LOCUS       NZ_CP123038             3376 bp    DNA     circular CON 31-MAY-2024
DEFINITION  Escherichia coli strain E22 plasmid pE22P2, complete sequence.
ACCESSION   NZ_CP123038
VERSION     NZ_CP123038.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34164682
            Assembly: GCF_029854555.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Li,J.
  TITLE     CRISPR-Cas Diversity and Phylogenetic Distribution of Human
            Carbapenem-resistant Escherichia coli Isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2023) Graduate School of Guangzhou Medical
            University, The First Affiliated Hospital of Guangzhou Medical
            University, No. 195, Dongfeng West Road, Yuexiu District, Guangzhou,
            Guangdong 510030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.0.9
            Assembly Name                     :: E22_complete genomics
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10X
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029854555.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 06:55:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,810
            CDSs (total)                      :: 4,694
            Genes (coding)                    :: 4,438
            CDSs (with protein)               :: 4,438
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 90 of 256
            Pseudo Genes (incomplete)         :: 159 of 256
            Pseudo Genes (internal stop)      :: 54 of 256
            Pseudo Genes (multiple problems)  :: 38 of 256
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123038.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E22"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pE22P2"
                     /geo_loc_name="China:Guangdong"
     gene            complement(334..525)
                     /locus_tag="QEN54_RS23985"
     CDS             complement(334..525)
                     /locus_tag="QEN54_RS23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            589..912
                     /locus_tag="QEN54_RS23990"
     CDS             589..912
                     /locus_tag="QEN54_RS23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1121..>1558
                     /locus_tag="QEN54_RS23995"
                     /pseudo=""
     CDS             1121..>1558
                     /locus_tag="QEN54_RS23995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1590..2150
                     /locus_tag="QEN54_RS24000"
     CDS             1590..2150
                     /locus_tag="QEN54_RS24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067486.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGMKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2157..2390
                     /locus_tag="QEN54_RS24005"
     CDS             2157..2390
                     /locus_tag="QEN54_RS24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          170..253
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.059"
                     /coverage="95.0"
                     /evalue="8.83e-18"
     Typing          903..2473
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.439"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgaccgaacg tagtgagcga gtgggcgagg aagcggaaga gtcacagagg caagcattgt
       61 gcacttacgc gcctgtgcca gtgtccgcgt gtgtgttgtt tatcgggcgc gctgttttta
      121 cgcatctgtg cggcttttta ctcctgacat ggtgcacttc ccatccactc tgcaccgatg
      181 caaaatgagg taagccagta tacacatcgc tatcgctctg tgaactgggt cagggctgcg
      241 ccccgacacc cgcactgacg cgccctggcg ggcttgtctg ctcccggcat ccgctcacag
      301 gcaagctgtg aacgtctccg ggagctgcat gtttcagttg ctttcaccgt catcgccgaa
      361 gcgtgcgagg caactgcggt agagttcgtc ggcgtggtcg tgaagtgatt cgcagatgtc
