LOCUS       NZ_CP123434             3223 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Klebsiella pneumoniae strain SH22PE16 plasmid pYUSHP16-4, complete
            sequence.
ACCESSION   NZ_CP123434
VERSION     NZ_CP123434.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34239926
            Assembly: GCF_029867025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Wang,J.
  TITLE     Complete sequence of Klebsiella pneumoniae SH22PE16
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2023) Key Laboratory of Prevention and Control of
            Biological Hazard Factors (Animal Origin) for Agrifood Safety and
            Quality, Ministry of Agriculture of China, Yangzhou University,
            No.48 Wenhui East Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029867025.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,421
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,137
            CDSs (with protein)               :: 5,137
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 60 of 163
            Pseudo Genes (incomplete)         :: 110 of 163
            Pseudo Genes (internal stop)      :: 23 of 163
            Pseudo Genes (multiple problems)  :: 27 of 163
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123434.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SH22PE16"
                     /isolation_source="pig feed"
                     /db_xref="taxon:573"
                     /plasmid="pYUSHP16-4"
                     /geo_loc_name="China: Shanghai"
                     /lat_lon="30.40 N 120.52 E"
                     /collection_date="2022"
                     /collected_by="Yue Jiang"
     gene            join(3123..3223,1..244)
                     /locus_tag="QEZ34_RS27020"
     CDS             join(3123..3223,1..244)
                     /locus_tag="QEZ34_RS27020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_032074402.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            453..>887
                     /locus_tag="QEZ34_RS27025"
                     /pseudo=""
     CDS             453..>887
                     /locus_tag="QEZ34_RS27025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            922..1407
                     /locus_tag="QEZ34_RS27030"
     CDS             922..1407
                     /locus_tag="QEZ34_RS27030"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1411..1626
                     /locus_tag="QEZ34_RS27035"
     CDS             1411..1626
                     /locus_tag="QEZ34_RS27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1794..2126
                     /locus_tag="QEZ34_RS27040"
     CDS             1794..2126
                     /locus_tag="QEZ34_RS27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_016807542.1"
                     /translation="MNTYIFLGGAIVAEVIGTIFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     ARG             1795..2126
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          2857..3048
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(2394..2874)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          235..1724
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcggcagacg tgcctggacg agaagcccgc acgcgccggc aaacttccgt cgatctcgcc
       61 ggcgctgctt cgtcagcttg ccggcatggg gaacaacctt aaccagattg cccgccaggt
      121 taacgccggt ggtggaagcg gacacgaccg cgtgcagatt gtcgccgcgc tgatggccat
      181 cgatgccgga ctcgagcggt tgcggcatgc cgtactggaa aagggtgctg atgatgatcg
      241 ttaagtttca tcctcggggg cgcggcggcg gcggcgggcc ggtcgattat ctgctgggta
      301 aagatcgcca gcgcgacggc gccagcgttc tgcaggggaa gccggatgaa gtccgggagc
