LOCUS       NZ_CP123926            12163 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Salmonella sp. SA15303 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP123926
VERSION     NZ_CP123926.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33419349
            Assembly: GCF_029962125.1
KEYWORDS    RefSeq.
SOURCE      Salmonella sp. SA15303
  ORGANISM  Salmonella sp. SA15303
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Zhao,w.
  TITLE     Oxford Nanopore Long-Read Sequencing Enables the Generation of
            Complete Bacterial and Plasmid Genomes without Short-Read Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   Zhao,w.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2023) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, Changbai Road, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 11:56:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,944
            Genes (coding)                    :: 4,810
            CDSs (with protein)               :: 4,810
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 38 of 134
            Pseudo Genes (incomplete)         :: 104 of 134
            Pseudo Genes (internal stop)      :: 25 of 134
            Pseudo Genes (multiple problems)  :: 28 of 134
            Pseudo Genes (short protein)      :: 1 of 134
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123926.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Salmonella sp. SA15303"
                     /mol_type="genomic DNA"
                     /strain="SA15303"
                     /host="Homo sapiens"
                     /db_xref="taxon:3030528"
                     /plasmid="unnamed2"
                     /geo_loc_name="China:Beijing"
                     /collection_date="2022-10-28"
                     /collected_by="China Institute of Infectious Disease
                     Control"
     gene            complement(<1..243)
                     /gene="glmM"
                     /locus_tag="QEN31_RS25210"
                     /pseudo=""
     CDS             complement(<1..243)
                     /gene="glmM"
                     /locus_tag="QEN31_RS25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     gene            complement(330..1145)
                     /gene="sul2"
                     /locus_tag="QEN31_RS25215"
     ARG             complement(334..1145)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1235..2325
                     /locus_tag="QEN31_RS25220"
     CDS             join(1235..1775,1775..2325)
                     /locus_tag="QEN31_RS25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612069.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISAba1 family transposase"
                     /protein_id="WP_085940648.1"
                     /translation="MTHLNELYLILNKSLKWNKSHLKCFALIMLVIILKQTCNLSSASK
                     ALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGKRNIN
                     ILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREFIGE
                     QWFTWLIEQDINFCIRVKKNFIVTNHLGKNHKISDLFRHLKVGQIECRKRRILVGRVKL
