LOCUS       NZ_CP123435             2476 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Klebsiella pneumoniae strain SH22PE16 plasmid pYUSHP16-5, complete
            sequence.
ACCESSION   NZ_CP123435
VERSION     NZ_CP123435.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34239926
            Assembly: GCF_029867025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Wang,J.
  TITLE     Complete sequence of Klebsiella pneumoniae SH22PE16
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2023) Key Laboratory of Prevention and Control of
            Biological Hazard Factors (Animal Origin) for Agrifood Safety and
            Quality, Ministry of Agriculture of China, Yangzhou University,
            No.48 Wenhui East Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029867025.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,421
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,137
            CDSs (with protein)               :: 5,137
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 60 of 163
            Pseudo Genes (incomplete)         :: 110 of 163
            Pseudo Genes (internal stop)      :: 23 of 163
            Pseudo Genes (multiple problems)  :: 27 of 163
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123435.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SH22PE16"
                     /isolation_source="pig feed"
                     /db_xref="taxon:573"
                     /plasmid="pYUSHP16-5"
                     /geo_loc_name="China: Shanghai"
                     /lat_lon="30.40 N 120.52 E"
                     /collection_date="2022"
                     /collected_by="Yue Jiang"
     gene            join(2310..2476,1..475)
                     /locus_tag="QEZ34_RS27045"
     CDS             join(2310..2476,1..475)
                     /locus_tag="QEZ34_RS27045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094307618.1"
                     /translation="MRLKNWLIPLVAWIVIFMGLKLILGGGCNDGWGSSSIGRMGACSH
                     HGGVNHTPGFIAFFISTAIAAYLFFKIDEMDTRKIKNAHSIQASYFEMESTSAPFNPCY
                     SLRLSSKEINFTHSSSWDGDKTVINIPSSPEELKYILSLSEKIKKDIENFREENTTFGC
                     DGEFVSIKTFNGSQEMSFVTPMLFISFESISPATLEMMTYLRNRLGFYLH"
                     /name="hypothetical protein"
     gene            810..1046
                     /locus_tag="QEZ34_RS27050"
     CDS             810..1046
                     /locus_tag="QEZ34_RS27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_096768690.1"
                     /translation="MNRLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREGERQQSEFAWSRPRAPRS"
                     /name="hypothetical protein"
     gene            1429..2313
                     /locus_tag="QEZ34_RS27055"
     CDS             1429..2313
                     /locus_tag="QEZ34_RS27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094307619.1"
                     /translation="MRNAALELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPENGHAHLLYALKTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADTGYSGLICKNPNHSHWKIAVWQPELYTLDWLADYLDLTTA
                     NDKEIVADYGLGRNCTLFDKTRRWAYRAIRQGWPEYDQWLQACYERASAYNLQFALPLD
                     ENEVMGIAKSIAKWTECNFSKDLFLEYVKNTHSSEIQSKRGKMSKGGGRKSLGEPWVEL
