LOCUS       NZ_CP122906             6020 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Escherichia coli strain ETEC1706 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP122906
VERSION     NZ_CP122906.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549588
            Assembly: GCF_030012725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 125x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030012725.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 06:03:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,261
            CDSs (total)                      :: 6,130
            Genes (coding)                    :: 5,570
            CDSs (with protein)               :: 5,570
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 560
            CDSs (without protein)            :: 560
            Pseudo Genes (ambiguous residues) :: 0 of 560
            Pseudo Genes (frameshifted)       :: 268 of 560
            Pseudo Genes (incomplete)         :: 343 of 560
            Pseudo Genes (internal stop)      :: 69 of 560
            Pseudo Genes (multiple problems)  :: 104 of 560
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122906.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1706"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="Belgium"
                     /collection_date="2020"
                     /collected_by="IMM_DI04"
     gene            join(5768..6020,1..8)
                     /locus_tag="QDX08_RS24550"
                     /old_locus_tag="QDX08_24550"
     CDS             join(5768..6020,1..8)
                     /locus_tag="QDX08_RS24550"
                     /old_locus_tag="QDX08_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="WP_000864323.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEILA
                     AAFVIFYSGVLLFILYFCFSALTDLQKPDDERKSDERNEDE"
                     /name="conjugal transfer protein TrbE"
     gene            1..744
                     /gene="traF"
                     /locus_tag="QDX08_RS24555"
                     /old_locus_tag="QDX08_24555"
     CDS             1..744
                     /gene="traF"
                     /locus_tag="QDX08_RS24555"
                     /old_locus_tag="QDX08_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     protein TraF"
                     /protein_id="WP_001030376.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAAP
                     RQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKAML
                     AHPELDYNLQYSHYNGTVRNQLAADQVQQRQAIAKLAEHYGIMFFYRGQDPIDGQLAQV
                     INGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGSVRPL
                     SYGFISQDDLAKQFLNVSEDFKPNF"
                     /name="traF"
     gene            760..1107
                     /gene="trbA"
                     /locus_tag="QDX08_RS24560"
                     /old_locus_tag="QDX08_24560"
     CDS             760..1107
                     /gene="trbA"
                     /locus_tag="QDX08_RS24560"
                     /old_locus_tag="QDX08_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="WP_001287893.1"
                     /translation="MSEDYLKLFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVIN
                     IACLYVLTASLWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDGFRRIFICIMLSSVWSGV
                     MWFFIRGLMTG"
                     /name="trbA"
     gene            1226..1509
                     /gene="traQ"
                     /locus_tag="QDX08_RS24565"
                     /old_locus_tag="QDX08_24565"
                     /pseudo=""
     CDS             1226..1509
                     /gene="traQ"
                     /locus_tag="QDX08_RS24565"
                     /old_locus_tag="QDX08_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /name="traQ"
     gene            1496..>2029
                     /gene="trbB"
                     /locus_tag="QDX08_RS24570"
                     /old_locus_tag="QDX08_24570"
                     /pseudo=""