                     YISALQLENGELLLVVSPQFNANAIQDYALRWEIETLFSCLKGRGFNLENTRLTDPRRV
                     KKLIAVLAISFCWCYLTGEWQHNQKKAIKIKKHGRLSMSLFRYGLDYVQMAIQRLIGFG
                     KKEEFKEILAILRKQNPDRIRVL"
                     /name="IS4-like element ISAba1 family transposase"
     gene            complement(2522..3007)
                     /locus_tag="QEN31_RS25225"
     CDS             complement(2522..3007)
                     /locus_tag="QEN31_RS25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006581703.1"
                     /translation="MQIDIKTSSVKPLRNTYAYIEKRFGDKPASRYQEATYDIQEEINF
                     HYKPLWQPEFDLYDKGRTVIQMKDWYVLKDPRQFYYGAYTQTRAKQQEILESNFTLVEK
                     HDLLRNISEEILNKVTKLLLPLYCKQDIFIFYIQWLIFLLIGNTMKNTMLRKGLTIF"
                     /name="hypothetical protein"
     gene            complement(3233..>4756)
                     /locus_tag="QEN31_RS25230"
                     /pseudo=""
     CDS             complement(3233..>4756)
                     /locus_tag="QEN31_RS25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612398.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            4818..5570
                     /locus_tag="QEN31_RS25235"
     CDS             4818..5570
                     /locus_tag="QEN31_RS25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654541.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_000602738.1"
                     /translation="MILNTIAEKLKRQSKDDFKGRHFEAWLIVQAVAWYLRYPLSYRDL
                     EEMFRERGFEVDHSTINRWVLAYAPVIEKRLRQFRRPHCGSVRIDETYVKIRGKWRYLY
                     RAIDKHGNPVDFLLTAKRDLDAAKRFFRKMLKDEPLLSPNRIGTDGANTFPSTIKTSVD
                     DGLLHPDPVHYVTKHLQQGIESDHFRVKKNMPKIGGFQSFNTARRTIAGFEAMLWLRKG
                     FGFSGGWTVNDQNDLLARLFGLQKVNKA"
                     /name="IS6 family transposase"
     gene            complement(<5681..5986)
                     /locus_tag="QEN31_RS25240"
                     /pseudo=""
     CDS             complement(<5681..5986)
                     /locus_tag="QEN31_RS25240"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /GO_function="GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /name="AAC(3) family N-acetyltransferase"
     gene            complement(5992..7017)
                     /gene="aph(4)-Ia"
                     /locus_tag="QEN31_RS25245"
     ARG             complement(5996..7017)
                     /name="aph(4)-Ia"
                     /gene="aph(4)-Ia"
                     /gene_name="aminoglycoside O-phosphotransferase APH(4)-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7247..8049)
                     /name="AAC(3)-IVa"
                     /gene="AAC(3)-IVa"
                     /gene_name="AAC(3)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.61"
                     /coverage="103.49"
     ARG             complement(7250..8022)
                     /name="aac(3)-IVa"
                     /gene="aac(3)-IVa"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(3)-IVa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8136..8840
                     /locus_tag="QEN31_RS25255"
