LOCUS       NZ_CP123015             3376 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli strain 18-4 plasmid p18-4P2, complete sequence.
ACCESSION   NZ_CP123015
VERSION     NZ_CP123015.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34164601
            Assembly: GCF_029854475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Li,J.
  TITLE     CRISPR-Cas Diversity and Phylogenetic Distribution of Human
            Carbapenem-resistant Escherichia coli Isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2023) Graduate School of Guangzhou Medical
            University, The First Affiliated Hospital of Guangzhou Medical
            University, No. 195, Dongfeng West Road, Yuexiu District, Guangzhou,
            Guangdong 510030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.0.9
            Assembly Name                     :: 18-4_complete genomics
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10X
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029854475.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:36:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,831
            CDSs (total)                      :: 4,714
            Genes (coding)                    :: 4,453
            CDSs (with protein)               :: 4,453
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 90 of 261
            Pseudo Genes (incomplete)         :: 164 of 261
            Pseudo Genes (internal stop)      :: 54 of 261
            Pseudo Genes (multiple problems)  :: 38 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP123015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18-4"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p18-4P2"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2018-04-13"
     gene            complement(417..608)
                     /locus_tag="QEN56_RS24090"
     CDS             complement(417..608)
                     /locus_tag="QEN56_RS24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            672..995
                     /locus_tag="QEN56_RS24095"
     CDS             672..995
                     /locus_tag="QEN56_RS24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1204..>1641
                     /locus_tag="QEN56_RS24100"
                     /pseudo=""
     CDS             1204..>1641
                     /locus_tag="QEN56_RS24100"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1673..2233
                     /locus_tag="QEN56_RS24105"
     CDS             1673..2233
                     /locus_tag="QEN56_RS24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067486.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGMKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2240..2473
                     /locus_tag="QEN56_RS24110"
     CDS             2240..2473
                     /locus_tag="QEN56_RS24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          253..336
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.059"
                     /coverage="95.0"
                     /evalue="8.83e-18"
     Typing          986..2556
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.439"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actcctgata actccagtgc aaaaaaaaaa aaaacggatc tgcatcacaa cgagagtgat
       61 cccaccgctc gccgcagccc cagcgaccga acgtagtgag cgagtgggcg aggaagcgga
      121 agagtcacag aggcaagcat tgtgcactta cgcgcctgtg ccagtgtccg cgtgtgtgtt
      181 gtttatcggg cgcgctgttt ttacgcatct gtgcggcttt ttactcctga catggtgcac
      241 ttcccatcca ctctgcaccg atgcaaaatg aggtaagcca gtatacacat cgctatcgct
      301 ctgtgaactg ggtcagggct gcgccccgac acccgcactg acgcgccctg gcgggcttgt
      361 ctgctcccgg catccgctca caggcaagct gtgaacgtct ccgggagctg catgtttcag