                     GVSRATYFRNRKK"
                     /name="replication initiation protein"
ORIGIN
        1 tgcttttttc atttccacgg ccattgccgc ctatcttttt tttaaaatag atgaaatgga
       61 tacaaggaaa ataaaaaacg cccatagcat tcaagcaagt tattttgaaa tggagtctac
      121 tagcgctcct ttcaatcctt gctattcgtt aaggttaagt tcaaaggaaa tcaatttcac
      181 tcacagttct agttgggatg gtgacaagac tgttattaat atcccatcct cccccgagga
      241 gttaaaatac attctatctt tatcagagaa aataaaaaaa gacattgaaa attttagaga
      301 agaaaacact acttttggat gtgacgggga gtttgtgtcc attaaaactt ttaatggctc
      361 ccaagagatg agctttgtta ccccaatgct attcattagc tttgaaagta tatctcccgc
      421 aacgcttgaa atgatgactt acctaaggaa taggttaggt ttttaccttc attgaaggtc
      481 atcttagctt tagtgagact aaacaaccta tatcagataa cagccctctc tagcagaggg
      541 ctgttttttt gcagccagca tagaatctga catactgcac ctatcttcag ccagtataca
      601 ctccgctagc gctgcgtgac tggttcaggg ctgcgccccg aaacccgctg aaaagcactg
      661 tcgcgcctgc ggcttgccca acgtctcacg tcaccggatt aaaatccgct cccgttcacc
      721 gtttctggct gtctttcagc accgccagcg cttcatcggt tctgctgtgt ctggcagact
      781 gtgcggagtt attctggcgg gagaatgcta tgaacagact gacaaacctg acacctgcag
      841 agaaaaaatt tcttgatgat gctgttgccg cagctgagag agcgtcaggc aaaaagctaa
      901 atcagccgaa ccggcatatc gtcctgaaca gagcgcgtgc gcagattgag tcgcagcgtt
      961 acgccgaccg ccagcgtgca ttacgtgagg gtgagcggca gcagtccgag tttgcctggt
     1021 cacgtccgcg cgcgccgcgc agctgattgc gccttttggc ggtttagtgg tgctgacact
     1081 ccgaaatcgt ctgccattca cccgcgtgca tagccatgca gtcgcgcaga aatcatcagg
     1141 tgcgtttttc agggttatgg ccagggaaag gtgctggaaa tggctaactc gttacagagt
     1201 ggcgctgagg gcctttcaga gggtgcgccc aacggatgtt atggtaaatc gtggtgagat
     1261 cgacagacgg ggaaagacgg tgtaacgtta ggaccagaaa aaacaaagcc ccggcatcat
     1321 gtctaatctt ggcggatccg catgataacc agggccaggt cgaaaccttg aaaggatatt
     1381 agcacatgaa ccgtgccaga caacagcccg ccataagcgc agggcacatt gcgtaacgcc
     1441 gcactcgaac tttttaacga ccgcttaccg cacaagccgt acttctcgga cgatctgcac
     1501 ttcggtgtac gcatcgcagg gaaagagcga gctatcctgg cgaaatacat ccagttcaat
     1561 cagcctcacg ccatgttctg gctgggattc gatgtcgatc gcgccggtgc cgccatagac
     1621 tggagcgacc gcaacgcgcc cgcgcccacg ctgaccatca ccaatcccga aaacggacac
     1681 gcgcacctgc tctatgcgct gaaaacttcg atccgtactg cgccagacgg aaaaatgaag
     1741 ccgctgcgct acgccgccgc tgtagagaac gcgctacgta aaaaattaga ggccgatacg
     1801 ggttattcag gactaatctg caaaaacccg aaccatagcc attggaaaat tgccgtgtgg
     1861 cagccagaac tgtacacact ggactggcta gctgactatc tcgacttgac caccgcgaac
     1921 gataaagaaa tcgttgctga ttacggctta ggccgtaact gcactttgtt cgataaaacc
     1981 cgcagatggg cataccgagc tattcgtcag ggctggcctg aatatgacca gtggctgcaa
     2041 gcctgctatg aacgtgccag cgcttacaac ctgcagtttg cattacctct ggatgaaaat
     2101 gaggttatgg gaatagcaaa aagcattgct aaatggactg agtgtaattt cagcaaagat
     2161 ttgtttttgg aatacgtaaa aaatacgcat tcttcagaaa tacaatcaaa aagaggcaaa
     2221 atgagtaaag gtggaggtag aaagtctctt ggcgaaccgt gggttgaatt aggtgtaagt
     2281 agagctactt actttaggaa tcggaaaaaa tgagattgaa aaactggctt atcccacttg
     2341 ttgcatggat tgttattttt atggggttga agttaatttt aggtggcggg tgcaatgatg
     2401 gatggggttc gtcctcaata ggaaggatgg gggcctgctc tcatcatggc ggtgttaacc
     2461 ataccccagg cttcat
//