     CDS             8136..8840
                     /locus_tag="QEN31_RS25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            9029..12036
                     /locus_tag="QEN31_RS25260"
                     /pseudo=""
     CDS             9029..12036
                     /locus_tag="QEN31_RS25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
ORIGIN
        1 tgtcggcagc gggccaaaaa ggaatacgtc catgcccatc gaggtgaaac cggctgtgag
       61 cgcgggttca agcatatagc ccgacaggcg cgtatccttg ccgatcacga cacgatggcg
      121 gtggtcaccg cgacgaaaga cacggccagc cgccatgccg acgcgcaagg cggtttccgc
      181 cgtcatcgcg ccttcgttgg ctttgccacg aataccgtct gtgccgaaat atttgcgcac
      241 cataaggtcg attatcctgt cgtcgggtcg ccctcaaagg ggacatgcct gctgaaccgc
      301 gaatatagag aaatatcccg aatgtgcagt taacgaattc ttgcggtttc tttcagcgcc
      361 gccaataccg ccagcccgtc gcgcaagggg cgcggctcgt gtgtgcggat gaagtcagct
      421 ccacctgcgg cggcggcaag ctctgcagcg agtgtcgcgg ccccgacatc ccccggacca
      481 cggcctgtga gcgcgcgcag aaaggatttg cgcgaaacag acagaagcac cggcaaatcg
      541 aagcgcagcc gcaattcatc gaaccgcgcc agcaccgaga gcgaggtttc gggagcagcc
      601 cccagaaaaa accccatgcc gggatcaagg acaaggcggt tgcgtttgat accggcaccc
      661 gtcagcgccg cgatgcgcgc gtcaaagaac gccgcaatgt gatccatgat gtcgccagcg
      721 ggtgcctcgc gccgatctgc ctgcccgtct tgcaccgaat gcataacgac gagtttggca
      781 gatgatttcg ccaattgcgg atagaacgca gcgtctggaa aaccgcgaat atcattgaga
      841 taggccacac cacgcgacaa ggcataggct tgcgtcgcgg gttgataact gtcgagcgag
      901 acgggaatgc catctgcctt gagcgcgtcc agcaccggcg cgatacgcgc gatttctgtg
      961 tcggacgaaa caggcgcggc gtcgggattg ctggatgccg gaccgaggtc gatcacatct
     1021 gccccctcgg ccatcagctt acgcgcctgc gcaatggctg cgtctggcgc cagataccgg
     1081 cctccatcgg agaaactgtc cgaggttatg ttgacgatgc cgaaaatgat gagcgattta
     1141 ttcatggggg cttctataat aatctctgta cacgacaaaa atagataact cattgaaata
     1201 atgtcacaat aattgttttc taacgacgaa tactatgaca catctcaatg agttatatct
     1261 tatcttaaac aaatctctaa aatggaacaa gtcacattta aagtgctttg cgctcatcat
     1321 gcttgtgatt attttaaagc aaacatgtaa tctttcttct gcatctaaag ccttgcccat
     1381 caagtgttta ccacaatcat tttatcgacg tatgcagcgc ttctttgcag gtcagtattt
     1441 tgattatcgt caaatttctc agttgatttt caatatgttt tcattcgacc aagtgcaact
     1501 gactttagat agaaccaatt ggaaatgggg aaaacgaaat attaatatcc tgatgctcgc
     1561 aatcgtttat cgtggaatag cgatacctat cctttggaca ttgcttaata aacgtggaaa
     1621 ttcagatacg aaagagcgta ttgctttgat tcaacgcttt atagccattt ttggtaaaga
     1681 ccgtattgtg aatgtgttcg cagacagaga gtttatcggt gagcagtggt ttacatggtt
     1741 aattgaacaa gacatcaact tctgcattcg tgttaaaaaa acttcattgt caccaatcat
     1801 ttaggaaaga atcataaaat tagtgattta tttcgccatc ttaaagttgg tcaaattgaa
     1861 tgtcgtaaac gacggatttt ggttggtcgg gtgaaactat atataagtgc actacagtta
     1921 gaaaatggag agcttttact cgtcgtttct cctcagttta atgccaatgc tattcaggat
     1981 tatgcattac gctgggaaat tgaaacctta ttcagttgtc tcaaaggacg cgggtttaat
     2041 cttgaaaata cgcgcttgac agaccctaga cgagtgaaaa aattgattgc ggtgttagct
     2101 ataagcttct gttggtgtta cttaacgggt gaatggcaac ataatcaaaa aaaagcgata
