LOCUS       NZ_CP123921             6078 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Acinetobacter bereziniae strain UCO-554 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP123921
VERSION     NZ_CP123921.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34266404
            Assembly: GCF_029916905.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter bereziniae
  ORGANISM  Acinetobacter bereziniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Opazo-Capurro,A., Xanthopoulou,K., Gonzalez-Munoz,P.,
            Hormazabal,J.C., Vera,R., Higgins,P.G. and Gonzalez-Rocha,G.
  TITLE     Carbapenem resistance in Acinetobacter bereziniae from Chile is
            mediated by plasmid-encoded NDM-1 carbapenemases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Opazo-Capurro,A., Xanthopoulou,K., Gonzalez-Munoz,P.,
            Hormazabal,J.C., Vera,R., Higgins,P.G. and Gonzalez-Rocha,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2023) Microbiologia, Universidad de Concepcion,
            Barrio Universitario S/N, Concepcion, Bio-Bio 4070386, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 10:34:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,328
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 4,156
            CDSs (with protein)               :: 4,156
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 49 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 16 of 69
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123921.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter bereziniae"
                     /mol_type="genomic DNA"
                     /strain="UCO-554"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:106648"
                     /plasmid="unnamed3"
                     /geo_loc_name="Chile: Santiago"
                     /lat_lon="33.26 S 70.38 W"
                     /collection_date="2020-10-16"
                     /collected_by="Hospital de Urgencia Asistencia Pblica"
     gene            join(5761..6078,1..351)
                     /locus_tag="QHG51_RS21605"
                     /old_locus_tag="QHG51_21600"
     CDS             join(5761..6078,1..351)
                     /locus_tag="QHG51_RS21605"
                     /old_locus_tag="QHG51_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(510..1043)
                     /gene="aadB"
                     /locus_tag="QHG51_RS21610"
                     /old_locus_tag="QHG51_21605"
     CDS             complement(510..1043)
                     /gene="aadB"
                     /locus_tag="QHG51_RS21610"
                     /old_locus_tag="QHG51_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(1134..1820)
                     /locus_tag="QHG51_RS21615"
                     /old_locus_tag="QHG51_21610"
     CDS             complement(1134..1820)
                     /locus_tag="QHG51_RS21615"
                     /old_locus_tag="QHG51_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            2823..3230
                     /locus_tag="QHG51_RS21620"
                     /old_locus_tag="QHG51_21615"
     CDS             2823..3230
                     /locus_tag="QHG51_RS21620"
                     /old_locus_tag="QHG51_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_281104429.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSSNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3220..5034
                     /locus_tag="QHG51_RS21625"
                     /old_locus_tag="QHG51_21620"
     CDS             3220..5034
                     /locus_tag="QHG51_RS21625"
                     /old_locus_tag="QHG51_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5143..5628
                     /locus_tag="QHG51_RS21630"
                     /old_locus_tag="QHG51_21625"
     CDS             5143..5628
                     /locus_tag="QHG51_RS21630"