     CDS             1496..>2029
                     /gene="trbB"
                     /locus_tag="QDX08_RS24570"
                     /old_locus_tag="QDX08_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     thiol-disulfide isomerase TrbB"
                     /name="trbB"
     gene            2030..2344
                     /gene="trbJ"
                     /locus_tag="QDX08_RS24575"
                     /old_locus_tag="QDX08_24575"
     CDS             2030..2344
                     /gene="trbJ"
                     /locus_tag="QDX08_RS24575"
                     /old_locus_tag="QDX08_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="WP_001384255.1"
                     /translation="MRNKQVVLLIAGISGIATGIIVSLNIPFIRQGLFYPASPVEIVVS
                     LCLTFSVSVVFFVGAIVGWILVSEIYYSRMTGLNESSVISEETYNDSKQKESGIQNQKN
                     "
                     /name="trbJ"
     gene            2298..2690
                     /gene="trbF"
                     /locus_tag="QDX08_RS24580"
                     /old_locus_tag="QDX08_24580"
     CDS             2298..2690
                     /gene="trbF"
                     /locus_tag="QDX08_RS24580"
                     /old_locus_tag="QDX08_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="WP_062873756.1"
                     /translation="MTANKKNQEFKIRKIRRNIEYSFRDSDRYFDLFIVFLVAGIVLWA
                     VMHVIFDVCIDSWMADPKLLNFQYMWNVLMKVIPFTLWALAAGFLVTFFLSPMCELIFG
                     NIMIFLLKRRMRRENTLREGKNDATH"
                     /name="trbF"
     gene            2677..>3259
                     /locus_tag="QDX08_RS24585"
                     /old_locus_tag="QDX08_24585"
                     /pseudo=""
     CDS             2677..>3259
                     /locus_tag="QDX08_RS24585"
                     /old_locus_tag="QDX08_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944327.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /name="conjugal transfer protein TraH"
     gene            3340..3936
                     /gene="trbC"
                     /locus_tag="QDX08_RS24590"
                     /old_locus_tag="QDX08_24590"
     CDS             3340..3936
                     /gene="trbC"
                     /locus_tag="QDX08_RS24590"
                     /old_locus_tag="QDX08_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     protein TrbC"
                     /protein_id="WP_282857393.1"
                     /translation="MHITVHASDNVNTPENREFLKQQENSSRQLREKPDHQLKAWAEKQ
                     VLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPEEGLKRMLGETRHYGIP
                     ATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGIRSVPALVVFCSQGYDII
                     RGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
                     /name="trbC"
     gene            3933..5741
                     /gene="traN"
                     /locus_tag="QDX08_RS24595"
                     /old_locus_tag="QDX08_24595"
     CDS             3933..5741
                     /gene="traN"
                     /locus_tag="QDX08_RS24595"
                     /old_locus_tag="QDX08_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="WP_063078212.1"
                     /translation="MKRILPLILALVAGMALADSNSDYRAGSDFAHQIKGQGSSSIQGF
                     KPQESIPGYNANPDETKYYGGVTAGGDSGLKNDGTTEWATGETGKTITESFMNKPKDIL
                     SPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRTARM
                     ELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQKSVPM
                     TITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMKLRLVV
                     RVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSCWAYRDT
                     YVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGKVMVCGGD
                     VFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQAKFCKKAAA
                     GYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGVCLEKKRSYC
                     QFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNN
