LOCUS       NZ_CP123026             3373 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli strain 20-16 plasmid p20-16P2, complete sequence.
ACCESSION   NZ_CP123026
VERSION     NZ_CP123026.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34164624
            Assembly: GCF_029854515.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Li,J.
  TITLE     CRISPR-Cas Diversity and Phylogenetic Distribution of Human
            Carbapenem-resistant Escherichia coli Isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2023) Graduate School of Guangzhou Medical
            University, The First Affiliated Hospital of Guangzhou Medical
            University, No. 195, Dongfeng West Road, Yuexiu District, Guangzhou,
            Guangdong 510030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.0.9
            Assembly Name                     :: 20-16_complete genomics
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20X
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029854515.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:35:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,819
            CDSs (total)                      :: 4,703
            Genes (coding)                    :: 4,449
            CDSs (with protein)               :: 4,449
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 90 of 254
            Pseudo Genes (incomplete)         :: 157 of 254
            Pseudo Genes (internal stop)      :: 54 of 254
            Pseudo Genes (multiple problems)  :: 38 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123026.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="20-16"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p20-16P2"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2020-05-22"
     gene            complement(join(3219..3373,1..364))
                     /locus_tag="QEN53_RS24025"
     CDS             complement(join(3219..3373,1..364))
                     /locus_tag="QEN53_RS24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_052969336.1"
                     /translation="MKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDAISEHTTSV
                     KKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTALSVTGGIFLGCWGL
                     MEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVLPDGVKGENTWTVGD
                     KNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<252..1094)
                     /locus_tag="QEN53_RS24140"
                     /pseudo=""
     CDS             complement(<252..1094)
                     /locus_tag="QEN53_RS24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1084..1407)
                     /locus_tag="QEN53_RS24035"
     CDS             complement(1084..1407)
                     /locus_tag="QEN53_RS24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1471..1662
                     /locus_tag="QEN53_RS24040"
     CDS             1471..1662
                     /locus_tag="QEN53_RS24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_062914769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2979..3212)
                     /locus_tag="QEN53_RS24045"
     CDS             complement(2979..3212)
                     /locus_tag="QEN53_RS24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1744..1824)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.585"
                     /coverage="93.0"
                     /evalue="4.04e-16"
     Typing          complement(4..1094)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.154"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2897..3372)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="99.0"
                     /evalue="2.57e-63"
ORIGIN
        1 gctctctgat agtcaggata tttttcgcaa tcttgctttc ctgccactcc atcagccccc
       61 agcaccccag gaaaatcccg cccgtgacgc tgagcgcggt cagcgccgga aacagtatcc
      121 gccctttgat cacccgccag agcagttttt tctgctgccg ggacagttcg gcctctgtgg
      181 tgctgaactg catgctcacg gtggtgctca gcgtatccag ttgctttttc acggatgttg
