LOCUS       NZ_CP122708             8475 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli strain ETEC4061 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP122708
VERSION     NZ_CP122708.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549638
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 315x
            Sequencing Technology        :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_030166025.1-RS_2024_06_19
            Annotation Date              :: 06/19/2024 11:46:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 9
            CDSs (total)                 :: 9
            Genes (coding)               :: 9
            CDSs (with protein)          :: 9
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122708.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC4061"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            1..2220
                     /gene="mobQ"
                     /locus_tag="QDX28_RS00005"
                     /old_locus_tag="QDX28_27125"
     CDS             1..2220
                     /gene="mobQ"
                     /locus_tag="QDX28_RS00005"
                     /old_locus_tag="QDX28_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_085439976.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYLEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEISPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLEKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSVREAERRREEAEAEARRKEQEHEARLKRHDNHCLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRVGEFTVWGDELAHYPQSVHDQVNVYLSPGGAVMVSDIREG
                     MPESHETLARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDVTAYAVPGQLMPVVFSTERNSRKLNLLLCKGPEEMLVGFVRQEDGLRPV
                     LVLPSPDYSHLMVSTITENGIHLAGYGEAINRDADTPYPPEPHLMQFRLKGCQDTLFAA
                     INKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            complement(2722..3909)
                     /locus_tag="QDX28_RS00010"
                     /old_locus_tag="QDX28_27130"
     CDS             complement(2722..3909)
                     /locus_tag="QDX28_RS00010"
                     /old_locus_tag="QDX28_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element IS91 family transposase"
                     /protein_id="WP_000937614.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVTESVTKIMACGTTL
                     MGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTL
                     PCQYWSLVFHNRWLLAEMSRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHIHLS
                     TTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPELVIPDELAVEGNSKRDWNR
                     FLDTHYRRGWNVNISRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHR
                     TKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEEVVYVITETVRKTA
                     MQIRWRGMYQRLLKVDPLKCILCGSQMRFTGLKRGYRLAELVLMHERLARQQVCG"
                     /name="IS91-like element IS91 family transposase"
     gene            complement(3909..4274)
                     /locus_tag="QDX28_RS00015"
                     /old_locus_tag="QDX28_27135"
     CDS             complement(3909..4274)
                     /locus_tag="QDX28_RS00015"
                     /old_locus_tag="QDX28_27135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000124098.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDAEVLRFDNF
                     DDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVTPPV"
                     /name="hypothetical protein"
     gene            4293..5921
                     /locus_tag="QDX28_RS00020"
                     /old_locus_tag="QDX28_27140"