                     QKIPDSGVLTQKVKEQIADQLKQAGQ"
                     /name="traN"
     Typing          3350..3933
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="96.41"
                     /coverage="92.0"
                     /evalue="6.85e-123"
     Typing          3934..5738
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.502"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaataaag cattactgcc actgttactc tgctgcttta tttttccggc gtcaggaaaa
       61 gatgcaggct ggcagtggta taacgagaaa ataaatccga aggaaaaaga aaataaacca
      121 gtacctgcag ccccccgtca ggagccggac attatgcaga aactggccgc actgcagacg
      181 gcaacaaagc gggcgctgta cgaagctatt ctgtatcccg gcgtggataa ttttgtgaaa
      241 tatttccggc tgcagaatta ctggactcag caggccgggc ttttcaccat gagcgccaaa
      301 aaagccatgc tggcgcatcc cgaactggac tataacctgc agtacagcca ttacaacggc
      361 acggtccgga accagctggc ggcagaccag gtgcagcagc gacaggccat tgcgaaactg
      421 gctgaacact acggcatcat gttcttttac cgggggcagg accccatcga cgggcagctg
      481 gcgcaggtca ttaatggctt cagggatacc tatggtctga gtgttattcc cgtttccgtg
      541 gatggtgtga ttaatccgct gttgccggat tcccggactg accaggggca ggcgcagcgc
      601 ctcggcgtga aatatttccc ggccatgatg ctggttgacc cgaaacaggg cagtgttcgt
      661 ccgttatcat acggttttat ttcgcaggac gacctggcaa aacagttcct gaacgtttct
      721 gaagatttta aacccaattt ttaatcgcgg atttgattta tgagtgaaga ttatttgaaa
      781 ctgtttacag gtgttgttct gttaatattt gtcattattg ccggttattt cttttctgag
      841 cgtaatgaca ggaaaatgtt tctcctgagt tcgctggttt tccttgttat taatatcgcg
      901 tgtttatatg tgctgactgc cagtctctgg tttctgtgtg gtgcaattat gaatcagggc
      961 gcagcaatgg ttgttccaat ggttattggc tcattaccga acgttacgag cttcgacgga
     1021 ttcagaagaa tatttatctg tattatgttg tcatcagtat ggtccggagt gatgtggttt
     1081 tttataaggg ggcttatgac aggctaagtc atataaaacg gtaataaaaa ccatacaaaa
     1141 taatgaggtt gatctgatta atatcacaca aacgcgaata ttacagacat aaatactctc
     1201 atgtaaataa taaccggagt ttaacatgat aagtaaacgc agattctctt taccccggct
     1261 tgatattacg ggaatgtggg tattttccct gggtgtctgg tttcatatcg tcgcccgtct
     1321 tgtttacagc aaaccctgga tggctttttt ctggcggagc tgattgcggc catccttgtg
     1381 ctgttcggcg cataccaggt gctggatgca tggattgccc gtgtcagccg ggaagagcgt
     1441 gaggcactgg aagccaggca acaggccatg atggcagagc agcaggaggg cggacatgtc
     1501 tctcactaaa ttactgccgg tcattttgtt gatgatggcc acaggtgtgc aggcctccac
     1561 ccgggatgaa attgaacgac tctggaatcc gcagggtatg gccgcgcagc ctgcacaacc
     1621 ggcagcagac acgtcagcca ggacagaaaa gccggctccc cgctggttcc gcctcagtaa
     1681 tggcaggcag gttaacctgg ccgactggaa ggtggtgctg tttatgcagg ggcattgccc
     1741 ttactgtcac cagtttgacc cggtactgaa acagctggca cagcagtacg gcttttccgt
     1801 ttttccctac acactggatg gtcaggggga taccgccttt ccggaggcat tgccggtgcc
     1861 accggacgta atgcagacct tcttcccgaa tatcccggtg gccacaccga ccaccttcct
     1921 ggtcaacgtc aacacgcttg aggcattacc gcttttacag ggggcaacgg atgccgccgg
     1981 ttttatggcg cggatggata ccgttctgca gatgtacaga ggaaaaaagg tgcgaaataa
     2041 gcaggtggtg ttactcattg ccggaatatc cggcatcgcg acgggaataa tcgtcagcct
     2101 gaatatcccc tttatccgtc aggggctttt ttatcccgcc agccctgtag aaatagtcgt
     2161 ttcgctgtgt cttacctttt ctgtttctgt tgtgtttttt gtgggggcaa ttgtgggatg
     2221 gattttggtg tctgaaatat attacagccg catgaccggt ctgaatgaat catcagtaat
     2281 atcagaggaa acttataatg acagcaaaca aaaagaatca ggaattcaaa atcagaaaaa
     2341 ttagacggaa tattgagtat agtttcaggg attctgacag atattttgat ttgtttattg
     2401 ttttccttgt tgccggaatt gttttatggg cagttatgca cgttattttc gatgtctgta
     2461 ttgattcatg gatggccgac ccgaaattac ttaatttcca gtatatgtgg aatgttctga
     2521 tgaaagtcat tccgtttacg ttatgggcgc tggcagcagg gtttcttgtt acattttttc
     2581 tgtccccaat gtgcgaatta atattcggaa atatcatgat attcctgctg aaacgccgta
     2641 tgcgccggga gaacacatta agggagggca agaatgatgc cacgcattaa acctcttctg
     2701 gttctttgtg ctgctttact gacggtcaca cccgcagcgt cagcggatgt gaacagcgac
     2761 atgaatcagt tctttaacaa gctgggcttc gcctccaata ccacacagtc gggcgtctgg
     2821 caggggcagg ctgccggcta tgcttacggc ggctccctgt atgcccgtac tcaggtcaaa