      421 ttgctttcac cgtcatcgcc gaagcgtgcg aggcaactgc ggtagagttc gtcggcgtgg
      481 tcgtgaagtg attcgcagat gtctgcctgt tcgtccgcgt ccagatcgtt gagtttttcc
      541 agcagggtta gcgtctggct tctgataaag cgagccatgt taagggctgt tttttcctgt
      601 ttggtcatgg tcttaaggct ccttgtatgg gggatttgtt agggggaatg ataccgatga
      661 aacgcgagaa gatgctcacg atacgggtta ccgatgagga gcacgcccgg ttactggaac
      721 gctgcgaggg taaacgactg gcggaatgga tgcggcgggt ctgtctgggg gagccggttg
      781 cacgcacagg aaaactgccg acactggcac cgccattgct gcggcatctg gctgccatcg
      841 ggaacaacct gaaccagaca gcccggaagg tgaacagcgg ccagtggtct tcgattgacc
      901 gggttcatgt ggtggccgca ctgatggcca tcgagggtga attgcgccag ttgcggcagg
      961 ctgtcaggga acagggggta cgggatgata gttaaatttc atgccagggg gaaaggcggt
     1021 ggaagtggcc cggtcgatta cctgctgggg cgggagcgta accgcgaagg cgcaacggtg
     1081 ctccggggta acccggaaga agtgcgggaa ctcattgatt ccacgccatt ttcgaagaaa
     1141 tacacgtcag gcgttctgtc gttcgcggag aaggagctac caccgggtga acgcgaaaga
     1201 gtgatgacga gctttgagcg tgttcttatg cccggtctcg aaaaagacca gtacagcatt
     1261 ctctgggtgg aacaccagga taaggggcgg ctcgaactga attttgtcat cccgaacatg
     1321 gagctacaga gcggtaagcg cctccagccg tactacgacc gtgcggacag gccgaggata
     1381 aatgcctggc agacgctggt taatcatcat tacgggctgc gtgacccgaa cgcaccggag
     1441 aacagacgga cgcttgtcac gccaaataat ctgccaaaag cgaaacagga ggccgcagaa
     1501 gcgattacac ggggtcttga ggctctttac catgccggag caataaaaac gcgtcaggat
     1561 gtcacagagg cgcttacagc ggcaggtttt gaggtggtga ggacaaccaa aagcagcatc
     1621 agtattgccg acccggaggg ggggcggaat ctcaggctaa agggggcgct gtatgagcaa
     1681 tcttttacaa atgggaacag acttcgagaa gaaactgaaa gagcgagccg cttctaccga
     1741 gaacatgctg aacagcgagt tcaggaagct cgaagagtct gtagacaagg cattgagctt
     1801 aaacaggcag aaaatcagag acgctatcag cgaacataca acatccgtga aaaagcaact
     1861 ggatacgctg agcaccaccg tgagcatgca gttcagcacc acagaggccg aactgtcccg
     1921 gcagcagaaa aaactgctct ggcgggtgat caaagggcgg atactgtttc cggcgctgac
     1981 cgcgctcagc gtcacgggcg ggattttcct ggggtgctgg gggctgatgg agtggcagga
     2041 aagcaagatt gcgaaaaata tcctgactat cagagagcag gaaaacacgc tggcgaaact
     2101 cgaggcaaaa acctgggggg tgacgttcgt caatggcgag aacgggaaat ttctggtgtt
     2161 gccggacggg atgaaagggg aaaacacctg gacggtgggc gacaagaacg ccgtgaggct
     2221 ggtcagggag taaacgagta tgacggaact ggaagggcat ttgctgaacg ccttagagca
     2281 tttgcaacag gactatatgc ggcggctgaa cgagtgggag agcgccttcg cggaattgca
     2341 gaagatgcac gaggttacgc aacggaacaa cgcgatactg aacgagcgcg tcgtgatatt
     2401 gagtcagcag gtgcagagat tagccgggca agtggatcgc ttgagtcggt tatttatcgc
     2461 gaacaacagc taagggatga acaggagcgt gcccggtaca gacatgaggt acaggaacgc
     2521 gcggcacggg ttcggagtgg gccatcaatg gagatgtgac cggggaagtg acgaaatctt
     2581 gccattttga cccgttcgac ggctggggcg agcgaaacaa atcgtgtgcc gtatttgtgc
     2641 atagctatgc aacatcctga aaacgcattt cacgcgtttt tggggattca gccaggggaa
     2701 aacatggttt ttttactttt tgagcgtaaa gacgtgaaaa atctcgttat tgcacttgcg
     2761 atctttcgtc ggcgggcgta gaacgaaaac agctcgccag gaaggcgagc tgtttcgggt
     2821 tcattgaggt ctctgacctc ctcaatgttc ctgtggaaac tgttgagatc cacaacagaa
     2881 accagttcat tcagtttgca tgagccttct gtattcttca agaatttttc aacagcacag
     2941 tctcccagga accccgaaaa aaacctgttt ttgcagatac ttgcgtattt gtgagaatcg
     3001 tttgcggttg ggggagctgt gacgacggcg accgcacgcg tgagagttcc gctaccggcg
     3061 cagagagttt catcgacagc ggacacctga tgagttgcca caaaaaccgt atgtagcgcg
     3121 gctagattgg ttgagttaga ccttggcggc agttcccggt atgtggtggc gtgacgggtg
     3181 agatggtgcg gcgaagacgc atacgtcacg caggcgcggg ttcttccact gacgcgcccc
     3241 gtgatcgatg gccttggctc cgtccggcat atcgagagcg gcaaactacc cgtcccttca
     3301 ctgggagggg tgagttgttg ccgttccctc cgaccttcca ccatccagca tatcgcacga
     3361 tcccatgtgc accgct
//
