LOCUS       NZ_CP122937             6646 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Escherichia coli strain ETEC1702 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP122937
VERSION     NZ_CP122937.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549585
            Assembly: GCF_030012765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 131x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030012765.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:03:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,103
            CDSs (total)                      :: 4,982
            Genes (coding)                    :: 4,631
            CDSs (with protein)               :: 4,631
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 351
            CDSs (without protein)            :: 351
            Pseudo Genes (ambiguous residues) :: 0 of 351
            Pseudo Genes (frameshifted)       :: 166 of 351
            Pseudo Genes (incomplete)         :: 195 of 351
            Pseudo Genes (internal stop)      :: 51 of 351
            Pseudo Genes (multiple problems)  :: 54 of 351
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122937.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1702"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="Belgium"
                     /collection_date="2012"
                     /collected_by="IMM_DI04"
     gene            join(6334..6646,1..11)
                     /locus_tag="QDX05_RS25445"
                     /old_locus_tag="QDX05_25440"
     CDS             join(6334..6646,1..11)
                     /locus_tag="QDX05_RS25445"
                     /old_locus_tag="QDX05_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            220..>651
                     /locus_tag="QDX05_RS25450"
                     /old_locus_tag="QDX05_25445"
                     /pseudo=""
     CDS             220..>651
                     /locus_tag="QDX05_RS25450"
                     /old_locus_tag="QDX05_25445"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1207
                     /locus_tag="QDX05_RS25455"
                     /old_locus_tag="QDX05_25450"
     CDS             689..1207
                     /locus_tag="QDX05_RS25455"
                     /old_locus_tag="QDX05_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            1214..1447
                     /locus_tag="QDX05_RS25460"
                     /old_locus_tag="QDX05_25455"
     CDS             1214..1447
                     /locus_tag="QDX05_RS25460"
                     /old_locus_tag="QDX05_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1765..2184)
                     /locus_tag="QDX05_RS25465"
                     /old_locus_tag="QDX05_25460"
     CDS             complement(1765..2184)
                     /locus_tag="QDX05_RS25465"
                     /old_locus_tag="QDX05_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2265..2702)
                     /locus_tag="QDX05_RS25470"
                     /old_locus_tag="QDX05_25465"
     CDS             complement(2265..2702)
                     /locus_tag="QDX05_RS25470"
                     /old_locus_tag="QDX05_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2962..4530
                     /gene="cea"
                     /locus_tag="QDX05_RS25475"
                     /old_locus_tag="QDX05_25470"
     CDS             2962..4530
                     /gene="cea"
                     /locus_tag="QDX05_RS25475"
                     /old_locus_tag="QDX05_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(4527..4868)
                     /locus_tag="QDX05_RS25480"
                     /old_locus_tag="QDX05_25475"
     CDS             complement(4527..4868)
                     /locus_tag="QDX05_RS25480"
                     /old_locus_tag="QDX05_25475"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058759.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            4916..5053
                     /locus_tag="QDX05_RS25485"
                     /old_locus_tag="QDX05_25480"
     CDS             4916..5053
                     /locus_tag="QDX05_RS25485"
                     /old_locus_tag="QDX05_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(5251..5409)
                     /locus_tag="QDX05_RS25570"
     CDS             complement(5251..5409)
                     /locus_tag="QDX05_RS25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(6079..6270)
                     /locus_tag="QDX05_RS25490"
