LOCUS       NZ_CP123021             3376 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli strain 19-7 plasmid p19-7P4, complete sequence.
ACCESSION   NZ_CP123021
VERSION     NZ_CP123021.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34164623
            Assembly: GCF_029854495.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Li,J.
  TITLE     CRISPR-Cas Diversity and Phylogenetic Distribution of Human
            Carbapenem-resistant Escherichia coli Isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2023) Graduate School of Guangzhou Medical
            University, The First Affiliated Hospital of Guangzhou Medical
            University, No. 195, Dongfeng West Road, Yuexiu District, Guangzhou,
            Guangdong 510030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.0.9
            Assembly Name                     :: 19-7_complete genomics
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10X
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029854495.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:38:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,980
            CDSs (total)                      :: 4,860
            Genes (coding)                    :: 4,599
            CDSs (with protein)               :: 4,599
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 98 of 261
            Pseudo Genes (incomplete)         :: 156 of 261
            Pseudo Genes (internal stop)      :: 57 of 261
            Pseudo Genes (multiple problems)  :: 41 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="19-7"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p19-7P4"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2019-04-13"
     gene            complement(join(3222..3376,1..364))
                     /locus_tag="QEN55_RS24825"
     CDS             complement(join(3222..3376,1..364))
                     /locus_tag="QEN55_RS24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_242015765.1"
                     /translation="MKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDAISEHTTSV
                     KKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTALSVTGGIFLGCWGL
                     MEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVLPDGMKGENTWTVGD
                     KNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<252..1094)
                     /locus_tag="QEN55_RS24950"
                     /pseudo=""
     CDS             complement(<252..1094)
                     /locus_tag="QEN55_RS24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1084..1407)
                     /locus_tag="QEN55_RS24835"
     CDS             complement(1084..1407)
                     /locus_tag="QEN55_RS24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1471..1662
                     /locus_tag="QEN55_RS24840"
     CDS             1471..1662
                     /locus_tag="QEN55_RS24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2982..3215)
                     /locus_tag="QEN55_RS24845"
     CDS             complement(2982..3215)
                     /locus_tag="QEN55_RS24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1744..1824)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(4..1094)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.154"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2900..3375)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.146"
                     /coverage="99.0"
                     /evalue="2.78e-62"
ORIGIN
        1 gctctctgat agtcaggata tttttcgcaa tcttgctttc ctgccactcc atcagccccc
       61 agcaccccag gaaaatcccg cccgtgacgc tgagcgcggt cagcgccgga aacagtatcc
      121 gccctttgat cacccgccag agcagttttt tctgctgccg ggacagttcg gcctctgtgg
      181 tgctgaactg catgctcacg gtggtgctca gcgtatccag ttgctttttc acggatgttg
      241 tatgttcgct gatagcgtct ctgattttct gcctgtttaa gctcaatgcc ttgtctacag