     CDS             4293..5921
                     /locus_tag="QDX28_RS00020"
                     /old_locus_tag="QDX28_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_284453042.1"
                     /translation="MTLSFFITVIPDHDKPQPPGCLLGGNGGASDGSGWSSENNPWGGG
                     SGSGVHWGGGSGHGNGGGNGNSGGGSNSSVAAVAFGFPALAAPGAGTLGIAVSGEALSA
                     AIADIFAALKGPFKFSAWGIALYSILPSEIAKDDPKMMSKIVTSLPAETVTNVQVSTLP
                     LDQATVRVTKRVTDVVKDKRQHIAVVAGVPMSVPVVNAKPTRTPGVFRASFPGVPSLTL
                     STVKGLPASTTLPRGITEDKGRTASPAGFTFGGGSHEAVIRFPKESGQKPVYVSVTDVL
                     TSAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELNQANKDVARNQERQAKAVHV
                     YNSRKSELDAANKTFADAKAEIKRFERFAREPMAAGHRMWQMAGLKAQRAQTDVNNKKA
                     AFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKPRKGVKDYGHDYHPAP
                     KTEEIKGLGELKKAPKKTPKQGGGGRRDRWIGDKGRKIYEWDSQHGELEGYRASDGEHI
                     GAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5931..6188
                     /locus_tag="QDX28_RS00025"
                     /old_locus_tag="QDX28_27145"
     CDS             5931..6188
                     /locus_tag="QDX28_RS00025"
                     /old_locus_tag="QDX28_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_042090694.1"
                     /translation="MGLKLNLTWFDKKTEDFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGGFDVKKSWVPLLQPYFKNKIEVDKYWYQISFDYRDGNW"
                     /name="cloacin immunity family protein"
     gene            6272..6415
                     /locus_tag="QDX28_RS00030"
                     /old_locus_tag="QDX28_27150"
     CDS             6272..6415
                     /locus_tag="QDX28_RS00030"
                     /old_locus_tag="QDX28_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_042090693.1"
                     /translation="MKKITGIILLLFAAIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     gene            6912..7805
                     /locus_tag="QDX28_RS00035"
                     /old_locus_tag="QDX28_27155"
     CDS             6912..7805
                     /locus_tag="QDX28_RS00035"
                     /old_locus_tag="QDX28_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_052988103.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(7776..8000)
                     /locus_tag="QDX28_RS00040"
     CDS             complement(7776..8000)
                     /locus_tag="QDX28_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052984293.1"
                     /translation="MHHRTPLRFLAHSLATLGHETAASATTSIMLKATPLRGRTEEIAR
                     KIAAQKDAKRALLSDKAYLVSFCEIWITF"
                     /name="hypothetical protein"
     gene            complement(8031..8297)
                     /locus_tag="QDX28_RS00045"
                     /old_locus_tag="QDX28_27160"
     CDS             complement(8031..8297)
                     /locus_tag="QDX28_RS00045"
                     /old_locus_tag="QDX28_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091750.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
                     /name="hypothetical protein"
     BGC             1..8475
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2..359)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.164"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(8301..8475)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.455"
                     /coverage="99.0"
                     /evalue="1.59e-73"
     Typing          7018..7805
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.29"
                     /coverage="88.0"