     2161 aaaataaaga agcatggacg actctcaatg agtttatttc gctatggttt agactatgtt
     2221 caaatggcga ttcagcgttt aattggtttt gggaaaaaag aagagtttaa ggaaattttg
     2281 gcaattttaa gaaagcagaa tcctgatagg ataagggttc tgtgaaattt gtcgtgtaca
     2341 gagtataata ataataatcg agcatgagtc tcatacggat gctcgggtcg aaagggaatc
     2401 cccaggcgag taacctgttt gcggtgatcc attagctgca ggagcagaag agcatacatc
     2461 tggaagcaaa gccaggaaag cggcctatgg agctgtgcgg cagcgctcag taggcaattt
     2521 ttcaaaatat tgttaagcct tttctgagca tggtattttt catggtatta ccaattagca
     2581 ggaaaataag ccattgaata taaaagataa aaatgtcttg tttacaataa agtgggagta
     2641 gtaatttcgt tactttgttt agaatttctt cagaaatatt tcttaataaa tcgtgttttt
     2701 caacaagcgt aaaattactt tctaaaatct cttgttgttt agctcgagtt tgggtataag
     2761 ccccataata aaattgacga ggatctttaa gcacgtacca atctttcatt tggattacag
     2821 tacggccctt atcgtaaaga tcaaattcag gctgccataa aggtttgtaa tgaaaattaa
     2881 tttcttcctg aatatcgtag gtcgcttctt gataacgtga agctggttta tcaccaaatc
     2941 ttttttcaat ataagcataa gtatttctta aaggcttaac tgaagatgtt ttaatatcaa
     3001 tttgcatata aatcttcctg attttttaat aaagactaga gtttttattc tctaataccg
     3061 gcctgtccaa atcgccactt catgcggtca tattcaatct ttgcaaaatc atccgtactt
     3121 aggtgctgta cttgatggtg tgcgcagaat tcttcaaaag ccttgactgg cataatcatt
     3181 tctacagcta actctggatg gggtcgtttg cgggaggggg cggaatccta cgctaaggct
     3241 ttggccagcg atattctccg gtgagattga tgtgttccca tccgagcggc gaaacatggg
     3301 ccaagagatc gggcgatagc agctttccat cgcgtttctg gtttgcaacg acctcgccga
     3361 gcttcatggt gttccagaag atgatgatgg cggcgagcag attcatgccg gcgatgcggt
     3421 aatgctggcc ttcggcggaa cggtcgcgga tttcaccgcg gcggtggaag ctgattgccc
     3481 gcttcagcgc atgatgagct tcgcctttgt tgagcccgat ctgggcacgc cgttggagtt
     3541 cggcatccag aatccagtcg atcatgaaca gggtgcgctc gacgcgaccg acttcccgca
     3601 gggctgtcgc gagctcgttc tgccgcggat aggaggcgag tttccgcaga atctggcttg
     3661 gcgcgacggt cccggcagca atggtggcgg cgatgcgcag gatgtcgggc caattgcgct
     3721 cgatcatggc ttggttgacc tttccgccga tcaacgctcg caggtgcgcc ggggcggccg
     3781 acggattgaa cgcgtagagc cgtttggatg gcaggtcgcg gatgcgcgga gcgaaccggt
     3841 agccgagaat ggcacatgcg gcaaagacgt gatcggtgaa gccgcccgtg tcggtgaact
     3901 gctcgcggat atggcgtcca gcatcgttca tcagcaggcc atcgaggatg taaggcgctt
     3961 cgcttgccgt tgcaggaatc acctgggttg cgaacggcgc atattggtcg gagacgtggc
     4021 tataggcttt caggcccggg gtattgccat atttcgcgtt gaccaggttc atggcctcac
     4081 cttgctctgt agcgacgaag aactgtccgt cgctcgaagc cgacgtgccc atgccccaga
     4141 accgggccat gggtaacgct gcctgtgcct cgaccaccat ggccagcgcc cggtcatagg
     4201 cttcgccctc gacatgccac cgtccaatgc ggatcaattc ccagaaggtg tgggtgtttg
     4261 tcgcatccgc cattttgcgc aagccgaggt tgatcccttc cgccaagata acgttcatta
     4321 gcccgatccg gtcagcgcag ggtgctcctg tgcgcagatg ggtgaacgct tcggtgaagc
     4381 cggtcgccgc atccacctcc agcaggagat cggtgatgcg cgtgggcggg atctgcttgt
     4441 agagatcgag caccagatct tcggcgcctg tcggcgcggc ggcttcgagt ttctcgatat
     4501 gcagaacgcc gttttcaatc gacccgcccg ggatcgtgcc tgcgcgagcg gcacggccaa
     4561 gctcgcgcaa ccgcatgtcg aggcgagctt gccggtctgc cagccattcc tccggccgca