     2881 aatgttcagc tgatttccat gacgctgccg gatatcaatg ccggatgtgg cggcatcgac
     2941 gcttatctcg gttcgttcag ttttattaat ggtgagcagc tgcagcgttt tgttaagcag
     3001 attatgagca atgctgccgg ttactttttt gaccttgccc tgcagacaac ggtgccggaa
     3061 atcaaaaccg caaaagactt cctgcagaaa atggcaagcg acattaacag tatgaacctc
     3121 agttcctgcc aggcggcaca ggggattatc ggcgggctct tcccccggac gcaggtgtcc
     3181 cagcagaaaa gtctgtcagg acattgccgg tgagagcaat atttttgctg actgggcgtc
     3241 ttcccggcag ggatgtactg cttggcggga aatctgacag cgcaggatat atcatgaact
     3301 ggaaaacatt tgcgcttttt gccctgacaa gctgcaggaa tgcatatcac tgttcatgcc
     3361 tcagacaatg tgaacactcc tgaaaaccgc gaattcctga agcagcagga aaattcaagc
     3421 agacaactgc gtgaaaaacc tgaccatcag ctgaaggcct gggcggagaa acaggtgctg
     3481 gagaaccccc ttcagcgttc agataaccat ttcctggatg agctggttcg taaacagcag
     3541 gcttcgcagg acgggaaacc ccggcagggg gccctgtatt ttgtgtcgtt ttccattccc
     3601 gaagaggggc tgaaacggat gctgggcgaa acccggcact acggtatacc cgccacactg
     3661 cggggcatgg tgaacaatga cctgaagacc accgctgaag ccgtgctgtc cctggtgaaa
     3721 gacggcgcga ctgatggtgt tcagatcgac ccgacgctgt tttcacagta cggcattcgc
     3781 agtgtaccgg cactggtggt gttctgcagt cagggatacg acatcatccg gggaaacctg
     3841 cgggtcggcc aggcgctgga gaaagtggcc gccacgggtg actgccggca ggtggctcat
     3901 gatttactgg cagggaaagg agattccggg aaatgaaacg tattttacct ctgatactgg
     3961 ctctggttgc cggcatggca ctggctgaca gcaacagtga ttaccgggcc ggctctgatt
     4021 ttgctcacca gatcaaagga cagggaagca gcagtattca gggctttaag ccacaggaga
     4081 gtatccccgg ctataacgcg aatccggacg agacaaaata ttacggtggc gtgactgccg
     4141 gcggggatag cggcctgaaa aatgacggca ccacggaatg ggcgaccggt gaaaccggaa
     4201 aaaccattac agagtcattc atgaacaagc cgaaagacat tctttcaccg gatgcaccgt
     4261 ttatccagac cggcagggat gtggtgaacc gggctgacag cattgtggga aacaccgggc
     4321 agcagtgcag tgcgcaggag attagccgga gtgaatacac gaattacacc tgtgagcggg
     4381 atttacaggt ggagcagtac tgcacacgaa ctgcccgtat ggagctgcag gggagtacca
     4441 catgggaaac ccggacgctg gagtatgaaa tgagtcagct acctgcacgt gaagtgaatg
     4501 gtcagtatgt tgtctccatt acctctcccg ttaccggtga aattgtcgat gcgcattaca
     4561 gctggagtcg tacttacctg cagaagagtg tacctatgac aattacagta ctgggaaccc
     4621 cactgagctg gaatgccaaa tactcagcag atgcctcatt tacaccagta cagaaaacac
     4681 tgactgccgg tgttgctttt acgtcatctc accccgtccg ggtcgggaat acaaaattca
     4741 aacgtcatac ggcaatgaag ctgcgtctgg ttgtcagggt aaaaaaagcc tcgtacaccc
     4801 cgtatgttgt ctggtctgaa agctgcccgt tcagcaagga gctggggaaa ctgacaaaaa
     4861 cagaatgtac ggaggctggc gggaacagga cgctggtgaa agacggtcag tcatacagca
     4921 tgtaccagag ctgctgggca taccgggaca cgtatgtcac acagtcagca gacaagggaa
     4981 cctgtcagac ctacaccgac aatcctgcct gtaccctggt gtcacaccag tgcgcctttt
     5041 actccgaaga aggtgcctgt ctgcatgaat atgccacgta ctcctgtgag tcaaaaacat
     5101 ccgggaaagt gatggtctgt gggggagacg tcttctgtct cgatggcgaa tgcgataagg
     5161 ctcagagcgg gcagagtaat gattttgccg aagccgtttc tcagctggcg gcactggccg
     5221 cagcaggtaa ggatgttgcg gcgctgaacg gggtggatgt ccgtgctttc accggtcagg
     5281 cgaaattttg taagaaggct gcagccggat acagcaactg ctgtaaggac agcggctggg
     5341 gacaggatat cgggctggcc aaatgcagca gtgatgaaaa agccctggct aaagcaaaat
     5401 caaacaaact gaccgtcagt gtcggagagt tctgttcgaa gaaagtgctg ggtgtctgcc
     5461 tggagaaaaa acgcagttac tgtcagtttg attcgaagct ggcgcagata gtccagcagc
     5521 aggggcgcaa cggtcagctg cgtatcagtt ttggcagtgc gaagcatcct gactgtcggg
     5581 ggattacggt tgatgagctg cagaaaattc agttcgacag actggatttc actaacttct
     5641 acgaagacct gatgaataac cagaaaatcc cggacagtgg tgttctgacg cagaaagtga
     5701 aagagcagat tgctgaccag ctgaaacagg caggacagta aggtatggaa aataaccggg
     5761 gaaaatgatg aaagtcatat tcacgtcgaa ccgttttatt gattttctta tccggctttt
     5821 gattacagcg attgtcattt ccccggtaat tatctggtcg tgggatacgg taaaagaaac
     5881 cactgcaggt gagatacttg ctgctgcttt cgttattttt tactccggtg tgctcctgtt
     5941 tattctgtat ttttgttttt ctgccctgac tgacctgcag aaacctgatg atgaaagaaa
     6001 aagtgatgaa aggaatgaag
//