                     /evalue="0.0"
     Typing          2..2217
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.805"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcatctcag catgaaaatc atttcgcgaa aaaacggcta cagtgccgtt
       61 gcttctgctg cctaccgttc cggctctgtc atacccgatg accgtaccgg attaacccac
      121 gattacaccc gtaaacgcgg cgttgatgat gcggtcattc tcacccctgc gaatgcaccg
      181 tcctggtgtg gtgaccgttc cgttctctgg aatgcggtcg agaaagccga acagcgccgg
      241 aactcccagc tggcaaggga gattgaactc gccattcccc gtgagatttc ccgcgaggcc
      301 gcacgggaaa ccgttctcgc ttttgtccgg gaaaactttg tcagtcgggg catgattgcc
      361 gatgtggcgt tccatcacat ggaccggacc aacccccatg cacacatcat gctgaccacg
      421 agagctgtcg gggaaacggg attcgcagga aaggtcaggg actggaacga ccgggcactc
      481 gccgagacgt ggcgcgcatc atgggctgac catgcgaaca gggcactggc gaacgccggt
      541 tacctggaag agatagacca tcgttcatac gagcgtcagg gactggagaa agcgcccggc
      601 cttcacctcg gaaaggctgc ctgtgcgatg gaaaaacggg ggatggaaac agaacgcggt
      661 gagcagaacc gtctgattaa cagccttaac ctggaaatac aggtttcccg cacgcagctg
      721 gctctcagga cggttcagga agcgcagcgt aagcgggaac tcagcgatgc cgcacgtcgt
      781 gcagctgaag cccttaacct gaccattccc gctgcgaatg cctcagcgga taccctgcgg
      841 gaattcattg ccacgctgcc gcaggaatgc gggaacgcgt gggagataag cccggagttc
      901 ctggcgatga gcgggaaggt gaacgacatc gaacgtgagg ggaatgcgct gctgaaagag
      961 caggccattc tcgaaaagga gatgaccgga ctcaaaaaag cacgccctgt cgcgtccctt
     1021 ctgtcagaga ttcccctgat gacatgggct gaaccggaat accgcaaaag acaactccgt
     1081 ttctggaaac tcgagaaaca gattgaatct cttcgccgca cctacagggc tgtgaaagaa
     1141 cgggacattc ccgcccgtcg tcaggccttt gaaacgcagt ggaatacgtg gattgcgccg
     1201 ggaatggcag agctgaaaga aaagctgtca gtacgggaag cggagcggcg cagggaggaa
     1261 gccgaagcgg aagcgcgccg gaaggaacag gagcatgagg cgcggctgaa acgccatgat
     1321 aaccactgtc tgagccgtga aacggcatta gtcggggtta ttacggagct ggggcgtgcc
     1381 agagagccgg gaacgggcag gataacccgc tacatgatgc tgagtaacag agtcggagaa
     1441 ttcacggtat ggggtgatga gctggcgcat tatccccaga gcgttcatga ccaggtgaat
     1501 gtttacctgt cgcctggcgg ggcagtgatg gtctcggata tacgtgaggg aatgccagaa
     1561 tctcatgaga cgctggcgcg gcctgagcgt gtgagaatgt attccggtgc gacggtccgg
     1621 catgtactgg aacagatgcg ccaggggtgg ccctcttacg gttttccggc gctgccgcat
     1681 cactggccgg ataattttta tttcagcgac gaccgcaggc ccgtagcctc tccgctgccg
     1741 tcagcgcacc gggtggacgt caccgcttat gcggtaccgg ggcaactcat gcccgttgta
     1801 ttttcgacag agcgaaacag caggaagctg aatctgctgt tgtgcaaagg gccggaggaa
     1861 atgcttgtcg gatttgtgcg ccaggaggac gggctgcgtc ccgttcttgt gcttccgtcg
     1921 ccggattaca gtcatctgat ggtcagcacc atcacagaga acgggataca cctggcaggc
     1981 tacggtgagg ccataaaccg tgatgctgat actccgtacc caccggaacc gcacctgatg
     2041 cagttccggc ttaagggctg tcaggacacg ctttttgctg ccatcaacaa accggaagag
     2101 atgccggatt atctcttccg tcaactcggt tttaatcaga cctggcatga gtggaagcgg
     2161 gacgaacagc acaggcaaca acaacgccgc cccgggcact tccggggtat gagtatgtga
     2221 tattcggggc cgcaccccgg accccgccaa cacatcacgg gccacaaaaa attttgtggc
     2281 ccgtgatgtg ttttctaagt gttatccctc ctgatttcta aaaaattttc cacctgaact
     2341 tgacagaaaa aacgatgacg ggtacttttt gatctgtaca taaaaccagt ggttttgtgt
     2401 acagtattat ctatgtaatt aattgtttta acgcttaaaa gagggacttt tatgagcggt
     2461 ggagatggac gtggccacaa cagtggcgca cataacacag gtggtaacat taatggcggc
     2521 cctacggggc ttgatcgata ggaatttaaa tccccaaaag actaaaaaag caccacaaaa
     2581 cggatgtttc ttcaacaccc cttgtgcacc atatgagcgg aaacgactat taaacaggat
     2641 gacactgatt gatacagggt atgaatggcg gttttttgct ccgtttccgg taaaatggac
     2701 gcaacttccc ctctgcggct ctcagccgca cacctgctgt cgtgccagtc gctcatgcat