                     /old_locus_tag="QHG51_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     ARG             complement(514..1043)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(511..1055)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          3221..5031
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatagtgcta aagagaagtt cccagattcg cctagtaaaa tgatccctga gcttaaattt
       61 gtattctggc aaaagatgct tatggctaat tatgaagaac gtatttggca gggatgtttt
      121 aattctattt ttcctaatgc aacagttgaa aacaggcaaa ttttttacga ttggacagat
      181 caattacgtg aattaagaaa caggattgct catcatgaac cgattatttt taaccgtaat
      241 ctagaaaatg atttagaggt tttaacaaaa tttatttact gtcgttgtat taatacatac
      301 gattttatcg agcaatctgc attagatcta aaagaatatt tagattttta agataagaat
      361 ttcaccccaa acaatcgaaa ggtcagacta ttttgcaaat gttgaattaa caaggttagt
      421 agtaaagcag taaatccgat aacggtagca gtgtttccta acacctgagt taagccgcgc
      481 cgcaaagcgg cgtcggcttg gacgaattgt taggccgcat atcgcgacct gaaagcggca
      541 cgcaagacct caaccttttc cgccccgagt gaggtgcatg cgaacctgta ggactctatg
      601 tgctttgtag gccagtccac tggtggtact tcatcggcat agtaaaagta atcccagatg
      661 atcgcctccc agctgttaca acggactggc cgcccggcga tgacaccctc agccgcctct
      721 gggcacgagc cctgcggagc ctccgcgatt tcatacgctt cgtctgccca ccaagcaggt
      781 tcgcagtcaa gtaactcatc cccgatctcc gctaagaatc catagtccaa ctcctccgtg
      841 acgcgcccgc cgagcatttc aactattgcc tcgagctcgc cgcgcctctc gccgggaaaa
      901 gtcagatcaa tatcatcgtg cttgcgtgtt acacgcccta gccgtgcatc gatcgcccag
      961 cccccaccga tccagagcgg cagatttcgc tcatctgccg cagctagaat ttggtgtatc
     1021 aatgcgacct gcgttgtgtc catgcggcct aacatagtaa gccctcccag aatgtcagta
     1081 attagactat taattgaatt gttacatcca atagtgagct cccagtgatt gtcttaacta
     1141 tccaaccttt cgattggatg gggcagagca ccattattct gtttgaatgc ggtgttagtg
     1201 tcaactaggt tctttatttc ttcaaggagt ttgaaagtgc taagtatttt tactttactt
     1261 tgttcctcaa tataaatttt tttaagacca tttagcagct tgtatttgga tattttggat
     1321 aggagaatga ataaggttgc ggtattgatt cttccccctt ttagatgaag taaacggttt
     1381 ttttgcagta ttggaatatt tctatattct gttagttgat cttttaagtc atcaggaatt
     1441 ttcatttcgt aaaaattata ttttttagaa cagtctaata tcgagtgatt taggctttct
     1501 gtatttgtat taataaaaat gcatttttca aatgtgcaat ttttaaagcg acaatctcta
     1561 aaaatagcag cataaaatac gcactttttg aagtgaacat tcgtaaacct attcttattt
     1621 aagattactc ctgaaaaatc agataaaaca atttttatat ttttgaatga acatgatcgt
     1681 aggcgggatc ctctcaaatt aacattaaaa aatgttttat tagagaaacg tactttagaa
     1741 gaaatagatt tggctaggag gctatttgaa tacttacgtt tttcgatgtg cttgttatga
     1801 aatctaggaa gttttgacat taatagtcca aagaatagga gtttcagctt aattcttagg
     1861 tgtaatcaaa aaaaataatg gacaggtctc ttgcgaaatc tgtccattag ttttaaattc
     1921 gatgggtaca gttatttgat gcttgcaaca tcaattaacg aaagatcggc agagcttgca
     1981 actcatactg atattcacct taaaggctag taccactatc tattttaaag aattagaaca
     2041 gtttgacaag cctcaatttg aagtccttcg ttaattcagc agtttaaatc atcaaataag
     2101 cacctaacgg tcgagcttta tctacttctg tgcgcctttt ttaaggaaga tatgcgttgc
     2161 atgtggaggg aataagtgtc actggtgcat cgtagcgata gtcgcaaggc taaaccgctt
     2221 acaggggatc tcacataggc ggagcgttgg aagtagttgt ataggttggt taagttgcat
     2281 gttggagtaa tcctcgcaga gataccacca tacttcgaca gggagcgcgt gggagaaggt
     2341 gcaaaacctt tagtccttgt ctgcatggtc agcaaaacca tgtgcggata cgaccgatgc
     2401 gtggctccgt cagcccaatt acaggttaat cggcaatact taagaggata tttgattttt
     2461 ttaaatcaaa gcctcttagg gaaagtattg ccgaaactct ctcaacgttc accatcagcc
     2521 cgagcaaagc gagggatgtg gggacggtga gaaagagttg agcgcatttg tttttacaag
     2581 attcatgcca atacctacta gagtttatat accgtgtcat ttgattagtt tttattgaat
     2641 ttcactcgtc catttttaaa taagcaagga tacacagcta cccctattta acttcgttaa
     2701 acagtgatag tctgtgacct tgatcagggg cttcaccccc gatacccccg aaaagcttta
     2761 aacagaaaaa tcaaagattt tccaatttaa agcttacaaa agccaaagca gggtagattg
     2821 aaatgtcagg acaggaacaa gaaaacaaac agagcaaagc agttcgagaa cgaaccttta
     2881 aagtcagact ttcagaatca gaatatcaat tgctcgattc cagtaatcct tatggttcga
