LOCUS       NZ_CP122706             4090 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli strain ETEC4061 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP122706
VERSION     NZ_CP122706.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549638
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 315x
            Sequencing Technology        :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_030166005.1-RS_2024_06_19
            Annotation Date              :: 06/19/2024 11:43:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122706.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC4061"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            43..2196
                     /gene="mobQ"
                     /locus_tag="QDX28_RS00005"
                     /old_locus_tag="QDX28_26830"
     CDS             43..2196
                     /gene="mobQ"
                     /locus_tag="QDX28_RS00005"
                     /old_locus_tag="QDX28_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_284447784.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPEDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGESGLAALRSQL
                     LKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQHPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREAPLFAVVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRPDRGWSQGM"
                     /name="mobQ"
     gene            complement(2438..3139)
                     /locus_tag="QDX28_RS00010"
                     /old_locus_tag="QDX28_26835"
     CDS             complement(2438..3139)
                     /locus_tag="QDX28_RS00010"
                     /old_locus_tag="QDX28_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3672..3962)
                     /locus_tag="QDX28_RS00015"
                     /old_locus_tag="QDX28_26840"
     CDS             complement(3672..3962)
                     /locus_tag="QDX28_RS00015"
                     /old_locus_tag="QDX28_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001514113.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     Typing          complement(44..398)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2440..3139)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..2187
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.827"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcgccctg cggggctaaa agaaaacggc aacaacgaca cgatggcaat ttatcatctc
       61 agcatgaaga ttctttccag aagcaagggc tacagcgccg ttgcttctgc tgcctatcgt
      121 gccggagaaa aaatactgga tgagcgcacc ggagtgattc acgactacac ccgcaaaaac
      181 ggtgtagcca gcgcagtgat tctgacgcca gcaaacgccc cagcatggtg tgcgaaccgc
      241 gccgaactct ggaatgctgt tgaaaaagcc gagcggcgga aaaactcaca acttgcgcgg
      301 gagttcgaac tcgccatccc ccgtgaactg gcgcaggatg ccgcacggga aacagttctc
      361 aacttcgtaa gggaaaactt tgtcagtcgc ggcatgatcg ccgacgtggc ctttcacaat
      421 ctgggcgggt caaacccaca cgcgcacatc atgctatcca tgagggcaat cacgcctgac
      481 ggcttcgggg agaaagtccg tgagtggaat gactggactc acgccgaaac atggcgggga
      541 tcatgggcag accatgcgaa cagggcgctg gcgaacgccg gttatcagga agagatagac
      601 catcgttcat acgagcgaca gggggttgag aaaacgcctg gcattcacct cggaaagtcg
      661 gcctgtgcga tggaaaagtg gggcatcgaa acagaacgcg gcgaacagaa tcgcctgatt
      721 aacaggctta acctggaaat acaggtttcc cgcgacgaac tcaggaacag tgagctgaca
      781 gagcccgcac gccgtatggc tgaccttctg aatattgagc ttcctgacaa cgcaacttcg
      841 tacatactgc atgacatcat cgaagcgttg ccggaggaca gtaacgcggc atggaaactc
      901 accagtaacg ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag
      961 ctgaacaccg aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg
     1021 ttttcgtccg gtttttcccg tattcccctg atgcaatggg ctgcaccgga ataccgtagt
     1081 gagcagggaa aaatccagtc tctgagccag caaatggaaa aattgcgaca gcattaccgg