     4621 atggcacagc gagacgaccg ccttccgcga tggattgtgc cggaacgagt gcgtgtttca
     4681 gatcgccata gcgccgggac ctagtaagcc agacatctcc ggagcggaac gcatggcctt
     4741 gtcgcaaatt cttcaggtta actcgatgtt gaccatgggg agagaagttg ccgctcctga
     4801 tattgcggag cgagccgatg attttgaata ccattgccga aaagctgaag cgccagtcga
     4861 aggatgattt caagggcagg catttcgagg cctggctgat tgtacaggcg gttgcctggt
     4921 acttgcgcta cccgctcagc tatcgtgact tggaagagat gtttcgcgag cggggcttcg
     4981 aggtcgatca tagcacgatc aaccgctggg tattggctta tgcacctgtc atcgagaaac
     5041 ggctgcggca gtttcgtcga ccccattgcg gctcggtccg gattgatgag acctatgtca
     5101 agatccgcgg caaatggcgc tatctgtacc gagccatcga taagcatggc aatccggtgg
     5161 atttcctgct gaccgctaag cgcgatctcg acgctgccaa gcggttcttc cgaaagatgc
     5221 ttaaagatga acccttgttg tcgccgaaca ggattgggac ggacggggcc aacaccttcc
     5281 cgtcaacgat caaaacgtcg gttgatgatg ggcttctcca tcccgatccc gtgcattatg
     5341 tgaccaaaca tctccagcag gggattgaga gcgaccattt ccgggtgaag aagaacatgc
     5401 cgaagatcgg cggtttccaa tcctttaaca cggcgcggcg aaccatcgcg ggtttcgagg
     5461 cgatgctgtg gctgcggaag ggctttggct tttcaggcgg ctggaccgtc aatgaccaga
     5521 atgatttgct tgcgcgcctc ttcggactgc aaaaggttaa caaagcatga aaataccggc
     5581 cttggctggg tgatagctgc ctccaaaagt gtttgcgaca cgcccggaac aattcagcca
     5641 ctgcacccat gttccgagtt ggtgtggttt gcggatcgta tgccggcatc tcgttgcgca
     5701 agatctgcac ccaatcggca ggcacgggcg gcgatctcca atctgcggga tcagtcagat
     5761 cacccgagtg cgtgggcatg acaatcgtgc cctggggacc aacacaatcc agaagggcct
     5821 gaatcactgc gaccggccct cccgcgaccc agccgagcga gcttagcgaa ctgtggacga
     5881 gaactgtgcc accaagcgta aggccgttct ctcgcatttg ccttgctagg ctcgcgcgag
     5941 ttgctggctg aggcgttctc gaaatcagct cttgttcggt cggcatctac tctattcctt
     6001 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     6061 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     6121 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     6181 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     6241 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     6301 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     6361 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     6421 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     6481 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     6541 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     6601 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     6661 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     6721 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     6781 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     6841 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     6901 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     6961 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
     7021 tcattgcccc cggacgagcg tctgctccgc cattcgccgt ccgccgtgcc aatcggatca
     7081 gccgtccaaa tgcgggattt tcgttagtcg gaggccaaac ggcattgagc gtcagcatat
     7141 catcagcgag ctgaagaaag acaatccccg atccgctcca cgtgttgccc cagcaatcag
     7201 cgcgaccttg cccctccaac gtcatctcgt tctccgctca tgagctcagc caatcgactg