     2761 caggaccagc tctgccagtc ggtaaccccg cttcagcccc gtaaaacgca tctgacttcc
     2821 gcacagaatg cacttcagcg ggtcaacctt cagtaacctc tgatacatcc ctctccacct
     2881 gatttgcatc gccgtttttc tcaccgtctc cgttatgacg tacacaactt cttccagtaa
     2941 gcggcgcttc accggactca ggaaaccgta gtacctcacc atacggaacc ccttatctgc
     3001 tacatgccag gagaaccttt ccatgaactc atctccactc atcaacaggt attcttcccg
     3061 ttttgtacgg tgactgttgt aacgcagacc gatttcatcc tgaccggcat aatgctccag
     3121 ccgactcatc ggcaccggtg gctttttcag gtaagagccg aagtacaccg ccacatgtgt
     3181 ggcgttatcc atcaccctgg atatgttgac attccagccg cggcggtaat gcgtgtccag
     3241 gaagcgattc cagtcccgtt tgctgtttcc ttccactgcc agttcatccg gtattaccag
     3301 ctccgggtat ttccgggaca gtagccgcgt tatccggtaa cgccacatgc tcatcacctt
     3361 acgggcgtaa aaatgaagat ttttccaggt gtggcccgac gtcacaccac cggcagttgt
     3421 cgataaatgg atatgcggat gccactgctg gtcacgcccc catgtgtgga tcaccgtgaa
     3481 tatccccggc tccacatctg tctgatggca gatttccagt atcacatccg ctgcaatgcg
     3541 gctcatctct gccagtaacc accggttgtg gaacaccagg gaccagtact ggcagggaag
     3601 tgtgaacaca atatgctgcc acgggcagtc ggggaccagg ctcagcagat actgtatcca
     3661 ctgtgcgcca gccttcaccc cgcagtgcgg acaggagcgg cttttacacc ggaagcagac
     3721 ctttttggtg tggcaacagt ccggtgacga acagcaccat tgcgtgtagc ccatcagcgt
     3781 ggtcccgcat gccatgattt ttgtcactga ctcagtcacc accggacgca ctgaaccttc
     3841 cggctgtttc tccagccagt taagccagcg gttaccctgc tgaaaaatat cggcaaaacg
     3901 gggaagcatc agacgggtgg ggtaacgcca tccggccagt gaactgtgcc gcattccggg
     3961 cagtacatac caccggcact gataccggca agaatggttg caaactcccg ctccgtacag
     4021 cgggctattt ccggataccc ttcgtcatcg acacgaacaa accagaagac cagctttttg
     4081 tttcctgcat ccagaaagaa cggaataccc aggtcagcgc agcactcaac ggcatcgtca
     4141 aaattatcaa agcgcagaac ttcagcgtct tcttcgtcaa aaaaatcatc cggatgaagt
     4201 ttcacaacat agcggggaag tttgcttctt tgtggtgcgg gtttgcgttt acggggttta
     4261 gctgaacggg ccatataact acctgaaaga caatgacatt atcttttttt ataacggtaa
     4321 ttccagacca tgacaagccg cagccgccag gctgcctact cggtggaaat ggtggggctt
     4381 ctgacggctc cggatggagt tcggaaaata acccatgggg tggcggttcc ggtagtggtg
     4441 ttcactgggg aggtggctcc ggccatggca atggtggggg gaatggtaat tccggtggtg
     4501 gcagcaactc atccgtagca gcagtggcat ttggttttcc tgctttggca gctcctggtg
     4561 ccggaacact gggtatcgcc gtctctggtg aggctttatc tgcggcaata gcggatatct
     4621 ttgcagccct gaaaggcccg tttaaattca gtgcatgggg tattgcgctt tacagcattc
     4681 tgccatctga aatagcaaaa gatgacccga aaatgatgtc aaagattgtg acgtcattac
     4741 cggcagaaac cgtgacgaat gtccaggtca gtacacttcc gctggaccag gcaacggtca
     4801 gagtgacgaa gcgtgtaaca gatgttgtga aggacaaacg acagcatatt gccgtggttg
     4861 cgggtgtacc tatgagtgtg cctgttgtaa atgctaaacc aacacgtact cctggtgtat
     4921 ttcgcgcatc atttcctggt gtcccttctc tgacacttag cactgtcaaa ggccttccgg
     4981 cgtcaacaac ccttccccgt ggtattacgg aggataaagg ccggactgcc tctcctgcag
     5041 gatttacctt tggtggtggt tcacatgaag cggtgatccg ttttccgaaa gaaagtgggc
     5101 agaagccggt ttatgtatca gtaacagatg ttcttacctc tgcacaggta aaacaacgtc
     5161 aggatgaaga gaatcgcctc cagcaggaat ggaatgacac acatccggta gaagtagctg
     5221 aacgcaatta tgaacaggct cgcgcagaac tgaatcaggc taataaggat gttgccagaa
     5281 atcaggaacg acaggctaaa gctgttcatg tttataattc gcgtaaaagt gaacttgatg
     5341 cagcgaataa aacttttgct gatgcaaagg ctgaaataaa gcgattcgag cgatttgctc
     5401 gagaaccaat ggctgctggt cacagaatgt ggcaaatggc agggcttaag gcccagcggg
     5461 cacagacgga tgtaaataat aagaaggctg catttgatgc tgccgcaaaa gagaagtcag
     5521 cagcagatgc tgcattgagt tctgcgatgg aaagcaggaa gaagaaagaa gataagaaaa
     5581 ggagtgctga aaataaatta aacgaggaaa aaaacaagcc tcgcaaggga gttaaagatt