      301 actcttcgag cttcctgaac tcgctgttca gcatgttctc ggtagaagcg gctcgctctt
      361 tcagtttctt ctcgaagtct gttcccattt gtaaaagatt gctcatacag cgcccccttt
      421 agcctgagat tccgcccccc ctccgggtcg gcaatactga tgctgctttt ggttgtcctc
      481 accacctcaa aacctgccgc tgtaagcgcc tctgtgacat cctgacgcgt ttttattgct
      541 ccggcatggt aaagagcctc aagaccccgt gtaatcgctt ctgcggcctc ctgtttcgct
      601 tttggcagat tatttggcgt gacaagcgtc cgtctgttct ccggtgcgtt cgggtcacgc
      661 agcccgtaat gatgattaac cagcgtctgc caggcattta tcctcggcct gtccgcacgg
      721 tcgtagtacg gctggaggcg cttaccgctc tgtagctcca tgttcgggat gacaaaattc
      781 agttcgagcc gccccttatc ctggtgttcc acccagagaa tgctgtactg gtctttttcg
      841 agaccgggca taagaacacg ctcaaagctc gtcatcactc tttcgcgttc acccggtggt
      901 agctccttct ccgcgaacga cagaacgcct gacgtgtatt tcttcgaaaa tggcgtggaa
      961 tcaatgagtt cccgcacttc ttccgggtta ccccggagca ccgttgcgcc ttcgcggtta
     1021 cgctcccgcc ccagcaggta atcgaccggg ccacttccac cgcctttccc cctggcatga
     1081 aatttaacta tcatcccgta ccccctgttc cctgacagcc tgccgcaact ggcgcaattc
     1141 accctcgatg gccatcagtg cggccaccac atgaacccgg tcaatcgaag accactggcc
     1201 gctgttcacc ttccgggctg tctggttcag gttgttcccg atggcagcca gatgccgcag
     1261 caatggcggt gccagtgtcg gcagttttcc tgtgcgtgca accggctccc ccagacagac
     1321 ccgccgcatc cattccgcca gtcgtttacc ctcgcagcgt tccagtaacc gggcgtgctc
     1381 ctcatcggta acccgtatcg tgagcatctt ctcgcgtttc atcggtatca ttccccctaa
     1441 caaatccccc atacaaggag ccttaagacc atgaccaaac aggaaaaaac agcccttaac
     1501 atggctcgct ttatcagaag ccagacgcta accctgctgg aaaaactcaa cgatctggac
     1561 gcggacgaac aggcagacat ctgcgaatca cttcacgacc acgccgacga actctaccgc
     1621 agttgcctcg cacgcttcgg cgatgacggt gaaagcaact gaaacatgca gctcccggag
     1681 acgttcacag cttgcctgtg agcggatgcc gggagcagac aagcccgcca gggcgcgtca
     1741 gtgcgggtgt cggggcgcag ccctgaccca gttcacagag cgatagcgat gtgtatactg
     1801 gcttacctca ttttgcatcg gtgcagagtg gatgggaagt gcaccatgtc aggagtaaaa
     1861 agccgcacag atgcgtaaaa acagcgcgcc cgataaacaa cacacacgcg gacactggca
     1921 caggcgcgta agtgcacaat gcttgcctct gtgactcttc cgcttcctcg cccactcgct
     1981 cactacgttc ggtcgctggg gctgcggcga gcggtgggat cactctcgtt gtgatgcaga
     2041 tccgtttttt ttttttttgc actggagtta tcaggagtag cggtgcacat gggatcgtgc
     2101 gatatgctgg atggtggaag gtcggaggga acggcaacaa ctcacccctc ccagtgaagg
     2161 gacgggtagt ttgccgctct cgatatgccg gacggagcca aggccatcga tcacggggcg
     2221 cgtcagtgga agaacccgcg cctgcgtgac gtatgcgtct tcgccgcacc atctcacccg
     2281 tcacgccacc acataccggg aactgccgcc aaggtctaac tcaaccaatc tagccgcgct
     2341 acatacggtt tttgtggcaa ctcatcaggt gtccgctgtc gatgaaactc tctgcgccgg
     2401 tagcggaact ctcacgcgtg cggtcgccgt cgtcacagct cccccaaccg caaacgattc
     2461 tcacaaatac gcaagtatct gcaaaaacag gtttttttcg gggttcctgg gagactgtgc
     2521 tgttgaaaaa ttcttgaaga atacagaagg ctcatgcaaa ctgaatgaac tggtttctgt
     2581 tgtggatctc aacagtttcc acaggaacat tgaggaggtc agagacctca atgaacccga
     2641 aacagctcgc cttcctggcg agctgttttc gttctacgcc cgccgacgaa agatcgcaag
     2701 tgcaataacg agatttttca cgtctttacg ctcaaaaagt aaaaaaacca tgttttcccc
     2761 tggctgaatc cccaaaaacg cgtgaaatgc gttttcagga tgttgcatag ctatgcacaa
     2821 atacggcaca cgatttgttt cgctcgcccc agccgtcgaa cgggtcaaaa tggcaagatt
     2881 tcgtcacttc cccggtcaca tctccattga tggcccactc cgaacccgtg ccgcgcgttc
     2941 ctgtacctca tgtctgtacc gggcacgctc ctgttcatcc cttagctgtt gttcgcgata
     3001 aataaccgac tcaagcgatc cacttgcccg gctaatctct gcacctgctg actcaatatc
     3061 acgacgcgct cgttcagtat cgcgttgttc cgttgcgtaa cctcgtgcat cttctgcaat
     3121 tccgcgaagg cgctctccca ctcgttcagc cgccgcatat agtcctgttg caaatgctct
     3181 aaggcgttca gcaaatgccc ttccagttcc gtcatactcg tttactccct gaccagcctc
     3241 acggcgttct tgtcgcccac cgtccaggtg ttttcccctt tcatcccgtc cggcaacacc
     3301 agaaatttcc cgttctcgcc attgacgaac gtcacccccc aggtttttgc ctcgagtttc
     3361 gccagcgtgt tttcct
//