                     /old_locus_tag="QDX05_25485"
     CDS             complement(6079..6270)
                     /locus_tag="QDX05_RS25490"
                     /old_locus_tag="QDX05_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          5907..5994
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(5338..5893)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..1551
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6305..6645
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.368"
                     /coverage="97.0"
                     /evalue="3.24e-66"
ORIGIN
        1 atgatagtta aatttcatgc caggggaaaa ggtggtggca gtggtccggt tgattacctg
       61 ctggggaggg agcgtaaccg cgaaggcgca acggtgcttc agggtaatcc ggaagaagtc
      121 cgggaactca tcgatgccac gccatttgcg aagaaataca cgtccggtgt tctgtcgttc
      181 gcggagaagg agctgccgcc gggaggacgt gaaaaagtga tggcgagctt tgagcgtgta
      241 ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt gggtggagca ccaggacaag
      301 ggacggcttg agctgaattt tgtcattccg aacatggagc tacagaccgg aaaacgcctc
      361 cagccgtact acgaccgcgc agacaggcct agaattgatg cctggcagac gctggtgaat
      421 caccattacg ggctgcatga cccgaacgcc ccggagaacc gcaggacgct gacactccct
      481 gataacctgc ctgaaacgaa acaggcgctt gctgagggcg tcacgcgagg tatagatgca
      541 ctttaccatg ccggagagat aaaaggccgt caggatgtga ttcaggcgct cactgaggcg
      601 gggctggaag tggtcagggt gacgcgaagc agtatcagca ttgcagatcc gaacggcggg
      661 aagaatatca ggctgaaagg agcattttat gagcaatctt ttgcagacgg gcgcggagtt
      721 cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaaca acgagttcag
      781 gaggctcggc gaatctgtaa gcgaggctgt gacatcaaac gagacgaaaa tcagagacgc
      841 tatagccctg ttcacagcct cgacagagga atcgctggaa aaacaccggg aaggggtgaa
      901 agaggcgatg atgcagcaca ggagggacgt gttaaagctg gcagggaata cgggcatgat
      961 gttactgggg atagtctttc tcctgtttac cgcgagtggc gggacgctct ggtatcttgg
     1021 agggaggata caggcgaacc tggaagaaat caggaagcag gaagagacat tgcagaaact
     1081 gaacgcgaag acatggggcg tggagtttgt gcaggacggg aacaggaaat tccttgtcct
     1141 tccgtacggg aaatcagcgg aggtgattcc ctttcagggg aaagagtggg tacatctgaa
     1201 ggagtgacac agagtgacag agctggaaac acatttgctg aacgccttag agcagctgca
     1261 acaggactat atgcagcggc tgagcgaatg ggagagcgcc ttcgtggaat tgcagaagat
     1321 gttttcgctt acgcaacggg acaacgcgat gctgaacgag cgggtcatgc agttgagtca
     1381 gcaggtgcag cacttgagcg agcagacaga acgcttgagc cagttataca gcgagaactg
     1441 gagataagag aggaacggct gatacaggag cgcgaacatg tgttatccct ggaacgggag
     1501 cgtcagccgg aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat
     1561 gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc tctggttgca
     1621 taggtattca tacggttaaa atttatcagg cgcgatcgcg gcagtttttc gggtggtttg
     1681 ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca
     1741 attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc
     1801 agaaatcgtg tcagccagca gcctggattt tttcggggta aggtctttgc tgcggtaatc
     1861 agtacccgca aattgagttg tgccccggag tgagtttaaa aattcctggc tgtgaaactg
     1921 ctgaatgcgt tgttgcacat cactccgtga ttttcctgtc gctttatctt tagctcccat
     1981 atcccagaac tgccgaaaaa aaggcaccat cattcgtgtt gtgtcggctt tttgggtgga
     2041 aaagttggga tcaaaaccgt catcagaagc atatacgtaa tgccgggtat gttgttccac
     2101 gctgcccttt ggggtacagg ctgtgagagc tactgttgct gttatcatgg cgataagaca
     2161 aagtttgttt ccggtacgtc tcatgagtgt ctccctacct taaagtattt attctcggtg
     2221 agttattatc cgaagactat tttatcatca gtctctgaca gcgttcagcg accgaacaac
     2281 ctggaccaga atccccgttt cttttccagt tccagtgcct gcctgagctg ggctatctca
     2341 ttttgtagct gttcccgttc tgcttcctgg cgtctgcgat ccatatcctg tgcctgttta
     2401 tcctcaagca tcagcgtcag gcattgtttc agctcattca gctcccggag aatgcgttct
     2461 gtctgctgat catgtggatt ttctgcatgt ccctcactgt gcctttctgg tgtctcattc
     2521 tgctttaatt cgccgtatgc ccggatcagt tcactggttt caaactcccg tcgtccgtcc
     2581 ctgccggtac ggtaactgac aaggcctgcg cgcatatcac ggtaaaactg tgaacgcgat
     2641 ctgcctgtca tttttagtgc gtcccggagt gtgtgccatg ccataaagtg acagtgtccc
     2701 atagatgtct catctcatag tttcagtaaa acataatgag gtctgagaac ggtaatgttt
     2761 gtgctggttt ttgtggcatc gggcgagaat agcgcgtggt gtgaaagact gtttttttga
     2821 tcgttttcac aaaaatggaa gtccacagtc ttgacaggga aaatgcagcg gcgtagcttt
     2881 tatgctgtat ataaaaccag tggttatatg tacagtattt atttttaact tattgtttta
     2941 aaagtcaaag aggattttat aatggaaacc gcggtagcgt actataaaga tggtgttcct
     3001 tatgatgata agggacaggt aattattact cttttgaatg gtactcctga cgggagtggc