      241 tatgttcgct gatagcgtct ctgattttct gcctgtttaa gctcaatgcc ttgtctacag
      301 actcttcgag cttcctgaac tcgctgttca gcatgttctc ggtagaagcg gctcgctctt
      361 tcagtttctt ctcgaagtct gttcccattt gtaaaagatt gctcatacag cgcccccttt
      421 agcctgagat tccgcccccc ctccgggtcg gcaatactga tgctgctttt ggttgtcctc
      481 accacctcaa aacctgccgc tgtaagcgcc tctgtgacat cctgacgcgt ttttattgct
      541 ccggcatggt aaagagcctc aagaccccgt gtaatcgctt ctgcggcctc ctgtttcgct
      601 tttggcagat tatttggcgt gacaagcgtc cgtctgttct ccggtgcgtt cgggtcacgc
      661 agcccgtaat gatgattaac cagcgtctgc caggcattta tcctcggcct gtccgcacgg
      721 tcgtagtacg gctggaggcg cttaccgctc tgtagctcca tgttcgggat gacaaaattc
      781 agttcgagcc gccccttatc ctggtgttcc acccagagaa tgctgtactg gtctttttcg
      841 agaccgggca taagaacacg ctcaaagctc gtcatcactc tttcgcgttc acccggtggt
      901 agctccttct ccgcgaacga cagaacgcct gacgtgtatt tcttcgaaaa tggcgtggaa
      961 tcaatgagtt cccgcacttc ttccgggtta ccccggagca ccgttgcgcc ttcgcggtta
     1021 cgctcccgcc ccagcaggta atcgaccggg ccacttccac cgcctttccc cctggcatga
     1081 aatttaacta tcatcccgta ccccctgttc cctgacagcc tgccgcaact ggcgcaattc
     1141 accctcgatg gccatcagtg cggccaccac atgaacccgg tcaatcgaag accactggcc
     1201 gctgttcacc ttccgggctg tctggttcag gttgttcccg atggcagcca gatgccgcag
     1261 caatggcggt gccagtgtcg gcagttttcc tgtgcgtgca accggctccc ccagacagac
     1321 ccgccgcatc cattccgcca gtcgtttacc ctcgcagcgt tccagtaacc gggcgtgctc
     1381 ctcatcggta acccgtatcg tgagcatctt ctcgcgtttc atcggtatca ttccccctaa
     1441 caaatccccc atacaaggag ccttaagacc atgaccaaac aggaaaaaac agcccttaac
     1501 atggctcgct ttatcagaaa ccagacgcta accctgctgg aaaaactcaa cgatctggac
     1561 gcggacgaac aggcagacat ctgcgaatca cttcacgacc acgccgacga actctaccgc
     1621 agttgcctcg cacgcttcgg cgatgacggt gaaagcaact gaaacatgca gctcccggag
     1681 acgttcacag cttgcctgtg agcggatgcc gggagcagac aagcccgcca gggcgcgtca
     1741 gtgcgggtgt cggggcgcag ccctgaccca gttcacagag cgatagcgat gtgtatactg
     1801 gctttcctca ttttgcatcg gtgcagagtg gatgggaagt gcaccatgtc aggagtaaaa
     1861 agccgcacag atgcgtaaaa acagcgcgcc cgataaacaa cacacacgcg gacactggca
     1921 caggcgcgta agtgcacaat gcttgcctct gtgactcttc cgcttcctcg cccactcgct
     1981 cactacgttc ggtcgctggg gctgcggcga gcggtgggat cactctcgtt gtgatgcaga
     2041 tccgtttttt tttttgcact ggagttatca ggagtagcgg tgcacatggg atcgtgcgat
     2101 atgctggatg gtggaaggtc ggagggaacg gcaacaactc acccctccca gtgaagggac
     2161 gggtagtttg ccgctctcga tatgccggac ggagccaagg ccatcgatca cggggcgcgt
     2221 cagtggaaga acccgcgcct gcgtgacgta tgcgtcttcg ccgcaccatc tcacccgtca
     2281 cgccaccaca taccgggaac tgccgccaag gtctaactca accaatctag ccgcgctaca
     2341 tacggttttt gtggcaactc atcaggtgtc cgctgtcgat gaaactctct gcgccggtag
     2401 cggaactctc acgcgtgcgg tcgccgtcgt cacagctccc ccaaccgcaa acgattctcg
     2461 caaatacgca agtatctgca aaaacaggtt tttttcgggg ttcctgggag actgtgctgt
     2521 tgaaaaactc ttgaagaata cagaaggctc atgcaaactg aatgaactgg tttctgttgt
     2581 ggatctcaac agtttccaca ggaacattga ggaggtcaga gacctcaatg aacccgaaac
     2641 agctcgcctt cctggcgagc tgttttcgtt ctacgcccac cgacgaaaga tcgcaagtgc
     2701 aataacgaga tttttcacgt ctttacgctc aaaaagtaaa aaaaccatgt tttcccctgg
     2761 ctgaatcccc aaaaacgcgt gaaatgcgtt ttcaggatgt tgcatagcta tgcacaaata
     2821 cggcacacga tttgtttcgc tcgccccagc cgtcgaacgg gtcaaaatgg caagatttcg
     2881 tcacttctcc ggtcacatct ccattgatgg cccactccga acccgtgccg cgcgttcctg
     2941 tacctcatgt ctgtaccggg cacgctcctg ttcatccctt agctgttgtt cgcgataaat
     3001 aaccgactca agcgatccac ttgcccggct aatctctgca cctgctgact caatatcacg
     3061 acgcgctcgt tcagtatcgc gttgttccgt tgcgtaacct cgtgcatctt ctgcaattcc
     3121 gcgaaggcgc tctcccactc gttcagccgc cgcatatagt cctgttgcaa atgctctaag
     3181 gcgttcagca aatgcccttc cagttccgtc atactcgttt actccctgac cagcctcacg
     3241 gcgttcttgt cgcccaccgt ccaggtgttt tcccctttca ccccgtccgg caacaccaga
     3301 aatttcccgt tctcgccatt gacgaacgtc accccccagg tttttgcctc gagtttcgcc
     3361 agcgtgtttt cct
//