     7261 gcgagcggca tcgcattctt cgcatcccgc ctctggcgga tgcaggaaga tcaacggatc
     7321 tcggcccagt tgacccaggg ctgtcgccac aatgtcgcgg gagcggatca accgagcaaa
     7381 ggcatgaccg actggacctt ccttctgaag gctcttctcc ttgagccacc tgtccgccaa
     7441 ggcaaagcgc tcacagcagt ggtcattctc gagataatcg acgcgtacca acttgccatc
     7501 ctgaagaatg gtgcagtgtc tcggcacccc atagggaacc tttgccatca actcggcaag
     7561 atgcagcgtc gtgttggcat cgtgtcccac gccgaggaga agtacctgcc catcgagttc
     7621 atggacacgg gcgaccgggc ttgcaggcga gtgaggtggc aggggcaatg gatcagagat
     7681 gatctgctct gcctgtggcc ccgctgccgc aaaggcaaat ggatgggcgc tgcgctttac
     7741 atttggcagg cgccagaatg tgtcagagac aactccaagg tccggtgtaa cgggcgacgt
     7801 ggcaggatcg aacggctcgt cgtccagacc tgaccacgag ggcatgacga gcgtccctcc
     7861 cggacccagc gcagcacgca gggcctcgat cagtccaagt ggcccatctt cgaggggccg
     7921 gacgctacgg aaggagctgt ggaccagcag cacaccgccg ggggtaaccc caaggttgag
     7981 aagctgaccg atgagctcgg cttttcgcca ttcgtattgc acgacattgc actccaccgc
     8041 tgatgacatc agtcgatcat agcacgatca acggcactgt tgcaaatagt cggtggtgat
     8101 aaacttatca tccccttttg ctgatggagc tgcacatgaa cccattcaaa ggccggcatt
     8161 ttcagcgtga catcattctg tgggccgtac gctggtactg caaatacggc atcagttacc
     8221 gtgagctgca ggagatgctg gctgaacgcg gagtgaatgt cgatcactcc acgatttacc
     8281 gctgggttca gcgttatgcg cctgaaatgg aaaaacggct gcgctggtac tggcgtaacc
     8341 cttccgatct ttgcccgtgg cacatggatg aaacctacgt gaaggtcaat ggccgctggg
     8401 cgtatctgta ccgggccgtc gacagccggg gccgcactgt cgatttttat ctctcctccc
     8461 gtcgtaacag caaagctgca taccggtttc tgggtaaaat cctcaacaac gtgaagaagt
     8521 ggcagatccc gcgattcatc aacacggata aagcgcccgc ctatggtcgc gcgcttgctc
     8581 tgctcaaacg cgaaggccgg tgcccgtctg acgttgaaca ccgacagatt aagtaccgga
     8641 acaacgtgat tgaatgcgat catggcaaac tgaaacggat aatcggcgcc acgctgggat
     8701 ttaaatccat gaagacggct tacgccacca tcaaaggtat tgaggtgatg cgtgcactac
     8761 gcaaaggcca ggcctcagca ttttattatg gtgatcccct gggcgaaatg cgcctggtaa
     8821 gcagagtttt tgaaatgtaa ggcctttgaa taagacaaaa ggctgcctca tcgctaactt
     8881 tgcaacagtg ccatactgtg tagtatcata atgcataaat cacgattttt gatactgaca
     8941 ttcagtaccg ttttgggtgg tatcaaaact cataacattt gagacggtta aaaagagaac
     9001 agaacacccg tttacgaaag gatgcgtgat ggctgctgat tttctgaccg acaaacagac
     9061 acagaactac gggcgttatg ccgcagagcc aaacgagatc cagctggcac ggtattttca
     9121 tcttgatgag cgagacctta ctttcatcaa cctgcgtagg ggcaggcata accggttggg
     9181 aattgccctt caactcacaa ctgcccgctt tctgggaacc ttcttgtccg atctcatgca
     9241 aataccgccc ggtgtccagt tttatgtcgc aaggcaactt aacatccgct atccagagat
     9301 catttcccgc tatgctcaaa gggaaaacac acgttgggag caccacgggc ttatcaggca
     9361 gcactatagc tatcatgatt tcggtgattt cccgtggtcg ttcagactga agcgattgct
     9421 gtatacccgt gcatggctca gtaatgaacg ccccggactg atgttcgatt ttgccaccgc
     9481 gtggttgctt caaaataaag tgttgttgcc agccgcatcc accctgacga gagtcattgg
     9541 tgaaatccgt gagcgtgcga cccgccgctt gtggcgaaaa ttggccgcgc tgccaaaccg
     9601 ttggcagacc gcacaactgg ctgggttact tgaaatcccc gaaggacagc gactctcagt
     9661 gatggagcac ctaaaaagag gccctgtcac tatcagcggc cccgcgttca ctgaagcact