      421 tgcctgttcg tccgcgtcca gatcgttgag tttttccagc agggttagcg tctggcttct
      481 gataaagcga gccatgttaa gggctgtttt ttcctgtttg gtcatggtct taaggctcct
      541 tgtatggggg atttgttagg gggaatgata ccgatgaaac gcgagaagat gctcacgata
      601 cgggttaccg atgaggagca cgcccggtta ctggaacgct gcgagggtaa acgactggcg
      661 gaatggatgc ggcgggtctg tctgggggag ccggttgcac gcacaggaaa actgccgaca
      721 ctggcaccgc cattgctgcg gcatctggct gccatcggga acaacctgaa ccagacagcc
      781 cggaaggtga acagcggcca gtggtcttcg attgaccggg ttcatgtggt ggccgcactg
      841 atggccatcg agggtgaatt gcgccagttg cggcaggctg tcagggaaca gggggtacgg
      901 gatgatagtt aaatttcatg ccagggggaa aggcggtgga agtggcccgg tcgattacct
      961 gctggggcgg gagcgtaacc gcgaaggcgc aacggtgctc cggggtaacc cggaagaagt
     1021 gcgggaactc attgattcca cgccattttc gaagaaatac acgtcaggcg ttctgtcgtt
     1081 cgcggagaag gagctaccac cgggtgaacg cgaaagagtg atgacgagct ttgagcgtgt
     1141 tcttatgccc ggtctcgaaa aagaccagta cagcattctc tgggtggaac accaggataa
     1201 ggggcggctc gaactgaatt ttgtcatccc gaacatggag ctacagagcg gtaagcgcct
     1261 ccagccgtac tacgaccgtg cggacaggcc gaggataaat gcctggcaga cgctggttaa
     1321 tcatcattac gggctgcgtg acccgaacgc accggagaac agacggacgc ttgtcacgcc
     1381 aaataatctg ccaaaagcga aacaggaggc cgcagaagcg attacacggg gtcttgaggc
     1441 tctttaccat gccggagcaa taaaaacgcg tcaggatgtc acagaggcgc ttacagcggc
     1501 aggttttgag gtggtgagga caaccaaaag cagcatcagt attgccgacc cggagggggg
     1561 gcggaatctc aggctaaagg gggcgctgta tgagcaatct tttacaaatg ggaacagact
     1621 tcgagaagaa actgaaagag cgagccgctt ctaccgagaa catgctgaac agcgagttca
     1681 ggaagctcga agagtctgta gacaaggcat tgagcttaaa caggcagaaa atcagagacg
     1741 ctatcagcga acatacaaca tccgtgaaaa agcaactgga tacgctgagc accaccgtga
     1801 gcatgcagtt cagcaccaca gaggccgaac tgtcccggca gcagaaaaaa ctgctctggc
     1861 gggtgatcaa agggcggata ctgtttccgg cgctgaccgc gctcagcgtc acgggcggga
     1921 ttttcctggg gtgctggggg ctgatggagt ggcaggaaag caagattgcg aaaaatatcc
     1981 tgactatcag agagcaggaa aacacgctgg cgaaactcga ggcaaaaacc tggggggtga
     2041 cgttcgtcaa tggcgagaac gggaaatttc tggtgttgcc ggacgggatg aaaggggaaa
     2101 acacctggac ggtgggcgac aagaacgccg tgaggctggt cagggagtaa acgagtatga
     2161 cggaactgga agggcatttg ctgaacgcct tagagcattt gcaacaggac tatatgcggc
     2221 ggctgaacga gtgggagagc gccttcgcgg aattgcagaa gatgcacgag gttacgcaac
     2281 ggaacaacgc gatactgaac gagcgcgtcg tgatattgag tcagcaggtg cagagattag
     2341 ccgggcaagt ggatcgcttg agtcggttat ttatcgcgaa caacagctaa gggatgaaca
     2401 ggagcgtgcc cggtacagac atgaggtaca ggaacgcgcg gcacgggttc ggagtgggcc
     2461 atcaatggag atgtgaccgg ggaagtgacg aaatcttgcc attttgaccc gttcgacggc
     2521 tggggcgagc gaaacaaatc gtgtgccgta tttgtgcata gctatgcaac atcctgaaaa
     2581 cgcatttcac gcgtttttgg ggattcagcc aggggaaaac atggtttttt tactttttga
     2641 gcgtaaagac gtgaaaaatc tcgttattgc acttgcgatc tttcgtcggc gggcgtagaa
     2701 cgaaaacagc tcgccaggaa ggcgagctgt ttcgggttca ttgaggtctc tgacctcctc
     2761 aatgttcctg tggaaactgt tgagatccac aacagaaacc agttcattca gtttgcatga
     2821 gccttctgta ttcttcaaga atttttcaac agcacagtct cccaggaacc ccgaaaaaaa
     2881 cctgtttttg cagatacttg cgtatttgtg agaatcgttt gcggttgggg gagctgtgac
     2941 gacggcgacc gcacgcgtga gagttccgct accggcgcag agagtttcat cgacagcgga
     3001 cacctgatga gttgccacaa aaaccgtatg tagcgcggct agattggttg agttagacct
     3061 tggcggcagt tcccggtatg tggtggcgtg acgggtgaga tggtgcggcg aagacgcata
     3121 cgtcacgcag gcgcgggttc ttccactgac gcgccccgtg atcgatggcc ttggctccgt
     3181 ccggcatatc gagagcggca aactacccgt cccttcactg ggaggggtga gttgttgccg
     3241 ttccctccga ccttccacca tccagcatat cgcacgatcc catgtgcacc gctactcctg
     3301 ataactccag tgcaaaaaaa aaaaaaacgg atctgcatca caacgagagt gatcccaccg
     3361 ctcgccgcag ccccag
//