     5641 acggtcatga ttatcatccc gctccaaaga ccgaagaaat aaaggggttg ggggaattaa
     5701 aaaaagcacc taaaaaaaca cctaaacaag gtggtggtgg tagacgtgac cgctggattg
     5761 gtgataaagg ccgtaagatt tatgaatggg actcccagca cggtgagctt gaagggtatc
     5821 gtgccagtga tggcgaacac atcggggcat ttgacccaaa aacgggtaaa caaattaaag
     5881 gtccggatcc gaaagggcga aatattaaaa aatatcttta agaggtaagt atgggactta
     5941 aattaaattt aacttggttt gataagaaaa ctgaagattt taaaggggaa gagtattcta
     6001 aagactttgg tgatgatggt tctgtcattg aaagtcttgg aatgccttta aaggataaca
     6061 ttaataatgg cgggtttgat gttaaaaaat catgggttcc gttgttgcag ccatatttta
     6121 aaaataaaat tgaagtagat aagtactggt atcaaatttc atttgattat cgggatggta
     6181 actggtaaca aaaaattatc aaggaagagt tgaaatacag acgtccggtg ttcagggatg
     6241 aatgccggat tctttaaata caggggaaag tatgaaaaaa ataacaggga ttattttatt
     6301 gctttttgca gccattattc tggctgcttg tcaggcaaac tatatccgtg atgttcaggg
     6361 cgggactgta tcaccgtcat caactgctga actgaccgga gtagaaacgc agtaacctgc
     6421 aatcctcatt tgacaagaac aaagcgtgtc aggctgatcc tgacgcgctt ttttttgaag
     6481 tttcacaaaa attccatgcg ggagatgaga tctaaaatcc catgcagaac cactcatcca
     6541 gggggagaaa acttgtcatt ttgacccgtt cggtgttcag aacacacgaa accgatcgcg
     6601 catcgctttc gtgcatagtt atgcagcgcc ctaaaaacga ttctgacgca tttttctggt
     6661 tcagcctggt gtttccttgt ctttttgcgt tttttgcgtc agaaagcgat tgagggcgtt
     6721 ttatggggtg cgtacaacgg gaattatggt aaatggatcg ggttttcggg aaggttcgac
     6781 aggatttgcc gttgggtgta gtgtaagcga ctgaaaaaca aacgcccctg aaatcatgtc
     6841 cagatccggc aagattcaac atgaaataca gagggcgtgt aggtcgcata acccggagag
     6901 aatcgtaaca catgagcgcc gcgcttcaat acttcgacga aaatttaccc caccgtccct
     6961 atcacacgga tgatctcgct tttggtcttc gcatctccgg caaagggcgt gcgcttcttg
     7021 cgcggtacat acagcagaac cagcctcatg cgcagttctg gctggttttt gacgttgacc
     7081 gcgagggggc tgcgattgac tggagcgacc ggaacgctcc cgcgccgaac atcaccgtaa
     7141 aaaatcctgt gaacggtcat gctcacctgc tctacgcgct caatatcgcc gtgagaactg
     7201 cgccggacag ctcggttaag gcgctgaaat acgccgctgc gattgaacgt tcgctgtgtg
     7261 agaaactttg tgctgatgtg aactacagcg gactgatttg taaaaatccg ttccacctgg
     7321 aatggcaggt gatggagtgg cgagaggaag cctataccct cgatgaactg gctgattatc
     7381 tcgatttgag cgcctcagcg cgccgtagca tcgataaaca ttacgggatg gggcgaaact
     7441 gccacctgtt cgaaatgacg cgcaaatggg cttacagggc gattcgtcag ggctggcctg
     7501 tattctcaca atggcttgat gccgtgatcc agcgtgtcga aatgtacaac gcatcgcttc
     7561 ccgttccgct ttctccggct gaatgtcggg ctattggcaa gagcattgcg aaatatacac
     7621 acaggaaatt ctcaccagag ggattttccg ctgtacaggc cgctcgaggt cgcaagggcg
     7681 gaactaaatc taagcgcgca gcagttccta catcagcacg ttcgctgaag ccgtgggagg
     7741 cattagggat cagtcgagcg acgtactacc gaaaattaaa atgtgatcca gatctcgcaa
     7801 aatgagacca gataagcctt atcagataac agcgcccttt tggcgtcttt ttgagctgcg
     7861 atctttcttg cgatttcctc cgttctgccc cgtaatggcg tggctttcag catgattgag
     7921 gtggtagcgc tcgccgccgt ctcatgaccg agcgtagcga gcgaatgagc gaggaagcgc
     7981 aaaggcgtcc ggtggtgcat gtggcactta cgcgccgggg tttctccgtt ctacggtttc
     8041 gcaggtggtt tgggtagctt ctccagctcg ttaatcagag gttgtatccg gttcacatcg
     8101 gcctgtcttg tgacttcctt tcgcaggaac tggagcagga acgcacgcag ttgcgcttct
     8161 tccggcctcc gtaccctcgc cagcatggca gcccccacaa tgactttttg cgccgtgtcc
     8221 aggctccggc tcttcgcctt caggcgctgc aatctggcct cagcttcggc aatcttctgt
     8281 tcgagtgttc tgctcatttt gtgactccgt gcgcggtgaa aaatcgcatt ttagcgcgtc
     8341 actggtagtt gcaaaactga actggcataa tgcacggcaa atcacgaagt gcgcacttat
     8401 acaatctcca ctccgtttcg attgtgtgcg ccctgcgggg ctgaaagaaa acggcaaaaa
     8461 ggcattacgg aagaa
//