     3061 tctggcggcg gaggtggaaa aggaggcagt aaaagtgaaa gttctgcagc tattcatgca
     3121 actgctaaat ggtctactgc tcaattaaag aaaacacagg cagagcaggc tgcccgggca
     3181 aaagctgcag cggaagcaca ggcgaaagca aaggcaaaca gggatgcgct gactcagcgc
     3241 ctgaaggata tcgtgaatga ggctcttcgt cacaatgcct cacgtacgcc ttcagcaaca
     3301 gagcttgctc atgctaataa tgcagctatg caggcggaag cagagcgttt gcgccttgcg
     3361 aaagcagaag aaaaagcccg taaagaagcg gaagcagcag aaaaggcttt tcaggaagca
     3421 gaacaacgac gtaaagagat tgaacgggag aaggctgaaa cagaacgcca gttgaaactg
     3481 gctgaagctg aagagaaacg actggctgca ttgagtgaag aagctaaagc tgttgagatc
     3541 gcccaaaaaa aactttctgc tgcacaatct gaagtggtga aaatggatgg agagattaag
     3601 actctcaatt ctcgtttaag ctccagtatc catgcccgtg atgcagaaat gaaaacgctc
     3661 gctggaaaac gaaatgaact ggctcaggca tccgctaaat ataaagaact ggatgagctg
     3721 gtcaaaaaac tatcaccaag agccaatgat ccgcttcaga accgtccttt ttttgaagca
     3781 accagacgac gggttggggc cggtaagatt agagaagaaa aacaaaaaca ggtaacagca
     3841 tcagaaacac gtattaaccg gataaatgct gatataactc agatccagaa ggctatttct
     3901 caggtcagta ataatcgtaa tgccggtatc gctcgtgttc atgaagctga agaaaatttg
     3961 aaaaaagcac agaataatct ccttaattca cagattaagg atgctgttga tgcaacagtt
     4021 agcttttatc aaacgctgac tgaaaaatat ggtgaaaaat attcgaaaat ggcacaggaa
     4081 cttgctgata agtctaaagg taagaaaatc ggcaatgtga atgaagctct cgctgctttt
     4141 gaaaaataca aggatgtttt aaataagaaa ttcagcaaag ccgatcgtga tgctattttt
     4201 aatgcgttgg catcggtgaa gtatgatgac tgggctaaac atttagatca gtttgccaag
     4261 tacttgaaga ttacggggca tgtttctttt ggatatgatg tggtatctga tatcctaaaa
     4321 attaaggata caggtgactg gaagccacta tttcttacat tagagaagaa agctgcagat
     4381 gcaggggtga gttatgttgt tgctttactt tttagcttgc ttgctggaac tacattaggt
     4441 atttggggta ttgctattgt tacaggaatt ctatgctcct atattgataa gaataaactt
     4501 aatactataa atgaggtgtt agggatttaa ttattcttta ttgatataaa aagtcctagc
     4561 aatccaaatg ggattgctag gaccaaacaa agtagattat atagcataaa taggtttaat
     4621 tttgctacgg gggcgttatt taggtttttt cttctttcga aaaaatcttt ctttatgaag
     4681 ttaaaagcta tgtgttcaat agcatatttt gaatatggac atagaatagt gcttatcact
     4741 attgcatata gcatcttatc tgacacaagg aaataatacc cttcgctgtt ttttgttata
     4801 aggtatatat atataagtgt gcagtacagg ccaaataaaa tattttttat gtagtatctt
     4861 aagctcataa attaaacctc gccatatatt cttttcattt tataaggatc gagttatgag
     4921 gaaaagattt tttgtgggaa tattcgcgat aaacctcctt gttggatgtc aggctaacta
     4981 tatacgtgat gttcagggag ggaccatcgc accatcctcc tcttctaaac tgacggggat
     5041 cgcggttcag tagaaaagat caaaggatct tcttgagatc cttttttctg cgcgtaatct
     5101 gctgcttgca aacaaaaaaa ccaccgctac caacggtggt ttgtttgccg gatcaagagc
     5161 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5221 ttctagtgta gccgtagtcg ggccactact tcaagaactc tgtagcaccg tttgtgccat
     5281 catcgctctg ctaatccggt taccagtggc tgctgccagt ggcgttaagg cgtgccttac
     5341 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5401 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat accaacagcg
     5461 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5521 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5581 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5641 aggggggcgg agcctatgga aaaacgcctg ctacgtggcc ttcttcctgt tcctggtctt
     5701 ttgctcacat gttctttccg gccttatccc ctgattctgt ggataactgt gttaccgttt
     5761 ttgtgtgagt cagtaccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     5821 aggaagcgga aaagcgcctg gacgtgcatt ttctccttac gcatctgtgc ggcatttcac
     5881 acccggcatg gcgtactttt catacaatcc gcactgatgc cgcatggtta agccagtata
     5941 cactccgcta tcgctacgtg actgggtcag ggctgcgccc cgacacccgc taaaacctgc
     6001 tgacgcgccc tgacgggctt gtcagctccc ggcatccgct cacagacaag ctgtgaccgt
     6061 ctccgggagc tgcatgtatc agaggttttc accgtcatcc ccgaaacgtg cgaggcagct
     6121 gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacaa atatcggcct gttcatctgc
     6181 gtccagttcg ttgagcttct ccagcagcgt taatgtctgg cttctgataa agcgggccat
     6241 gttaagggcg gttttttcct gtttagtcac tgatgcctcc gtgtaagggg gatttctgtt
     6301 catggggtaa tgataccgat gaaacgcgag aggatgctca caatacgggt tactgatgat
     6361 gaacatgccc ggttacttga acgctgtgag ggtaaacaac tggcggtatg gatgcggcgg
     6421 gtctgcctgg gggagccggt tgcccgttcc ggaaaactgc cgacactggc accgccgtta
     6481 ctgcgtcagc tggccgccat cggaaataac ctgaatcaga cagcccgtaa ggtgaacagc
     6541 gggcagtggt cttccggtga ccgggttcag gtggtggccg cactgatggc catcggggat
     6601 gagctgcgcc ggctgcgtct ggccgtcagg gaacaggggg cgcggg
//
