LOCUS       NZ_CP123916             6078 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Acinetobacter bereziniae strain UCO-553 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP123916
VERSION     NZ_CP123916.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34266403
            Assembly: GCF_029916945.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter bereziniae
  ORGANISM  Acinetobacter bereziniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Opazo-Capurro,A., Xanthopoulou,K., Gonzalez-Munoz,P.,
            Hormazabal,J.C., Vera,R., Higgins,P.G. and Gonzalez-Rocha,G.
  TITLE     Carbapenem resistance in Acinetobacter bereziniae from Chile is
            mediated by plasmid-encoded NDM-1 carbapenemases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Opazo-Capurro,A., Xanthopoulou,K., Gonzalez-Munoz,P.,
            Hormazabal,J.C., Vera,R., Higgins,P.G. and Gonzalez-Rocha,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2023) Microbiologia, Universidad de Concepcion,
            Barrio Universitario S/N, Concepcion, Bio-Bio 4070386, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029916945.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 05:33:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,329
            CDSs (total)                      :: 4,226
            Genes (coding)                    :: 4,159
            CDSs (with protein)               :: 4,159
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 25 of 67
            Pseudo Genes (incomplete)         :: 47 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123916.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter bereziniae"
                     /mol_type="genomic DNA"
                     /strain="UCO-553"
                     /isolation_source="tracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:106648"
                     /plasmid="unnamed4"
                     /geo_loc_name="Chile: Santiago"
                     /lat_lon="33.26 S 70.38 W"
                     /collection_date="2021-02-16"
                     /collected_by="Hospital de Urgencia Asistencia Pblica"
     gene            634..1041
                     /locus_tag="QHG52_RS21610"
     CDS             634..1041
                     /locus_tag="QHG52_RS21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_281104429.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSSNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1031..2845
                     /locus_tag="QHG52_RS21615"
     CDS             1031..2845
                     /locus_tag="QHG52_RS21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2954..3439
                     /locus_tag="QHG52_RS21620"
     CDS             2954..3439
                     /locus_tag="QHG52_RS21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            3572..4240
                     /locus_tag="QHG52_RS21625"
     CDS             3572..4240
                     /locus_tag="QHG52_RS21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(4399..4932)
                     /gene="aadB"
                     /locus_tag="QHG52_RS21630"
     CDS             complement(4399..4932)
                     /gene="aadB"
                     /locus_tag="QHG52_RS21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(5023..5709)
                     /locus_tag="QHG52_RS21635"
     CDS             complement(5023..5709)
                     /locus_tag="QHG52_RS21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     ARG             complement(4403..4932)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(4400..4944)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          1032..2842
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgtagcgat agtcgcaagg ctaaaccgct tacaggggat ctcacatagg cggagcgttg
