaga gctaccaaca
     3901 ctttcttccg aaggtaactg gctttagtgg agcgcagata ccaaatactg tcctttcagt
     3961 gtagccgtag tcaggccacc acttcaagac tctcgatatc taaatccact aattcccagt
     4021 taccaatggc tgctgccagt ggcgctttgt cgtgcctttc cgggttggaa tcaagttgaa
     4081 agttaccgga ggaggcgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct
     4141 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     4201 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcta
     4261 ccagggggaa acgcctggga tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     4321 cgtcagattc tgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctctgccgtg
     4381 gccttatcgc ttccctgtta agtttcttcc tagcatcttc caggaaattt ccgccccgcc
     4441 cgtaagccgg taccgctcgc cgcagccgaa cgaccgagcg gagcgagtca gtgagcgagg
     4501 aagcggcatt tctccggcag tgcatcttct gctgacgcat acgaatctca tttttcacct
     4561 gctttatgtg gcagctatgc ttgatgtttc tgcgatgtta cattttaagc cagtatacac
     4621 tccgctagcg ctacgtgact ggttcagggc tgcgccccga aacccgctaa aaggcactga
     4681 cgcgcctgcg gcttgtccaa caccgcgccg accgggaaat aattccccgt ccgtcccggc
     4741 gttatcagga ggccggattg gcagacaaac gcagcaaaat gctcacgata tgggtaactg
     4801 aagacgagca ccggcgtctg ctggatcgct gcgacggtaa gcagctcgcg gcctggatgc
     4861 ggcagacgtg tctcgacgag aaacctgctc gtgccgccaa acttccgtcg atctcgccgg
     4921 cgttgcttcg tcaacttgcc ggtatgggaa acaacctcaa tcagattgcc cggcaggtta
     4981 acgccggtgg tggtaccgga catgatcgcg tacagattgt cgctgcgctg atggccattg
     5041 atgccggact tgagcgcctg cggcacgctg tgctggaaaa gggggctgac gatgatcgtt
     5101 aaatttcacc cacgagggcg cggcggcggt gccggtccgg tggattatct gctgggtaat
     5161 gaccgcaagc gcgacggcgc cagcgttctg cagggtaaac cggaggaagt ccgggagcta
     5221 atcgacgcct cgccctacgc caaaaagtac acctccgggg tgctgtcgtt tgccgaacag
     5281 gacttaccgc ccggtcagcg tgaaaaactg atggcgagct tcgagcgtgt tctgatgccc
     5341 ggactcgata aagaccagta cagcgtgctg tgggtggagc accaggacaa ggggcggctg
     5401 gagctgaact tcctgatccc gaacacggag ctgctgaccg gcaaaaggct ccagccgtat
     5461 tacgaccgcg ctgaccgtcc gcgcatcgat gcctggcaga ccatcgtgaa cggcaggctg
     5521 gggctgcacg acccgaatgc gccggaaaac cggcgggcgc tggtcacgcc gtccgcgctg
     5581 ccggaaacga agcaggaggc agctcaggcg atcacccggg gtttgctggc cttagcctcg
     5641 tccggggagc tgaaaacccg tcaggacgtt actgaggcgc tggaaaacgc gggttttgag
     5701 gtggtacgca ccacgaaaag cagtatcagc attgccgacc cggacggggg gcgaaacatc
     5761 cgactaaagg gagccatcta tgagcagtct tttaacgctg gcgaaggact tagagcagaa
     5821 atcgaaagcg cagcagcaga gtaccggcga gatgctgaaa gccgcattca gcgagcacga
     5881 gcagtctgtc agagcggaac tgaacgaaag cgagaagaga atcagcgccg ccatcctcga
