LOCUS       NZ_CP122808             6827 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Escherichia coli strain ETEC1734 plasmid unnamed7, complete
            sequence.
ACCESSION   NZ_CP122808
VERSION     NZ_CP122808.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549611
            Assembly: GCF_030013675.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030013675.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:21:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,738
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,303
            CDSs (with protein)               :: 5,303
            Genes (RNA)                       :: 134
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 136 of 301
            Pseudo Genes (incomplete)         :: 164 of 301
            Pseudo Genes (internal stop)      :: 52 of 301
            Pseudo Genes (multiple problems)  :: 42 of 301
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122808.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1734"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            1..951
                     /locus_tag="QDX84_RS28045"
                     /old_locus_tag="QDX84_28045"
     CDS             1..951
                     /locus_tag="QDX84_RS28045"
                     /old_locus_tag="QDX84_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_034167667.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            959..1372
                     /locus_tag="QDX84_RS28050"
                     /old_locus_tag="QDX84_28050"
     CDS             959..1372
                     /locus_tag="QDX84_RS28050"
                     /old_locus_tag="QDX84_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229901.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            1619..1936
                     /locus_tag="QDX84_RS28055"
                     /old_locus_tag="QDX84_28055"
     CDS             1619..1936
                     /locus_tag="QDX84_RS28055"
                     /old_locus_tag="QDX84_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_040113353.1"
                     /translation="MFNVILHDDVPNELAGLPPVVRAKMIRLIDKLKTNATALREPDSK
                     PLRDGLFELRTMGTDIARGLYVYQKGRNIYLLRVFIKKTQKTPSGEISMAFERLKEMLN
                     E"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1929..2216
                     /locus_tag="QDX84_RS28060"
                     /old_locus_tag="QDX84_28060"
     CDS             1929..2216
                     /locus_tag="QDX84_RS28060"
                     /old_locus_tag="QDX84_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104592.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_282835527.1"
                     /translation="MSKVKGIVWEHVRNELMSNPEVQAAYEAEERKERLQAMLAEWRHH
                     AGLTRAQVAERMGVTPPTVSRMESNVIKASIETLARYARACGVKHPQITL"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2443..3423
                     /locus_tag="QDX84_RS28065"
                     /old_locus_tag="QDX84_28065"
     CDS             2443..3423
                     /locus_tag="QDX84_RS28065"
                     /old_locus_tag="QDX84_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_000019448.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQNPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            3405..3599
                     /locus_tag="QDX84_RS28760"
     CDS             3405..3599
                     /locus_tag="QDX84_RS28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_349239574.1"
                     /translation="MGEISVKTGNNARNGGKNSLNRLIRCVCGKKIGPDPRNFNQRVNL
                     GRNDLLIRTFPKARSCITI"
                     /name="hypothetical protein"
     gene            complement(3908..4492)
                     /locus_tag="QDX84_RS28070"
                     /old_locus_tag="QDX84_28070"
     CDS             complement(3908..4492)
                     /locus_tag="QDX84_RS28070"
                     /old_locus_tag="QDX84_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_040113348.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(4505..5602)
                     /gene="mobA"
                     /locus_tag="QDX84_RS28075"
                     /old_locus_tag="QDX84_28075"
     CDS             complement(4505..5602)
                     /gene="mobA"
                     /locus_tag="QDX84_RS28075"
                     /old_locus_tag="QDX84_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_282835522.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     NTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQR
                     QQEKEQQRLAEQTRQKPDKGWSFSR"
                     /name="mobA"
     gene            5768..6235
                     /gene="traD"
                     /locus_tag="QDX84_RS28080"
                     /old_locus_tag="QDX84_28080"
     CDS             5768..6235
                     /gene="traD"
                     /locus_tag="QDX84_RS28080"
                     /old_locus_tag="QDX84_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_032235402.1"
                     /translation="MSKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     Typing          776..951
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(4509..5602)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.726"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg
       61 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac
      121 cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca gatgatgaac
      181 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga
      241 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat
      301 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt
      361 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa
      421 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag
      481 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac
      541 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact
      601 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc
      661 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa
      721 atgaatggtg acaaaatacc aacaaccatt acatcagatt cccacctaca taacggacta
      781 agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa
      841 tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg gctcacgcaa
      901 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat
      961 ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa caactgttca
     1021 ccgttacata tcaaagggaa aactgtccat atgcacagat gaaaacggtg taaaaaagat
     1081 agatacatca gagcttttac gagtttttgg tgcattcaaa gctgttcacc atgaacagat
     1141 cgacaatgta acagatgaac agcatgtaac acctaataga acaggtgaaa ccagtaaaac
     1201 aaagcaacta gaacatgaaa ttgaacacct gagacaactt gttacagctc aacagtcaca
     1261 catagacagc ctgaaacagg cgatgctgct tatcgaatca aagctgccga caacacggga
     1321 gccagtgacg cctcccgtgg ggaaaaaatc atggcaattc tggaagaaat agcgctttca
     1381 gccggcaaac cggctgaagc cggatctgcg attctgataa caaactagca acaccagaac
     1441 agcccgtttg cgggcagcaa aacccgtact tttggacgtt ccggcgggtt tttgtggcga
     1501 gtggtgttcg ggcggtgcgc gcaagatcgc ttatgttaaa tacagcagaa aaaccataaa
     1561 attaccctat aacgcaaatt gctttttatg ctaatatttc gcaatggagg gcgaaccagt
     1621 gtttaacgtc atattgcacg atgatgtacc aaatgagctg gctggcttac cgccagtagt
     1681 cagagcgaag atgattcgcc tgattgataa acttaaaacc aacgctactg cattacgaga
     1741 gccagatagc aaaccactcc gggatggatt atttgagtta aggacgatgg ggacggatat
     1801 tgccagaggt ctttatgttt atcagaaagg caggaatatc tatctactta gagtctttat
     1861 taaaaagact cagaaaaccc catcgggtga aatatctatg gcctttgaac gtcttaagga
     1921 gatgctaaat gagtaaagtt aaaggtatcg tttgggagca tgttaggaat gaattgatgt
     1981 ctaatcctga ggttcaagca gcctatgaag cagaggagcg caaggaaaga cttcaagcta
     2041 tgctcgctga atggcgtcat cacgcaggat taacaagagc ccaggtggca gaacgcatgg
     2101 gtgttactcc acctaccgtt tctcgcatgg aatctaatgt gattaaggct agcattgaaa
     2161 cactggctcg atacgcaaga gcatgcggtg ttaagcaccc tcagatcacg ttgtaatttt
     2221 caaggccgct ttaagcagcc ttttgatgcg tgcaagatcg cttatgttga acaaggagtt
     2281 tttttgcaaa aaaccatcat ttcacacata tgagataatc ctaagggata taaataggct
     2341 aattttgccc aacgcgccaa aaatgtaaag ttagggaagg tgcgaacaag ttcctgatat
     2401 gagatcatca tattcatccg gagcgcatcc caaagggaca tcatgagcca tcaactcacc
     2461 ttcgccgata gtgaattcag cactaagcgc cgtcagaccc gaaaagagat tttcctctcc
     2521 cgcatggagc agattctgcc atggcagaat atgaccgctg tcatcgagcc gttttatccc
     2581 aaggcgggca atggccgacg gccctatccg ctggagacca tgctgcgtat tcactgcatg
     2641 cagcattggt acaacctgag cgacggtgcc atggaagatg ccctgtacga aatcgcctcc
     2701 atgcgcctgt ttgcccgatt atccctggat agcgccctgc cggatcgcac cactatcatg
     2761 aatttccgcc acctgctcga gcagcatcaa ctggcccgtc aattgttcaa gaccatcaat
     2821 cgctggctgg ccgaagcagg cgtcatgatg acccaaggca ctttggtgga tgccaccatc
     2881 attgaggcac ccagctctac caagaacaaa gagcagcaac gcgatccgga gatgcatcag
     2941 accaagaaag gcaatcagtg gcactttggc atgaaggccc acattggtgt cgatgccaag
     3001 agtggcctga cccacagcct ggtcaccacc gcggccaacg agcatgacct caatcagctg
     3061 ggtaatctgc ttcatggaga ggagcaattt gtctcagccg atgccggcta ccaaggagcg
     3121 ccacagcgcg aggagctggc cgaggtggat gtggactggc tgatcgccga gcgtcccggc