     2941 ttgcaaagtt gcttagacaa gcagcagtag agaaagcgac taaaattcat aattccaaag
     3001 cagaagatga ccaaaaaatt aaagcaacaa aggttgttga atcttcgtat agcaagacag
     3061 agcgagcatt aattttagag cttggtcgga ttggcaacaa tgtgaatcag attgctaaag
     3121 cagtgaatac cgatattgca ggggtaggta cttttgacaa agtgaagtta cttcatttgt
     3181 tgatttccat agatcagcag cttcgggagt tacgtccaga tgatcgttga tttctttaga
     3241 catggttcag gtttatcaaa aggctgtctc gattatttgt taggtgaaga tcgagagcgt
     3301 gaacatgctc aagtgctcaa tggtgatgta gaactgacag cacaactcat tgatagcagt
     3361 ccttttgcca aaaaatatac cagtggttgt ttgtcctttt atgagcatga tttatctgac
     3421 caggataaac agaaaatcat gcaaaatttt gaggaatgtt tattcccggg actagataaa
     3481 gaccaatatc aaattttatg ggtacagcac caagacaaaa ttaatcagga cacaggtgag
     3541 acaaggcttg agcttaattt tgtgattcct aatgttgagc tttcaacagg caaacgccta
     3601 cagccattct atgcgccagt cgatttagat cgagtcgatc tatttaagca gatcactaat
     3661 accgaacatt cactgtatga ccccgatgat cctgagcatc gacagctatt tttaaataaa
     3721 aagaacctac ccaaagatat taaggatttt aaagcgcagt tacatcaacg tgtttaccga
     3781 gcggttgcca atggtgatgt tgccgatcgg caagaattgg tgcaatggct tgaatcgaat
     3841 caaatcaatg tcactcgaca agtaaaaaac tccatatcca ttgaaaaccc atatgagggc
     3901 gcaaaacgtc ccatccgttt agagggcgag atttatgagc aaggctttag agctactggc
     3961 gaatatagac aagaagtaca acaacgaatt gaagaataca gaggaacaac ttctgagcgg
     4021 tatcgaacaa acgtcacaga ctatcagaga cagcttgaac ataagagcca atatcactgc
     4081 gaccgctatc caacagtcgg acgagaaaat agcccagagc attcaaaaca acgcccagac
     4141 ggtcgagaag cagttaagcc tgtatcaaac cttgctgcaa tcgaaattga gccatttaac
     4201 gcaattaaac gagcaaatac agagccaaga acaacaagcc cagaatcaag cggtacagaa
     4261 aaaacttacc actttgaata tgggactgat ttcagtagca gttattttgc ttatagcaat
     4321 tttctcgctt ggtctcgtca ccaaaaacaa gtacagcgag atgcagacgc taaataccgt
     4381 gatcaacgag aagcaaatga gggtcgacca tttgaaccga tcgggaggca gtttgacgat
     4441 cagcacatgc aagcgaccag acagcaaagc acagcgccta tgtattcaga tcaacaagaa
     4501 cgcaggcgaa tggcagaacg gctacatgat tccaatgggg tattaaacga tgaccgaatt
     4561 agaaacacaa ttattgagaa tcatcgaaga acaacagcaa caattaatgc aacaacaagt
     4621 gcaattgcag aagcaacagc aagctttaga cacaatgcaa accaaaatta tccaagcctt
     4681 gtcagcacaa tcaaagacaa tcaagagcga tcagcaaagc ttacagcaag cacagaaatc
     4741 attagcgaca atgcagaagc aatttcacga gctagaacga agtacagcaa gctataccga
     4801 gcagatcaat ggcaaagtcc gagagatgag ccttatcaca gccaacaacg aagaaattct
     4861 ggagagaatc tcaaccacac agcattctgt ctcgtcagaa gtagccaaag cgcagcagaa
     4921 tttgctcgaa acatccgaga tattgaaaaa aatctcgtcc agatgaatga gcgtaaacaa
     4981 gagatagaaa aacctcgacc taagcaagat cgaggtatgg attttgggat gtaattattt
     5041 tctggtatgt tggtgtttat ttcttagtat tagcttgtac taatgtttaa atttagctat
     5101 attgaatgta gtataggctt gtttatatat tttggtggtg ttatgaataa taactgtgta
     5161 gtagtactaa ctgctcgtgg aaagactaga attttaaaag aaggtggtag tcagtcttgg
     5221 agactaaatg ctcataatgc aggaaagtgc gattatctag tttgtgtgca aaataggaaa
     5281 caatcttggg gagaaccaga ggctgatcat cacactgcat ttttagttgg caaaatatct
     5341 agtatcagcc aatctcaaga agaagatgca gatggtagat ggataataaa tatctccgaa
     5401 tatgctgata tatctattcc caatatgtgg gatggtaatc ggaatccagt ttcttatagt
     5461 tcattagaag agatgaatat cgatttgaat gagctgaaat ttaccaaatt aaatgaagat
     5521 aattcttcta aaacactgga taaaatatct attcctgaag ctaagagact attagctaat
     5581 tattttgatg tccctcaaga aaatatttca attacaatca atatgtaagc ttttaattga
     5641 ccaaaaaaag aaaaaagcct ttcacgttaa tgaaaggctt ttttgaatct tagcgcttgg
     5701 gagggaagat cttagatccg cagtctctca aacttacaac tatatattag ggtatatacg
     5761 gtggaattgt caaatgatgc agtgcttaag atagagcagg ctttatctat accacgctta
     5821 tctaagtatg aaaatttcta taaagataaa ggtgaaccat atgaaaaatc tgatgtattg
     5881 atgctttatg aacgtaattt aataatttcc aataagtttt tttacttatt aaattatttt
     5941 gaagttgttc tgagaaatgc tgttgttcag gcaatagaaa tatcttttag gtgtaatgaa
     6001 acaaatagtt ggcatgaaaa tgaagctttt attcgaagcc ttagccgtag agggaggtat
     6061 agccctaaaa gtatgttt
//