     5941 ccacgaccgg aagctgtcct cagccatgag ccagcgcacg aaaggcatgg tgcgtatggt
     6001 cagccagacg tggctgacca tcgtcctggt ttccgtcctg ctgatcgcat cgagcgcggg
     6061 cattctgtgg tggcagagtc agcagatact cgacaattac acgaccatcc gggagcagaa
     6121 gagcacgcag gcgatgctgt ccgagcggaa cagcggcgtg cagctctcga cctgcggcga
     6181 gcagagacgc agctgcgtga gggtgaaccc ggaagcggga cggttcggag aggattcgag
     6241 ctggatgata ctggcgggga aatagcacat gacggagctg gaaaaacagt tgctgagcgc
     6301 attagagcag ctacagcagg actactcgaa aaggctggac gagtgggaga acgccttcgc
     6361 ggaatggcgg acgatgtctg gtcttatgca acgggagaac gcggcgctga gcgagcgcgt
     6421 cacgggcttg agcaggcagg tgcagagttt gagcgagcag ctgcgccggt tgtcgcaagg
     6481 ctgaatggta tcgaggcgca ccgggagcag gaacgggcgg cgcagcaccg gaaagcgctg
     6541 gagctggaac gatcgcagcg ccagcggacg tacaacggtc cgtcgctgta acaaaccgga
     6601 taaaacgggt ctttcggcgc agatccccgc cgaaaggcgg tgccatcccc ccggctgcat
     6661 ggctatgcat cttggggaaa tttatctggc tcaaaaagcg cggtttagcg gggagtttac
     6721 tgccggtttt accgggctga cgccgcattc gcgccgaacc cgtccggggc ggtgcgggca
     6781 agggatgtta tggtaaatgt ggaatttttt aattatatag agtgtatttt tatgagaatt
     6841 aaaaagcctc accttcgtcc gatagtcata accttgtccg ttggtggtat tttgttgact
     6901 tctatttttc tgattgtcgt tattatggtt tttaaacgcg aaaatataga aaaagacttg
     6961 cttgatgcta attcatcgta cgctatgaaa atgtcagatg taatgagtaa ttatattgaa
     7021 attgctcaac gtgaattggc gtatggcgca aaaaaaatag agtcaactaa tgatattgac
     7081 tcacttaaaa atgaggcgga taggttaaga ttgcagtctg gtatgtttaa ttcagttctt
     7141 gttgttagtg atggtgctgt tgttctggcg acttcgccag aatcacttgg tttagttgga
     7201 gttcacttga attcaagtgc cagtaagttg gcgattgagg ctaaaaaacc atttatatcg
     7261 gcaccttaca aatcagcttc tgggaatttg attatttttt tgtcacatcc tatttatgat
     7321 aagaatagta attatatagg gtatgtcggc gggtctatat atctaaagga acagagttta
     7381 tttagcgacg ttttgagcag gcattttttc aaaagcgaca ctgaaattag cattgttagt
     7441 gatgatggaa atgtgatctt taataacgat aacagtgctg ttggtcgacc aatggttatg
     7501 gatgataatt tgaaatcgaa gttaacttcg tctgagagag gtgagggtat ctttttttct
     7561 tcggggaata aatatcttct tggttatgct catatgaaaa atactgcttg gagtgtgttg
     7621 gtttatagtc acgctgacag cgtgactact attcttatta cttatgctaa gagtgtactt
     7681 gttctgctaa ttgtaatagt tattacattt tcactttttt catatttcat tgcttcccgt
     7741 atttcaattc ctttagaaaa gcttgctata tctaccaatg ctaaagatat agggggttcg
     7801 ctagttgata ttaaaggtat caatgcttgg tatgtcgaag ccgaccgact aaaaaaggca
     7861 ctttttacgc atattaagat aatgatagaa agagttgatt ctctaaatga ggtggcgctt
     7921 aaagatcccc taaccggcgt gaacaacaga ctggggtttt caaataaaat acaaaatcac
