LOCUS       NZ_CP123034             2088 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli strain 20-20 plasmid p20-20P5, complete sequence.
ACCESSION   NZ_CP123034
VERSION     NZ_CP123034.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34164680
            Assembly: GCF_029854535.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Li,J.
  TITLE     CRISPR-Cas Diversity and Phylogenetic Distribution of Human
            Carbapenem-resistant Escherichia coli Isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2023) Graduate School of Guangzhou Medical
            University, The First Affiliated Hospital of Guangzhou Medical
            University, No. 195, Dongfeng West Road, Yuexiu District, Guangzhou,
            Guangdong 510030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.0.9
            Assembly Name                     :: 20-20_complete genomics
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10X
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029854535.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:34:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,886
            CDSs (total)                      :: 4,766
            Genes (coding)                    :: 4,516
            CDSs (with protein)               :: 4,516
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 91 of 250
            Pseudo Genes (incomplete)         :: 152 of 250
            Pseudo Genes (internal stop)      :: 55 of 250
            Pseudo Genes (multiple problems)  :: 39 of 250
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="20-20"
                     /isolation_source="Bronchoalveolar Lavage Fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p20-20P5"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2020-06-12"
     gene            223..1188
                     /locus_tag="QEN57_RS24390"
     CDS             223..1188
                     /locus_tag="QEN57_RS24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039064478.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1192..1458
                     /locus_tag="QEN57_RS24395"
     CDS             1192..1458
                     /locus_tag="QEN57_RS24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          617..848
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 cccagtaatg acgaggcttt gagtgggttt tgacaggtca aagaaaatgg agcagaattg
       61 aggcgttttt aatcggcgtt ggggagtgcg tcaacactcc ccaacatttc gaatgtgtca
      121 cctcagcggc aaactctggt gacatgtact ggctcgcaat gcacaggtac gtgatgaata
      181 taccacatca aatcacagcc tgcccagatc ggagcaggct taatgtcaga agataaattc
      241 ctttcggact acagcccccg tgatgcagtt tgggataccc agcgcacgct taccgattct
      301 gtcgggggta tctaccagac tgctgctgaa ttcgagcgct atgcactccg tatggcctcc
      361 tgtagcggtt tgttacgttt tggttggtct accatcatgg aaaccggaga aacgcgccta
      421 cggcttcgta gtgcgcaatt ttgccgtgtc cgtcattgcc ctgtctgcca gtggagaaga
      481 accctcatgt ggcaagcccg tttttatcag gctctaccga aaatcgttgt ggattacccg
      541 tcttcccgat ggttgtttct gacgttaact gtcaggaact gcgagatagg tgaacttgga
      601 acagtcctta cagcaatgaa tgcggcgttt aagcgaatgg aaaagcgaaa ggagctatca
      661 cctgttcagg ggtggatcag ggctacggag gtgacgcgag gtaaggatgg cagcgcacat
      721 ccgcattttc actgtctgct gatggtgcaa ccttcttggt ttaaagggaa gaactacgtt
      781 aagcacgaac gttgggtaga actctggcgc gattgcttgc gggtgaacta tgagccgaat
      841 atcgatattc gggcagtaaa aactaagaca ggtgaggttg tggccaacgt tgccgagcaa
      901 ctgcaaagcg cggttgctga aacgctgaaa tactccgtta aaccggaaga tatggcaaac
      961 gatcctgagt ggtttcttga gctgacgcgg cagcttcaca agcgccgttt tatctcgacc
     1021 ggtggagcgc taaaaaacgt cctccagttg gatcgagaaa ccaatgagga tcttgccatt
     1081 gccgacgatg taggggatgg cactgatgac gggaagcgga cggcgtttgt ctgggattca
     1141 ggtaaacggc gttacaaacg cgcccctgag aaggataaat cggattaacg tatgaatatt
     1201 aatattgaat acctgaatgg aaataagact attggtttat tttttttaag aagtgaagcg
     1261 gtgattcctg acaggtttaa aaaccttatt ttgcttattg atggattaag ttttggcaca
     1321 tttggttttc atccgcacga aggttttgag gatgaattaa ttttatatat tcagaaaaca
     1381 aacgagaggg taaaaactct ttttgtgaaa atagatttaa acggtatcaa gtttggattt
     1441 ttaagaacgc attcttagtt ctggaaaaga gccagcggca ggctgaggtg ataggtacga
     1501 gattgcatgc aatctctagt gctctgtcta tcctgcatta tcctcagcat tatcctcagc
     1561 cttgccaact cgacaccaat gcaggataga caatccgatg tcaaatgtta acactctgcg
     1621 agtggtacat tttccccgga ttatcgtcct gagcctgccg ctggctctct ttctaccgcc
     1681 tcgctttgct cgttgctcaa cgcctcacag acacggatta aaatccgcat ccgttcaccg
     1741 ttttttaaag tccgttaaaa gcatgatgcc atctccgaga gttaatctcg tcaaatgcta
     1801 aatcgtgggg gtcccctttg gggttccgat ttagtgattg acgacaccac cgattaaaaa
     1861 acttatgcgg ggtggatggt ttcacgaagt gaggccatcc acctgtaaga cagggttttg
     1921 tttttatccc ctgttttggt gatcgggtgt gtggaaaagg ttggggtaag ccgttcgggg
     1981 tgcttgtttt ggggggttaa aattgtggtt attttttgcg caattctcgc gcgtgatcct
     2041 tgtatttata cttaagggat aaatggcggg tatgaaatag tggtttag
//