       61 gaagtagttg tataggttgg ttaagttgca tgttggagta atcctcgcag agataccacc
      121 atacttcgac agggagcgcg tgggagaagg tgcaaaacct ttagtccttg tctgcatggt
      181 cagcaaaacc atgtgcggat acgaccgatg cgtggctccg tcagcccaat tacaggttaa
      241 tcggcaatac ttaagaggat atttgatttt tttaaatcaa agcctcttag ggaaagtatt
      301 gccgaaactc tctcaacgtt caccatcagc ccgagcaaag cgagggatgt ggggacggtg
      361 agaaagagtt gagcgcattt gtttttacaa gattcatgcc aatacctact agagtttata
      421 taccgtgtca tttgattagt ttttattgaa tttcactcgt ccatttttaa ataagcaagg
      481 atacacagct acccctattt aacttcgtta aacagtgata gtctgtgacc ttgatcaggg
      541 gcttcacccc cgataccccc gaaaagcttt aaacagaaaa atcaaagatt ttccaattta
      601 aagcttacaa aagccaaagc agggtagatt gaaatgtcag gacaggaaca agaaaacaaa
      661 cagagcaaag cagttcgaga acgaaccttt aaagtcagac tttcagaatc agaatatcaa
      721 ttgctcgatt ccagtaatcc ttatggttcg attgcaaagt tgcttagaca agcagcagta
      781 gagaaagcga ctaaaattca taattccaaa gcagaagatg accaaaaaat taaagcaaca
      841 aaggttgttg aatcttcgta tagcaagaca gagcgagcat taattttaga gcttggtcgg
      901 attggcaaca atgtgaatca gattgctaaa gcagtgaata ccgatattgc aggggtaggt
      961 acttttgaca aagtgaagtt acttcatttg ttgatttcca tagatcagca gcttcgggag
     1021 ttacgtccag atgatcgttg atttctttag acatggttca ggtttatcaa aaggctgtct
     1081 cgattatttg ttaggtgaag atcgagagcg tgaacatgct caagtgctca atggtgatgt
     1141 agaactgaca gcacaactca ttgatagcag tccttttgcc aaaaaatata ccagtggttg
     1201 tttgtccttt tatgagcatg atttatctga ccaggataaa cagaaaatca tgcaaaattt
     1261 tgaggaatgt ttattcccgg gactagataa agaccaatat caaattttat gggtacagca
     1321 ccaagacaaa attaatcagg acacaggtga gacaaggctt gagcttaatt ttgtgattcc
     1381 taatgttgag ctttcaacag gcaaacgcct acagccattc tatgcgccag tcgatttaga
     1441 tcgagtcgat ctatttaagc agatcactaa taccgaacat tcactgtatg accccgatga
     1501 tcctgagcat cgacagctat ttttaaataa aaagaaccta cccaaagata ttaaggattt
     1561 taaagcgcag ttacatcaac gtgtttaccg agcggttgcc aatggtgatg ttgccgatcg
     1621 gcaagaattg gtgcaatggc ttgaatcgaa tcaaatcaat gtcactcgac aagtaaaaaa
     1681 ctccatatcc attgaaaacc catatgaggg cgcaaaacgt cccatccgtt tagagggcga
     1741 gatttatgag caaggcttta gagctactgg cgaatataga caagaagtac aacaacgaat
     1801 tgaagaatac agaggaacaa cttctgagcg gtatcgaaca aacgtcacag actatcagag
     1861 acagcttgaa cataagagcc aatatcactg cgaccgctat ccaacagtcg gacgagaaaa
     1921 tagcccagag cattcaaaac aacgcccaga cggtcgagaa gcagttaagc ctgtatcaaa
     1981 ccttgctgca atcgaaattg agccatttaa cgcaattaaa cgagcaaata cagagccaag
     2041 aacaacaagc ccagaatcaa gcggtacaga aaaaacttac cactttgaat atgggactga
     2101 tttcagtagc agttattttg cttatagcaa ttttctcgct tggtctcgtc accaaaaaca
     2161 agtacagcga gatgcagacg ctaaataccg tgatcaacga gaagcaaatg agggtcgacc
     2221 atttgaaccg atcgggaggc agtttgacga tcagcacatg caagcgacca gacagcaaag
     2281 cacagcgcct atgtattcag atcaacaaga acgcaggcga atggcagaac ggctacatga
     2341 ttccaatggg gtattaaacg atgaccgaat tagaaacaca attattgaga atcatcgaag
     2401 aacaacagca acaattaatg caacaacaag tgcaattgca gaagcaacag caagctttag
     2461 acacaatgca aaccaaaatt atccaagcct tgtcagcaca atcaaagaca atcaagagcg
     2521 atcagcaaag cttacagcaa gcacagaaat cattagcgac aatgcagaag caatttcacg
     2581 agctagaacg aagtacagca agctataccg agcagatcaa tggcaaagtc cgagagatga
     2641 gccttatcac agccaacaac gaagaaattc tggagagaat ctcaaccaca cagcattctg
     2701 tctcgtcaga agtagccaaa gcgcagcaga atttgctcga aacatccgag atattgaaaa
     2761 aaatctcgtc cagatgaatg agcgtaaaca agagatagaa aaacctcgac ctaagcaaga
     2821 tcgaggtatg gattttggga tgtaattatt ttctggtatg ttggtgttta tttcttagta
     2881 ttagcttgta ctaatgttta aatttagcta tattgaatgt agtataggct tgtttatata
     2941 ttttggtggt gttatgaata ataactgtgt agtagtacta actgctcgtg gaaagactag
     3001 aattttaaaa gaaggtggta gtcagtcttg gagactaaat gctcataatg caggaaagtg
