LOCUS       NZ_CP123022             2088 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli strain 19-7 plasmid p19-7P5, complete sequence.
ACCESSION   NZ_CP123022
VERSION     NZ_CP123022.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34164623
            Assembly: GCF_029854495.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Li,J.
  TITLE     CRISPR-Cas Diversity and Phylogenetic Distribution of Human
            Carbapenem-resistant Escherichia coli Isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2023) Graduate School of Guangzhou Medical
            University, The First Affiliated Hospital of Guangzhou Medical
            University, No. 195, Dongfeng West Road, Yuexiu District, Guangzhou,
            Guangdong 510030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.0.9
            Assembly Name                     :: 19-7_complete genomics
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10X
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029854495.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:38:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,980
            CDSs (total)                      :: 4,860
            Genes (coding)                    :: 4,599
            CDSs (with protein)               :: 4,599
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 98 of 261
            Pseudo Genes (incomplete)         :: 156 of 261
            Pseudo Genes (internal stop)      :: 57 of 261
            Pseudo Genes (multiple problems)  :: 41 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="19-7"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p19-7P5"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2019-04-13"
     gene            complement(414..680)
                     /locus_tag="QEN55_RS24850"
     CDS             complement(414..680)
                     /locus_tag="QEN55_RS24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(684..1649)
                     /locus_tag="QEN55_RS24855"
     CDS             complement(684..1649)
                     /locus_tag="QEN55_RS24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039064478.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1025..1256)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 cccgcataag ttttttaatc ggtggtgtcg tcaatcacta aatcggaacc ccaaagggga
       61 cccccacgat ttagcatttg acgagattaa ctctcggaga tggcatcatg cttttaacgg
      121 actttaaaaa acggtgaacg gatgcggatt ttaatccgtg tctgtgaggc gttgagcaac
      181 gagcaaagcg aggcggtaga aagagagcca gcggcaggct caggacgata atccggggaa
      241 aatgtaccac tcgcagagtg ttaacatttg acatcggatt gtctatcctg cattggtgtc
      301 gagttggcaa ggctgaggat aatgctgagg ataatgcagg atagacagag cactagagat
      361 tgcatgcaat ctcgtaccta tcacctcagc ctgccgctgg ctcttttcca gaactaagaa
      421 tgcgttctta aaaatccaaa cttgataccg tttaaatcta ttttcacaaa aagagttttt
      481 accctctcgt ttgttttctg aatatataaa attaattcat cctcaaaacc ttcgtgcgga
      541 tgaaaaccaa atgtgccaaa acttaatcca tcaataagca aaataaggtt tttaaacctg
      601 tcaggaatca ccgcttcact tcttaaaaaa aataaaccaa tagtcttatt tccattcagg
      661 tattcaatat taatattcat acgttaatcc gatttatcct tctcaggggc gcgtttgtaa
      721 cgccgtttac ctgaatccca gacaaacgcc gtccgcttcc cgtcatcagt gccatcccct
      781 acatcgtcgg caatggcaag atcctcattg gtttctcgat ccaactggag gacgtttttt
      841 agcgctccac cggtcgagat aaaacggcgc ttgtgaagct gccgcgtcag ctcaagaaac
      901 cactcaggat cgtttgccat atcttccggt ttaacggagt atttcagcgt ttcagcaacc
      961 gcgctttgca gttgctcggc aacgttggcc acaacctcac ctgtcttagt ttttactgcc
     1021 cgaatatcga tattcggctc atagttcacc cgcaagcaat cgcgccagag ttctacccaa
     1081 cgttcgtgct taacgtagtt cttcccttta aaccaagaag gttgcaccat cagcagacag
     1141 tgaaaatgcg gatgtgcgct gccatcctta cctcgcgtca cctccgtagc cctgatccac
     1201 ccctgaacag gtgatagctc ctttcgcttt tccattcgct taaacgccgc attcattgct
     1261 gtaaggactg ttccaagttc acctatctcg cagttcctga cagttaacgt cagaaacaac
     1321 catcgggaag acgggtaatc cacaacgatt ttcggtagag cctgataaaa acgggcttgc
     1381 cacatgaggg ttcttctcca ctggcagaca gggcaatgac ggacacggca aaattgcgca
     1441 ctacgaagcc gtaggcgcgt ttctccggtt tccatgatgg tagaccaacc aaaacgtaac
     1501 aaaccgctac aggaggccat acggagtgca tagcgctcga attcagcagc agtctggtag
     1561 atacccccga cagaatcggt aagcgtgcgc tgggtatccc aaactgcatc acgggggctg
     1621 tagtccgaaa ggaatttatc ttctgacatt aagcctgctc cgatctgggc aggctgtgat
     1681 ttgatgtggt atattcatca cgtacctgtg cattgcgagc cagtacatgt caccagagtt
     1741 tgccgctgag gtgacacatt cgaaatgttg gggagtgttg acgcactccc caacgccgat
     1801 taaaaacgcc tcaattctgc tccattttct ttgacctgtc aaaacccact caaagcctcg
     1861 tcattactgg gctaaaccac tatttcatac ccgccattta tcccttaagt ataaatacaa
     1921 ggatcacgcg cgagaattgc gcaaaaaata accacaattt taacccccca aaacaagcac
     1981 cccgaacggc ttaccccaac cttttccaca cacccgatca ccaaaacagg ggataaaaac
     2041 aaaaccctgt cttacaggtg gatggcctca cttcgtgaaa ccatccac
//