     7981 aagcagggtt caggggcttc agttattgct attgatgttg atttttttaa aaaaataaat
     8041 gataacttcg ggcatggggt cggagatgag gttcttgtta ctttggctca ggttattaat
     8101 tgcggttttc ctggcctggg caggttcccc cagtcgcggg ggcagccgga aagaaaacct
     8161 gaatgagctt cagcctcagc gtcaagacgc tgcgccacct ggcataaatg attcctcacc
     8221 gttgaggcat tcaggctgtg gcccaccggc agaatatctt tcagcagacg agtcgtcatt
     8281 tcataggaga tcatcgaggc ccagcgggtt tcaatatatt tcagtgccgg gtgagaataa
     8341 tcgccggccc agtcgctgag caaactgact gtctttgtat cactctcttc acaacggcac
     8401 cggtacaccc gaagcccgga taccggaata acgccaaaca gcgtccggta ccgtattttc
     8461 tgtttgcctt tgattctgcg cgcagcaagg cagtgagggc accggatatg gtgttgagta
     8521 tattcgtctg cctgcagctg gaccaccgac tgctggaccg tattcagcaa cagcttggat
     8581 tctgacaccg ataatccgat gtcgttccgg gtctcccctg atttctggat tgtcatcagt
     8641 tcctccgttc gactggagcc tgattcatcg gtgatgacta tctggagcgt cagttgcatc
     8701 attgcctcct gctgtggtgg atgactatct cccggctact ggttgttcat ctggtgacga
     8761 agcttataat atgtggaaac gccaaggccc gcatggtcgc aggcgatcct gagcctgacc
     8821 cctctgcttc tgagtgcgtc aatttcctgg atctttttga tatcggcgcc ggggcgccct
     8881 gttttttcac cccgtgcttt tttcgcggcg atgccgtcgg cctgcctttc cttcagtaag
     8941 gctcgttcaa actggctgaa tgccgacatc atgtgcagct gcagttcctg catcggattg
     9001 tttgtgccgg cgctgaacgt cagctgctct ttcagaaaaa taagggtaac gccctggtca
     9061 cgaagccggg tggtgacagc gcacatatcc gacatattcc ggcaaaggcg atcgatggaa
     9121 tgaaccagaa gcgtatcacc agggcgtaat tgccccagga gttcctgtaa ccccgggcgg
     9181 tttgtgtcct tcgcgctggc atgctcaaca caaattttat caggctgaaa acccgccttt
     9241 gacaatgcct cttcctgacg ggccgtattc tgatcggccg agcttaccct gatgtaggca
     9301 tattgcacgc aaaacctccg gaaggtcttt aaacatcata gagatttaaa gaataatcca
     9361 taaacactga tttagggagg tttatagaca ttttttcggg gatctgagag gcatgctgaa
     9421 aacgtatacc tttatagaca tcagtcagcg acgacggccc agccagataa cttcaggctc
     9481 cagtaccgga gatgcaaggt cgagcctgct cagttgccgg ttgattttgt ggcggttgaa
     9541 cctgacagca tccaggtcgt cagcaaaagg gcggatctcg ccgaccgttg tttcatcatc
     9601 cgcattaacg atagcttcaa gaaacgccag ggttttatcg aactcatccg cagctgtggc
     9661 tccgggcata ccatggccgc ttatacaaaa cggcgctttc agcgtagagt tttcataaat
     9721 ggcctcaaca cgaatgtcat gaagccagtg ctcaaagaaa ttgtattcat aggtaaagcg
     9781 atcgccagca tcaaaagcaa aatcatcaat cacgacccgg aacggattat cgacaaaacc
     9841 gatgcctcct tcgtaggaga taccgtaatc tttgccataa atgtgaaatt gatggagatg
     9901 gtcgtccccc cagccctgca ctatctggaa aatgaagtga agcgcggcca gtgacgtgtc
     9961 ggcagcaatt ctcagccgac gccagaccat cggactgact ccatgaactg ctattttgat
    10021 gacgtagatc ttcatagtca gcaggta
//