     9721 tgaacgttac actcgcctgc gcagcctgga gttttcctgt ctgaatttca ctgggctgcc
     9781 cgccatacag ctccgcaatc tggcacgcta tgccggaatg gcatcggtga aatatatcag
     9841 cagaatgcca gaagaacggc ggctggcgat ccttaccgca ttcgtgaaag cgcaagaaat
     9901 ctcggcgctg gacgaggccg ttgacgtgct ggatatgctg atcctcaata ttacccggga
     9961 ggcgaagaaa accgggcaga aaaagcgact taggacgctc aaggatcttg accgcgcggc
    10021 gttgctgctg gcgcgggcat gcgcactttt acttgatgaa gataccgccg atgatttgct
    10081 gcgaaagacc atattcagca gtgtgtctgt cgccagactg gcagagtccg tggaaaaagt
    10141 gaatgaactg gcccgccccc aggatacaaa ctttcaggat gaaatggtgg agcaatacgg
    10201 acgggtaagg cgttttttgc ccgcactact gcgcgatctg cacttccgtg cggccccaga
    10261 cggtgaacat accttggctg ccatccatta cctggcagag ctaaacggct cgaaaaaacg
    10321 gatcctggac gatgcacctg aacatatcat ttcaggccct tggaaacgcc tggtgtacga
    10381 tgctgacggt cggatacagc gagcaggtta ctcgttgtgt ctgctggagc gacttcagga
    10441 tgcgctacgc cgtcgtgaca tctggttgga aaacagcgat cgttggggtg atccgcgcca
    10501 aaaacttctt cagggggagg aatggcaggc gcagcgggtt ccagtctgcc gggcattggg
    10561 tcaccccact aatggcagta aagcatcgga gcaattggcc gctcagttag atgaaacctg
    10621 gaaaacggtg gcatcacgtt ttgatcgcaa taccgccgtg gacatctgca atgaaggtaa
    10681 acatccttcg ctgaccatca gcagcctgga taaattggat gaaccaccgg cgctgataca
    10741 actgagcagc cgagtcagac aattgcttcc acccgttgac ctgacagaac tgttgctcga
    10801 gattgacgcc agaactggct tcacccgtga attcagccat gtcagcgagt caggtgcccg
    10861 tgcgcaggat ctgcatataa gcctgtgtgc ggtgatgctg gctgaggcct gcaatatcgg
    10921 acatgagccg ctgatcaagc acaatatccc tgcactgact cgccatcgtc tgagctgggt
    10981 gaaacagaac tacatccgag cagaaacgct ggttagcgcc aatgcgcggc tggttgattt
    11041 tcagtcctcg ttagcgttgg caggatactg gggggcgggg aggtagcctc cgctgatggt
    11101 atgcgttttg tcacgccagt gaaaacggtg aattctggcc ccaacagaaa atactttggt
    11161 tcagggcgcg gcatcacctg gtacaacttt gtctctgacc aatattcggg ttttcatggc
    11221 atagtgattc ctggaacact gcgggattcc atctttgtgc tggaagggct acttgagcaa
    11281 caaacgggac taaacccggt tgaaatcatg acggacacag caggtaccag tgacattatc
    11341 tttggcctgt tctggctact gggataccaa ttttcacctc gtctggcgga tgcgggtgag
    11401 gctgtgttct ggcgggccga caaagcagcg aattacgggg cgttggacaa actggcgcgg
    11461 ggttgtgttg acctttctaa aatcgaaagc cactgggatg agatgatgcg ggttgccggt
    11521 tcgctgaagc tgggcactat tcatgcgtca gaactcattc gatcgctatt gagaagtacc
    11581 cgaccgtcag ggcttgctca ggcaataatg gaggtcggaa gagttaacaa gacactgtat
    11641 cttctcaact atattgatga tgaggactat cgtcgccgga tcctaacaca gttgaaccgg
    11701 ggtgaaggcc gccatgccgt tgcaagagcc atctgctacg gtcagcgtgg cgagatcaga
    11761 aaacgttacc gcgaggggca ggaagatcag ttgggtgcgc tgggtctggt gacaaacgca
    11821 gtggtgctgt ggaatacgct ttatatgcag gaagccttgt cgcacctgcg cagcatcggg
    11881 gaagggccag aggatgaaca tatcgcgcgg ctttcgcctc tgatgcacgg tcatatcaac
    11941 atgctgggac attatacgtt cacactaccg gaggatatta tgaaggggga attgaggccg
    12001 ttaaatctca atttaaacaa tgaattatct ccttagcgtg gttttttaca tcattggccc
    12061 tcaaacccca tgatgatggt ttcgaaacgt cttaaggcca aagactatcg aacggtttcc
    12121 acgggattta acgtcctgag ctctaagcca gggctgataa ctt
//