     1141 aaggtggaga aacaggacat tcccgcgagt cagacggcat ttgaaaaaca gtggcagcag
     1201 tggggggaat ccggccttgc agctctcaga tcccagttgc ttaaacgtga agaggaattg
     1261 cgccggaagg agctggagga tacagaacgt agtcgcgcgg agcagctcag aaaaaatgac
     1321 aatgccgtta tggattcagg ggtgctgaga gcagtcgtta cggggtatgg cgaggctccc
     1381 atgcctggta acggggaaat gacctgttac cttctgcttc acaaccggaa cggagaatat
     1441 acgctctggg ggaacgagct ggagaaatac cgcaccagga tctttgagag tgttgattta
     1501 atgcgtgaca ggagtggtta tatctgtgac cgaagcgaaa tcggtcaaca tccaccactg
     1561 caacgtgttt attcctctgc gacctttgag caacttctga cgcaggtttg ccagacatgg
     1621 ccgcaacata cccgcaatct ccgccaaccg aagacatggc cggagtcttt ctgtcttgga
     1681 gaagaccgcc agccagccat gccatcactg gcagcaagga aggtggattt tacgcaggga
     1741 cgtctgccac cgacgctgat gccggtcatg agttctgtcg accgggaaac ccgccagctt
     1801 cagcttttgc tggtcatggg cgtggatgac tctctgggcg gtgttgtccg gcttaatggt
     1861 acgctgtatc ccgcgtttgc cgttccctca gccgataaca gccagctggt catcagtgca
     1921 ctgaccgata aggggttacg gtatgcaggg tatggtgttg ccgtaaatca cgatgcagac
     1981 agccacatat cacctgcgcc ggaacttatg gagtttcatc tgaaaacccg tgaggcaccg
     2041 ctttttgctg tcgtcaacac accagaaaaa cagccggatc atctgttccg ctcgctgggt
     2101 ttcaatcgta cctgggacga atggcggcgc gaggaagatg cacgaacaca cgccaccgag
     2161 aggagacctg atcgcggttg gtcacaggga atgtgaacaa tattttgttt tgtttaacat
     2221 cgtgttatgt tcgtcgatgt tcatcgacag ggatgaatgc aagaagcaca ctcacatagc
     2281 gcaaacatag ttttgttttt ccgcccccaa aaattggggg ttcttttttt gctgcatcag
     2341 agcgtttttg acgcaacttt tcacttcaat agtaccttcg cattcctgac acaagaaaaa
     2401 cgcgacagtg agcgttttag gtgccttaaa atcgattcta gatcgtcatc ttgatctgtt
     2461 tgtcttcctt gaagcgaaaa tgcagggcta caaccgtgcg ccccttcttg atcgtctcaa
     2521 gcgttacagc caagtcgctt ttttgattca gttcgtcaac gcaaggtctt aataattgct
     2581 ggttcagaga cttatatgtt tgatatttat gctctatatc aagcattgag cgaaaatcat
     2641 cgagggcgat aatacgttcc cctgtgctgc ggaattgctg gagaagttcg tatattctta
     2701 tgctgtaact tcttgataaa ttggagatgt tcttaattac aactctagta aattgcccct
     2761 tcaactgtgt cagataaggc atgaccgcat ctgaaaaagt gatttgggct ttgccctcac
     2821 ctttagcgta ctgtgcccga tactggatcc agcggaactc ctcgcgcttc tcatcgtcct
     2881 taaggatgat cgacctgtcc cacaaacgat caattgcttc ctgtagctga acctcggcat
     2941 ttttccgccc catatcaggg aatgagtcga aatactcttc ggcggtaaca gtcatggtct
     3001 tttgcaattc agtggattct gcatcagaac ccgacttcaa tcgaccaatg caaagtaaca
     3061 agaatcgctg ttcctgaaga gtcaatttat agctggcctc aaccagtgca ttggatttgt
     3121 aggcaatcag atccgacatt gtaaatccct caacctaagg taatgagtaa aatataaaca
     3181 ttcctttagg taaaggcaat atgatttaat tttaatatcc cttttcctgt gaatattacc
     3241 ttaaatcata ttgcctttac cttaaatcat attgcctatc caccttaaat catattgcct
     3301 ttaccttaaa ttatattacc tatagtcttc taaactcttg aatttcataa gaaaattcga
     3361 ccctaaaaca agtattaaaa acaagatctt aaaaacaaga tctaaaaact taacggcgag
     3421 cctgtggaaa acttggggtg ttcaaaacaa aaaatcatga aaaacaacag gttacaaaaa
     3481 agcaggttcg caagctttaa caaaaccccg ctcaaccctg tggaaatcca gtattcatgc
     3541 gggttcagcg catatcacct gtcgaaaaac cgtgcgtatg tgctcaatct ggcgacggat
     3601 tcacttccgc ttcctcgccc actcgctcgc taacgctcgg tcgctggggc tgcggcgagc
     3661 ggacggtgca ttcacgcctg ttggtcgttt tctgacactt ctttggggac tggttttggt
     3721 aacgcgttca gttcgtcaat caacggctgt atcctgctca catcagcctg ccgtgttacc
     3781 tctgtcttca ggagatgaag caaccaggct cgtagttgtg tttccccagg cttcctgacc
     3841 tttgccagca atgccgctcc cacaatgacc ttctgcgctg tgtccaggct cctgctcttc
     3901 gccttcagac gctgcaatct ggcctcagca tcggcaatct tctgttcgag tgttctactc
     3961 attccgtaac tccgtgcgcg gtgaaaaatc gcattttagc gcgtcactgg tagtttaaaa
     4021 actgaactgg cataatgcac agcaaatcac gaagtgcgca cttatacaaa ctctccttcg
     4081 tcgagtttgt
//