     3181 aaggtaaaaa ccttgaagca gaatccgcgc aagaacaaaa cggccatcaa catcgaatac
     3241 atgaaagcca gcatccgtgc cagggtggag cacccgtttc gcatcatcaa gcggcagttc
     3301 ggcttcgtga aagccagata caaggggctg ctgaaaaacg ataaccaact ggcgatgtta
     3361 ttcaccctgg ccaacctgtt tcgggtggac caaatgatac gtcagtggga gagatctcag
     3421 taaaaaccgg aaataacgcc agaaatggtg gaaaaaatag cctaaatagg ctgattcgat
     3481 gtgtttgcgg gaaaaaaatc ggcccagatc cgcgaaattt taatcagcga gtcaacttgg
     3541 gaagaaatga cctgcttatt cgcaccttcc ctaaagctcg aagctgtata accatctgac
     3601 ctgcaattcg ttgatgataa agggttagac cccgtgaggc ctatctggct attgtacagg
     3661 atatacgtat ccgcttaacg aacaagaacc tcacgcgcgt ctttcatcag ggcccgaatc
     3721 gatgacgatt catcatggcc gtttatagta ccgcagtctc tcccctttat ttttactgaa
     3781 gagcagacag aaaccataaa taccggcttt gacatgccgg ggaagtcctt atagtacaat
     3841 aattctctat atccaaactg accccctaat tcacgacaac aataacggcg caatgtgcgc
     3901 cgttatgtta ttttttgata atcatccagg tattctcttt atcaccatac gtagctcggg
     3961 tgtccacctt aacgcagggc tttccgtcac aacgcgatat atcagccagc ggggctttct
     4021 tctgccagac cgcttccatc cgttgcattt cagcaatctg gctgtacccg tcattcataa
     4081 accacgtaaa tgccgtcacg caggcagcca tgacgaaaaa tatcgtcagc acgagataaa
     4141 tcgcggattt ccacgtatag cgcgacatct cgcgtcgcat ttcatggatc atcgccttcg
     4201 ccgtatcggt agcctgatta agcgcttctg tcgccggttt ctgctgtgct aatccggctt
     4261 gtttcagctc tttctcaacc tgaatgagcg cggcatgaac agattgcctg acggtttcag
     4321 tcatattacc ggacgcgctg tccagctcac gaatgagcct gctcagcgtg tcactctgct
     4381 gctgtaattg tgctgaggcg tcctgaaact gctcagtcag agaggtaaca cgcttttcca
     4441 gcgcctgatg atgcccgatg agggcagcga tttgtttaat ttcgtcgctc atgaaaaatc
     4501 ctgtctatcg tgaaaatgac cagcctttat ccggcttctg tcgtgtctgt tcggcgagtc
     4561 tctgctgttc tttctcctgc tgacgctgtt tttctgccag tcgctccatt tcctgatgta
     4621 tcccctgaaa ctccgccatt gtctcgttca caagggactg aaggtcgatt tcgtcctgta
     4681 cgtccttcag cgcatcactg accagtgcgt tcagcttctc cggctctttc tcaaaatcga
     4741 aaaccctgcc ggaagtgttt tcctgcgcag gttcaggctt ctgctcttgt tttatcgtca
     4801 ggttatcccg ctcgctgatg gcttcccgta gcgaggtggt cacgtcaatc acactgtcac
     4861 gttcatcacg agactgctcc gcctgccttt cagcgtccct gcgctcaagg atggcctgta
     4921 actgcgcagt atcgaaccgc tgaacctgtc ccgcgccaag atgccgctca ggctcacgct
     4981 caatgccctg agctttgagt gagcgtgcat caacgcggtc ggtatgctga taccgttcaa
     5041 ggtgcttatt ctgtaggtca gcccagcgtt cccgctgggc gacgaggtgc tctttgcgtt
     5101 ctgtcggcgt ttccccgaaa cgtgcctttt tcgcaccgcc gcgctcaggg gctttgctgt
     5161 tagctcgctt aaaatactgc tgcgggtcac ggtcgatacc gtcattcatc cgctcagaga
     5221 acatgatatg cgcgtgcggc tgctcgccgc cggctatcgc cgctttcgga ttatgaatgg
     5281 cgaactgata cgcatgacgg tcgccgattt cctgccggac aaaatcgcgg accagttcaa
     5341 ggcgttgttc gggtttcagc tcacgcggca gggcaatctc gatttcacgg taggtacagc
     5401 cgttggcacg ttcaaacgtg tcagcggctt tccagaactc cgtcggttta tgcgctgccc
     5461 aggcaggcat attgccggac tccttgtgct caaggtcgga gtctttttca cgggcatact
     5521 ttccctcacg cgaaatataa tcggcatggg gagaggcact gcctttcccg ccggttttta
     5581 cgctgaggtg ataggaggcc atcgtgtttt tatcccgctg aaggcgcgca ccgtttctga
     5641 acgaagtgaa gaaacgtcta agtgcgccct gataaataaa agagttatca gggattgtag
     5701 ttgaatttga cctccactgc catcaatgag cataattccc ccgttaaaac tcagagggta
     5761 aacgggcatg agtaaaagcg aaaaacagga acaatgggca gcagaaagag tgcagtatat
     5821 tcgcggctta aagtcgccga atgagcaaca gaaacttatg ctgatactga cggataaagc
     5881 agataaaaca gcacaggata tcaaaacgct gtccctgctg atgaaggctg aacaggcagc
     5941 agagaaagcg caggaagcca gagcgaaagt catgaacctg atacaggcag aaaagcgagc
     6001 cgaagccaga gccgcccgta aagcccgtga ccatgctctg taccagtctg ccggattgct
     6061 tatcctggcg ggtctggttg acagtaagac gggtaagcct gttgatgata ccgctgcctt
     6121 actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg
     6181 gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac
     6241 ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc
     6301 ttgcatgaat ccataaaagg cgcctgtagt gccatttccc cccattcact gccagagccg
     6361 tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag
     6421 acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg
     6481 ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt taatactgcg
     6541 gaactgacta aagtagtgag ttatacacag ggctgggatc tattcttttt atcttttttt
     6601 attctttctt tattctataa attataacca cttgaatata aacaaaaaaa acacacaaag
     6661 gtctagcgga atttacagag ggtctagcag aatttacaag ttttccagca aaggtctagc
     6721 agaatttaca gatacccaca actcaaagga aaaggactag taattatcat tgactagccc
     6781 atctcaattg gtatagtgat taaaatcacc tagaccaatt gagatgt
//
