LOCUS       NZ_CP123016             2088 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli strain 18-4 plasmid p18-4P3, complete sequence.
ACCESSION   NZ_CP123016
VERSION     NZ_CP123016.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34164601
            Assembly: GCF_029854475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Li,J.
  TITLE     CRISPR-Cas Diversity and Phylogenetic Distribution of Human
            Carbapenem-resistant Escherichia coli Isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2023) Graduate School of Guangzhou Medical
            University, The First Affiliated Hospital of Guangzhou Medical
            University, No. 195, Dongfeng West Road, Yuexiu District, Guangzhou,
            Guangdong 510030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.0.9
            Assembly Name                     :: 18-4_complete genomics
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10X
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029854475.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:36:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,831
            CDSs (total)                      :: 4,714
            Genes (coding)                    :: 4,453
            CDSs (with protein)               :: 4,453
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 90 of 261
            Pseudo Genes (incomplete)         :: 164 of 261
            Pseudo Genes (internal stop)      :: 54 of 261
            Pseudo Genes (multiple problems)  :: 38 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18-4"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p18-4P3"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2018-04-13"
     gene            complement(502..768)
                     /locus_tag="QEN56_RS24115"
     CDS             complement(502..768)
                     /locus_tag="QEN56_RS24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(772..1737)
                     /locus_tag="QEN56_RS24120"
     CDS             complement(772..1737)
                     /locus_tag="QEN56_RS24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039064478.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1113..1344)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 cttttccaca cacccgatca ccaaaacagg ggataaaaac aaaaccctgt cttacaggtg
       61 gatggcctca cttcgtgaaa ccatccaccc cgcataagtt ttttaatcgg tggtgtcgtc
      121 aatcactaaa tcggaacccc aaaggggacc cccacgattt agcatttgac gagattaact
      181 ctcggagatg gcatcatgct tttaacggac tttaaaaaac ggtgaacgga tgcggatttt
      241 aatccgtgtc tgtgaggcgt tgagcaacga gcaaagcgag gcggtagaaa gagagccagc
      301 ggcaggctca ggacgataat ccggggaaaa tgtaccactc gcagagtgtt aacatttgac
      361 atcggattgt ctatcctgca ttggtgtcga gttggcaagg ctgaggataa tgctgaggat
      421 aatgcaggat agacagagca ctagagattg catgcaatct cgtacctatc acctcagcct
      481 gccgctggct cttttccaga actaagaatg cgttcttaaa aatccaaact tgataccgtt
      541 taaatctatt ttcacaaaaa gagtttttac cctctcgttt gttttctgaa tatataaaat
      601 taattcatcc tcaaaacctt cgtgcggatg aaaaccaaat gtgccaaaac ttaatccatc
      661 aataagcaaa ataaggtttt taaacctgtc aggaatcacc gcttcacttc ttaaaaaaaa
      721 taaaccaata gtcttatttc cattcaggta ttcaatatta atattcatac gttaatccga
      781 tttatccttc tcaggggcgc gtttgtaacg ccgtttacct gaatcccaga caaacgccgt
      841 ccgcttcccg tcatcagtgc catcccctac atcgtcggca atggcaagat cctcattggt
      901 ttctcgatcc aactggagga cgttttttag cgctccaccg gtcgagataa aacggcgctt
      961 gtgaagctgc cgcgtcagct caagaaacca ctcaggatcg tttgccatat cttccggttt
     1021 aacggagtat ttcagcgttt cagcaaccgc gctttgcagt tgctcggcaa cgttggccac
     1081 aacctcacct gtcttagttt ttactgcccg aatatcgata ttcggctcat agttcacccg
     1141 caagcaatcg cgccagagtt ctacccaacg ttcgtgctta acgtagttct tccctttaaa
     1201 ccaagaaggt tgcaccatca gcagacagtg aaaatgcgga tgtgcgctgc catccttacc
     1261 tcgcgtcacc tccgtagccc tgatccaccc ctgaacaggt gatagctcct ttcgcttttc
     1321 cattcgctta aacgccgcat tcattgctgt aaggactgtt ccaagttcac ctatctcgca
     1381 gttcctgaca gttaacgtca gaaacaacca tcgggaagac gggtaatcca caacgatttt
     1441 cggtagagcc tgataaaaac gggcttgcca catgagggtt cttctccact ggcagacagg
     1501 gcaatgacgg acacggcaaa attgcgcact acgaagccgt aggcgcgttt ctccggtttc
     1561 catgatggta gaccaaccaa aacgtaacaa accgctacag gaggccatac ggagtgcata
     1621 gcgctcgaat tcagcagcag tctggtagat acccccgaca gaatcggtaa gcgtgcgctg
     1681 ggtatcccaa actgcatcac gggggctgta gtccgaaagg aatttatctt ctgacattaa
     1741 gcctgctccg atctgggcag gctgtgattt gatgtggtat attcatcacg tacctgtgca
     1801 ttgcgagcca gtacatgtca ccagagtttg ccgctgaggt gacacattcg aaatgttggg
     1861 gagtgttgac gcactcccca acgccgatta aaaacgcctc aattctgctc cattttcttt
     1921 gacctgtcaa aacccactca aagcctcgtc attactgggc taaaccacta tttcataccc
     1981 gccatttatc ccttaagtat aaatacaagg atcacgcgcg agaattgcgc aaaaaataac
     2041 cacaatttta accccccaaa acaagcaccc cgaacggctt accccaac
//