     3061 cgattatcta gtttgtgtgc aaaataggaa acaatcttgg ggagaaccag aggctgatca
     3121 tcacactgca tttttagttg gcaaaatatc tagtatcagc caatctcaag aagaagatgc
     3181 agatggtaga tggataataa atatctccga atatgctgat atatctattc ccaatatgtg
     3241 ggatggtaat cggaatccag tttcttatag ttcattagaa gagatgaata tcgatttgaa
     3301 tgagctgaaa tttaccaaat taaatgaaga taattcttct aaaacactgg ataaaatatc
     3361 tattcctgaa gctaagagac tattagctaa ttattttgat gtccctcaag aaaatatttc
     3421 aattacaatc aatatgtaag cttttaattg accaaaaaaa gaaaaaagcc tttcacgtta
     3481 atgaaaggct tttttgaatc ttagcgcttg ggagggaaga tcttagatcc gcagtctctc
     3541 aaacttacaa ctatatatta gggtatatac ggtggaattg tcaaatgatg cagtgcttaa
     3601 gatagagcag gctttatcta taccacgctt atctaagtat gaaaatttct ataaagataa
     3661 aggtgaacca tatgaaaaat ctgatgtatt gatgctttat gaacgtaatt taataatttc
     3721 caataagttt ttttacttat taaattattt tgaagttgtt ctgagaaatg ctgttgttca
     3781 ggcaatagaa atatctttta ggtgtaatga aacaaatagt tggcatgaaa atgaagcttt
     3841 tattcgaagc cttagccgta gagggaggta tagccctaaa agtatgtttg atagtgctaa
     3901 agagaagttc ccagattcgc ctagtaaaat gatccctgag cttaaatttg tattctggca
     3961 aaagatgctt atggctaatt atgaagaacg tatttggcag ggatgtttta attctatttt
     4021 tcctaatgca acagttgaaa acaggcaaat tttttacgat tggacagatc aattacgtga
     4081 attaagaaac aggattgctc atcatgaacc gattattttt aaccgtaatc tagaaaatga
     4141 tttagaggtt ttaacaaaat ttatttactg tcgttgtatt aatacatacg attttatcga
     4201 gcaatctgca ttagatctaa aagaatattt agatttttaa gataagaatt tcaccccaaa
     4261 caatcgaaag gtcagactat tttgcaaatg ttgaattaac aaggttagta gtaaagcagt
     4321 aaatccgata acggtagcag tgtttcctaa cacctgagtt aagccgcgcc gcaaagcggc
     4381 gtcggcttgg acgaattgtt aggccgcata tcgcgacctg aaagcggcac gcaagacctc
     4441 aaccttttcc gccccgagtg aggtgcatgc gaacctgtag gactctatgt gctttgtagg
     4501 ccagtccact ggtggtactt catcggcata gtaaaagtaa tcccagatga tcgcctccca
     4561 gctgttacaa cggactggcc gcccggcgat gacaccctca gccgcctctg ggcacgagcc
     4621 ctgcggagcc tccgcgattt catacgcttc gtctgcccac caagcaggtt cgcagtcaag
     4681 taactcatcc ccgatctccg ctaagaatcc atagtccaac tcctccgtga cgcgcccgcc
     4741 gagcatttca actattgcct cgagctcgcc gcgcctctcg ccgggaaaag tcagatcaat
     4801 atcatcgtgc ttgcgtgtta cacgccctag ccgtgcatcg atcgcccagc ccccaccgat
     4861 ccagagcggc agatttcgct catctgccgc agctagaatt tggtgtatca atgcgacctg
     4921 cgttgtgtcc atgcggccta acatagtaag ccctcccaga atgtcagtaa ttagactatt
     4981 aattgaattg ttacatccaa tagtgagctc ccagtgattg tcttaactat ccaacctttc
     5041 gattggatgg ggcagagcac cattattctg tttgaatgcg gtgttagtgt caactaggtt
     5101 ctttatttct tcaaggagtt tgaaagtgct aagtattttt actttacttt gttcctcaat
     5161 ataaattttt ttaagaccat ttagcagctt gtatttggat attttggata ggagaatgaa
     5221 taaggttgcg gtattgattc ttcccccttt tagatgaagt aaacggtttt tttgcagtat
     5281 tggaatattt ctatattctg ttagttgatc ttttaagtca tcaggaattt tcatttcgta
     5341 aaaattatat tttttagaac agtctaatat cgagtgattt aggctttctg tatttgtatt
     5401 aataaaaatg catttttcaa atgtgcaatt tttaaagcga caatctctaa aaatagcagc
     5461 ataaaatacg cactttttga agtgaacatt cgtaaaccta ttcttattta agattactcc
     5521 tgaaaaatca gataaaacaa tttttatatt tttgaatgaa catgatcgta ggcgggatcc
     5581 tctcaaatta acattaaaaa atgttttatt agagaaacgt actttagaag aaatagattt
     5641 ggctaggagg ctatttgaat acttacgttt ttcgatgtgc ttgttatgaa atctaggaag
     5701 ttttgacatt aatagtccaa agaataggag tttcagctta attcttaggt gtaatcaaaa
     5761 aaaataatgg acaggtctct tgcgaaatct gtccattagt tttaaattcg atgggtacag
     5821 ttatttgatg cttgcaacat caattaacga aagatcggca gagcttgcaa ctcatactga
     5881 tattcacctt aaaggctagt accactatct attttaaaga attagaacag tttgacaagc
     5941 ctcaatttga agtccttcgt taattcagca gtttaaatca tcaaataagc acctaacggt
     6001 cgagctttat ctacttctgt gcgccttttt taaggaagat atgcgttgca tgtggaggga
     6061 ataagtgtca ctggtgca
//