      361 ttatcgacgc ctcgccctac gccaaaaagt acacttccgg cgttctgtct tttgccgaac
      421 aggatttacc gcccggccag cgcgaaaagc tgatggcgag tttcgagcgg gttctgatgc
      481 ccggactcga taaagaccag tacagcgtgc tgtgggttga gcaccgggac aaggggcggc
      541 tggagctgaa cttcctgatc ccgaacacgg agcttctgac cggcaagcgg ctacagccgt
      601 attacgaccg cgccgaccgt ccgcgcatcg atgcctggca gaccatagtg aacggcaggc
      661 tggggctgca tgacccgaac gcgccggaga accggcgggt gctggtctcg ccttccgcgc
      721 tgccggaagc gaagcaggaa gccgcccagg cgattacgag cggcctgctt gcccttgcct
      781 catccgggga gctgaaaacg cgtcaggacg tcactgaggc gctggaaagc gcaggttttg
      841 aggtggtgcg caccacaaaa agcagcatca gcattgccga cccggacggg gggcgaaaca
      901 tccgactgaa gggagccatc tatgaacagt cttttaacgc tggcgaagga cttagagcag
      961 aaatcgaaag cgcagcagca gactaccggc gagatgctga aagccgcatt cagcgagcac
     1021 gagaagtctg tcagagcgga actgagcgaa agcgagaaga gaatcagcgc cgccatcctc
     1081 gaccacgacc ggaagctgtc ctcagccatg agccagcgca cgaaagggat gctgcgcatg
     1141 gtcagccaga cgtggctgac catcgttctg gtctccgcgc tgctgatagc gtcgagcgcg
     1201 ggcattctgt ggtggcaggg gcagcagata ctcgagaact atacgaccat ccgggagcag
     1261 aagagcacgc aggccatgct gtcagagagg aacagcggcg tacagctctc gacctgcggc
     1321 gagcagagac gccgctgcgt gagggtgaac ccggaagcgg gacagttcgg agaggactcg
     1381 agctggatga tactggcggg gaaatagcac atgacggagc tggaaaaaca gttgctgagc
     1441 gcattagagc agctacagca ggactactcg aaaaggctgg acgagtggga gaacgccttc
     1501 gcggaatggc ggacgatgtc tggtcttatt caacgggaga acgcggcgct gaacgagcgc
     1561 gtcacggtct tgagcaggca ggtgcagagt ttgagcgagc agctgcgccg gttgtcgaaa
     1621 ggctgaacgc gatagaaact caccggcagc aggagcgggc ggtgcagcac cagaaagcgc
     1681 tggagctgga acgatcgcag tggcagcatc atggtccttc gctatgataa gccgggttct
     1741 attaattagt tgttatgtaa agatatttaa atttttcaac atgaggtatt tttatgaata
     1801 catatatttt cctgggaggg gctattgtgg ctgaagtcat aggtaccatc tttatgaaat
     1861 attcagaagg atttacacgc ctgtggccgt ctgtaggaac tgcaataagt tattgcgctg
     1921 ccttttatct gctttcccaa acattggaaa atatacctac cggtattgct tacgcaattt
     1981 ggtctggtgc gggaattgta ctaataagcc tagctggttg gttggttagc ggtcaaaaac
     2041 ttgattggcc cgcaattgca ggaatgttcc ttatttgcgt tggagttttg gttattaata
     2101 ttttttcaaa atcttctgcg cattaacttt ttaccgaagc atattggcca aagcaaaagg
     2161 atttccttga gatcctttct ttctgcgcgt aatctttttc cctgtaaaca aaaaaaccac
     2221 cgctaccaac ggtggttcgt ttgccggaat tcagagctac caactctttg ttccgatggt
     2281 aactggcttt cacggagcgc agatacctaa actgtccttt cagtgtagcc gcactcaggc
     2341 tttcacttca agaactctgt aggcattctc tacaatctct gctccgtgaa cccagttacc
     2401 aatggctacc gccactggcg atttgtcgtg tctttccggg ttggactcaa gttgatagtt
     2461 accggaacgg gcgtggtagt cggactgaac ggggggttcg tgcatacagt ccatcctgga
     2521 gcgaactgcc tacccggaac cgagtttcag gcgtggaatg agaaaccgcg gccataacag
     2581 cggaatgaca ccggtaaacc gaaaggcagg aatgcggggg agcacgaggg agccaccagg
     2641 gggaaacgcc tggtatcttt aagccgcatc gggtttcgcc accgctgatt tgagcgtcat
     2701 tttcgtgata ctcgtcaggg gggcggagcc tatggaaaaa cggcaactcc gcatcctttt
     2761 cttggccttt cctgcttcga gcggcatttg cttatcccgt aatctgtgag ctggtaacgc
     2821 ccgccgcagt ctcacggcag gagcgtagcg accgagtgag cgaggaggcg tcatttcatc
     2881 accacctgca ttttgcactt acgcccggca gccgcatttt tcccctgtca catgaagcac
     2941 tttcataaat tgccactccg tcataacatg tttagccagt atacactccg ctagcgctac
     3001 gtgactggtt cagggctgcg ccccgaaacc cgctaaaacc actgccgcgc ctgcggcttg
     3061 tccaacaccg tgccgaccgg gaaaagattt cccgtccgtc ccggcgttat caggaggccg
     3121 gattggcaga caaacgcagc aaaatgctca ccatgtgggt gactgaggac gagcaccggc
     3181 gtctgctgga gcgctgcgag ggtaagcaac tcgcggcctg gat
//
